Restriction map for pF1KSDA0359
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BbrPI       BbvCI       BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FbaI        FseI        
FspI        HgaI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         638  CGCG                 CG^CG
AcsI         1069  GAATTC               [GA]^AATT[CT]
AcyI         1390  GGCGCC               G[GA]^CG[CT]C
AfaI          216  GTAC                 GT^AC
AocI         2186  CCTCAGG              CC^T[ACGT]AGG
ApoI         1069  GAATTC               [GA]^AATT[CT]
AspI          244  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2222  CTCGGG               C^[CT]CG[GA]G
BalI          941  TGGCCA               TGG^CCA
BamHI        2170  GGATCC               G^GATCC
BanI         1390  GGCGCC               G^G[CT][GA]CC
BbeI         1390  GGCGCC               GGCGC^C
BbsI          537  GTCTTC               GAAGAC(2/6)
BcgI         1503  GCAGAAACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         537  GTCTTC               GAAGAC(2/6)
BsaHI        1390  GGCGCC               G[GA]^CG[CT]C
BsgI          672  GTGCAG               GTGCAG(16/14)
BsmBI         223  GAGACG               CGTCTC(1/5)
BsmI          195  GAATGC               GAATGC(1/-1)
BsoBI        2222  CTCGGG               C^[CT]CG[GA]G
BsrFI         763  ACCGGC               [GA]^CCGG[CT]
BstAPI       2059  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        331  GGTGACC              G^GT[ACGT]ACC
BstPI         331  GGTGACC              G^GT[ACGT]ACC
BstUI         638  CGCG                 CG^CG
Bsu36I       2186  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        763  ACCGGC               [GA]^CCGG[CT]
Eco81I       2186  CCTCAGG              CC^T[ACGT]AGG
EcoNI         508  CCTGACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       331  GGTGACC              G^GT[ACGT]ACC
EcoRI        1069  GAATTC               G^AATTC
EcoT22I      2102  ATGCAT               ATGCA^T
Hin1I        1390  GGCGCC               G[GA]^CG[CT]C
HincII        241  GTTGAC               GT[CT]^[GA]AC
HindII        241  GTTGAC               GT[CT]^[GA]AC
KasI         1390  GGCGCC               G^GCGCC
MluNI         941  TGGCCA               TGG^CCA
MscI          941  TGGCCA               TGG^CCA
MspA1I       1102  CAGCTG               C[AC]G^C[GT]G
MvnI          638  CGCG                 CG^CG
NarI         1390  GGCGCC               GG^CGCC
NsiI         2102  ATGCAT               ATGCA^T
PflFI         244  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         2074  CTGCAG               CTGCA^G
PvuII        1102  CAGCTG               CAG^CTG
RsaI          216  GTAC                 GT^AC
SapI         1629  GCTCTTC              GCTCTTC(1/4)
SexAI         413  ACCTGGT              A^CC[AT]GGT
SfcI         2074  CTGCAG               C^T[GA][CT]AG
SfoI         1390  GGCGCC               GGC^GCC
Tth111I       244  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BanII         493  GAGCTC               G[GA]GC[CT]^C
             1723  GAGCTC              
BfaI          844  CTAG                 C^TAG
             1705  CTAG                
BglII         437  AGATCT               A^GATCT
              732  AGATCT              
BsaI         1555  GAGACC               GGTCTC(1/5)
             1908  GAGACC              
BspMI        1355  ACCTGC               ACCTGC(4/8)
              117  GCAGGT              
EaeI          941  TGGCCA               [CT]^GGCC[GA]
             1882  CGGCCA              
HaeII        1390  GGCGCC               [GA]GCGC^[CT]
             1694  AGCGCC              
HapII         764  CCGG                 C^CGG
             2007  CCGG                
HindIII       886  AAGCTT               A^AGCTT
             1639  AAGCTT              
HpaII         764  CCGG                 C^CGG
             2007  CCGG                
MaeI          844  CTAG                 C^TAG
             1705  CTAG                
MaeIII        332  GTGAC                ^GT[ACGT]AC
              547  GTCAC               
MspI          764  CCGG                 C^CGG
             2007  CCGG                
NcoI          314  CCATGG               C^CATGG
             1406  CCATGG              
NspI          573  GCATGT               [GA]CATG^[CT]
             1997  ACATGC              
PpuMI        1043  AGGACCC              [GA]G^G[AT]CC[CT]
             1352  AGGACCT             
SacI          493  GAGCTC               GAGCT^C
             1723  GAGCTC              
StuI          505  AGGCCT               AGG^CCT
             2146  AGGCCT              
Tsp45I        332  GTGAC                ^GT[GC]AC
              547  GTCAC               
XcmI          903  CCAAGATTCCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              926  CCAAGACTGTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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