Restriction map for pF1KSDA0359
 (Linear) (Six-base) MAPSORT of: KIAA0359.seq  Check: 2447  from: 1  to: 2241



KIAA0359.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     108    2241 
   Size:        108    2133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    1627    2241 
   Size:       1348     279     614

  Fragments arranged by size: 

                1348     614     279
 
AleI CACnn'nnGTG

Cuts at:      0     517     707    2241 
   Size:        517     190    1534

  Fragments arranged by size: 

                1534     517     190
 
ApoI r'AATT_y

Cuts at:      0    1069    2241 
   Size:       1069    1172
 
AvaI C'yCGr_G

Cuts at:      0    2222    2241 
   Size:       2222      19
 
BamHI G'GATC_C

Cuts at:      0    2170    2241 
   Size:       2170      71
 
BanI G'GyrC_C

Cuts at:      0    1390    2241 
   Size:       1390     851
 
BanII G_rGCy'C

Cuts at:      0     497    1727    2241 
   Size:        497    1230     514

  Fragments arranged by size: 

                1230     514     497
 
BbeI G_GCGC'C

Cuts at:      0    1394    2241 
   Size:       1394     847
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530    2241 
   Size:        530    1711
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1492    1526    2241 
   Size:       1492      34     715

  Fragments arranged by size: 

                1492     715      34
 
BfrBI ATG'CAT

Cuts at:      0    2104    2241 
   Size:       2104     137
 
BglII A'GATC_T

Cuts at:      0     437     732    2241 
   Size:        437     295    1509

  Fragments arranged by size: 

                1509     437     295
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     302    2241 
   Size:        302    1939
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     481     651    2241 
   Size:        481     170    1590

  Fragments arranged by size: 

                1590     481     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1282    1495    1879    2241 
   Size:       1282     213     384     362

  Fragments arranged by size: 

                1282     384     362     213
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511    1078    1729    2241 
   Size:        511     567     651     512

  Fragments arranged by size: 

                 651     567     512     511
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1549    1902    2241 
   Size:       1549     353     339

  Fragments arranged by size: 

                1549     353     339
 
BsaHI Gr'CG_yC

Cuts at:      0    1391    2241 
   Size:       1391     850
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     509     539    1140    1170    1402    1432    2241 
   Size:        509      30     601      30     232      30     809

  Fragments arranged by size: 

                 809     601     509     232      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     362     463     889    1198    1201    1204    1207    1225 
   Size:        362     101     426     309       3       3       3      18

Cuts at:   1225    1324    1567    2166    2169    2241 
   Size:         99     243     599       3      72

  Fragments arranged by size: 

                 599     426     362     309     243     101      99      72
                  18       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     111    1100    1386    2002    2241 
   Size:        111     989     286     616     239

  Fragments arranged by size: 

                 989     616     286     239     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693    2241 
   Size:        693    1548
 
BsiHKAI G_wGCw'C

Cuts at:      0     497     632    1727    2241 
   Size:        497     135    1095     514

  Fragments arranged by size: 

                1095     514     497     135
 
BsmI GAATG_Cn'

Cuts at:      0     201    2241 
   Size:        201    2040
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     217    2241 
   Size:        217    2024
 
Bsp1286I G_dGCh'C

Cuts at:      0     497     632    1727    2241 
   Size:        497     135    1095     514

  Fragments arranged by size: 

                1095     514     497     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     108    1364    2241 
   Size:        108    1256     877

  Fragments arranged by size: 

                1256     877     108
 
BsrDI GCAATG_nn'

Cuts at:      0     927    1585    2036    2241 
   Size:        927     658     451     205

  Fragments arranged by size: 

                 927     658     451     205
 
BsrFI r'CCGG_y

Cuts at:      0     763    2241 
   Size:        763    1478
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2065    2241 
   Size:       2065     176
 
BstEII G'GTnAC_C

Cuts at:      0     331    2241 
   Size:        331    1910
 
BstYI r'GATC_y

Cuts at:      0     437     452     732    1661    2170    2241 
   Size:        437      15     280     929     509      71

  Fragments arranged by size: 

                 929     509     437     280      71      15
 
Bsu36I CC'TnA_GG

Cuts at:      0    2187    2241 
   Size:       2187      54
 
BtgI C'CryG_G

Cuts at:      0     314     327    1406    1895    2241 
   Size:        314      13    1079     489     346

  Fragments arranged by size: 

                1079     489     346     314      13
 
BtsI GCAGTG_nn'

Cuts at:      0    1170    1655    2120    2241 
   Size:       1170     485     465     121

  Fragments arranged by size: 

                1170     485     465     121
 
EaeI y'GGCC_r

Cuts at:      0     941    1882    2241 
   Size:        941     941     359

  Fragments arranged by size: 

                 941     941     359
 
EarI CTCTTCn'nnn_

Cuts at:      0     968    1073    1126    1175    1196    1636    1814    2187 
   Size:        968     105      53      49      21     440     178     373

Cuts at:   2187    2241 
   Size:         54

  Fragments arranged by size: 

                 968     440     373     178     105      54      53      49
                  21
 
EcoICRI GAG'CTC

Cuts at:      0     495    1725    2241 
   Size:        495    1230     516

  Fragments arranged by size: 

                1230     516     495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1282    1348    1495    1627    1879    2241 
   Size:       1282      66     147     132     252     362

  Fragments arranged by size: 

                1282     362     252     147     132      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     512    2241 
   Size:        512    1729
 
EcoO109I rG'GnC_Cy

Cuts at:      0     952    1044    1353    2241 
   Size:        952      92     309     888

  Fragments arranged by size: 

                 952     888     309      92
 
EcoRI G'AATT_C

Cuts at:      0    1069    2241 
   Size:       1069    1172
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     928    2241 
   Size:        928    1313
 
HaeII r_GCGC'y

Cuts at:      0    1394    1698    2241 
   Size:       1394     304     543

  Fragments arranged by size: 

                1394     543     304
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     451     483    1527    1559    1932    1964    2092    2124 
   Size:        451      32    1044      32     373      32     128      32

Cuts at:   2124    2241 
   Size:        117

  Fragments arranged by size: 

                1044     451     373     128     117      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     243    2241 
   Size:        243    1998
 
HindIII A'AGCT_T

Cuts at:      0     886    1639    2241 
   Size:        886     753     602

  Fragments arranged by size: 

                 886     753     602
 
KasI G'GCGC_C

Cuts at:      0    1390    2241 
   Size:       1390     851
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    1407    2241 
   Size:        255    1152     834

  Fragments arranged by size: 

                1152     834     255
 
MscI TGG'CCA

Cuts at:      0     943    2241 
   Size:        943    1298
 
MslI CAynn'nnrTG

Cuts at:      0     517     707    1454    2241 
   Size:        517     190     747     787

  Fragments arranged by size: 

                 787     747     517     190
 
MspA1I CmG'CkG

Cuts at:      0    1104    2241 
   Size:       1104    1137
 
NarI GG'CG_CC

Cuts at:      0    1391    2241 
   Size:       1391     850
 
NcoI C'CATG_G

Cuts at:      0     314    1406    2241 
   Size:        314    1092     835

  Fragments arranged by size: 

                1092     835     314
 
NsiI A_TGCA'T

Cuts at:      0    2106    2241 
   Size:       2106     135
 
NspI r_CATG'y

Cuts at:      0     577    2001    2241 
   Size:        577    1424     240

  Fragments arranged by size: 

                1424     577     240
 
PfoI T'CCnGG_A

Cuts at:      0    1073    1472    2241 
   Size:       1073     399     769

  Fragments arranged by size: 

                1073     769     399
 
PpuMI rG'GwC_Cy

Cuts at:      0    1044    1353    2241 
   Size:       1044     309     888

  Fragments arranged by size: 

                1044     888     309
 
PstI C_TGCA'G

Cuts at:      0    2078    2241 
   Size:       2078     163
 
PvuII CAG'CTG

Cuts at:      0    1104    2241 
   Size:       1104    1137
 
SacI G_AGCT'C

Cuts at:      0     497    1727    2241 
   Size:        497    1230     514

  Fragments arranged by size: 

                1230     514     497
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1636    2241 
   Size:       1636     605
 
SexAI A'CCwGG_T

Cuts at:      0     413    2241 
   Size:        413    1828
 
SfcI C'TryA_G

Cuts at:      0    2074    2241 
   Size:       2074     167
 
SfoI GGC'GCC

Cuts at:      0    1392    2241 
   Size:       1392     849
 
SmlI C'TyrA_G

Cuts at:      0     490    1093    1744    2241 
   Size:        490     603     651     497

  Fragments arranged by size: 

                 651     603     497     490
 
StuI AGG'CCT

Cuts at:      0     507    2148    2241 
   Size:        507    1641      93

  Fragments arranged by size: 

                1641     507      93
 
StyI C'CwwG_G

Cuts at:      0     314     912    1030    1049    1406    1559    2045    2138 
   Size:        314     598     118      19     357     153     486      93

Cuts at:   2138    2241 
   Size:        103

  Fragments arranged by size: 

                 598     486     357     314     153     118     103      93
                  19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     397    2241 
   Size:        397    1844
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     509     541     852     884    1140    1172    2241 
   Size:        509      32     311      32     256      32    1069

  Fragments arranged by size: 

                1069     509     311     256      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     247    2241 
   Size:        247    1994
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     910     933    2241 
   Size:        910      23    1308

  Fragments arranged by size: 

                1308     910      23
 

 Enzymes that do cut:

     AarI     AcuI     AleI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BfrBI    BglII     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI   BstEII
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NsiI
     NspI     PfoI    PpuMI     PstI    PvuII     SacI     SapI    SexAI
     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI
    BsiWI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoRV     FseI     FspI    FspAI     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     XbaI     XhoI
     XmaI     XmnI     ZraI
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