Restriction map for
pF1KSDA0316
(Linear) (Six-base) MAPSORT of: KIAA0316.seq Check: 5921 from: 1 to: 3966
KIAA0316.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3526 3966
Size: 3526 440
AccI GT'mk_AC
Cuts at: 0 1659 3966
Size: 1659 2307
Acc65I G'GTAC_C
Cuts at: 0 1179 3966
Size: 1179 2787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 350 1478 1936 3311 3458 3632 3764
Size: 41 309 1128 458 1375 147 174 132
Cuts at: 3764 3966
Size: 202
Fragments arranged by size:
1375 1128 458 309 202 174 147 132
41
AfeI AGC'GCT
Cuts at: 0 1300 3966
Size: 1300 2666
AgeI A'CCGG_T
Cuts at: 0 378 3966
Size: 378 3588
AleI CACnn'nnGTG
Cuts at: 0 3700 3966
Size: 3700 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2533 3081 3966
Size: 2533 548 885
Fragments arranged by size:
2533 885 548
AlwNI CAG_nnn'CTG
Cuts at: 0 425 1612 1827 3689 3874 3966
Size: 425 1187 215 1862 185 92
Fragments arranged by size:
1862 1187 425 215 185 92
ApaI G_GGCC'C
Cuts at: 0 1328 3966
Size: 1328 2638
ApoI r'AATT_y
Cuts at: 0 2136 3190 3966
Size: 2136 1054 776
Fragments arranged by size:
2136 1054 776
AseI AT'TA_AT
Cuts at: 0 368 3966
Size: 368 3598
AvaI C'yCGr_G
Cuts at: 0 75 345 1837 2491 2718 3966
Size: 75 270 1492 654 227 1248
Fragments arranged by size:
1492 1248 654 270 227 75
BanI G'GyrC_C
Cuts at: 0 99 1179 3111 3966
Size: 99 1080 1932 855
Fragments arranged by size:
1932 1080 855 99
BanII G_rGCy'C
Cuts at: 0 1328 2625 3399 3637 3795 3966
Size: 1328 1297 774 238 158 171
Fragments arranged by size:
1328 1297 774 238 171 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 1738 2699 3197 3966
Size: 47 1691 961 498 769
Fragments arranged by size:
1691 961 769 498 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 17 51 3966
Size: 17 34 3915
Fragments arranged by size:
3915 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 1522 3966
Size: 1522 2444
BlpI GC'TnA_GC
Cuts at: 0 1828 1877 3966
Size: 1828 49 2089
Fragments arranged by size:
2089 1828 49
Bme1580I G_kGCm'C
Cuts at: 0 1328 2561 3966
Size: 1328 1233 1405
Fragments arranged by size:
1405 1328 1233
BmgBI CAC'GTC
Cuts at: 0 1398 3459 3966
Size: 1398 2061 507
Fragments arranged by size:
2061 1398 507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 726 3966
Size: 726 3240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 2065 2090 2348 3262 3966
Size: 766 1299 25 258 914 704
Fragments arranged by size:
1299 914 766 704 258 25
Bpu10I CC'TnA_GC
Cuts at: 0 3226 3423 3966
Size: 3226 197 543
Fragments arranged by size:
3226 543 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1213 3966
Size: 111 1102 2753
Fragments arranged by size:
2753 1102 111
BsaAI yAC'GTr
Cuts at: 0 1455 3966
Size: 1455 2511
BsaWI w'CCGG_w
Cuts at: 0 378 3966
Size: 378 3588
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1934 1964 2998 3028 3966
Size: 1934 30 1034 30 938
Fragments arranged by size:
1934 1034 938 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 215 433 559 1438 1441 1955 2284 3187
Size: 215 218 126 879 3 514 329 903
Cuts at: 3187 3190 3799 3966
Size: 3 609 167
Fragments arranged by size:
903 879 609 514 329 218 215 167
126 3 3
BseYI C'CCAG_C
Cuts at: 0 258 826 2616 2878 3966
Size: 258 568 1790 262 1088
Fragments arranged by size:
1790 1088 568 262 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1187 1306 3025 3051 3817 3865 3966
Size: 374 813 119 1719 26 766 48 101
Fragments arranged by size:
1719 813 766 374 119 101 48 26
BsiHKAI G_wGCw'C
Cuts at: 0 1775 2625 3232 3399 3637 3966
Size: 1775 850 607 167 238 329
Fragments arranged by size:
1775 850 607 329 238 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2613 3966
Size: 2613 1353
Bsp1286I G_dGCh'C
Cuts at: 0 1328 1775 2561 2625 3232 3399 3637 3795
Size: 1328 447 786 64 607 167 238 158
Cuts at: 3795 3966
Size: 171
Fragments arranged by size:
1328 786 607 447 238 171 167 158
64
BspHI T'CATG_A
Cuts at: 0 789 3966
Size: 789 3177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3526 3966
Size: 3526 440
BsrBI CCG'CTC
Cuts at: 0 402 3714 3966
Size: 402 3312 252
Fragments arranged by size:
3312 402 252
BsrDI GCAATG_nn'
Cuts at: 0 1016 2417 2730 3966
Size: 1016 1401 313 1236
Fragments arranged by size:
1401 1236 1016 313
BsrFI r'CCGG_y
Cuts at: 0 378 1535 1775 2301 3966
Size: 378 1157 240 526 1665
Fragments arranged by size:
1665 1157 526 378 240
BsrGI T'GTAC_A
Cuts at: 0 1013 1784 3966
Size: 1013 771 2182
Fragments arranged by size:
2182 1013 771
BssSI C'ACGA_G
Cuts at: 0 2278 3209 3966
Size: 2278 931 757
Fragments arranged by size:
2278 931 757
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1371 1509 3702 3966
Size: 1371 138 2193 264
Fragments arranged by size:
2193 1371 264 138
BstYI r'GATC_y
Cuts at: 0 876 900 2097 2531 3966
Size: 876 24 1197 434 1435
Fragments arranged by size:
1435 1197 876 434 24
BstZ17I GTA'TAC
Cuts at: 0 1660 3966
Size: 1660 2306
Bsu36I CC'TnA_GG
Cuts at: 0 3542 3966
Size: 3542 424
BtgI C'CryG_G
Cuts at: 0 2180 3701 3810 3859 3966
Size: 2180 1521 109 49 107
Fragments arranged by size:
2180 1521 109 107 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2174 2345 3966
Size: 2174 171 1621
Fragments arranged by size:
2174 1621 171
BtsI GCAGTG_nn'
Cuts at: 0 288 386 2079 3601 3684 3690 3966
Size: 288 98 1693 1522 83 6 276
Fragments arranged by size:
1693 1522 288 276 98 83 6
ClaI AT'CG_AT
Cuts at: 0 410 436 3966
Size: 410 26 3530
Fragments arranged by size:
3530 410 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1662 1697 3966
Size: 1662 35 2269
Fragments arranged by size:
2269 1662 35
DraIII CAC_nnn'GTG
Cuts at: 0 319 1760 3966
Size: 319 1441 2206
Fragments arranged by size:
2206 1441 319
EaeI y'GGCC_r
Cuts at: 0 64 561 998 1574 2303 2414 2765 2810
Size: 64 497 437 576 729 111 351 45
Cuts at: 2810 3092 3284 3584 3966
Size: 282 192 300 382
Fragments arranged by size:
729 576 497 437 382 351 300 282
192 111 64 45
EarI CTCTTCn'nnn_
Cuts at: 0 2161 2453 2742 2837 3651 3966
Size: 2161 292 289 95 814 315
Fragments arranged by size:
2161 814 315 292 289 95
EcoICRI GAG'CTC
Cuts at: 0 2623 3397 3635 3966
Size: 2623 774 238 331
Fragments arranged by size:
2623 774 331 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 350 766 1478 1936 2065 2090 2348
Size: 41 309 416 712 458 129 25 258
Cuts at: 2348 3262 3311 3458 3632 3764 3966
Size: 914 49 147 174 132 202
Fragments arranged by size:
914 712 458 416 309 258 202 174
147 132 129 49 41 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2366 3966
Size: 2366 1600
EcoO109I rG'GnC_Cy
Cuts at: 0 249 1325 1609 1845 2032 2091 2274 3966
Size: 249 1076 284 236 187 59 183 1692
Fragments arranged by size:
1692 1076 284 249 236 187 183 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 798 3966
Size: 798 3168
HaeII r_GCGC'y
Cuts at: 0 1302 2570 3966
Size: 1302 1268 1396
Fragments arranged by size:
1396 1302 1268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 389 421 792 824 831 863 2335 2367
Size: 389 32 371 32 7 32 1472 32
Cuts at: 2367 2620 2652 2695 2727 2998 3030 3493 3525
Size: 253 32 43 32 271 32 463 32
Cuts at: 3525 3966
Size: 441
Fragments arranged by size:
1472 463 441 389 371 271 253 43
32 32 32 32 32 32 32 32
7
HincII GTy'rAC
Cuts at: 0 1401 3220 3966
Size: 1401 1819 746
Fragments arranged by size:
1819 1401 746
HindIII A'AGCT_T
Cuts at: 0 31 3535 3966
Size: 31 3504 431
Fragments arranged by size:
3504 431 31
KpnI G_GTAC'C
Cuts at: 0 1183 3966
Size: 1183 2783
MfeI C'AATT_G
Cuts at: 0 881 3966
Size: 881 3085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 3966
Size: 551 3415
MscI TGG'CCA
Cuts at: 0 1576 2416 2767 2812 3286 3586 3966
Size: 1576 840 351 45 474 300 380
Fragments arranged by size:
1576 840 474 380 351 300 45
MslI CAynn'nnrTG
Cuts at: 0 2986 3700 3966
Size: 2986 714 266
Fragments arranged by size:
2986 714 266
MspA1I CmG'CkG
Cuts at: 0 1827 1881 2182 2878 3966
Size: 1827 54 301 696 1088
Fragments arranged by size:
1827 1088 696 301 54
NaeI GCC'GGC
Cuts at: 0 2303 3966
Size: 2303 1663
NgoMIV G'CCGG_C
Cuts at: 0 2301 3966
Size: 2301 1665
NspI r_CATG'y
Cuts at: 0 2805 3966
Size: 2805 1161
PasI CC'CwG_GG
Cuts at: 0 3726 3796 3966
Size: 3726 70 170
Fragments arranged by size:
3726 170 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 2005 2130 3966
Size: 2005 125 1836
Fragments arranged by size:
2005 1836 125
PfoI T'CCnGG_A
Cuts at: 0 1552 3966
Size: 1552 2414
PmlI CAC'GTG
Cuts at: 0 1455 3966
Size: 1455 2511
PpuMI rG'GwC_Cy
Cuts at: 0 249 1609 2032 2274 3966
Size: 249 1360 423 242 1692
Fragments arranged by size:
1692 1360 423 249 242
PspOMI G'GGCC_C
Cuts at: 0 1324 3966
Size: 1324 2642
PstI C_TGCA'G
Cuts at: 0 667 3632 3966
Size: 667 2965 334
Fragments arranged by size:
2965 667 334
PvuII CAG'CTG
Cuts at: 0 1827 3966
Size: 1827 2139
SacI G_AGCT'C
Cuts at: 0 2625 3399 3637 3966
Size: 2625 774 238 329
Fragments arranged by size:
2625 774 329 238
SacII CC_GC'GG
Cuts at: 0 2183 3966
Size: 2183 1783
SanDI GG'GwC_CC
Cuts at: 0 249 3966
Size: 249 3717
SapI GCTCTTCn'nnn_
Cuts at: 0 2161 2837 3651 3966
Size: 2161 676 814 315
Fragments arranged by size:
2161 814 676 315
SexAI A'CCwGG_T
Cuts at: 0 315 3966
Size: 315 3651
SfcI C'TryA_G
Cuts at: 0 663 1800 1863 3628 3966
Size: 663 1137 63 1765 338
Fragments arranged by size:
1765 1137 663 338 63
SmaI CCC'GGG
Cuts at: 0 347 1839 3966
Size: 347 1492 2127
Fragments arranged by size:
2127 1492 347
SmlI C'TyrA_G
Cuts at: 0 90 1228 3966
Size: 90 1138 2738
Fragments arranged by size:
2738 1138 90
StyI C'CwwG_G
Cuts at: 0 1214 1638 1976 2004 3639 3966
Size: 1214 424 338 28 1635 327
Fragments arranged by size:
1635 1214 424 338 327 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 2866 3006 3851 3966
Size: 408 2458 140 845 115
Fragments arranged by size:
2458 845 408 140 115
TatI w'GTAC_w
Cuts at: 0 1013 1784 3966
Size: 1013 771 2182
Fragments arranged by size:
2182 1013 771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1746 1778 1934 1966 3966
Size: 158 32 1556 32 156 32 2000
Fragments arranged by size:
2000 1556 158 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 51 3012 3431 3966
Size: 51 2961 419 535
Fragments arranged by size:
2961 535 419 51
XbaI T'CTAG_A
Cuts at: 0 2625 3966
Size: 2625 1341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1365 3966
Size: 1365 2601
XmaI C'CCGG_G
Cuts at: 0 345 1837 3966
Size: 345 1492 2129
Fragments arranged by size:
2129 1492 345
XmnI GAAnn'nnTTC
Cuts at: 0 2170 3966
Size: 2170 1796
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AgeI AleI AlfI
AlwNI ApaI ApoI AseI AvaI BanI BanII BbsI
BcgI BciVI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SexAI SfcI SmaI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AhdI AloI ApaLI AscI
AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI
BglI BglII BmrI BmtI BsaI BsaBI BsaHI BsiEI
BsiWI BsmI BspEI BssHII BstAPI BstBI BstEII DraI
DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI
HpaI KasI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI XhoI ZraI