Restriction map for pF1KSDA0315
 (Linear) (Six-base) MAPSORT of: KIAA0315.seq  Check: 2231  from: 1  to: 5514



KIAA0315.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      37     186     841    1112    2348    4962    5514 
   Size:         37     149     655     271    1236    2614     552

  Fragments arranged by size: 

                2614    1236     655     552     271     149      37
 
AatII G_ACGT'C

Cuts at:      0    2630    4597    5514 
   Size:       2630    1967     917

  Fragments arranged by size: 

                2630    1967     917
 
AccI GT'mk_AC

Cuts at:      0    3650    4043    5514 
   Size:       3650     393    1471

  Fragments arranged by size: 

                3650    1471     393
 
AclI AA'CG_TT

Cuts at:      0    2550    5514 
   Size:       2550    2964
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     873    3006    5514 
   Size:        873    2133    2508

  Fragments arranged by size: 

                2508    2133     873
 
AfeI AGC'GCT

Cuts at:      0    3406    5514 
   Size:       3406    2108
 
AflIII A'CryG_T

Cuts at:      0    3134    5514 
   Size:       3134    2380
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4593    5514 
   Size:       4593     921
 
AleI CACnn'nnGTG

Cuts at:      0     744    1147    1180    3574    5362    5514 
   Size:        744     403      33    2394    1788     152

  Fragments arranged by size: 

                2394    1788     744     403     152      33
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     762    1165    2131    3835    5230    5495    5514 
   Size:        762     403     966    1704    1395     265      19

  Fragments arranged by size: 

                1704    1395     966     762     403     265      19
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    5514 
   Size:       1366      32    4116

  Fragments arranged by size: 

                4116    1366      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     297    1526    2007    2297    4575    4719    5514 
   Size:        297    1229     481     290    2278     144     795

  Fragments arranged by size: 

                2278    1229     795     481     297     290     144
 
ApaI G_GGCC'C

Cuts at:      0      21     211     920    1599    2440    4306    5514 
   Size:         21     190     709     679     841    1866    1208

  Fragments arranged by size: 

                1866    1208     841     709     679     190      21
 
ApaLI G'TGCA_C

Cuts at:      0     231     955    3799    5514 
   Size:        231     724    2844    1715

  Fragments arranged by size: 

                2844    1715     724     231
 
ApoI r'AATT_y

Cuts at:      0    3754    5514 
   Size:       3754    1760
 
AvaI C'yCGr_G

Cuts at:      0    1001    3341    3511    5333    5514 
   Size:       1001    2340     170    1822     181

  Fragments arranged by size: 

                2340    1822    1001     181     170
 
BamHI G'GATC_C

Cuts at:      0    1241    2420    5273    5433    5514 
   Size:       1241    1179    2853     160      81

  Fragments arranged by size: 

                2853    1241    1179     160      81
 
BanI G'GyrC_C

Cuts at:      0      49     829     997    1225    1270    2728    2749    3230 
   Size:         49     780     168     228      45    1458      21     481

Cuts at:   3230    3346    3397    4766    4932    4947    5514 
   Size:        116      51    1369     166      15     567

  Fragments arranged by size: 

                1458    1369     780     567     481     228     168     166
                 116      51      49      45      21      15
 
BanII G_rGCy'C

Cuts at:      0      21     211     475     920    1072    1136    1599    2440 
   Size:         21     190     264     445     152      64     463     841

Cuts at:   2440    2770    3065    3452    4127    4259    4306    4561    5514 
   Size:        330     295     387     675     132      47     255     953

  Fragments arranged by size: 

                 953     841     675     463     445     387     330     295
                 264     255     190     152     132      64      47      21
            
 
BbeI G_GCGC'C

Cuts at:      0    4936    5514 
   Size:       4936     578
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     675     684     761     879    2622    3153    3424    3843 
   Size:        675       9      77     118    1743     531     271     419

Cuts at:   3843    5024    5044    5514 
   Size:       1181      20     470

  Fragments arranged by size: 

                1743    1181     675     531     470     419     271     118
                  77      20       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2226    2260    3354    3388    5514 
   Size:       2226      34    1094      34    2126

  Fragments arranged by size: 

                2226    2126    1094      34      34
 
BclI T'GATC_A

Cuts at:      0    3890    5514 
   Size:       3890    1624
 
BglI GCCn_nnn'nGGC

Cuts at:      0      41     850    1516    3477    3924    5514 
   Size:         41     809     666    1961     447    1590

  Fragments arranged by size: 

                1961    1590     809     666     447      41
 
BglII A'GATC_T

Cuts at:      0    4856    5231    5514 
   Size:       4856     375     283

  Fragments arranged by size: 

                4856     375     283
 
BlpI GC'TnA_GC

Cuts at:      0    3592    5188    5514 
   Size:       3592    1596     326

  Fragments arranged by size: 

                3592    1596     326
 
Bme1580I G_kGCm'C

Cuts at:      0      21     211     235     920     959    1228    1599    1980 
   Size:         21     190      24     685      39     269     371     381

Cuts at:   1980    2401    2440    2912    3233    3240    3349    3623    3803 
   Size:        421      39     472     321       7     109     274     180

Cuts at:   3803    4306    4891    4943    5514 
   Size:        503     585      52     571

  Fragments arranged by size: 

                 685     585     571     503     472     421     381     371
                 321     274     269     190     180     109      52      39
                  39      24      21       7
 
BmgBI CAC'GTC

Cuts at:      0    1817    2781    3585    3798    3885    5103    5514 
   Size:       1817     964     804     213      87    1218     411

  Fragments arranged by size: 

                1817    1218     964     804     411     213      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1422    1995    2650    5514 
   Size:       1422     573     655    2864

  Fragments arranged by size: 

                2864    1422     655     573
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1305    4681    5514 
   Size:       1305    3376     833

  Fragments arranged by size: 

                3376    1305     833
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     799    1056    1361    1882    2821    2902    3681 
   Size:        208     591     257     305     521     939      81     779

Cuts at:   3681    3733    3742    4027    4105    4141    4150    4564    4765 
   Size:         52       9     285      78      36       9     414     201

Cuts at:   4765    5512    5514 
   Size:        747       2

  Fragments arranged by size: 

                 939     779     747     591     521     414     305     285
                 257     208     201      81      78      52      36       9
                   9       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     373    1408    1639    3914    5514 
   Size:        373    1035     231    2275    1600

  Fragments arranged by size: 

                2275    1600    1035     373     231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1231    2104    4222    5062    5514 
   Size:       1231     873    2118     840     452

  Fragments arranged by size: 

                2118    1231     873     840     452
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1337    2892    3424    4692    5514 
   Size:       1337    1555     532    1268     822

  Fragments arranged by size: 

                1555    1337    1268     822     532
 
BsaAI yAC'GTr

Cuts at:      0    1767    2103    3135    3273    4140    5514 
   Size:       1767     336    1032     138     867    1374

  Fragments arranged by size: 

                1767    1374    1032     867     336     138
 
BsaBI GATnn'nnATC

Cuts at:      0    4216    5514 
   Size:       4216    1298
 
BsaHI Gr'CG_yC

Cuts at:      0    2486    2599    2627    2801    3308    4424    4551    4594 
   Size:       2486     113      28     174     507    1116     127      43

Cuts at:   4594    4933    5126    5514 
   Size:        339     193     388

  Fragments arranged by size: 

                2486    1116     507     388     339     193     174     127
                 113      43      28
 
BsaWI w'CCGG_w

Cuts at:      0     566    5057    5514 
   Size:        566    4491     457

  Fragments arranged by size: 

                4491     566     457
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     754     784    1639    1669    2263    2293    2374    2404 
   Size:        754      30     855      30     594      30      81      30

Cuts at:   2404    2752    2782    3940    3970    4246    4276    4444    4474 
   Size:        348      30    1158      30     276      30     168      30

Cuts at:   4474    4597    4627    5332    5362    5514 
   Size:        123      30     705      30     152

  Fragments arranged by size: 

                1158     855     754     705     594     348     276     168
                 152     123      81      30      30      30      30      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1266    1528    1660    1777    1879    3199    3739    4762 
   Size:       1266     262     132     117     102    1320     540    1023

Cuts at:   4762    4765    5443    5514 
   Size:          3     678      71

  Fragments arranged by size: 

                1320    1266    1023     678     540     262     132     117
                 102      71       3
 
BseYI C'CCAG_C

Cuts at:      0      30      39    1116    1571    2014    3549    3707    4359 
   Size:         30       9    1077     455     443    1535     158     652

Cuts at:   4359    4691    5203    5514 
   Size:        332     512     311

  Fragments arranged by size: 

                1535    1077     652     512     455     443     332     311
                 158      30       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     768    1407    1417    1630    2083    2197    3393    3481 
   Size:        768     639      10     213     453     114    1196      88

Cuts at:   3481    4057    4426    5151    5308    5471    5514 
   Size:        576     369     725     157     163      43

  Fragments arranged by size: 

                1196     768     725     639     576     453     369     213
                 163     157     114      88      43      10
 
BsiEI CG_ry'CG

Cuts at:      0    1181    1190    2526    3846    3928    4780    4784    5514 
   Size:       1181       9    1336    1320      82     852       4     730

  Fragments arranged by size: 

                1336    1320    1181     852     730      82       9       4
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     235     475     553     959    1103    3803    4127    5514 
   Size:        235     240      78     406     144    2700     324    1387

  Fragments arranged by size: 

                2700    1387     406     324     240     235     144      78
            
 
BsmI GAATG_Cn'

Cuts at:      0     159    5514 
   Size:        159    5355
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2194    2425    2590    5514 
   Size:       2194     231     165    2924

  Fragments arranged by size: 

                2924    2194     231     165
 
Bsp1286I G_dGCh'C

Cuts at:      0      21     211     235     475     553     920     959    1072 
   Size:         21     190      24     240      78     367      39     113

Cuts at:   1072    1103    1136    1228    1599    1980    2401    2440    2770 
   Size:         31      33      92     371     381     421      39     330

Cuts at:   2770    2912    3065    3233    3240    3349    3452    3623    3803 
   Size:        142     153     168       7     109     103     171     180

Cuts at:   3803    4127    4259    4306    4561    4891    4943    5514 
   Size:        324     132      47     255     330      52     571

  Fragments arranged by size: 

                 571     421     381     371     367     330     330     324
                 255     240     190     180     171     168     153     142
                 132     113     109     103      92      78      52      47
                  39      39      33      31      24      21       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      37     186     797     841    1112    1429    1880    1915 
   Size:         37     149     611      44     271     317     451      35

Cuts at:   1915    1928    2348    3309    3515    3971    4163    4727    4962 
   Size:         13     420     961     206     456     192     564     235

Cuts at:   4962    5280    5514 
   Size:        318     234

  Fragments arranged by size: 

                 961     611     564     456     451     420     318     317
                 271     235     234     206     192     149      44      37
                  35      13
 
BsrBI CCG'CTC

Cuts at:      0    3591    4836    5055    5514 
   Size:       3591    1245     219     459

  Fragments arranged by size: 

                3591    1245     459     219
 
BsrDI GCAATG_nn'

Cuts at:      0     444     525    5514 
   Size:        444      81    4989

  Fragments arranged by size: 

                4989     444      81
 
BsrFI r'CCGG_y

Cuts at:      0     622    1392    1589    2133    2521    3808    3895    3927 
   Size:        622     770     197     544     388    1287      87      32

Cuts at:   3927    4610    5514 
   Size:        683     904

  Fragments arranged by size: 

                1287     904     770     683     622     544     388     197
                  87      32
 
BsrGI T'GTAC_A

Cuts at:      0    4262    5514 
   Size:       4262    1252
 
BssHII G'CGCG_C

Cuts at:      0     366    5514 
   Size:        366    5148
 
BssSI C'ACGA_G

Cuts at:      0    1930    2041    3802    5113    5362    5514 
   Size:       1930     111    1761    1311     249     152

  Fragments arranged by size: 

                1930    1761    1311     249     152     111
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1430    1550    1886    2297    2306    4719    4773    4947 
   Size:       1430     120     336     411       9    2413      54     174

Cuts at:   4947    5286    5514 
   Size:        339     228

  Fragments arranged by size: 

                2413    1430     411     339     336     228     174     120
                  54       9
 
BstEII G'GTnAC_C

Cuts at:      0     490    2136    2787    5514 
   Size:        490    1646     651    2727

  Fragments arranged by size: 

                2727    1646     651     490
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1860    2022    3117    5514 
   Size:       1860     162    1095    2397

  Fragments arranged by size: 

                2397    1860    1095     162
 
BstYI r'GATC_y

Cuts at:      0    1241    2420    2510    4571    4856    5231    5273    5433 
   Size:       1241    1179      90    2061     285     375      42     160

Cuts at:   5433    5514 
   Size:         81

  Fragments arranged by size: 

                2061    1241    1179     375     285     160      90      81
                  42
 
Bsu36I CC'TnA_GG

Cuts at:      0      58     126    1958    3182    5514 
   Size:         58      68    1832    1224    2332

  Fragments arranged by size: 

                2332    1832    1224      68      58
 
BtgI C'CryG_G

Cuts at:      0     203     476     848    1035    1504    2745    3044    3085 
   Size:        203     273     372     187     469    1241     299      41

Cuts at:   3085    3623    3919    4497    5514 
   Size:        538     296     578    1017

  Fragments arranged by size: 

                1241    1017     578     538     469     372     299     296
                 273     203     187      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3039    3379    3621    5127    5514 
   Size:       3039     340     242    1506     387

  Fragments arranged by size: 

                3039    1506     387     340     242
 
BtsI GCAGTG_nn'

Cuts at:      0       4     799     915    2115    4891    5514 
   Size:          4     795     116    1200    2776     623

  Fragments arranged by size: 

                2776    1200     795     623     116       4
 
DraIII CAC_nnn'GTG

Cuts at:      0    1430    4773    4947    5118    5514 
   Size:       1430    3343     174     171     396

  Fragments arranged by size: 

                3343    1430     396     174     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1969    3154    4537    5107    5514 
   Size:       1969    1185    1383     570     407

  Fragments arranged by size: 

                1969    1383    1185     570     407
 
EaeI y'GGCC_r

Cuts at:      0     200     431     452     839    1056    1178    1236    1556 
   Size:        200     231      21     387     217     122      58     320

Cuts at:   1556    1769    2286    2519    2523    2991    3925    4145    4777 
   Size:        213     517     233       4     468     934     220     632

Cuts at:   4777    5514 
   Size:        737

  Fragments arranged by size: 

                 934     737     632     517     468     387     320     233
                 231     220     217     213     200     122      58      21
                   4
 
EagI C'GGCC_G

Cuts at:      0    1178    2523    3925    4777    5514 
   Size:       1178    1345    1402     852     737

  Fragments arranged by size: 

                1402    1345    1178     852     737
 
EarI CTCTTCn'nnn_

Cuts at:      0     349    3661    4120    4276    5514 
   Size:        349    3312     459     156    1238

  Fragments arranged by size: 

                3312    1238     459     349     156
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     379    5455    5514 
   Size:        379    5076      59

  Fragments arranged by size: 

                5076     379      59
 
EcoICRI GAG'CTC

Cuts at:      0     473    4125    5514 
   Size:        473    3652    1389

  Fragments arranged by size: 

                3652    1389     473
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     799     873    1056    1361    1882    2821    2902 
   Size:        208     591      74     183     305     521     939      81

Cuts at:   2902    3006    3681    3733    3742    4027    4105    4141    4150 
   Size:        104     675      52       9     285      78      36       9

Cuts at:   4150    4564    4765    5512    5514 
   Size:        414     201     747       2

  Fragments arranged by size: 

                 939     747     675     591     521     414     305     285
                 208     201     183     104      81      78      74      52
                  36       9       9       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     346    1642    4675    4744    4747    5514 
   Size:        346    1296    3033      69       3     767

  Fragments arranged by size: 

                3033    1296     767     346      69       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18      62     481     801     916    1446    1917    2011 
   Size:         18      44     419     320     115     530     471      94

Cuts at:   2011    2156    2373    2437    2900    3260    3717    3942    4302 
   Size:        145     217      64     463     360     457     225     360

Cuts at:   4302    4440    4716    5514 
   Size:        138     276     798

  Fragments arranged by size: 

                 798     530     471     463     457     419     360     360
                 320     276     225     217     145     138     115      94
                  64      44      18
 
EcoRI G'AATT_C

Cuts at:      0    3754    5514 
   Size:       3754    1760
 
EcoRV GAT'ATC

Cuts at:      0    1044    5514 
   Size:       1044    4470
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      93    3177    4062    4986    5514 
   Size:         93    3084     885     924     528

  Fragments arranged by size: 

                3084     924     885     528      93
 
FseI GG_CCGG'CC

Cuts at:      0    2525    5514 
   Size:       2525    2989
 
FspI TGC'GCA

Cuts at:      0    4171    5514 
   Size:       4171    1343
 
HaeII r_GCGC'y

Cuts at:      0     324    1571    3408    4072    4734    4936    5514 
   Size:        324    1247    1837     664     662     202     578

  Fragments arranged by size: 

                1837    1247     664     662     578     324     202
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141    1258    1290    1790    1822    2752    2784 
   Size:        109      32    1117      32     500      32     930      32

Cuts at:   2784    3082    3114    4444    4476    4579    4611    4789    4821 
   Size:        298      32    1330      32     103      32     178      32

Cuts at:   4821    5514 
   Size:        693

  Fragments arranged by size: 

                1330    1117     930     693     500     298     178     109
                 103      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     288    3324    4626    5514 
   Size:        288    3036    1302     888

  Fragments arranged by size: 

                3036    1302     888     288
 
HindIII A'AGCT_T

Cuts at:      0    2976    5514 
   Size:       2976    2538
 
KasI G'GCGC_C

Cuts at:      0    4932    5514 
   Size:       4932     582
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    2700    3983    4229    4559    5514 
   Size:        542    2158    1283     246     330     955

  Fragments arranged by size: 

                2158    1283     955     542     330     246
 
MscI TGG'CCA

Cuts at:      0     202     433     454    4147    5514 
   Size:        202     231      21    3693    1367

  Fragments arranged by size: 

                3693    1367     231     202      21
 
MslI CAynn'nnrTG

Cuts at:      0     544     744    1147    1180    3115    3574    3640    3817 
   Size:        544     200     403      33    1935     459      66     177

Cuts at:   3817    4432    4633    4643    5362    5514 
   Size:        615     201      10     719     152

  Fragments arranged by size: 

                1935     719     615     544     459     403     201     200
                 177     152      66      33      10
 
MspA1I CmG'CkG

Cuts at:      0     168     186     294     413     820     945    1116    1506 
   Size:        168      18     108     119     407     125     171     390

Cuts at:   1506    1614    1901    1994    3087    3609    3711    3743    3921 
   Size:        108     287      93    1093     522     102      32     178

Cuts at:   3921    4017    4602    5457    5485    5514 
   Size:         96     585     855      28      29

  Fragments arranged by size: 

                1093     855     585     522     407     390     287     178
                 171     168     125     119     108     108     102      96
                  93      32      29      28      18
 
NaeI GCC'GGC

Cuts at:      0     624    1591    2523    3810    5514 
   Size:        624     967     932    1287    1704

  Fragments arranged by size: 

                1704    1287     967     932     624
 
NarI GG'CG_CC

Cuts at:      0    4933    5514 
   Size:       4933     581
 
NcoI C'CATG_G

Cuts at:      0    3044    3623    5514 
   Size:       3044     579    1891

  Fragments arranged by size: 

                3044    1891     579
 
NgoMIV G'CCGG_C

Cuts at:      0     622    1589    2521    3808    5514 
   Size:        622     967     932    1287    1706

  Fragments arranged by size: 

                1706    1287     967     932     622
 
NruI TCG'CGA

Cuts at:      0    3539    5514 
   Size:       3539    1975
 
NspI r_CATG'y

Cuts at:      0     747    5110    5514 
   Size:        747    4363     404

  Fragments arranged by size: 

                4363     747     404
 
PasI CC'CwG_GG

Cuts at:      0    1599    2442    5514 
   Size:       1599     843    3072

  Fragments arranged by size: 

                3072    1599     843
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1526    2007    4005    5118    5514 
   Size:       1526     481    1998    1113     396

  Fragments arranged by size: 

                1998    1526    1113     481     396
 
PfoI T'CCnGG_A

Cuts at:      0    4118    4127    4541    4649    4742    4979    5009    5514 
   Size:       4118       9     414     108      93     237      30     505

  Fragments arranged by size: 

                4118     505     414     237     108      93      30       9
            
 
PmlI CAC'GTG

Cuts at:      0    1767    2103    5514 
   Size:       1767     336    3411

  Fragments arranged by size: 

                3411    1767     336
 
PpuMI rG'GwC_Cy

Cuts at:      0     481     801    1446    1917    2011    2156    2900    4440 
   Size:        481     320     645     471      94     145     744    1540

Cuts at:   4440    4716    5514 
   Size:        276     798

  Fragments arranged by size: 

                1540     798     744     645     481     471     320     276
                 145      94
 
PshAI GACnn'nnGTC

Cuts at:      0    1994    5514 
   Size:       1994    3520
 
PspOMI G'GGCC_C

Cuts at:      0      17     207     916    1595    2436    4302    5514 
   Size:         17     190     709     679     841    1866    1212

  Fragments arranged by size: 

                1866    1212     841     709     679     190      17
 
PstI C_TGCA'G

Cuts at:      0      11     185     794    1157    2302    3071    3107    3377 
   Size:         11     174     609     363    1145     769      36     270

Cuts at:   3377    4898    4959    5474    5514 
   Size:       1521      61     515      40

  Fragments arranged by size: 

                1521    1145     769     609     515     363     270     174
                  61      40      36      11
 
PvuII CAG'CTG

Cuts at:      0     168     186     294    1614    1994    3711    5457    5514 
   Size:        168      18     108    1320     380    1717    1746      57

  Fragments arranged by size: 

                1746    1717    1320     380     168     108      57      18
            
 
SacI G_AGCT'C

Cuts at:      0     475    4127    5514 
   Size:        475    3652    1387

  Fragments arranged by size: 

                3652    1387     475
 
SacII CC_GC'GG

Cuts at:      0    1507    3088    3922    5514 
   Size:       1507    1581     834    1592

  Fragments arranged by size: 

                1592    1581    1507     834
 
SanDI GG'GwC_CC

Cuts at:      0     801    2011    2900    5514 
   Size:        801    1210     889    2614

  Fragments arranged by size: 

                2614    1210     889     801
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3661    4120    4276    5514 
   Size:       3661     459     156    1238

  Fragments arranged by size: 

                3661    1238     459     156
 
ScaI AGT'ACT

Cuts at:      0    4090    4966    5401    5514 
   Size:       4090     876     435     113

  Fragments arranged by size: 

                4090     876     435     113
 
SexAI A'CCwGG_T

Cuts at:      0    1328    4487    4769    5514 
   Size:       1328    3159     282     745

  Fragments arranged by size: 

                3159    1328     745     282
 
SfcI C'TryA_G

Cuts at:      0       7     181     790    1153    2298    2328    3067    3103 
   Size:          7     174     609     363    1145      30     739      36

Cuts at:   3103    3373    4894    4955    5470    5514 
   Size:        270    1521      61     515      44

  Fragments arranged by size: 

                1521    1145     739     609     515     363     270     174
                  61      44      36      30       7
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1516    5514 
   Size:       1516    3998
 
SfoI GGC'GCC

Cuts at:      0    4934    5514 
   Size:       4934     580
 
SmaI CCC'GGG

Cuts at:      0    1003    3343    5335    5514 
   Size:       1003    2340    1992     179

  Fragments arranged by size: 

                2340    1992    1003     179
 
SmlI C'TyrA_G

Cuts at:      0    1246    2119    4237    5077    5514 
   Size:       1246     873    2118     840     437

  Fragments arranged by size: 

                2118    1246     873     840     437
 
StuI AGG'CCT

Cuts at:      0     124     586    1166    3196    3388    5514 
   Size:        124     462     580    2030     192    2126

  Fragments arranged by size: 

                2126    2030     580     462     192     124
 
StyI C'CwwG_G

Cuts at:      0    3044    3152    3623    3866    4037    4676    5225    5423 
   Size:       3044     108     471     243     171     639     549     198

Cuts at:   5423    5514 
   Size:         91

  Fragments arranged by size: 

                3044     639     549     471     243     198     171     108
                  91
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1441    2434    2767    3385    3448    3534    4798    5055 
   Size:       1441     993     333     618      63      86    1264     257

Cuts at:   5055    5514 
   Size:        459

  Fragments arranged by size: 

                1441    1264     993     618     459     333     257      86
                  63
 
TatI w'GTAC_w

Cuts at:      0    3440    4088    4262    4334    4964    5399    5514 
   Size:       3440     648     174      72     630     435     115

  Fragments arranged by size: 

                3440     648     630     435     174     115      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     778     810    2562    2594    2726    2758    4597    4629 
   Size:        778      32    1752      32     132      32    1839      32

Cuts at:   4629    5332    5364    5514 
   Size:        703      32     150

  Fragments arranged by size: 

                1839    1752     778     703     150     132      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     283    3847    5514 
   Size:        283    3564    1667

  Fragments arranged by size: 

                3564    1667     283
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     321     462    2845    3117    4495    5514 
   Size:        321     141    2383     272    1378    1019

  Fragments arranged by size: 

                2383    1378    1019     321     272     141
 
XmaI C'CCGG_G

Cuts at:      0    1001    3341    5333    5514 
   Size:       1001    2340    1992     181

  Fragments arranged by size: 

                2340    1992    1001     181
 
XmnI GAAnn'nnTTC

Cuts at:      0     424    3754    5514 
   Size:        424    3330    1760

  Fragments arranged by size: 

                3330    1760     424
 
ZraI GAC'GTC

Cuts at:      0    2628    4595    5514 
   Size:       2628    1967     919

  Fragments arranged by size: 

                2628    1967     919
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I    AflII     AgeI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI    BciVI    BfrBI     BmtI    BsiWI    BspEI    BspHI    BstBI
  BstZ17I     ClaI    CspCI     DraI    FspAI     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NsiI     PacI     PciI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SbfI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI
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