Restriction map for pF1KSDA0276
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MfeI        
MluI        MroI        MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          239  GTAGAC               GT^[AC][GT]AC
AcsI          379  AAATTT               [GA]^AATT[CT]
AflII         546  CTTAAG               C^TTAAG
AlwNI         341  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          379  AAATTT               [GA]^AATT[CT]
Asp700I       491  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         621  GGACC                G^G[AT]CC
BbvCI         795  CCTCAGC              CCTCAGC(-5/-2)
BbvI          629  GCAGC                GCAGC(8/12)
BfrI          546  CTTAAG               C^TTAAG
BglI          362  GCCCTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          71  AGATCT               A^GATCT
BpmI          212  CTGGAG               CTGGAG(16/14)
BsaJI         187  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         205  CCTGCCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          205  CCTGCCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         663  GGGAC                GGGAC(10/14)
BspMI         204  ACCTGC               ACCTGC(4/8)
BsrDI         758  GCAATG               GCAATG(2/0)
BstYI          71  AGATCT               [GA]^GATC[CT]
CfoI          627  GCGC                 GCG^C
DraI          577  TTTAAA               TTT^AAA
DraII         360  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      360  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       187  CCAAGG               C^C[AT][AT]GG
HhaI          627  GCGC                 GCG^C
HinP1I        627  GCGC                 G^CGC
MaeII         413  ACGT                 A^CGT
MflI           71  AGATCT               [GA]^GATC[CT]
MslI            4  CACTCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          362  GCCCTGAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         686  GGCCCC               GG[ACGT]^[ACGT]CC
PvuII          31  CAGCTG               CAG^CTG
SfcI          472  CTACAG               C^T[GA][CT]AG
SmlI          546  CTTAAG               C^T[CT][GA]AG
SspI           61  AATATT               AAT^ATT
StyI          187  CCAAGG               C^C[AT][AT]GG
TaqI          271  TCGA                 T^CGA
TseI          629  GCAGC                G^C[AT]GC
TthHB8I       271  TCGA                 T^CGA
XcmI          650  CCAAGTGCATGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          71  AGATCT               [GA]^GATC[CT]
XmnI          491  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AseI          739  ATTAAT               AT^TAAT
              784  ATTAAT              
AsnI          739  ATTAAT               AT^TAAT
              784  ATTAAT              
BalI          114  TGGCCA               TGG^CCA
              823  TGGCCA              
BstF5I          9  GGATG                GGATG(2/0)
              444  GGATG               
EaeI          114  TGGCCA               [CT]^GGCC[GA]
              823  TGGCCA              
FokI            9  GGATG                GGATG(9/13)
              444  GGATG               
HinfI         268  GATTC                G^A[ACGT]TC
              565  GATTC               
HphI          126  TCACC                GGTGA(8/7)
              705  TCACC               
MluNI         114  TGGCCA               TGG^CCA
              823  TGGCCA              
MnlI          209  CCTC                 CCTC(7/6)
              795  CCTC                
MscI          114  TGGCCA               TGG^CCA
              823  TGGCCA              
MspA1I         17  CCGCTG               C[AC]G^C[GT]G
               31  CAGCTG              
NspI          251  GCATGT               [GA]CATG^[CT]
              656  GCATGT              
PshBI         739  ATTAAT               AT^TAAT
              784  ATTAAT              
TfiI          268  GATTC                G^A[AT]TC
              565  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbsI        BfaI        BpuAI       
BsrI        Cfr13I      DdeI        DpnII       Fnu4HI      
HaeIII      ItaI        MaeI        MboI        MboII       
MseI        NdeII       NlaIII      Sau3AI      Sau96I      
SfaNI       Tru9I       Tsp509I     TspRI       
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