Restriction map for pF1KSDA0245
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       Alw44I      AocI        ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbsI        
BbvCI       BciVI       BclI        BfrI        BlnI        
BpuAI       BsaBI       BseAI       BseRI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmeI        PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AcsI          795  AAATTT               [GA]^AATT[CT]
AcyI          670  GACGCC               G[GA]^CG[CT]C
AfaI          214  GTAC                 GT^AC
AgeI          412  ACCGGT               A^CCGGT
AhdI         1060  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1185  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       603  AGCGCT               AGC^GCT
ApoI          795  AAATTT               [GA]^AATT[CT]
AseI         1345  ATTAAT               AT^TAAT
AsnI         1345  ATTAAT               AT^TAAT
AspEI        1060  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         658  GAGCAC               G[AT]GC[AT]^C
AvaI         1040  CCCGGG               C^[CT]CG[GA]G
BalI          806  TGGCCA               TGG^CCA
BbrPI         566  CACGTG               CAC^GTG
BcgI          167  GCAACATGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         131  AGATCT               A^GATCT
BlpI         1269  GCTGAGC              GC^T[ACGT]AGC
BpmI         1379  CTGGAG               CTGGAG(16/14)
Bpu1102I     1269  GCTGAGC              GC^T[ACGT]AGC
BsaAI         566  CACGTG               [CT]AC^GT[GA]
BsaHI         670  GACGCC               G[GA]^CG[CT]C
BsaI          387  GGTCTC               GGTCTC(1/5)
BsgI          571  GTGCAG               GTGCAG(16/14)
BsiHKAI       658  GAGCAC               G[AT]GC[AT]^C
BsmBI         499  CGTCTC               CGTCTC(1/5)
BsoBI        1040  CCCGGG               C^[CT]CG[GA]G
BspMI        1409  ACCTGC               ACCTGC(4/8)
BsrFI         412  ACCGGT               [GA]^CCGG[CT]
BstXI         930  CCAGACGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1269  GCTGAGC              GC^T[ACGT]AGC
CfoI          604  GCGC                 GCG^C
Cfr10I        412  ACCGGT               [GA]^CCGG[CT]
DsaI         1124  CCATGG               C^C[GA][CT]GG
EaeI          806  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1060  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      603  AGCGCT               AGC^GCT
EcoT22I       998  ATGCAT               ATGCA^T
HaeII         603  AGCGCT               [GA]GCGC^[CT]
HhaI          604  GCGC                 GCG^C
Hin1I         670  GACGCC               G[GA]^CG[CT]C
HinP1I        604  GCGC                 G^CGC
MluNI         806  TGGCCA               TGG^CCA
MscI          806  TGGCCA               TGG^CCA
NcoI         1124  CCATGG               C^CATGG
NsiI          998  ATGCAT               ATGCA^T
NspI          941  GCATGT               [GA]CATG^[CT]
PinAI         412  ACCGGT               A^CCGGT
PmaCI         566  CACGTG               CAC^GTG
PmlI          566  CACGTG               CAC^GTG
PpuMI         100  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1345  ATTAAT               AT^TAAT
RsaI          214  GTAC                 GT^AC
SapI          264  GCTCTTC              GCTCTTC(1/4)
SmaI         1040  CCCGGG               CCC^GGG
SmlI         1264  CTCAAG               C^T[CT][GA]AG
StuI          350  AGGCCT               AGG^CCT
XbaI          345  TCTAGA               T^CTAGA
XmaCI        1040  CCCGGG               C^CCGGG
XmaI         1040  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanI          280  GGTGCC               G^G[CT][GA]CC
             1468  GGCACC              
BfaI          346  CTAG                 C^TAG
              397  CTAG                
BglI           15  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1245  GCCCAAGCGGC         
BsaWI         412  ACCGGT               [AT]^CCGG[AT]
             1056  ACCGGA              
BsrI         1190  ACTGG                ACTGG(1/-1)
               60  CCAGT               
BstYI         131  AGATCT               [GA]^GATC[CT]
              185  GGATCT              
DraII         100  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              989  GGGGCCT             
EcoO109I      100  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              989  GGGGCCT             
HgaI          670  GACGC                GACGC(5/10)
              834  GACGC               
HinfI        1382  GAGTC                G^A[ACGT]TC
             1417  GAGTC               
MaeI          346  CTAG                 C^TAG
              397  CTAG                
MfeI          827  CAATTG               C^AATTG
             1116  CAATTG              
MflI          131  AGATCT               [GA]^GATC[CT]
              185  GGATCT              
MslI          564  CACACGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              618  CATTGTCATG          
MunI          827  CAATTG               C^AATTG
             1116  CAATTG              
NdeI          519  CATATG               CA^TATG
             1299  CATATG              
PleI         1382  GAGTC                GAGTC(4/5)
             1417  GAGTC               
XcmI          200  CCAAGGGTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              614  CCATCATTGTCATGG     
XhoII         131  AGATCT               [GA]^GATC[CT]
              185  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AflIII      AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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