Restriction map for
pF1KSDA0245
(Linear) (Six-base) MAPSORT of: KIAA0245.seq Check: 8352 from: 1 to: 1545
KIAA0245.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1161 1167 1545
Size: 142 1019 6 378
Fragments arranged by size:
1019 378 142 6
AfeI AGC'GCT
Cuts at: 0 605 1545
Size: 605 940
AflIII A'CryG_T
Cuts at: 0 565 567 578 1545
Size: 565 2 11 967
Fragments arranged by size:
967 565 11 2
AgeI A'CCGG_T
Cuts at: 0 412 1545
Size: 412 1133
AhdI GACnn_n'nnGTC
Cuts at: 0 1065 1545
Size: 1065 480
AleI CACnn'nnGTG
Cuts at: 0 568 1545
Size: 568 977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 586 1545
Size: 541 45 959
Fragments arranged by size:
959 541 45
AlwNI CAG_nnn'CTG
Cuts at: 0 1190 1545
Size: 1190 355
ApoI r'AATT_y
Cuts at: 0 795 1545
Size: 795 750
AseI AT'TA_AT
Cuts at: 0 1346 1545
Size: 1346 199
AvaI C'yCGr_G
Cuts at: 0 1040 1545
Size: 1040 505
BanI G'GyrC_C
Cuts at: 0 280 1468 1545
Size: 280 1188 77
Fragments arranged by size:
1188 280 77
BanII G_rGCy'C
Cuts at: 0 266 410 1039 1247 1545
Size: 266 144 629 208 298
Fragments arranged by size:
629 298 266 208 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 156 190 1545
Size: 156 34 1355
Fragments arranged by size:
1355 156 34
BfrBI ATG'CAT
Cuts at: 0 1000 1545
Size: 1000 545
BglI GCCn_nnn'nGGC
Cuts at: 0 21 1251 1545
Size: 21 1230 294
Fragments arranged by size:
1230 294 21
BglII A'GATC_T
Cuts at: 0 131 1545
Size: 131 1414
BlpI GC'TnA_GC
Cuts at: 0 1270 1545
Size: 1270 275
Bme1580I G_kGCm'C
Cuts at: 0 285 565 1328 1545
Size: 285 280 763 217
Fragments arranged by size:
763 285 280 217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 702 1545
Size: 702 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1545
Size: 1400 145
Bpu10I CC'TnA_GC
Cuts at: 0 180 895 1545
Size: 180 715 650
Fragments arranged by size:
715 650 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1545
Size: 1249 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 393 1545
Size: 393 1152
BsaAI yAC'GTr
Cuts at: 0 568 1545
Size: 568 977
BsaHI Gr'CG_yC
Cuts at: 0 671 1545
Size: 671 874
BsaWI w'CCGG_w
Cuts at: 0 412 1056 1545
Size: 412 644 489
Fragments arranged by size:
644 489 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 1545
Size: 208 30 1307
Fragments arranged by size:
1307 208 30
BseYI C'CCAG_C
Cuts at: 0 300 469 1472 1545
Size: 300 169 1003 73
Fragments arranged by size:
1003 300 169 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1545
Size: 592 953
BsiHKAI G_wGCw'C
Cuts at: 0 662 1545
Size: 662 883
BsmBI CGTCTCn'nnnn_
Cuts at: 0 505 1545
Size: 505 1040
Bsp1286I G_dGCh'C
Cuts at: 0 266 285 410 565 662 1039 1247 1328
Size: 266 19 125 155 97 377 208 81
Cuts at: 1328 1545
Size: 217
Fragments arranged by size:
377 266 217 208 155 125 97 81
19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1418 1545
Size: 1418 127
BsrDI GCAATG_nn'
Cuts at: 0 365 608 962 1545
Size: 365 243 354 583
Fragments arranged by size:
583 365 354 243
BsrFI r'CCGG_y
Cuts at: 0 412 1545
Size: 412 1133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 937 1545
Size: 937 608
BstYI r'GATC_y
Cuts at: 0 131 185 1545
Size: 131 54 1360
Fragments arranged by size:
1360 131 54
BtgI C'CryG_G
Cuts at: 0 1124 1545
Size: 1124 421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 1545
Size: 411 1134
BtsI GCAGTG_nn'
Cuts at: 0 218 391 906 1545
Size: 218 173 515 639
Fragments arranged by size:
639 515 218 173
EaeI y'GGCC_r
Cuts at: 0 806 1545
Size: 806 739
EarI CTCTTCn'nnn_
Cuts at: 0 271 736 1336 1545
Size: 271 465 600 209
Fragments arranged by size:
600 465 271 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 367 1545
Size: 367 1178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1161 1167 1400 1545
Size: 142 1019 6 233 145
Fragments arranged by size:
1019 233 145 142 6
EcoO109I rG'GnC_Cy
Cuts at: 0 101 990 1545
Size: 101 889 555
Fragments arranged by size:
889 555 101
HaeII r_GCGC'y
Cuts at: 0 607 1545
Size: 607 938
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 1051 1083 1545
Size: 70 32 949 32 462
Fragments arranged by size:
949 462 70 32 32
MfeI C'AATT_G
Cuts at: 0 827 1116 1545
Size: 827 289 429
Fragments arranged by size:
827 429 289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 1545
Size: 1197 348
MscI TGG'CCA
Cuts at: 0 808 1545
Size: 808 737
MslI CAynn'nnrTG
Cuts at: 0 568 622 1545
Size: 568 54 923
Fragments arranged by size:
923 568 54
MspA1I CmG'CkG
Cuts at: 0 120 473 950 1235 1545
Size: 120 353 477 285 310
Fragments arranged by size:
477 353 310 285 120
NcoI C'CATG_G
Cuts at: 0 1124 1545
Size: 1124 421
NdeI CA'TA_TG
Cuts at: 0 520 1300 1545
Size: 520 780 245
Fragments arranged by size:
780 520 245
NsiI A_TGCA'T
Cuts at: 0 1002 1545
Size: 1002 543
NspI r_CATG'y
Cuts at: 0 945 1545
Size: 945 600
PfoI T'CCnGG_A
Cuts at: 0 1420 1545
Size: 1420 125
PmlI CAC'GTG
Cuts at: 0 568 1545
Size: 568 977
PpuMI rG'GwC_Cy
Cuts at: 0 101 1545
Size: 101 1444
PvuII CAG'CTG
Cuts at: 0 120 473 950 1545
Size: 120 353 477 595
Fragments arranged by size:
595 477 353 120
SapI GCTCTTCn'nnn_
Cuts at: 0 271 1545
Size: 271 1274
SmaI CCC'GGG
Cuts at: 0 1042 1545
Size: 1042 503
SmlI C'TyrA_G
Cuts at: 0 1264 1545
Size: 1264 281
StuI AGG'CCT
Cuts at: 0 352 1545
Size: 352 1193
StyI C'CwwG_G
Cuts at: 0 200 593 803 1124 1545
Size: 200 393 210 321 421
Fragments arranged by size:
421 393 321 210 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 213 268 1390 1486 1545
Size: 45 168 55 1122 96 59
Fragments arranged by size:
1122 168 96 59 55 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 1545
Size: 208 32 1305
Fragments arranged by size:
1305 208 32
XbaI T'CTAG_A
Cuts at: 0 345 1545
Size: 345 1200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 621 1545
Size: 207 414 924
Fragments arranged by size:
924 414 207
XmaI C'CCGG_G
Cuts at: 0 1040 1545
Size: 1040 505
Enzymes that do cut:
AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI
ApoI AseI AvaI BanI BanII BcgI BfrBI BglI
BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtgI BtgZI
BtsI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PfoI PmlI PpuMI PvuII SapI SmaI SmlI
StuI StyI TaqII TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BclI BmgBI BmrI BmtI BsaBI BseRI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XhoI XmnI ZraI