Restriction map for pF1KSDA0239
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

Acc65I      AclI        AcsI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BbeI        BbrPI       BciVI       BlnI        
BsaBI       BsiEI       BsiWI       Bsp1407I    BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FseI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        1378  GACGTC               GACGT^C
AccI         1270  GTCGAC               GT^[AC][GT]AC
AccIII        158  TCCGGA               T^CCGGA
AcyI         1378  GACGTC               G[GA]^CG[CT]C
AflII         460  CTTAAG               C^TTAAG
ApaI         1101  GGGCCC               GGGCC^C
Asp700I      1398  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1175  TGCGCA               TGC^GCA
BclI          170  TGATCA               T^GATCA
BfrI          460  CTTAAG               C^TTAAG
BglII         893  AGATCT               A^GATCT
BsaAI        1106  CACGTA               [CT]AC^GT[GA]
BsaHI        1378  GACGTC               G[GA]^CG[CT]C
BsaWI         158  TCCGGA               [AT]^CCGG[AT]
BseAI         158  TCCGGA               T^CCGGA
BsmBI         559  GAGACG               CGTCTC(1/5)
BsmI          946  GAATGC               GAATGC(1/-1)
BspDI        1573  ATCGAT               AT^CGAT
BspEI         158  TCCGGA               T^CCGGA
BsrBI        1243  GAGCGG               CCGCTC(-3/-3)
BstXI        2079  CCAAGTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         1573  ATCGAT               AT^CGAT
DrdI         2068  GACTCAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          170  TGATCA               T^GATCA
FspI         1175  TGCGCA               TGC^GCA
Hin1I        1378  GACGTC               G[GA]^CG[CT]C
HincII       1270  GTCGAC               GT[CT]^[GA]AC
HindII       1270  GTCGAC               GT[CT]^[GA]AC
MroI          158  TCCGGA               T^CCGGA
MseI          461  TTAA                 T^TAA
NcoI          238  CCATGG               C^CATGG
PshAI         607  GACGTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          442  GAGCTC               GAGCT^C
SalI         1270  GTCGAC               G^TCGAC
SapI         1401  GCTCTTC              GCTCTTC(1/4)
ScaI         1286  AGTACT               AGT^ACT
SexAI        1758  ACCTGGT              A^CC[AT]GGT
SmaI         2321  CCCGGG               CCC^GGG
SphI          685  GCATGC               GCATG^C
TfiI          843  GATTC                G^A[AT]TC
Tru9I         461  TTAA                 T^TAA
XcmI          907  CCAGCCAGCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2321  CCCGGG               C^CCGGG
XmaI         2321  CCCGGG               C^CCGGG
XmnI         1398  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII        608  ACGTGT               A^C[GA][CT]GT
              733  ACGTGT              
AocI          622  CCTGAGG              CC^T[ACGT]AGG
              847  CCTGAGG             
AspHI         442  GAGCTC               G[AT]GC[AT]^C
             1087  GAGCAC              
BamHI         311  GGATCC               G^GATCC
              695  GGATCC              
BbsI         1878  GAAGAC               GAAGAC(2/6)
              649  GTCTTC              
BbvCI        1231  GCTGAGG              CCTCAGC(-5/-2)
             1258  GCTGAGG             
BcgI          420  CGAGATTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1671  CGAGGACCCTGC        
BglI          384  GCCTGATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1142  GCCAGGCTGGC         
BpuAI        1878  GAAGAC               GAAGAC(2/6)
              649  GTCTTC              
BsaI          523  GAGACC               GGTCTC(1/5)
             2035  GAGACC              
BsiHKAI       442  GAGCTC               G[AT]GC[AT]^C
             1087  GAGCAC              
BsrFI        1227  GCCGGC               [GA]^CCGG[CT]
             2117  GCCGGC              
Bsu36I        622  CCTGAGG              CC^T[ACGT]AGG
              847  CCTGAGG             
Cfr10I       1227  GCCGGC               [GA]^CCGG[CT]
             2117  GCCGGC              
Eco81I        622  CCTGAGG              CC^T[ACGT]AGG
              847  CCTGAGG             
EcoT14I       238  CCATGG               C^C[AT][AT]GG
             2216  CCAAGG              
HgaI          561  GACGC                GACGC(5/10)
              986  GCGTC               
NaeI         1227  GCCGGC               GCC^GGC
             2117  GCCGGC              
NgoMIV       1227  GCCGGC               G^CCGGC
             2117  GCCGGC              
SmlI          460  CTTAAG               C^T[CT][GA]AG
              700  CTCAAG              
StuI         1551  AGGCCT               AGG^CCT
             2170  AGGCCT              
StyI          238  CCATGG               C^C[AT][AT]GG
             2216  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoO65I     
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com