Restriction map for
pF1KSDA0239
(Circular) (Six-base) MAPSORT of: pF1KSDA0239.seq Check: 3042 from: 1 to: 5507
pF1KSDA0239 5507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 384 1093 1649 384
Size: 709 556 4242
Fragments arranged by size:
4242 709 556
AatII G_ACGT'C
Cuts at: 1508 1508
Size: 5507
AccI GT'mk_AC
Cuts at: 1397 2545 1397
Size: 1148 4359
Acc65I G'GTAC_C
Cuts at: 2523 2523
Size: 5507
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1543 2306 3332 3764 4511 4779 1444
Size: 99 763 1026 432 747 268 2172
Fragments arranged by size:
2172 1026 763 747 432 268 99
AflII C'TTAA_G
Cuts at: 586 586
Size: 5507
AflIII A'CryG_T
Cuts at: 734 859 2859 3963 4940 734
Size: 125 2000 1104 977 1301
Fragments arranged by size:
2000 1301 1104 977 125
AgeI A'CCGG_T
Cuts at: 3895 3895
Size: 5507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 228 416 505 1939 228
Size: 188 89 1434 3796
Fragments arranged by size:
3796 1434 188 89
AlwNI CAG_nnn'CTG
Cuts at: 1043 1064 1314 1452 1668 2188 2812 4379 1043
Size: 21 250 138 216 520 624 1567 2171
Fragments arranged by size:
2171 1567 624 520 250 216 138 21
ApaI G_GGCC'C
Cuts at: 1231 1231
Size: 5507
ApaLI G'TGCA_C
Cuts at: 4277 4277
Size: 5507
ApoI r'AATT_y
Cuts at: 2511 4860 5116 2511
Size: 2349 256 2902
Fragments arranged by size:
2902 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 477 1907 2447 2527 477
Size: 1430 540 80 3457
Fragments arranged by size:
3457 1430 540 80
BamHI G'GATC_C
Cuts at: 437 821 2532 437
Size: 384 1711 3412
Fragments arranged by size:
3412 1711 384
BanI G'GyrC_C
Cuts at: 396 831 1081 1705 1790 2290 2523 2937 3185
Size: 435 250 624 85 500 233 414 248
Cuts at: 3185 3220 396
Size: 35 2683
Fragments arranged by size:
2683 624 500 435 414 250 248 233
85 35
BanII G_rGCy'C
Cuts at: 428 505 572 1010 1050 1231 1244 1256 2521
Size: 77 67 438 40 181 13 12 1265
Cuts at: 2521 3551 428
Size: 1030 2384
Fragments arranged by size:
2384 1265 1030 438 181 77 67 40
13 12
BbeI G_GCGC'C
Cuts at: 2941 3189 2941
Size: 248 5259
BbsI GAAGACnn'nnnn_
Cuts at: 768 2011 768
Size: 1243 4264
BbvCI CC'TCA_GC
Cuts at: 1358 1385 1358
Size: 27 5480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 569 1786 1820 535
Size: 34 1217 34 4222
Fragments arranged by size:
4222 1217 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3405 4166 5507 3405
Size: 761 1341 3405
Fragments arranged by size:
3405 1341 761
BclI T'GATC_A
Cuts at: 296 3027 296
Size: 2731 2776
BglI GCCn_nnn'nGGC
Cuts at: 516 1274 516
Size: 758 4749
BglII A'GATC_T
Cuts at: 1019 3022 5059 1019
Size: 2003 2037 1467
Fragments arranged by size:
2037 2003 1467
BlpI GC'TnA_GC
Cuts at: 331 1367 1855 2619 331
Size: 1036 488 764 3219
Fragments arranged by size:
3219 1036 764 488
Bme1580I G_kGCm'C
Cuts at: 836 867 963 1231 1793 3132 3225 4281 836
Size: 31 96 268 562 1339 93 1056 2062
Fragments arranged by size:
2062 1339 1056 562 268 96 93 31
BmgBI CAC'GTC
Cuts at: 735 860 2071 735
Size: 125 1211 4171
Fragments arranged by size:
4171 1211 125
BmrI ACTGGGnnnn_n'
Cuts at: 2719 2800 3135 5279 2719
Size: 81 335 2144 2947
Fragments arranged by size:
2947 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2196 3874 2196
Size: 1678 3829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 658 667 1606 1793 1970 586
Size: 72 9 939 187 177 4123
Fragments arranged by size:
4123 939 187 177 72 9
Bpu10I CC'TnA_GC
Cuts at: 1358 1385 2231 1358
Size: 27 846 4634
Fragments arranged by size:
4634 846 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2683 3562 4054 4352 811
Size: 1872 879 492 298 1966
Fragments arranged by size:
1966 1872 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 2155 5211 643
Size: 1512 3056 939
Fragments arranged by size:
3056 1512 939
BsaAI yAC'GTr
Cuts at: 1234 2499 2860 1234
Size: 1265 361 3881
Fragments arranged by size:
3881 1265 361
BsaBI GATnn'nnATC
Cuts at: 6 3021 5058 6
Size: 3015 2037 455
Fragments arranged by size:
3015 2037 455
BsaHI Gr'CG_yC
Cuts at: 1505 2938 3186 3888 5384 1505
Size: 1433 248 702 1496 1628
Fragments arranged by size:
1628 1496 1433 702 248
BsaWI w'CCGG_w
Cuts at: 284 2696 3217 3895 4169 4316 4727 284
Size: 2412 521 678 274 147 411 1064
Fragments arranged by size:
2412 1064 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 5477
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 655 920 923 1342 1858 2479 655
Size: 265 3 419 516 621 3683
Fragments arranged by size:
3683 621 516 419 265 3
BseYI C'CCAG_C
Cuts at: 226 506 699 1032 1043 1264 1490 2305 2932
Size: 280 193 333 11 221 226 815 627
Cuts at: 2932 3690 4267 4763 226
Size: 758 577 496 970
Fragments arranged by size:
970 815 758 627 577 496 333 280
226 221 193 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1759 2455 412
Size: 1347 696 3464
Fragments arranged by size:
3464 1347 696
BsiEI CG_ry'CG
Cuts at: 104 3049 3095 4303 104
Size: 2945 46 1208 1308
Fragments arranged by size:
2945 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 572 1217 2521 3299 3489 4281 572
Size: 645 1304 778 190 792 1798
Fragments arranged by size:
1798 1304 792 778 645 190
BsmI GAATG_Cn'
Cuts at: 1078 1078
Size: 5507
BsmBI CGTCTCn'nnnn_
Cuts at: 679 679
Size: 5507
Bsp1286I G_dGCh'C
Cuts at: 428 505 572 836 867 963 1010 1050 1217
Size: 77 67 264 31 96 47 40 167
Cuts at: 1217 1231 1244 1256 1793 2521 3132 3225 3299
Size: 14 13 12 537 728 611 93 74
Cuts at: 3299 3489 3551 4281 428
Size: 190 62 730 1654
Fragments arranged by size:
1654 730 728 611 537 264 190 167
96 93 77 74 67 62 47 40
31 14 13 12
BspEI T'CCGG_A
Cuts at: 284 4727 284
Size: 4443 1064
BspHI T'CATG_A
Cuts at: 4683 4683
Size: 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 384 1093 1649 2557 2771 3073 3454 384
Size: 709 556 908 214 302 381 2437
Fragments arranged by size:
2437 908 709 556 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1371 3799 3853 5337 5502 50
Size: 1321 2428 54 1484 165 55
Fragments arranged by size:
2428 1484 1321 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3419 3419
Size: 5507
BsrFI r'CCGG_y
Cuts at: 1353 2243 3505 3686 3895 1353
Size: 890 1262 181 209 2965
Fragments arranged by size:
2965 1262 890 209 181
BssSI C'ACGA_G
Cuts at: 3778 4136 3778
Size: 358 5149
BstAPI GCAn_nnn'nTGC
Cuts at: 989 1064 1073 1314 2884 989
Size: 75 9 241 1570 3612
Fragments arranged by size:
3612 1570 241 75 9
BstBI TT'CG_AA
Cuts at: 110 3868 110
Size: 3758 1749
BstEII G'GTnAC_C
Cuts at: 1293 1888 2271 1293
Size: 595 383 4529
Fragments arranged by size:
4529 595 383
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2212
Size: 5507
BstYI r'GATC_y
Cuts at: 1 311 437 821 1019 1665 2532 2999 3022
Size: 310 126 384 198 646 867 467 23
Cuts at: 3022 3357 3603 4615 4701 4713 5059 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 867 646 467 449 384 346 335
310 246 198 126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 749 974 749
Size: 225 5282
BtgI C'CryG_G
Cuts at: 125 364 428 836 1146 1256 125
Size: 239 64 408 310 110 4376
Fragments arranged by size:
4376 408 310 239 110 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 165 1849 2034 2750 165
Size: 1684 185 716 2922
Fragments arranged by size:
2922 1684 716 185
ClaI AT'CG_AT
Cuts at: 1700 1700
Size: 5507
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 2200 3213 4071 2200
Size: 1013 858 3636
Fragments arranged by size:
3636 1013 858
EaeI y'GGCC_r
Cuts at: 517 671 1760 1774 2251 3092 3266 3657 3684
Size: 154 1089 14 477 841 174 391 27
Cuts at: 3684 5029 517
Size: 1345 995
Fragments arranged by size:
1345 1089 995 841 477 391 174 154
27 14
EagI C'GGCC_G
Cuts at: 3092 3092
Size: 5507
EarI CTCTTCn'nnn_
Cuts at: 125 130 1420 1534 3530 3740 125
Size: 5 1290 114 1996 210 1892
Fragments arranged by size:
1996 1892 1290 210 114 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3715 4025 4171 5317 3715
Size: 310 146 1146 3905
Fragments arranged by size:
3905 1146 310 146
EcoICRI GAG'CTC
Cuts at: 570 2519 570
Size: 1949 3558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 658 667 1444 1543 1606 1793 1970 2306
Size: 72 9 777 99 63 187 177 336
Cuts at: 2306 3332 3764 4511 4779 586
Size: 1026 432 747 268 1314
Fragments arranged by size:
1314 1026 777 747 432 336 268 187
177 99 72 63 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1066 1288 1384 1808 1066
Size: 222 96 424 4765
Fragments arranged by size:
4765 424 222 96
EcoO109I rG'GnC_Cy
Cuts at: 457 1227 1281 1548 1800 1869 2443 2646 457
Size: 770 54 267 252 69 574 203 3318
Fragments arranged by size:
3318 770 574 267 252 203 69 54
EcoRI G'AATT_C
Cuts at: 2511 2511
Size: 5507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 1303 3288 1303
Size: 1985 3522
HaeII r_GCGC'y
Cuts at: 2941 3189 4211 5190 2941
Size: 248 1022 979 3258
Fragments arranged by size:
3258 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1263 2067 2099 3349 3379 3381 3411 1231
Size: 32 804 32 1250 30 2 30 3327
Fragments arranged by size:
3327 1250 804 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1398 2546 1398
Size: 1148 4359
KasI G'GCGC_C
Cuts at: 2937 3185 2937
Size: 248 5259
KpnI G_GTAC'C
Cuts at: 2527 2527
Size: 5507
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2053 4178 4362 2053
Size: 2125 184 3198
Fragments arranged by size:
3198 2125 184
MscI TGG'CCA
Cuts at: 519 673 1762 1776 3268 519
Size: 154 1089 14 1492 2758
Fragments arranged by size:
2758 1492 1089 154 14
MslI CAynn'nnrTG
Cuts at: 210 304 1762 3496 3623 210
Size: 94 1458 1734 127 2094
Fragments arranged by size:
2094 1734 1458 127 94
MspA1I CmG'CkG
Cuts at: 845 956 1043 1311 1452 1721 2326 2439 2618
Size: 111 87 268 141 269 605 113 179
Cuts at: 2618 2932 3292 4305 4550 845
Size: 314 360 1013 245 1802
Fragments arranged by size:
1802 1013 605 360 314 269 268 245
179 141 113 111 87
NaeI GCC'GGC
Cuts at: 1355 2245 3688 1355
Size: 890 1443 3174
Fragments arranged by size:
3174 1443 890
NarI GG'CG_CC
Cuts at: 2938 3186 2938
Size: 248 5259
NcoI C'CATG_G
Cuts at: 125 364 125
Size: 239 5268
NgoMIV G'CCGG_C
Cuts at: 1353 2243 3686 1353
Size: 890 1443 3174
Fragments arranged by size:
3174 1443 890
NspI r_CATG'y
Cuts at: 815 952 1136 2560 3060 3967 815
Size: 137 184 1424 500 907 2355
Fragments arranged by size:
2355 1424 907 500 184 137
PasI CC'CwG_GG
Cuts at: 867 867
Size: 5507
PciI A'CATG_T
Cuts at: 3963 3963
Size: 5507
PflMI CCAn_nnn'nTGG
Cuts at: 669 1043 1152 669
Size: 374 109 5024
Fragments arranged by size:
5024 374 109
PfoI T'CCnGG_A
Cuts at: 314 1622 314
Size: 1308 4199
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1177 1209 4646 4678 1177
Size: 32 3437 32 2006
Fragments arranged by size:
3437 2006 32 32
PpuMI rG'GwC_Cy
Cuts at: 1281 1548 1800 1869 2443 1281
Size: 267 252 69 574 4345
Fragments arranged by size:
4345 574 267 252 69
PshAI GACnn'nnGTC
Cuts at: 737 737
Size: 5507
PspOMI G'GGCC_C
Cuts at: 1227 1227
Size: 5507
PstI C_TGCA'G
Cuts at: 1060 1319 1646 1838 2106 2223 2554 3239 1060
Size: 259 327 192 268 117 331 685 3328
Fragments arranged by size:
3328 685 331 327 268 259 192 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 845 956 1721 2326 2932 3292 845
Size: 111 765 605 606 360 3060
Fragments arranged by size:
3060 765 606 605 360 111
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 572 2521 572
Size: 1949 3558
SalI G'TCGA_C
Cuts at: 1396 2544 1396
Size: 1148 4359
SanDI GG'GwC_CC
Cuts at: 1869 2443 1869
Size: 574 4933
SapI GCTCTTCn'nnn_
Cuts at: 1534 3530 3740 1534
Size: 1996 210 3301
Fragments arranged by size:
3301 1996 210
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 1414 5024 1414
Size: 3610 1897
SexAI A'CCwGG_T
Cuts at: 1884 1884
Size: 5507
SfcI C'TryA_G
Cuts at: 33 1056 1315 1642 1834 2102 2219 2550 3235
Size: 1023 259 327 192 268 117 331 685
Cuts at: 3235 4228 4419 33
Size: 993 191 1121
Fragments arranged by size:
1121 1023 993 685 331 327 268 259
192 191 117
SfoI GGC'GCC
Cuts at: 2939 3187 2939
Size: 248 5259
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2449 2529 2449
Size: 80 5427
SmlI C'TyrA_G
Cuts at: 586 826 2662 3577 4069 4331 586
Size: 240 1836 915 492 262 1762
Fragments arranged by size:
1836 1762 915 492 262 240
SnaBI TAC'GTA
Cuts at: 2499 2499
Size: 5507
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 815 2560 3060 815
Size: 1745 500 3262
Fragments arranged by size:
3262 1745 500
StuI AGG'CCT
Cuts at: 1679 2298 1679
Size: 619 4888
StyI C'CwwG_G
Cuts at: 125 364 2342 2641 2995 125
Size: 239 1978 299 354 2637
Fragments arranged by size:
2637 1978 354 299 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 859 933 1483 1549 3090 3223 3682 3891 859
Size: 74 550 66 1541 133 459 209 2475
Fragments arranged by size:
2475 1541 550 459 209 133 74 66
TatI w'GTAC_w
Cuts at: 1412 5022 1412
Size: 3610 1897
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 640 672 2077 2109 5024 5056 640
Size: 32 1405 32 2915 32 1091
Fragments arranged by size:
2915 1405 1091 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3304 3304
Size: 5507
XbaI T'CTAG_A
Cuts at: 2538 2538
Size: 5507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1040 3002 1040
Size: 1962 3545
XmaI C'CCGG_G
Cuts at: 2447 2527 2447
Size: 80 5427
XmnI GAAnn'nnTTC
Cuts at: 1528 1528
Size: 5507
ZraI GAC'GTC
Cuts at: 1506 1506
Size: 5507
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspXI PsrI SacII
SfiI SgrAI SrfI SspI SwaI XhoI