Restriction map for pF1KSDA0235
 (Linear) (Six-base) MAPSORT of: KIAA0235.seq  Check: 2475  from: 1  to: 3192



KIAA0235.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AccI GT'mk_AC

Cuts at:      0     970    2793    3192 
   Size:        970    1823     399

  Fragments arranged by size: 

                1823     970     399
 
Acc65I G'GTAC_C

Cuts at:      0     729    3192 
   Size:        729    2463
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     728    2149    2819    3192 
   Size:        572     156    1421     670     373

  Fragments arranged by size: 

                1421     670     572     373     156
 
AfeI AGC'GCT

Cuts at:      0     565    3192 
   Size:        565    2627
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     496     866    1100    1567    1891    2702    3192 
   Size:        496     370     234     467     324     811     490

  Fragments arranged by size: 

                 811     496     490     467     370     324     234
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1364    1396    3192 
   Size:       1364      32    1796

  Fragments arranged by size: 

                1796    1364      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     669    1450    1999    3192 
   Size:        669     781     549    1193

  Fragments arranged by size: 

                1193     781     669     549
 
ApaI G_GGCC'C

Cuts at:      0     574    3192 
   Size:        574    2618
 
ApoI r'AATT_y

Cuts at:      0     342     643    2285    2860    3192 
   Size:        342     301    1642     575     332

  Fragments arranged by size: 

                1642     575     342     332     301
 
AseI AT'TA_AT

Cuts at:      0    2308    3192 
   Size:       2308     884
 
AvaI C'yCGr_G

Cuts at:      0      35    3192 
   Size:         35    3157
 
AvrII C'CTAG_G

Cuts at:      0    3156    3192 
   Size:       3156      36
 
BamHI G'GATC_C

Cuts at:      0    1933    3192 
   Size:       1933    1259
 
BanI G'GyrC_C

Cuts at:      0     729    1333    3192 
   Size:        729     604    1859

  Fragments arranged by size: 

                1859     729     604
 
BanII G_rGCy'C

Cuts at:      0     574    1377    3192 
   Size:        574     803    1815

  Fragments arranged by size: 

                1815     803     574
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2807    3192 
   Size:       2807     385
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3046    3080    3192 
   Size:       3046      34     112

  Fragments arranged by size: 

                3046     112      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1067    3192 
   Size:       1067    2125
 
BclI T'GATC_A

Cuts at:      0    1323    3192 
   Size:       1323    1869
 
BfrBI ATG'CAT

Cuts at:      0     853    3192 
   Size:        853    2339
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1475    3192 
   Size:       1475    1717
 
BglII A'GATC_T

Cuts at:      0     196    1789    3192 
   Size:        196    1593    1403

  Fragments arranged by size: 

                1593    1403     196
 
BlpI GC'TnA_GC

Cuts at:      0     866    1133    1692    2261    3192 
   Size:        866     267     559     569     931

  Fragments arranged by size: 

                 931     866     569     559     267
 
Bme1580I G_kGCm'C

Cuts at:      0     574    3192 
   Size:        574    2618
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     607    3192 
   Size:        607    2585
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1775    2270    3192 
   Size:       1775     495     922

  Fragments arranged by size: 

                1775     922     495
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     893     938    1184    1361    1373    1472    1874    2021 
   Size:        893      45     246     177      12      99     402     147

Cuts at:   2021    2037    2240    3192 
   Size:         16     203     952

  Fragments arranged by size: 

                 952     893     402     246     203     177     147      99
                  45      16      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    2415    3192 
   Size:       2415     777
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2189    3192 
   Size:       2189    1003
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     425    3192 
   Size:        425    2767
 
BsaAI yAC'GTr

Cuts at:      0    3003    3192 
   Size:       3003     189
 
BsaBI GATnn'nnATC

Cuts at:      0     515    1938    3192 
   Size:        515    1423    1254

  Fragments arranged by size: 

                1423    1254     515
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     740     770    1139    1169    1364    1394    3192 
   Size:        740      30     369      30     195      30    1798

  Fragments arranged by size: 

                1798     740     369     195      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     941    1839    3192 
   Size:        430     511     898    1353

  Fragments arranged by size: 

                1353     898     511     430
 
BseYI C'CCAG_C

Cuts at:      0     173     779    3192 
   Size:        173     606    2413

  Fragments arranged by size: 

                2413     606     173
 
BsiEI CG_ry'CG

Cuts at:      0    2793    3192 
   Size:       2793     399
 
BsiHKAI G_wGCw'C

Cuts at:      0    1377    2519    3192 
   Size:       1377    1142     673

  Fragments arranged by size: 

                1377    1142     673
 
BsmI GAATG_Cn'

Cuts at:      0    3191    3192 
   Size:       3191       1
 
Bsp1286I G_dGCh'C

Cuts at:      0     574    1377    2519    3192 
   Size:        574     803    1142     673

  Fragments arranged by size: 

                1142     803     673     574
 
BspHI T'CATG_A

Cuts at:      0       6    3192 
   Size:          6    3186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     997    1159    3042    3192 
   Size:        997     162    1883     150

  Fragments arranged by size: 

                1883     997     162     150
 
BsrDI GCAATG_nn'

Cuts at:      0    2679    2877    3192 
   Size:       2679     198     315

  Fragments arranged by size: 

                2679     315     198
 
BsrGI T'GTAC_A

Cuts at:      0    1781    2553    3192 
   Size:       1781     772     639

  Fragments arranged by size: 

                1781     772     639
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     904    1432    3192 
   Size:        904     528    1760

  Fragments arranged by size: 

                1760     904     528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2950    3003    3192 
   Size:       2950      53     189

  Fragments arranged by size: 

                2950     189      53
 
BstYI r'GATC_y

Cuts at:      0     196    1789    1933    3192 
   Size:        196    1593     144    1259

  Fragments arranged by size: 

                1593    1259     196     144
 
BstZ17I GTA'TAC

Cuts at:      0     971    3192 
   Size:        971    2221
 
BtgI C'CryG_G

Cuts at:      0     677    1045    3192 
   Size:        677     368    2147

  Fragments arranged by size: 

                2147     677     368
 
BtsI GCAGTG_nn'

Cuts at:      0    1022    2684    3192 
   Size:       1022    1662     508

  Fragments arranged by size: 

                1662    1022     508
 
EaeI y'GGCC_r

Cuts at:      0    1498    2115    3116    3192 
   Size:       1498     617    1001      76

  Fragments arranged by size: 

                1498    1001     617      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     611     747    3192 
   Size:        611     136    2445

  Fragments arranged by size: 

                2445     611     136
 
EcoICRI GAG'CTC

Cuts at:      0    1375    3192 
   Size:       1375    1817
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     728     893     938    1184    1361    1373    1472 
   Size:        572     156     165      45     246     177      12      99

Cuts at:   1472    1874    2021    2037    2149    2240    2819    3192 
   Size:        402     147      16     112      91     579     373

  Fragments arranged by size: 

                 579     572     402     373     246     177     165     156
                 147     112      99      91      45      16      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     380    3167    3192 
   Size:        380    2787      25

  Fragments arranged by size: 

                2787     380      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     571     601    3160    3192 
   Size:        571      30    2559      32

  Fragments arranged by size: 

                2559     571      32      30
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1598    3192 
   Size:       1598    1594
 
FspI TGC'GCA

Cuts at:      0    3088    3192 
   Size:       3088     104
 
HaeII r_GCGC'y

Cuts at:      0     567    3192 
   Size:        567    2625
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1747    1779    2352    2384    3054    3086    3192 
   Size:       1747      32     573      32     670      32     106

  Fragments arranged by size: 

                1747     670     573     106      32      32      32
 
HincII GTy'rAC

Cuts at:      0     827    1633    2794    2854    3192 
   Size:        827     806    1161      60     338

  Fragments arranged by size: 

                1161     827     806     338      60
 
HpaI GTT'AAC

Cuts at:      0     827    3192 
   Size:        827    2365
 
KpnI G_GTAC'C

Cuts at:      0     733    3192 
   Size:        733    2459
 
MfeI C'AATT_G

Cuts at:      0    1502    3192 
   Size:       1502    1690
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1273    1333    1420    3104    3192 
   Size:        571     702      60      87    1684      88

  Fragments arranged by size: 

                1684     702     571      88      87      60
 
MscI TGG'CCA

Cuts at:      0    3118    3192 
   Size:       3118      74
 
MslI CAynn'nnrTG

Cuts at:      0    2645    2977    3192 
   Size:       2645     332     215

  Fragments arranged by size: 

                2645     332     215
 
MspA1I CmG'CkG

Cuts at:      0     913    1090    1234    1450    2260    3192 
   Size:        913     177     144     216     810     932

  Fragments arranged by size: 

                 932     913     810     216     177     144
 
NcoI C'CATG_G

Cuts at:      0     677    1045    3192 
   Size:        677     368    2147

  Fragments arranged by size: 

                2147     677     368
 
NsiI A_TGCA'T

Cuts at:      0     855    3192 
   Size:        855    2337
 
NspI r_CATG'y

Cuts at:      0    1287    1872    2779    3192 
   Size:       1287     585     907     413

  Fragments arranged by size: 

                1287     907     585     413
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3181    3192 
   Size:       3181      11
 
PfoI T'CCnGG_A

Cuts at:      0     525    1890    3192 
   Size:        525    1365    1302

  Fragments arranged by size: 

                1365    1302     525
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1364    1396    3192 
   Size:       1364      32    1796

  Fragments arranged by size: 

                1796    1364      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3160    3192 
   Size:       3160      32
 
PspOMI G'GGCC_C

Cuts at:      0     570    3192 
   Size:        570    2622
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2684    2716    3192 
   Size:       2684      32     476

  Fragments arranged by size: 

                2684     476      32
 
PstI C_TGCA'G

Cuts at:      0     834     978     993    1089    1239    1437    1995    2656 
   Size:        834     144      15      96     150     198     558     661

Cuts at:   2656    2691    3192 
   Size:         35     501

  Fragments arranged by size: 

                 834     661     558     501     198     150     144      96
                  35      15
 
PvuII CAG'CTG

Cuts at:      0     913    1090    1234    1450    2260    3192 
   Size:        913     177     144     216     810     932

  Fragments arranged by size: 

                 932     913     810     216     177     144
 
SacI G_AGCT'C

Cuts at:      0    1377    3192 
   Size:       1377    1815
 
SalI G'TCGA_C

Cuts at:      0    2792    3192 
   Size:       2792     400
 
ScaI AGT'ACT

Cuts at:      0    1301    3192 
   Size:       1301    1891
 
SexAI A'CCwGG_T

Cuts at:      0    1626    3192 
   Size:       1626    1566
 
SfcI C'TryA_G

Cuts at:      0     617     755     830     974     989    1085    1235    1433 
   Size:        617     138      75     144      15      96     150     198

Cuts at:   1433    1685    1991    2590    2652    2687    3192 
   Size:        252     306     599      62      35     505

  Fragments arranged by size: 

                 617     599     505     306     252     198     150     144
                 138      96      75      62      35      15
 
SmlI C'TyrA_G

Cuts at:      0    2204    3192 
   Size:       2204     988
 
SphI G_CATG'C

Cuts at:      0    1287    1872    3192 
   Size:       1287     585    1320

  Fragments arranged by size: 

                1320    1287     585
 
StuI AGG'CCT

Cuts at:      0     266     761     902    3192 
   Size:        266     495     141    2290

  Fragments arranged by size: 

                2290     495     266     141
 
StyI C'CwwG_G

Cuts at:      0     267     677    1045    1368    3156    3192 
   Size:        267     410     368     323    1788      36

  Fragments arranged by size: 

                1788     410     368     323     267      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     976    1666    1824    3192 
   Size:        976     690     158    1368

  Fragments arranged by size: 

                1368     976     690     158
 
TatI w'GTAC_w

Cuts at:      0    1299    1642    1781    2553    2778    3192 
   Size:       1299     343     139     772     225     414

  Fragments arranged by size: 

                1299     772     414     343     225     139
 
Tth111I GACn'n_nGTC

Cuts at:      0    1628    3192 
   Size:       1628    1564
 
XbaI T'CTAG_A

Cuts at:      0    2221    3192 
   Size:       2221     971
 
XmnI GAAnn'nnTTC

Cuts at:      0    1578    2025    2573    3192 
   Size:       1578     447     548     619

  Fragments arranged by size: 

                1578     619     548     447
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrGI   BstAPI    BstXI    BstYI  BstZ17I     BtgI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII     HpaI     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI    PflMI     PfoI
     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI     SalI
     ScaI    SexAI     SfcI     SmlI     SphI     StuI     StyI    TaqII
     TatI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI
    ApaLI     AscI    AsiSI     BaeI     BbeI    BbvCI    BmgBI     BmtI
    BsaHI    BsaWI     BsgI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FseI
    FspAI  HindIII     KasI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
    PshAI     PsiI    PspXI     PvuI    RsrII    SacII    SanDI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     TstI     XcmI     XhoI     XmaI     ZraI
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