Restriction map for pF1KSDA0223
##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AclI        AgeI        AocI        AscI        AseI        
AsnI        Asp700I     AvrII       BamHI       BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrGI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eco81I      EcoO65I     
EcoRV       FbaI        FseI        HincII      HindII      
HindIII     HpaI        MamI        MfeI        MunI        
NheI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       1948  GGTACC               G^GTACC
AccIII       2114  TCCGGA               T^CCGGA
AcsI         3400  GAATTC               [GA]^AATT[CT]
AflII         397  CTTAAG               C^TTAAG
AflIII       2409  ACGCGT               A^C[GA][CT]GT
AhdI          974  GACAGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         3400  GAATTC               [GA]^AATT[CT]
Asp718I      1948  GGTACC               G^GTACC
AspEI         974  GACAGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          331  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1551  GGCGCC               GGCGC^C
BbrPI        1627  CACGTG               CAC^GTG
BcgI         2792  GCATCGTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1443  GGATAC               GTATCC(6/5)
BfrI          397  CTTAAG               C^TTAAG
BsaI         2938  GAGACC               GGTCTC(1/5)
BseAI        2114  TCCGGA               T^CCGGA
BsiEI        1756  CGGCCG               CG[GA][CT]^CG
BspEI        2114  TCCGGA               T^CCGGA
BspHI          26  TCATGA               T^CATGA
BsrDI         549  CATTGC               GCAATG(2/0)
BssHII       1298  GCGCGC               G^CGCGC
BstAPI       2718  GCAGTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1469  AGATCC               [GA]^GATC[CT]
EagI         1756  CGGCCG               C^GGCCG
Eam1105I      974  GACAGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1756  CGGCCG               C^GGCCG
Eco52I       1756  CGGCCG               C^GGCCG
EcoRI        3400  GAATTC               G^AATTC
KasI         1551  GGCGCC               G^GCGCC
KpnI         1948  GGTACC               GGTAC^C
KspI          442  CCGCGG               CCGC^GG
MflI         1469  AGATCC               [GA]^GATC[CT]
MluI         2409  ACGCGT               A^CGCGT
MroI         2114  TCCGGA               T^CCGGA
MslI         1401  CACCTGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1551  GGCGCC               GG^CGCC
NcoI          725  CCATGG               C^CATGG
NdeI         1540  CATATG               CA^TATG
NspI          998  ACATGC               [GA]CATG^[CT]
PaeR7I        714  CTCGAG               C^TCGAG
PflFI         331  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1627  CACGTG               CAC^GTG
PmlI         1627  CACGTG               CAC^GTG
RcaI           26  TCATGA               T^CATGA
SacII         442  CCGCGG               CCGC^GG
SapI         1725  GAAGAGC              GCTCTTC(1/4)
SbfI         3380  CCTGCAGG             CCTGCA^GG
SexAI        1841  ACCAGGT              A^CC[AT]GGT
SfoI         1551  GGCGCC               GGC^GCC
Sse8387I     3380  CCTGCAGG             CCTGCA^GG
Tsp509I      3401  AATT                 ^AATT
Tth111I       331  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          714  CTCGAG               C^TCGAG
XhoII        1469  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AatII         331  GACGTC               GACGT^C
             1935  GACGTC              
AccI         2161  GTCTAC               GT^[AC][GT]AC
             3025  GTCTAC              
AlwI         1470  GATCC                GGATC(4/5)
             2744  GGATC               
Aor51HI       827  AGCGCT               AGC^GCT
             1232  AGCGCT              
ApaI          446  GGGCCC               GGGCC^C
             2803  GGGCCC              
AviII        1205  TGCGCA               TGC^GCA
             2369  TGCGCA              
BsaAI        1225  TACGTG               [CT]AC^GT[GA]
             1627  CACGTG              
BsaWI         272  ACCGGA               [AT]^CCGG[AT]
             2114  TCCGGA              
EarI         1725  GAAGAG               CTCTTC(1/4)
             2185  GAAGAG              
Eco47III      827  AGCGCT               AGC^GCT
             1232  AGCGCT              
EcoT22I       481  ATGCAT               ATGCA^T
             1537  ATGCAT              
FspI         1205  TGCGCA               TGC^GCA
             2369  TGCGCA              
Ksp632I      1725  GAAGAG               CTCTTC(1/4)
             2185  GAAGAG              
MseI          398  TTAA                 T^TAA
             1919  TTAA                
NsiI          481  ATGCAT               ATGCA^T
             1537  ATGCAT              
Tru9I         398  TTAA                 T^TAA
             1919  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwNI       ApaLI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com