Restriction map for pF1KSDA0221
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AccIII      AcsI        AflII       AgeI        AhdI        
AocI        ApoI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BseAI       BsiEI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FspI        HindIII     
HpaI        KspI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
PvuI        RsrII       SacI        SacII       ScaI        
SfuI        SnaBI       SpeI        SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        1533  GACGTC               GACGT^C
Acc65I       1814  GGTACC               G^GTACC
AclI         1317  AACGTT               AA^CGTT
AflIII       1879  ACATGT               A^C[GA][CT]GT
Alw44I       1232  GTGCAC               G^TGCAC
ApaLI        1232  GTGCAC               G^TGCAC
Asp718I      1814  GGTACC               G^GTACC
BalI         1907  TGGCCA               TGG^CCA
BbrPI        2956  CACGTG               CAC^GTG
BclI         2936  TGATCA               T^GATCA
BsaBI        2400  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1333  GAGACC               GGTCTC(1/5)
BsaWI        2138  ACCGGA               [AT]^CCGG[AT]
BsiWI          14  CGTACG               C^GTACG
BspHI        2819  TCATGA               T^CATGA
BspLU11I     1879  ACATGT               A^CATGT
BssSI        1375  CACGAG               CACGAG(-5/-1)
BstXI        2574  CCAAGGCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI         2936  TGATCA               T^GATCA
FseI         2890  GGCCGGCC             GGCCGG^CC
KpnI         1814  GGTACC               GGTAC^C
MamI         2400  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1907  TGGCCA               TGG^CCA
MscI         1907  TGGCCA               TGG^CCA
PmaCI        2956  CACGTG               CAC^GTG
PmlI         2956  CACGTG               CAC^GTG
Psp1406I     1317  AACGTT               AA^CGTT
RcaI         2819  TCATGA               T^CATGA
SalI         1281  GTCGAC               G^TCGAC
SapI         2082  GCTCTTC              GCTCTTC(1/4)
SbfI         2124  CCTGCAGG             CCTGCA^GG
SfiI          148  GGCCCCGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        1890  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         1742  GCATGC               GCATG^C
Sse8387I     2124  CCTGCAGG             CCTGCA^GG
TfiI         1022  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1281  GTCGAC               GT^[AC][GT]AC
             1549  GTCTAC              
Aor51HI      1415  AGCGCT               AGC^GCT
             2429  AGCGCT              
BfaI          122  CTAG                 C^TAG
             2951  CTAG                
BstEII        501  GGTGACC              G^GT[ACGT]ACC
             1894  GGTGACC             
BstPI         501  GGTGACC              G^GT[ACGT]ACC
             1894  GGTGACC             
BstYI         731  AGATCC               [GA]^GATC[CT]
             1631  AGATCC              
DraI         2589  TTTAAA               TTT^AAA
             3292  TTTAAA              
DraIII        475  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1375  CACGAGGTG           
Eco47III     1415  AGCGCT               AGC^GCT
             2429  AGCGCT              
EcoO65I       501  GGTGACC              G^GT[ACGT]ACC
             1894  GGTGACC             
HincII       1281  GTCGAC               GT[CT]^[GA]AC
             2791  GTCAAC              
HindII       1281  GTCGAC               GT[CT]^[GA]AC
             2791  GTCAAC              
MaeI          122  CTAG                 C^TAG
             2951  CTAG                
MflI          731  AGATCC               [GA]^GATC[CT]
             1631  AGATCC              
NaeI         2891  GCCGGC               GCC^GGC
             2944  GCCGGC              
NgoMIV       2891  GCCGGC               G^CCGGC
             2944  GCCGGC              
SexAI        2438  ACCTGGT              A^CC[AT]GGT
             2990  ACCTGGT             
SmlI          655  CTCAAG               C^T[CT][GA]AG
              974  CTCAAG              
Tsp509I      1077  AATT                 ^AATT
             1189  AATT                
XhoII         731  AGATCC               [GA]^GATC[CT]
             1631  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI        XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com