Restriction map for pF1KSDA0218
 (Linear) (Six-base) MAPSORT of: KIAA0218.seq  Check: 2266  from: 1  to: 2283



KIAA0218.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     485     583    1046    2023    2283 
   Size:        317     168      98     463     977     260

  Fragments arranged by size: 

                 977     463     317     260     168      98
 
AflII C'TTAA_G

Cuts at:      0     409    1746    2283 
   Size:        409    1337     537

  Fragments arranged by size: 

                1337     537     409
 
AflIII A'CryG_T

Cuts at:      0    2018    2283 
   Size:       2018     265
 
AleI CACnn'nnGTG

Cuts at:      0    2038    2283 
   Size:       2038     245
 
AlwNI CAG_nnn'CTG

Cuts at:      0     730    2283 
   Size:        730    1553
 
ApaI G_GGCC'C

Cuts at:      0     146     271    2283 
   Size:        146     125    2012

  Fragments arranged by size: 

                2012     146     125
 
ApaLI G'TGCA_C

Cuts at:      0    1803    2283 
   Size:       1803     480
 
ApoI r'AATT_y

Cuts at:      0     454    1329    1559    1585    2283 
   Size:        454     875     230      26     698

  Fragments arranged by size: 

                 875     698     454     230      26
 
AvaI C'yCGr_G

Cuts at:      0     317     623    2069    2175    2283 
   Size:        317     306    1446     106     108

  Fragments arranged by size: 

                1446     317     306     108     106
 
BamHI G'GATC_C

Cuts at:      0     340    2283 
   Size:        340    1943
 
BanI G'GyrC_C

Cuts at:      0    1751    2283 
   Size:       1751     532
 
BanII G_rGCy'C

Cuts at:      0      86     146     217     271     547     860    1997    2283 
   Size:         86      60      71      54     276     313    1137     286

  Fragments arranged by size: 

                1137     313     286     276      86      71      60      54
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323    2283 
   Size:        323    1960
 
BbvCI CC'TCA_GC

Cuts at:      0     470    2283 
   Size:        470    1813
 
BglI GCCn_nnn'nGGC

Cuts at:      0     625    2065    2283 
   Size:        625    1440     218

  Fragments arranged by size: 

                1440     625     218
 
BglII A'GATC_T

Cuts at:      0    1013    1908    2283 
   Size:       1013     895     375

  Fragments arranged by size: 

                1013     895     375
 
Bme1580I G_kGCm'C

Cuts at:      0     146     271    1807    1940    2283 
   Size:        146     125    1536     133     343

  Fragments arranged by size: 

                1536     343     146     133     125
 
BmgBI CAC'GTC

Cuts at:      0      44    2283 
   Size:         44    2239
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     842    2283 
   Size:        842    1441
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     996    1032    2283 
   Size:        996      36    1251

  Fragments arranged by size: 

                1251     996      36
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54      87     158    1093    1275    1335    1480    2116 
   Size:         54      33      71     935     182      60     145     636

Cuts at:   2116    2283 
   Size:        167

  Fragments arranged by size: 

                 935     636     182     167     145      71      60      54
                  33
 
Bpu10I CC'TnA_GC

Cuts at:      0     470    2283 
   Size:        470    1813
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     821    2100    2283 
   Size:        821    1279     183

  Fragments arranged by size: 

                1279     821     183
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     530    2283 
   Size:        530    1753
 
BsaBI GATnn'nnATC

Cuts at:      0    1907    2283 
   Size:       1907     376
 
BsaHI Gr'CG_yC

Cuts at:      0       4    2283 
   Size:          4    2279
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256     529     559    1555    1585    1636    1666 
   Size:        226      30     273      30     996      30      51      30

Cuts at:   1666    2106    2136    2283 
   Size:        440      30     147

  Fragments arranged by size: 

                 996     440     273     226     147      51      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     228     231     234     406     790     813     868    1024 
   Size:        228       3       3     172     384      23      55     156

Cuts at:   1024    1103    1414    1489    1660    2043    2283 
   Size:         79     311      75     171     383     240

  Fragments arranged by size: 

                 384     383     311     240     228     172     171     156
                  79      75      55      23       3       3
 
BseYI C'CCAG_C

Cuts at:      0     145     652    2283 
   Size:        145     507    1631

  Fragments arranged by size: 

                1631     507     145
 
BsiHKAI G_wGCw'C

Cuts at:      0     977    1807    2283 
   Size:        977     830     476

  Fragments arranged by size: 

                 977     830     476
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     146     217     271     547     860     977    1807 
   Size:         86      60      71      54     276     313     117     830

Cuts at:   1807    1940    1997    2283 
   Size:        133      57     286

  Fragments arranged by size: 

                 830     313     286     276     133     117      86      71
                  60      57      54
 
BspHI T'CATG_A

Cuts at:      0    1922    2283 
   Size:       1922     361
 
BsrBI CCG'CTC

Cuts at:      0      12    2283 
   Size:         12    2271
 
BsrDI GCAATG_nn'

Cuts at:      0    1962    2283 
   Size:       1962     321
 
BsrFI r'CCGG_y

Cuts at:      0     107    1972    2283 
   Size:        107    1865     311

  Fragments arranged by size: 

                1865     311     107
 
BsrGI T'GTAC_A

Cuts at:      0    1121    2283 
   Size:       1121    1162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1299    2283 
   Size:       1299     984
 
BstYI r'GATC_y

Cuts at:      0     340    1013    1662    1908    1952    2087    2283 
   Size:        340     673     649     246      44     135     196

  Fragments arranged by size: 

                 673     649     340     246     196     135      44
 
Bsu36I CC'TnA_GG

Cuts at:      0    1580    1757    2283 
   Size:       1580     177     526

  Fragments arranged by size: 

                1580     526     177
 
BtgI C'CryG_G

Cuts at:      0     657    2283 
   Size:        657    1626
 
BtsI GCAGTG_nn'

Cuts at:      0     728    1887    2045    2283 
   Size:        728    1159     158     238

  Fragments arranged by size: 

                1159     728     238     158
 
ClaI AT'CG_AT

Cuts at:      0     592    2283 
   Size:        592    1691
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     245     280    2283 
   Size:        245      35    2003

  Fragments arranged by size: 

                2003     245      35
 
EaeI y'GGCC_r

Cuts at:      0     109    2283 
   Size:        109    2174
 
EarI CTCTTCn'nnn_

Cuts at:      0     514     987    1222    2283 
   Size:        514     473     235    1061

  Fragments arranged by size: 

                1061     514     473     235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54      87     158     317     485     583    1046    1093 
   Size:         54      33      71     159     168      98     463      47

Cuts at:   1093    1275    1335    1480    2023    2116    2283 
   Size:        182      60     145     543      93     167

  Fragments arranged by size: 

                 543     463     182     168     167     159     145      98
                  93      71      60      54      47      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1421    1474    1642    2283 
   Size:       1421      53     168     641

  Fragments arranged by size: 

                1421     641     168      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142     268    1538    1676    2283 
   Size:        142     126    1270     138     607

  Fragments arranged by size: 

                1270     607     142     138     126
 
EcoRI G'AATT_C

Cuts at:      0    1329    2283 
   Size:       1329     954
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1893    2283 
   Size:       1893     390
 
HaeII r_GCGC'y

Cuts at:      0     162    2283 
   Size:        162    2121
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     529     561     877     909     910     942    2283 
   Size:        529      32     316      32       1      32    1341

  Fragments arranged by size: 

                1341     529     316      32      32      32       1
 
MfeI C'AATT_G

Cuts at:      0     488    2283 
   Size:        488    1795
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26    2283 
   Size:         26    2257
 
MslI CAynn'nnrTG

Cuts at:      0    2023    2038    2283 
   Size:       2023      15     245

  Fragments arranged by size: 

                2023     245      15
 
MspA1I CmG'CkG

Cuts at:      0      71     463    1848    1854    2283 
   Size:         71     392    1385       6     429

  Fragments arranged by size: 

                1385     429     392      71       6
 
NaeI GCC'GGC

Cuts at:      0     109    2283 
   Size:        109    2174
 
NcoI C'CATG_G

Cuts at:      0     657    2283 
   Size:        657    1626
 
NgoMIV G'CCGG_C

Cuts at:      0     107    2283 
   Size:        107    2176
 
NspI r_CATG'y

Cuts at:      0    1512    2022    2283 
   Size:       1512     510     261

  Fragments arranged by size: 

                1512     510     261
 
PciI A'CATG_T

Cuts at:      0    2018    2283 
   Size:       2018     265
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1154    2283 
   Size:       1154    1129
 
PfoI T'CCnGG_A

Cuts at:      0      77    2283 
   Size:         77    2206
 
PpuMI rG'GwC_Cy

Cuts at:      0    1538    2283 
   Size:       1538     745
 
PspOMI G'GGCC_C

Cuts at:      0     142     267    2283 
   Size:        142     125    2016

  Fragments arranged by size: 

                2016     142     125
 
PstI C_TGCA'G

Cuts at:      0     406     702    1853    2283 
   Size:        406     296    1151     430

  Fragments arranged by size: 

                1151     430     406     296
 
PvuII CAG'CTG

Cuts at:      0      71     463    1848    1854    2283 
   Size:         71     392    1385       6     429

  Fragments arranged by size: 

                1385     429     392      71       6
 
RsrII CG'GwC_CG

Cuts at:      0    2196    2283 
   Size:       2196      87
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1222    2283 
   Size:       1222    1061
 
ScaI AGT'ACT

Cuts at:      0    2008    2283 
   Size:       2008     275
 
SfcI C'TryA_G

Cuts at:      0     402     698    1268    1710    1849    2274    2283 
   Size:        402     296     570     442     139     425       9

  Fragments arranged by size: 

                 570     442     425     402     296     139       9
 
SmaI CCC'GGG

Cuts at:      0    2071    2177    2283 
   Size:       2071     106     106

  Fragments arranged by size: 

                2071     106     106
 
SmlI C'TyrA_G

Cuts at:      0     409     836    1746    2079    2283 
   Size:        409     427     910     333     204

  Fragments arranged by size: 

                 910     427     409     333     204
 
StuI AGG'CCT

Cuts at:      0    1306    2283 
   Size:       1306     977
 
StyI C'CwwG_G

Cuts at:      0     657     861    1132    1533    1877    2240    2283 
   Size:        657     204     271     401     344     363      43

  Fragments arranged by size: 

                 657     401     363     344     271     204      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     884    2283 
   Size:        884    1399
 
TatI w'GTAC_w

Cuts at:      0    1121    2006    2283 
   Size:       1121     885     277

  Fragments arranged by size: 

                1121     885     277
 
Tth111I GACn'n_nGTC

Cuts at:      0    1099    2283 
   Size:       1099    1184
 
XbaI T'CTAG_A

Cuts at:      0     387    2283 
   Size:        387    1896
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     366    2022    2283 
   Size:        366    1656     261

  Fragments arranged by size: 

                1656     366     261
 
XmaI C'CCGG_G

Cuts at:      0    2069    2175    2283 
   Size:       2069     106     108

  Fragments arranged by size: 

                2069     108     106
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI    CspCI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I     MfeI     MmeI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII    RsrII     SapI     ScaI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TatI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI    BsaAI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI
     XhoI     XmnI     ZraI
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