Restriction map for pF1KSDA0215
 (Linear) (Six-base) MAPSORT of: KIAA0215.seq  Check: 2954  from: 1  to: 2469



KIAA0215.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1728    2469 
   Size:       1728     741
 
AccI GT'mk_AC

Cuts at:      0    2244    2469 
   Size:       2244     225
 
Acc65I G'GTAC_C

Cuts at:      0     243    2469 
   Size:        243    2226
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     814    1327    1504    1909    2469 
   Size:        427     387     513     177     405     560

  Fragments arranged by size: 

                 560     513     427     405     387     177
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     420    2469 
   Size:        420    2049
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1201    1233    2062    2094    2469 
   Size:       1201      32     829      32     375

  Fragments arranged by size: 

                1201     829     375      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     367     400    1434    1905    2469 
   Size:        367      33    1034     471     564

  Fragments arranged by size: 

                1034     564     471     367      33
 
ApoI r'AATT_y

Cuts at:      0      95    2469 
   Size:         95    2374
 
AseI AT'TA_AT

Cuts at:      0     179    2469 
   Size:        179    2290
 
AvaI C'yCGr_G

Cuts at:      0    2046    2469 
   Size:       2046     423
 
BamHI G'GATC_C

Cuts at:      0     845    2469 
   Size:        845    1624
 
BanI G'GyrC_C

Cuts at:      0     243    1740    2469 
   Size:        243    1497     729

  Fragments arranged by size: 

                1497     729     243
 
BanII G_rGCy'C

Cuts at:      0     792     825    2224    2277    2469 
   Size:        792      33    1399      53     192

  Fragments arranged by size: 

                1399     792     192      53      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464     802     958    1045    1658    1940    2469 
   Size:        464     338     156      87     613     282     529

  Fragments arranged by size: 

                 613     529     464     338     282     156      87
 
BbvCI CC'TCA_GC

Cuts at:      0     122    2469 
   Size:        122    2347
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1436    1470    2469 
   Size:       1436      34     999

  Fragments arranged by size: 

                1436     999      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1787    2469 
   Size:       1787     682
 
BfrBI ATG'CAT

Cuts at:      0    1419    2469 
   Size:       1419    1050
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2009    2469 
   Size:       2009     460
 
BglII A'GATC_T

Cuts at:      0     896    1514    2469 
   Size:        896     618     955

  Fragments arranged by size: 

                 955     896     618
 
Bme1580I G_kGCm'C

Cuts at:      0     840    1191    2469 
   Size:        840     351    1278

  Fragments arranged by size: 

                1278     840     351
 
BmgBI CAC'GTC

Cuts at:      0    1005    2469 
   Size:       1005    1464
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     908    1915    2469 
   Size:        908    1007     554

  Fragments arranged by size: 

                1007     908     554
 
BmtI G_CTAG'C

Cuts at:      0    1276    2469 
   Size:       1276    1193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     608    1228    1456    2026    2173    2469 
   Size:        330     278     620     228     570     147     296

  Fragments arranged by size: 

                 620     570     330     296     278     228     147
 
Bpu10I CC'TnA_GC

Cuts at:      0     122    2469 
   Size:        122    2347
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688    1072    2469 
   Size:        688     384    1397

  Fragments arranged by size: 

                1397     688     384
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     626    2146    2469 
   Size:        626    1520     323

  Fragments arranged by size: 

                1520     626     323
 
BsaBI GATnn'nnATC

Cuts at:      0     494    2469 
   Size:        494    1975
 
BsaWI w'CCGG_w

Cuts at:      0     131    2469 
   Size:        131    2338
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     227     257    1049    1079    1201    1231    2062    2092 
   Size:        227      30     792      30     122      30     831      30

Cuts at:   2092    2469 
   Size:        377

  Fragments arranged by size: 

                 831     792     377     227     122      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1225    1228    2469 
   Size:       1225       3    1241

  Fragments arranged by size: 

                1241    1225       3
 
BseYI C'CCAG_C

Cuts at:      0    1257    2469 
   Size:       1257    1212
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1393    2469 
   Size:       1393    1076
 
BsiHKAI G_wGCw'C

Cuts at:      0     976    1025    2277    2469 
   Size:        976      49    1252     192

  Fragments arranged by size: 

                1252     976     192      49
 
BsmI GAATG_Cn'

Cuts at:      0    1391    2469 
   Size:       1391    1078
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1052    2469 
   Size:       1052    1417
 
Bsp1286I G_dGCh'C

Cuts at:      0     792     825     840     976    1025    1191    2224    2277 
   Size:        792      33      15     136      49     166    1033      53

Cuts at:   2277    2469 
   Size:        192

  Fragments arranged by size: 

                1033     792     192     166     136      53      49      33
                  15
 
BspEI T'CCGG_A

Cuts at:      0     131    2469 
   Size:        131    2338
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     126    1728    2469 
   Size:        126    1602     741

  Fragments arranged by size: 

                1602     741     126
 
BsrDI GCAATG_nn'

Cuts at:      0     531     766     860    2469 
   Size:        531     235      94    1609

  Fragments arranged by size: 

                1609     531     235      94
 
BsrFI r'CCGG_y

Cuts at:      0     617    2469 
   Size:        617    1852
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     941    2469 
   Size:        941    1528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2004    2469 
   Size:       2004     465
 
BstYI r'GATC_y

Cuts at:      0     845     896    1514    2469 
   Size:        845      51     618     955

  Fragments arranged by size: 

                 955     845     618      51
 
Bsu36I CC'TnA_GG

Cuts at:      0    1745    2469 
   Size:       1745     724
 
BtgI C'CryG_G

Cuts at:      0    1938    2421    2469 
   Size:       1938     483      48

  Fragments arranged by size: 

                1938     483      48
 
BtsI GCAGTG_nn'

Cuts at:      0      33     339     992    2469 
   Size:         33     306     653    1477

  Fragments arranged by size: 

                1477     653     306      33
 
DraI TTT'AAA

Cuts at:      0    2148    2469 
   Size:       2148     321
 
EaeI y'GGCC_r

Cuts at:      0    1247    1817    2469 
   Size:       1247     570     652

  Fragments arranged by size: 

                1247     652     570
 
EcoICRI GAG'CTC

Cuts at:      0    2275    2469 
   Size:       2275     194
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     427     608     814    1228    1327    1456    1504 
   Size:        330      97     181     206     414      99     129      48

Cuts at:   1504    1909    2026    2173    2469 
   Size:        405     117     147     296

  Fragments arranged by size: 

                 414     405     330     296     206     181     147     129
                 117      99      97      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1051    2469 
   Size:       1051    1418
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138     708    1431    2141    2184    2469 
   Size:        138     570     723     710      43     285

  Fragments arranged by size: 

                 723     710     570     285     138      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1552    2469 
   Size:       1552     917
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1693    1725    2237    2269    2469 
   Size:       1693      32     512      32     200

  Fragments arranged by size: 

                1693     512     200      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1195    2469 
   Size:       1195    1274
 
KpnI G_GTAC'C

Cuts at:      0     247    2469 
   Size:        247    2222
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     345    2469 
   Size:        345    2124
 
MscI TGG'CCA

Cuts at:      0    1819    2469 
   Size:       1819     650
 
MslI CAynn'nnrTG

Cuts at:      0     536    1402    1738    2469 
   Size:        536     866     336     731

  Fragments arranged by size: 

                 866     731     536     336
 
MspA1I CmG'CkG

Cuts at:      0     722     833    2015    2469 
   Size:        722     111    1182     454

  Fragments arranged by size: 

                1182     722     454     111
 
NcoI C'CATG_G

Cuts at:      0    1938    2421    2469 
   Size:       1938     483      48

  Fragments arranged by size: 

                1938     483      48
 
NheI G'CTAG_C

Cuts at:      0    1272    2469 
   Size:       1272    1197
 
NsiI A_TGCA'T

Cuts at:      0    1421    2469 
   Size:       1421    1048
 
NspI r_CATG'y

Cuts at:      0    1737    2469 
   Size:       1737     732
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     920    1434    2165    2469 
   Size:        920     514     731     304

  Fragments arranged by size: 

                 920     731     514     304
 
PfoI T'CCnGG_A

Cuts at:      0     521    2469 
   Size:        521    1948
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1201    1233    2062    2094    2469 
   Size:       1201      32     829      32     375

  Fragments arranged by size: 

                1201     829     375      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     138     708    1431    2141    2184    2469 
   Size:        138     570     723     710      43     285

  Fragments arranged by size: 

                 723     710     570     285     138      43
 
PshAI GACnn'nnGTC

Cuts at:      0      44    2469 
   Size:         44    2425
 
PspXI vC'TCGA_Gb

Cuts at:      0    2046    2469 
   Size:       2046     423
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     293     325    2469 
   Size:        293      32    2144

  Fragments arranged by size: 

                2144     293      32
 
PstI C_TGCA'G

Cuts at:      0    2268    2469 
   Size:       2268     201
 
PvuII CAG'CTG

Cuts at:      0     722     833    2015    2469 
   Size:        722     111    1182     454

  Fragments arranged by size: 

                1182     722     454     111
 
SacI G_AGCT'C

Cuts at:      0    2277    2469 
   Size:       2277     192
 
SfcI C'TryA_G

Cuts at:      0    2123    2264    2469 
   Size:       2123     141     205

  Fragments arranged by size: 

                2123     205     141
 
SmlI C'TyrA_G

Cuts at:      0     703    1087    2046    2469 
   Size:        703     384     959     423

  Fragments arranged by size: 

                 959     703     423     384
 
StuI AGG'CCT

Cuts at:      0      15     688    2469 
   Size:         15     673    1781

  Fragments arranged by size: 

                1781     673      15
 
StyI C'CwwG_G

Cuts at:      0    1226    1938    2225    2421    2469 
   Size:       1226     712     287     196      48

  Fragments arranged by size: 

                1226     712     287     196      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     927    2047    2157    2469 
   Size:        927    1120     110     312

  Fragments arranged by size: 

                1120     927     312     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1049    1081    2176    2208    2469 
   Size:       1049      32    1095      32     261

  Fragments arranged by size: 

                1095    1049     261      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2407    2469 
   Size:       2407      62
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     719     917    1275    2469 
   Size:        719     198     358    1194

  Fragments arranged by size: 

                1194     719     358     198
 
XhoI C'TCGA_G

Cuts at:      0    2046    2469 
   Size:       2046     423
 
XmnI GAAnn'nnTTC

Cuts at:      0    1391    2469 
   Size:       1391    1078
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AlfI     AloI    AlwNI     ApoI
     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     DraI     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NheI
     NsiI     NspI    PflMI     PfoI     PpiI    PpuMI    PshAI    PspXI
     PsrI     PstI    PvuII     SacI     SfcI     SmlI     StuI     StyI
    TaqII     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI     BbeI     BclI
     BlpI     BplI    BsaAI    BsaHI    BsiEI    BsiWI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI
     PasI     PciI     PmeI     PmlI     PsiI   PspOMI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XmaI     ZraI
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