Restriction map for pF1KSDA0207
 (Linear) (Six-base) MAPSORT of: KIAA0207.seq  Check: 3164  from: 1  to: 1764



KIAA0207.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     986    1764 
   Size:        986     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     334    1629    1764 
   Size:        242      92    1295     135

  Fragments arranged by size: 

                1295     242     135      92
 
AfeI AGC'GCT

Cuts at:      0     313    1764 
   Size:        313    1451
 
AflIII A'CryG_T

Cuts at:      0    1308    1764 
   Size:       1308     456
 
AlwNI CAG_nnn'CTG

Cuts at:      0     987    1764 
   Size:        987     777
 
ApaLI G'TGCA_C

Cuts at:      0     325    1764 
   Size:        325    1439
 
ApoI r'AATT_y

Cuts at:      0     706     759     828     857    1602    1669    1764 
   Size:        706      53      69      29     745      67      95

  Fragments arranged by size: 

                 745     706      95      69      67      53      29
 
AvaI C'yCGr_G

Cuts at:      0     251     294    1764 
   Size:        251      43    1470

  Fragments arranged by size: 

                1470     251      43
 
AvrII C'CTAG_G

Cuts at:      0     633    1395    1764 
   Size:        633     762     369

  Fragments arranged by size: 

                 762     633     369
 
BanI G'GyrC_C

Cuts at:      0     210     344     788    1764 
   Size:        210     134     444     976

  Fragments arranged by size: 

                 976     444     210     134
 
BanII G_rGCy'C

Cuts at:      0     433    1067    1517    1764 
   Size:        433     634     450     247

  Fragments arranged by size: 

                 634     450     433     247
 
BbeI G_GCGC'C

Cuts at:      0     348    1764 
   Size:        348    1416
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     365     662    1764 
   Size:        365     297    1102

  Fragments arranged by size: 

                1102     365     297
 
BbvCI CC'TCA_GC

Cuts at:      0    1108    1211    1764 
   Size:       1108     103     553

  Fragments arranged by size: 

                1108     553     103
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      34      68     142     176    1233    1267    1764 
   Size:         34      34      74      34    1057      34     497

  Fragments arranged by size: 

                1057     497      74      34      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0     163    1764 
   Size:        163    1601
 
BglII A'GATC_T

Cuts at:      0     928    1610    1764 
   Size:        928     682     154

  Fragments arranged by size: 

                 928     682     154
 
Bme1580I G_kGCm'C

Cuts at:      0     215     329    1764 
   Size:        215     114    1435

  Fragments arranged by size: 

                1435     215     114
 
BmgBI CAC'GTC

Cuts at:      0    1145    1764 
   Size:       1145     619
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     825    1018    1369    1386    1461    1764 
   Size:        190     635     193     351      17      75     303

  Fragments arranged by size: 

                 635     351     303     193     190      75      17
 
Bpu10I CC'TnA_GC

Cuts at:      0    1108    1211    1764 
   Size:       1108     103     553

  Fragments arranged by size: 

                1108     553     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1156    1720    1764 
   Size:       1156     564      44

  Fragments arranged by size: 

                1156     564      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     548    1764 
   Size:        548    1216
 
BsaBI GATnn'nnATC

Cuts at:      0    1390    1764 
   Size:       1390     374
 
BsaHI Gr'CG_yC

Cuts at:      0     345    1246    1764 
   Size:        345     901     518

  Fragments arranged by size: 

                 901     518     345
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      68      98     163     193    1000    1030    1764 
   Size:         68      30      65      30     807      30     734

  Fragments arranged by size: 

                 807     734      68      65      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     257    1159    1366    1525    1764 
   Size:        257     902     207     159     239

  Fragments arranged by size: 

                 902     257     239     207     159
 
BseYI C'CCAG_C

Cuts at:      0     317     815     885    1764 
   Size:        317     498      70     879

  Fragments arranged by size: 

                 879     498     317      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328    1142    1764 
   Size:        328     814     622

  Fragments arranged by size: 

                 814     622     328
 
BsiHKAI G_wGCw'C

Cuts at:      0     329     443     623    1764 
   Size:        329     114     180    1141

  Fragments arranged by size: 

                1141     329     180     114
 
BsmI GAATG_Cn'

Cuts at:      0     165    1187    1764 
   Size:        165    1022     577

  Fragments arranged by size: 

                1022     577     165
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     329     433     443     623    1067    1517    1764 
   Size:        215     114     104      10     180     444     450     247

  Fragments arranged by size: 

                 450     444     247     215     180     114     104      10
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     986    1764 
   Size:        986     778
 
BsrDI GCAATG_nn'

Cuts at:      0    1289    1764 
   Size:       1289     475
 
BsrFI r'CCGG_y

Cuts at:      0      10     387    1764 
   Size:         10     377    1377

  Fragments arranged by size: 

                1377     377      10
 
BssSI C'ACGA_G

Cuts at:      0    1276    1516    1764 
   Size:       1276     240     248

  Fragments arranged by size: 

                1276     248     240
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     306    1764 
   Size:        306    1458
 
BstYI r'GATC_y

Cuts at:      0     928    1472    1610    1764 
   Size:        928     544     138     154

  Fragments arranged by size: 

                 928     544     154     138
 
BtgI C'CryG_G

Cuts at:      0     689    1059    1764 
   Size:        689     370     705

  Fragments arranged by size: 

                 705     689     370
 
BtsI GCAGTG_nn'

Cuts at:      0    1263    1743    1764 
   Size:       1263     480      21

  Fragments arranged by size: 

                1263     480      21
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     503     538    1764 
   Size:        503      35    1226

  Fragments arranged by size: 

                1226     503      35
 
DraI TTT'AAA

Cuts at:      0     747    1764 
   Size:        747    1017
 
DraIII CAC_nnn'GTG

Cuts at:      0     591    1580    1764 
   Size:        591     989     184

  Fragments arranged by size: 

                 989     591     184
 
EaeI y'GGCC_r

Cuts at:      0     228     389     692     989    1764 
   Size:        228     161     303     297     775

  Fragments arranged by size: 

                 775     303     297     228     161
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1342    1764 
   Size:       1342     422
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     242     334     825    1018    1369    1386    1461 
   Size:        190      52      92     491     193     351      17      75

Cuts at:   1461    1629    1764 
   Size:        168     135

  Fragments arranged by size: 

                 491     351     193     190     168     135      92      75
                  52      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352    1351    1764 
   Size:        352     999     413

  Fragments arranged by size: 

                 999     413     352
 
EcoO109I rG'GnC_Cy

Cuts at:      0     262    1764 
   Size:        262    1502
 
FspI TGC'GCA

Cuts at:      0    1255    1764 
   Size:       1255     509
 
HaeII r_GCGC'y

Cuts at:      0     315     348    1764 
   Size:        315      33    1416

  Fragments arranged by size: 

                1416     315      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      68     100    1305    1337    1764 
   Size:         68      32    1205      32     427

  Fragments arranged by size: 

                1205     427      68      32      32
 
HincII GTy'rAC

Cuts at:      0      61    1695    1764 
   Size:         61    1634      69

  Fragments arranged by size: 

                1634      69      61
 
KasI G'GCGC_C

Cuts at:      0     344    1764 
   Size:        344    1420
 
MfeI C'AATT_G

Cuts at:      0     565    1764 
   Size:        565    1199
 
MluI A'CGCG_T

Cuts at:      0    1308    1764 
   Size:       1308     456
 
MscI TGG'CCA

Cuts at:      0     230     694    1764 
   Size:        230     464    1070

  Fragments arranged by size: 

                1070     464     230
 
MslI CAynn'nnrTG

Cuts at:      0    1384    1753    1764 
   Size:       1384     369      11

  Fragments arranged by size: 

                1384     369      11
 
MspA1I CmG'CkG

Cuts at:      0     436     605     984    1689    1707    1764 
   Size:        436     169     379     705      18      57

  Fragments arranged by size: 

                 705     436     379     169      57      18
 
NaeI GCC'GGC

Cuts at:      0      12     389    1764 
   Size:         12     377    1375

  Fragments arranged by size: 

                1375     377      12
 
NarI GG'CG_CC

Cuts at:      0     345    1764 
   Size:        345    1419
 
NcoI C'CATG_G

Cuts at:      0     689    1764 
   Size:        689    1075
 
NgoMIV G'CCGG_C

Cuts at:      0      10     387    1764 
   Size:         10     377    1377

  Fragments arranged by size: 

                1377     377      10
 
NsiI A_TGCA'T

Cuts at:      0     165    1764 
   Size:        165    1599
 
NspI r_CATG'y

Cuts at:      0     198     238     877    1764 
   Size:        198      40     639     887

  Fragments arranged by size: 

                 887     639     198      40
 
PasI CC'CwG_GG

Cuts at:      0     424    1764 
   Size:        424    1340
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     570     666    1764 
   Size:        570      96    1098

  Fragments arranged by size: 

                1098     570      96
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398    1764 
   Size:        366      32    1366

  Fragments arranged by size: 

                1366     366      32
 
PstI C_TGCA'G

Cuts at:      0     193     224     983    1764 
   Size:        193      31     759     781

  Fragments arranged by size: 

                 781     759     193      31
 
PvuII CAG'CTG

Cuts at:      0     605     984    1689    1707    1764 
   Size:        605     379     705      18      57

  Fragments arranged by size: 

                 705     605     379      57      18
 
SfcI C'TryA_G

Cuts at:      0     189     220     979    1052    1764 
   Size:        189      31     759      73     712

  Fragments arranged by size: 

                 759     712     189      73      31
 
SfoI GGC'GCC

Cuts at:      0     346    1764 
   Size:        346    1418
 
SmlI C'TyrA_G

Cuts at:      0    1171    1735    1764 
   Size:       1171     564      29

  Fragments arranged by size: 

                1171     564      29
 
SpeI A'CTAG_T

Cuts at:      0     612    1764 
   Size:        612    1152
 
SphI G_CATG'C

Cuts at:      0     198     238    1764 
   Size:        198      40    1526

  Fragments arranged by size: 

                1526     198      40
 
StuI AGG'CCT

Cuts at:      0    1225    1764 
   Size:       1225     539
 
StyI C'CwwG_G

Cuts at:      0     633     689     950    1395    1764 
   Size:        633      56     261     445     369

  Fragments arranged by size: 

                 633     445     369     261      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     116     260    1764 
   Size:        116     144    1504

  Fragments arranged by size: 

                1504     144     116
 
TatI w'GTAC_w

Cuts at:      0     179    1040    1432    1569    1764 
   Size:        179     861     392     137     195

  Fragments arranged by size: 

                 861     392     195     179     137
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     799    1764 
   Size:        799     965
 
XmnI GAAnn'nnTTC

Cuts at:      0     763    1764 
   Size:        763    1001
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    AlwNI    ApaLI     ApoI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI
    BglII Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstYI     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    HaeII    Hin4I   HincII     KasI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
     PasI    PflMI     PpiI     PstI    PvuII     SfcI     SfoI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI     BaeI    BamHI
    BciVI     BclI     BglI     BlpI     BmrI     BmtI     BplI    BsaAI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI  HindIII     HpaI     KpnI     MmeI     NdeI     NheI     NotI
     NruI     PacI     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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