Restriction map for pF1KSDA0190
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AvrII       BbeI        BbrPI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspEI       BspLU11I    
BsrBI       BsrDI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     CpoI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoT22I     FseI        HaeII       
HgaI        KasI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        RsrII       SacI        
SacII       SbfI        ScaI        SfiI        SfoI        
SfuI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AatII        2272  GACGTC               GACGT^C
Acc65I        838  GGTACC               G^GTACC
AcyI         2272  GACGTC               G[GA]^CG[CT]C
AhdI          799  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          268  CCTCAGG              CC^T[ACGT]AGG
AseI         1291  ATTAAT               AT^TAAT
AsnI         1291  ATTAAT               AT^TAAT
Asp718I       838  GGTACC               G^GTACC
AspEI         799  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         704  GAGCAC               G[AT]GC[AT]^C
AspI          664  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2303  TGCGCA               TGC^GCA
BalI          549  TGGCCA               TGG^CCA
BamHI        1182  GGATCC               G^GATCC
BbvCI        1585  GCTGAGG              CCTCAGC(-5/-2)
BlpI          823  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      823  GCTCAGC              GC^T[ACGT]AGC
BsaHI        2272  GACGTC               G[GA]^CG[CT]C
BsaWI        1170  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       704  GAGCAC               G[AT]GC[AT]^C
BsmFI         568  GTCCC                GGGAC(10/14)
Bsp1407I      295  TGTACA               T^GTACA
BspDI        2308  ATCGAT               AT^CGAT
BsrGI         295  TGTACA               T^GTACA
BssSI        2059  CTCGTG               CACGAG(-5/-1)
BstXI         911  CCAACGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1182  GGATCC               [GA]^GATC[CT]
Bsu36I        268  CCTCAGG              CC^T[ACGT]AGG
CelII         823  GCTCAGC              GC^T[ACGT]AGC
CfoI         2304  GCGC                 GCG^C
ClaI         2308  ATCGAT               AT^CGAT
DraI         2191  TTTAAA               TTT^AAA
DrdI         2314  GACCAGACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      799  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        268  CCTCAGG              CC^T[ACGT]AGG
EcoRI          64  GAATTC               G^AATTC
EcoRV        1915  GATATC               GAT^ATC
FspI         2303  TGCGCA               TGC^GCA
HhaI         2304  GCGC                 GCG^C
Hin1I        2272  GACGTC               G[GA]^CG[CT]C
HinP1I       2304  GCGC                 G^CGC
HpaI         1543  GTTAAC               GTT^AAC
KpnI          838  GGTACC               GGTAC^C
MflI         1182  GGATCC               [GA]^GATC[CT]
MluNI         549  TGGCCA               TGG^CCA
MscI          549  TGGCCA               TGG^CCA
MslI         1431  CACTAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        815  CAGCTG               C[AC]G^C[GT]G
NcoI           14  CCATGG               C^CATGG
NspI          611  ACATGC               [GA]CATG^[CT]
PflFI         664  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1779  GACTTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1291  ATTAAT               AT^TAAT
PvuII         815  CAGCTG               CAG^CTG
SalI         1571  GTCGAC               G^TCGAC
SapI         1714  GAAGAGC              GCTCTTC(1/4)
SexAI        2342  ACCAGGT              A^CC[AT]GGT
SgrAI        1079  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI          751  CCCGGG               CCC^GGG
SspBI         295  TGTACA               T^GTACA
SspI         2122  AATATT               AAT^ATT
StuI         1375  AGGCCT               AGG^CCT
Tth111I       664  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1182  GGATCC               [GA]^GATC[CT]
XmaCI         751  CCCGGG               C^CCGGG
XmaI          751  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1571  GTCGAC               GT^[AC][GT]AC
             2227  GTCTAC              
AlwI         1183  GATCC                GGATC(4/5)
             1182  GGATC               
ApaI          747  GGGCCC               GGGCC^C
             1764  GGGCCC              
AvaII         951  GGACC                G^G[AT]CC
             2400  GGACC               
BbsI         2091  GAAGAC               GAAGAC(2/6)
             2275  GTCTTC              
BclI         1265  TGATCA               T^GATCA
             2330  TGATCA              
BpuAI        2091  GAAGAC               GAAGAC(2/6)
             2275  GTCTTC              
BseRI          86  CTCCTC               GAGGAG(10/8)
             1074  CTCCTC              
BsgI         2065  CTGCAC               GTGCAG(16/14)
              584  GTGCAG              
BsmAI         797  GAGAC                GTCTC(1/5)
              433  GTCTC               
BspHI        1053  TCATGA               T^CATGA
             1620  TCATGA              
BspMI        2402  ACCTGC               ACCTGC(4/8)
             1143  GCAGGT              
BsrFI        1080  GCCGGT               [GA]^CCGG[CT]
             1822  ACCGGC              
BstAPI        586  GCAGAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1309  GCATTGGTTGC         
Cfr10I       1080  GCCGGT               [GA]^CCGG[CT]
             1822  ACCGGC              
FbaI         1265  TGATCA               T^GATCA
             2330  TGATCA              
HindIII      1645  AAGCTT               A^AGCTT
             2110  AAGCTT              
PflMI         112  CCATACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1031  CCAAGTCCTGG         
RcaI         1053  TCATGA               T^CATGA
             1620  TCATGA              
Van91I        112  CCATACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1031  CCAAGTCCTGG         
XcmI          530  CCACAGAAGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1118  CCATATCTCCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        Asp700I     AvaI        BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
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