Restriction map for pF1KSDA0190
 (Linear) (Six-base) MAPSORT of: KIAA0190.seq  Check: 9286  from: 1  to: 2409



KIAA0190.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AatII G_ACGT'C

Cuts at:      0    2276    2409 
   Size:       2276     133
 
AccI GT'mk_AC

Cuts at:      0    1572    2228    2409 
   Size:       1572     656     181

  Fragments arranged by size: 

                1572     656     181
 
Acc65I G'GTAC_C

Cuts at:      0     838    2409 
   Size:        838    1571
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    2409 
   Size:         80    2329
 
AhdI GACnn_n'nnGTC

Cuts at:      0     804    2409 
   Size:        804    1605
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1765    1797    2409 
   Size:       1765      32     612

  Fragments arranged by size: 

                1765     612      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     373     667     790    2409 
   Size:        373     294     123    1619

  Fragments arranged by size: 

                1619     373     294     123
 
ApaI G_GGCC'C

Cuts at:      0     751    1768    2409 
   Size:        751    1017     641

  Fragments arranged by size: 

                1017     751     641
 
ApoI r'AATT_y

Cuts at:      0      64     280     598    2187    2409 
   Size:         64     216     318    1589     222

  Fragments arranged by size: 

                1589     318     222     216      64
 
AseI AT'TA_AT

Cuts at:      0    1292    2409 
   Size:       1292    1117
 
AvaI C'yCGr_G

Cuts at:      0     751     891     964    2409 
   Size:        751     140      73    1445

  Fragments arranged by size: 

                1445     751     140      73
 
BamHI G'GATC_C

Cuts at:      0    1182    2409 
   Size:       1182    1227
 
BanI G'GyrC_C

Cuts at:      0     838    1009    2102    2409 
   Size:        838     171    1093     307

  Fragments arranged by size: 

                1093     838     307     171
 
BanII G_rGCy'C

Cuts at:      0     751     825    1000    1517    1768    2409 
   Size:        751      74     175     517     251     641

  Fragments arranged by size: 

                 751     641     517     251     175      74
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2098    2268    2409 
   Size:       2098     170     141

  Fragments arranged by size: 

                2098     170     141
 
BbvCI CC'TCA_GC

Cuts at:      0    1586    2409 
   Size:       1586     823
 
BclI T'GATC_A

Cuts at:      0    1265    2330    2409 
   Size:       1265    1065      79

  Fragments arranged by size: 

                1265    1065      79
 
BlpI GC'TnA_GC

Cuts at:      0     824    2409 
   Size:        824    1585
 
Bme1580I G_kGCm'C

Cuts at:      0     751     904    1768    2409 
   Size:        751     153     864     641

  Fragments arranged by size: 

                 864     751     641     153
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     209    2409 
   Size:        209    2200
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1234    1520    1975    2153    2409 
   Size:       1234     286     455     178     256

  Fragments arranged by size: 

                1234     455     286     256     178
 
Bpu10I CC'TnA_GC

Cuts at:      0    1586    2409 
   Size:       1586     823
 
BsaHI Gr'CG_yC

Cuts at:      0    2273    2409 
   Size:       2273     136
 
BsaWI w'CCGG_w

Cuts at:      0    1170    2409 
   Size:       1170    1239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77    1065    2409 
   Size:         77     988    1344

  Fragments arranged by size: 

                1344     988      77
 
BseYI C'CCAG_C

Cuts at:      0     238     817    2409 
   Size:        238     579    1592

  Fragments arranged by size: 

                1592     579     238
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    2050    2409 
   Size:        605    1445     359

  Fragments arranged by size: 

                1445     605     359
 
BsiHKAI G_wGCw'C

Cuts at:      0     708    2409 
   Size:        708    1701
 
Bsp1286I G_dGCh'C

Cuts at:      0     708     751     825     904    1000    1517    1768    2409 
   Size:        708      43      74      79      96     517     251     641

  Fragments arranged by size: 

                 708     641     517     251      96      79      74      43
            
 
BspHI T'CATG_A

Cuts at:      0    1053    1620    2409 
   Size:       1053     567     789

  Fragments arranged by size: 

                1053     789     567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1134    2409 
   Size:       1134    1275
 
BsrFI r'CCGG_y

Cuts at:      0    1080    1822    2409 
   Size:       1080     742     587

  Fragments arranged by size: 

                1080     742     587
 
BsrGI T'GTAC_A

Cuts at:      0     295    2409 
   Size:        295    2114
 
BssSI C'ACGA_G

Cuts at:      0    2059    2409 
   Size:       2059     350
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     592    1315    2409 
   Size:        592     723    1094

  Fragments arranged by size: 

                1094     723     592
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     918    2409 
   Size:        918    1491
 
BstYI r'GATC_y

Cuts at:      0    1182    2409 
   Size:       1182    1227
 
Bsu36I CC'TnA_GG

Cuts at:      0     269    2409 
   Size:        269    2140
 
BtgI C'CryG_G

Cuts at:      0      14     932    1257    2409 
   Size:         14     918     325    1152

  Fragments arranged by size: 

                1152     918     325      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     530    2409 
   Size:        530    1879
 
BtsI GCAGTG_nn'

Cuts at:      0     720    1300    2228    2409 
   Size:        720     580     928     181

  Fragments arranged by size: 

                 928     720     580     181
 
ClaI AT'CG_AT

Cuts at:      0    2309    2409 
   Size:       2309     100
 
DraI TTT'AAA

Cuts at:      0    2193    2409 
   Size:       2193     216
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2320    2409 
   Size:       2320      89
 
EaeI y'GGCC_r

Cuts at:      0     478     549    2256    2409 
   Size:        478      71    1707     153

  Fragments arranged by size: 

                1707     478     153      71
 
EarI CTCTTCn'nnn_

Cuts at:      0    1074    1709    2022    2409 
   Size:       1074     635     313     387

  Fragments arranged by size: 

                1074     635     387     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     424    1813    2409 
   Size:        424    1389     596

  Fragments arranged by size: 

                1389     596     424
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1234    1520    1975    2153    2409 
   Size:         80    1154     286     455     178     256

  Fragments arranged by size: 

                1154     455     286     256     178      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     273     747     748    1765    2409 
   Size:        273     474       1    1017     644

  Fragments arranged by size: 

                1017     644     474     273       1
 
EcoRI G'AATT_C

Cuts at:      0      64    2409 
   Size:         64    2345
 
EcoRV GAT'ATC

Cuts at:      0    1917    2409 
   Size:       1917     492
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1180    2179    2409 
   Size:       1180     999     230

  Fragments arranged by size: 

                1180     999     230
 
FspI TGC'GCA

Cuts at:      0    2305    2409 
   Size:       2305     104
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     418     450     511     543     790     822     958     990 
   Size:        418      32      61      32     247      32     136      32

Cuts at:    990    1463    1495    1906    1938    1964    1996    2409 
   Size:        473      32     411      32      26      32     413

  Fragments arranged by size: 

                 473     418     413     411     247     136      61      32
                  32      32      32      32      32      32      26
 
HincII GTy'rAC

Cuts at:      0    1388    1545    1573    2409 
   Size:       1388     157      28     836

  Fragments arranged by size: 

                1388     836     157      28
 
HindIII A'AGCT_T

Cuts at:      0    1645    2110    2409 
   Size:       1645     465     299

  Fragments arranged by size: 

                1645     465     299
 
HpaI GTT'AAC

Cuts at:      0    1545    2409 
   Size:       1545     864
 
KpnI G_GTAC'C

Cuts at:      0     842    2409 
   Size:        842    1567
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1706    1892    2409 
   Size:       1706     186     517

  Fragments arranged by size: 

                1706     517     186
 
MscI TGG'CCA

Cuts at:      0     551    2409 
   Size:        551    1858
 
MslI CAynn'nnrTG

Cuts at:      0    1435    2409 
   Size:       1435     974
 
MspA1I CmG'CkG

Cuts at:      0     817    2409 
   Size:        817    1592
 
NcoI C'CATG_G

Cuts at:      0      14    2409 
   Size:         14    2395
 
NspI r_CATG'y

Cuts at:      0     615    2409 
   Size:        615    1794
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     118    1037    2409 
   Size:        118     919    1372

  Fragments arranged by size: 

                1372     919     118
 
PfoI T'CCnGG_A

Cuts at:      0    1943    2409 
   Size:       1943     466
 
PshAI GACnn'nnGTC

Cuts at:      0    1783    2409 
   Size:       1783     626
 
PspOMI G'GGCC_C

Cuts at:      0     747    1764    2409 
   Size:        747    1017     645

  Fragments arranged by size: 

                1017     747     645
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1270    1302    2409 
   Size:       1270      32    1107

  Fragments arranged by size: 

                1270    1107      32
 
PstI C_TGCA'G

Cuts at:      0     732     786    1307    2409 
   Size:        732      54     521    1102

  Fragments arranged by size: 

                1102     732     521      54
 
PvuII CAG'CTG

Cuts at:      0     817    2409 
   Size:        817    1592
 
SalI G'TCGA_C

Cuts at:      0    1571    2409 
   Size:       1571     838
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1709    2409 
   Size:       1709     700
 
SexAI A'CCwGG_T

Cuts at:      0    2342    2409 
   Size:       2342      67
 
SfcI C'TryA_G

Cuts at:      0     243     728     782    1187    1303    2267    2409 
   Size:        243     485      54     405     116     964     142

  Fragments arranged by size: 

                 964     485     405     243     142     116      54
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1080    2409 
   Size:       1080    1329
 
SmaI CCC'GGG

Cuts at:      0     753    2409 
   Size:        753    1656
 
SspI AAT'ATT

Cuts at:      0    2124    2409 
   Size:       2124     285
 
StuI AGG'CCT

Cuts at:      0    1377    2409 
   Size:       1377    1032
 
StyI C'CwwG_G

Cuts at:      0      14     858    1983    2409 
   Size:         14     844    1125     426

  Fragments arranged by size: 

                1125     844     426      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     595    1407    1692    2090    2275    2409 
   Size:        595     812     285     398     185     134

  Fragments arranged by size: 

                 812     595     398     285     185     134
 
TatI w'GTAC_w

Cuts at:      0     295    2409 
   Size:        295    2114
 
Tth111I GACn'n_nGTC

Cuts at:      0     667    2409 
   Size:        667    1742
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     537    1125    2409 
   Size:        537     588    1284

  Fragments arranged by size: 

                1284     588     537
 
XmaI C'CCGG_G

Cuts at:      0     751    2409 
   Size:        751    1658
 
XmnI GAAnn'nnTTC

Cuts at:      0     284    1426    2077    2409 
   Size:        284    1142     651     332

  Fragments arranged by size: 

                1142     651     332     284
 
ZraI GAC'GTC

Cuts at:      0    2274    2409 
   Size:       2274     135
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AhdI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BclI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI
    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI     DrdI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    Hin4I   HincII  HindIII
     HpaI     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
    PflMI     PfoI    PshAI   PspOMI     PsrI     PstI    PvuII     SalI
     SapI    SexAI     SfcI    SgrAI     SmaI     SspI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII   AflIII     AgeI     AleI     AlfI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI   BssHII    BstBI   BstEII  BstZ17I    CspCI   DraIII
     EagI  EcoICRI    EcoNI     FseI    FspAI    HaeII     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PvuI    RsrII     SacI    SacII    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI     TstI     XbaI     XhoI
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