Restriction map for pF1KSDA0173
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccII       AflII       AflIII      
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       FseI        HaeII       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          861  GTCTAC               GT^[AC][GT]AC
AccIII       3200  TCCGGA               T^CCGGA
AclI         1744  AACGTT               AA^CGTT
Alw44I        782  GTGCAC               G^TGCAC
ApaI          201  GGGCCC               GGGCC^C
ApaLI         782  GTGCAC               G^TGCAC
Asp700I      2061  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          183  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2117  TGCGCA               TGC^GCA
BamHI         984  GGATCC               G^GATCC
BcnI          324  CCCGG                CC^[GC]GG
BglII        3192  AGATCT               A^GATCT
BsaAI        2036  CACGTA               [CT]AC^GT[GA]
BseAI        3200  TCCGGA               T^CCGGA
BsgI         2924  CTGCAC               GTGCAG(16/14)
BspEI        3200  TCCGGA               T^CCGGA
BspHI        1698  TCATGA               T^CATGA
BsrBI         357  CCGCTC               CCGCTC(-3/-3)
BsrDI         285  CATTGC               GCAATG(2/0)
BsrFI        2015  ACCGGC               [GA]^CCGG[CT]
BstEII       1924  GGTCACC              G^GT[ACGT]ACC
BstPI        1924  GGTCACC              G^GT[ACGT]ACC
CfoI         2118  GCGC                 GCG^C
Cfr10I       2015  ACCGGC               [GA]^CCGG[CT]
DrdI         1228  GACAATACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2094  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1924  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2984  ATGCAT               ATGCA^T
FspI         2117  TGCGCA               TGC^GCA
HhaI         2118  GCGC                 GCG^C
HinP1I       2118  GCGC                 G^CGC
HindIII      3178  AAGCTT               A^AGCTT
MroI         3200  TCCGGA               T^CCGGA
NciI          324  CCCGG                CC^[GC]GG
NheI          868  GCTAGC               G^CTAGC
NsiI         2984  ATGCAT               ATGCA^T
PaeR7I       2177  CTCGAG               C^TCGAG
PflFI         183  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2893  GACAAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1744  AACGTT               AA^CGTT
PstI         2853  CTGCAG               CTGCA^G
RcaI         1698  TCATGA               T^CATGA
SpeI         1542  ACTAGT               A^CTAGT
StuI          207  AGGCCT               AGG^CCT
Tth111I       183  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1657  TCTAGA               T^CTAGA
XhoI         2177  CTCGAG               C^TCGAG
XmnI         2061  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaI         1772  CTCGGG               C^[CT]CG[GA]G
             2177  CTCGAG              
BanI          807  GGTGCC               G^G[CT][GA]CC
             1204  GGTGCC              
BbvCI           5  CCTCAGC              CCTCAGC(-5/-2)
             1328  CCTCAGC             
BclI         2970  TGATCA               T^GATCA
             3512  TGATCA              
BsaI         1440  GAGACC               GGTCTC(1/5)
             3260  GGTCTC              
BsaWI         917  ACCGGA               [AT]^CCGG[AT]
             3200  TCCGGA              
BsmI         2448  GAATGC               GAATGC(1/-1)
             1259  GCATTC              
BsoBI        1772  CTCGGG               C^[CT]CG[GA]G
             2177  CTCGAG              
BstXI        1412  CCATCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1454  CCAGGGAGCTGG        
EcoRV        2758  GATATC               GAT^ATC
             2830  GATATC              
FauI         1400  CCCGC                CCCGC(4/6)
             1191  GCGGG               
FbaI         2970  TGATCA               T^GATCA
             3512  TGATCA              
HincII        796  GTTGAC               GT[CT]^[GA]AC
             2719  GTCAAC              
HindII        796  GTTGAC               GT[CT]^[GA]AC
             2719  GTCAAC              
MseI         1618  TTAA                 T^TAA
             3182  TTAA                
MslI          681  CACGCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1279  CATGCCAGTG          
NspI         1278  ACATGC               [GA]CATG^[CT]
             2916  GCATGT              
SapI          582  GAAGAGC              GCTCTTC(1/4)
             2394  GAAGAGC             
Tru9I        1618  TTAA                 T^TAA
             3182  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        Hin1I       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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