Restriction map for pF1KSDA0173
 (Linear) (Six-base) MAPSORT of: KIAA0173.seq  Check: 1667  from: 1  to: 3597



KIAA0173.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AccI GT'mk_AC

Cuts at:      0     862    3597 
   Size:        862    2735
 
AclI AA'CG_TT

Cuts at:      0    1745    3597 
   Size:       1745    1852
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     736    1544    2527    3300    3401    3597 
   Size:        560     176     808     983     773     101     196

  Fragments arranged by size: 

                 983     808     773     560     196     176     101
 
AleI CACnn'nnGTG

Cuts at:      0     685    3597 
   Size:        685    2912
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     703    2939    3597 
   Size:        703    2236     658

  Fragments arranged by size: 

                2236     703     658
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     988    1020    3597 
   Size:        988      32    2577

  Fragments arranged by size: 

                2577     988      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      85     122     305     390     562     714    1495    2852 
   Size:         85      37     183      85     172     152     781    1357

Cuts at:   2852    3597 
   Size:        745

  Fragments arranged by size: 

                1357     781     745     183     172     152      85      85
                  37
 
ApaI G_GGCC'C

Cuts at:      0     205    3597 
   Size:        205    3392
 
ApaLI G'TGCA_C

Cuts at:      0     782    3597 
   Size:        782    2815
 
ApoI r'AATT_y

Cuts at:      0    1673    2963    3074    3597 
   Size:       1673    1290     111     523

  Fragments arranged by size: 

                1673    1290     523     111
 
AvaI C'yCGr_G

Cuts at:      0    1772    2177    3597 
   Size:       1772     405    1420

  Fragments arranged by size: 

                1772    1420     405
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2233    2266    3597 
   Size:       2233      33    1331

  Fragments arranged by size: 

                2233    1331      33
 
BamHI G'GATC_C

Cuts at:      0     984    3597 
   Size:        984    2613
 
BanI G'GyrC_C

Cuts at:      0     807    1204    3597 
   Size:        807     397    2393

  Fragments arranged by size: 

                2393     807     397
 
BanII G_rGCy'C

Cuts at:      0     205     418    1292    2609    3485    3595    3597 
   Size:        205     213     874    1317     876     110       2

  Fragments arranged by size: 

                1317     876     874     213     205     110       2
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     492    1230    1565    3389    3415    3597 
   Size:        492     738     335    1824      26     182

  Fragments arranged by size: 

                1824     738     492     335     182      26
 
BbvCI CC'TCA_GC

Cuts at:      0       6    1329    3597 
   Size:          6    1323    2268

  Fragments arranged by size: 

                2268    1323       6
 
BclI T'GATC_A

Cuts at:      0    2970    3512    3597 
   Size:       2970     542      85

  Fragments arranged by size: 

                2970     542      85
 
BfrBI ATG'CAT

Cuts at:      0    2986    3597 
   Size:       2986     611
 
BglI GCCn_nnn'nGGC

Cuts at:      0     215     481     937    1393    2054    3597 
   Size:        215     266     456     456     661    1543

  Fragments arranged by size: 

                1543     661     456     456     266     215
 
BglII A'GATC_T

Cuts at:      0    3192    3597 
   Size:       3192     405
 
Bme1580I G_kGCm'C

Cuts at:      0     205     786     812     849    2505    3597 
   Size:        205     581      26      37    1656    1092

  Fragments arranged by size: 

                1656    1092     581     205      37      26
 
BmgBI CAC'GTC

Cuts at:      0    3300    3597 
   Size:       3300     297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2076    3440    3597 
   Size:       2076    1364     157

  Fragments arranged by size: 

                2076    1364     157
 
BmtI G_CTAG'C

Cuts at:      0     872    3597 
   Size:        872    2725
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1482    1617    1629    3597 
   Size:       1482     135      12    1968

  Fragments arranged by size: 

                1968    1482     135      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     412     794    1337    1486    2727    2894    3236 
   Size:        345      67     382     543     149    1241     167     342

Cuts at:   3236    3597 
   Size:        361

  Fragments arranged by size: 

                1241     543     382     361     345     342     167     149
                  67
 
Bpu10I CC'TnA_GC

Cuts at:      0       6    1329    2527    3597 
   Size:          6    1323    1198    1070

  Fragments arranged by size: 

                1323    1198    1070       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2611    2683    3398    3597 
   Size:       2611      72     715     199

  Fragments arranged by size: 

                2611     715     199      72
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1434    3266    3597 
   Size:       1434    1832     331

  Fragments arranged by size: 

                1832    1434     331
 
BsaAI yAC'GTr

Cuts at:      0    2038    3597 
   Size:       2038    1559
 
BsaHI Gr'CG_yC

Cuts at:      0     218    2105    2552    3597 
   Size:        218    1887     447    1045

  Fragments arranged by size: 

                1887    1045     447     218
 
BsaWI w'CCGG_w

Cuts at:      0     917    3200    3597 
   Size:        917    2283     397

  Fragments arranged by size: 

                2283     917     397
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202     305     335     343     373     988    1018 
   Size:        172      30     103      30       8      30     615      30

Cuts at:   1018    3597 
   Size:       2579

  Fragments arranged by size: 

                2579     615     172     103      30      30      30      30
                   8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     516    1317    1462    1602    1603    1606    3084    3597 
   Size:        516     801     145     140       1       3    1478     513

  Fragments arranged by size: 

                1478     801     516     513     145     140       3       1
            
 
BseYI C'CCAG_C

Cuts at:      0     227     250     512     562     831    1096    1419    1633 
   Size:        227      23     262      50     269     265     323     214

Cuts at:   1633    1911    1919    2845    3339    3484    3597 
   Size:        278       8     926     494     145     113

  Fragments arranged by size: 

                 926     494     323     278     269     265     262     227
                 214     145     113      50      23       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2909    3597 
   Size:       2909     688
 
BsiHKAI G_wGCw'C

Cuts at:      0     786     960    1612    1837    3523    3597 
   Size:        786     174     652     225    1686      74

  Fragments arranged by size: 

                1686     786     652     225     174      74
 
BsmI GAATG_Cn'

Cuts at:      0    1259    2454    3597 
   Size:       1259    1195    1143

  Fragments arranged by size: 

                1259    1195    1143
 
Bsp1286I G_dGCh'C

Cuts at:      0     205     418     786     812     849     960    1292    1612 
   Size:        205     213     368      26      37     111     332     320

Cuts at:   1612    1837    2505    2609    3485    3523    3595    3597 
   Size:        225     668     104     876      38      72       2

  Fragments arranged by size: 

                 876     668     368     332     320     225     213     205
                 111     104      72      38      37      26       2
 
BspEI T'CCGG_A

Cuts at:      0    3200    3597 
   Size:       3200     397
 
BspHI T'CATG_A

Cuts at:      0    1698    3597 
   Size:       1698    1899
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      93    2300    2794    3597 
   Size:         93    2207     494     803

  Fragments arranged by size: 

                2207     803     494      93
 
BsrBI CCG'CTC

Cuts at:      0     359    3597 
   Size:        359    3238
 
BsrDI GCAATG_nn'

Cuts at:      0     284    3597 
   Size:        284    3313
 
BsrFI r'CCGG_y

Cuts at:      0    2015    3597 
   Size:       2015    1582
 
BstEII G'GTnAC_C

Cuts at:      0    1924    3597 
   Size:       1924    1673
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1419    1461    3597 
   Size:       1419      42    2136

  Fragments arranged by size: 

                2136    1419      42
 
BstYI r'GATC_y

Cuts at:      0     611     737     984    2297    3192    3597 
   Size:        611     126     247    1313     895     405

  Fragments arranged by size: 

                1313     895     611     405     247     126
 
Bsu36I CC'TnA_GG

Cuts at:      0      72     128    1166    1445    3597 
   Size:         72      56    1038     279    2152

  Fragments arranged by size: 

                2152    1038     279      72      56
 
BtgI C'CryG_G

Cuts at:      0     275     494     506     518    3057    3168    3597 
   Size:        275     219      12      12    2539     111     429

  Fragments arranged by size: 

                2539     429     275     219     111      12      12
 
BtsI GCAGTG_nn'

Cuts at:      0     470    2921    3597 
   Size:        470    2451     676

  Fragments arranged by size: 

                2451     676     470
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      45      80     879     914    3597 
   Size:         45      35     799      35    2683

  Fragments arranged by size: 

                2683     799      45      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1234    3597 
   Size:       1234    2363
 
EaeI y'GGCC_r

Cuts at:      0     670    1394    1646    3597 
   Size:        670     724     252    1951

  Fragments arranged by size: 

                1951     724     670     252
 
EarI CTCTTCn'nnn_

Cuts at:      0     577     633     909    1413    1580    2389    3597 
   Size:        577      56     276     504     167     809    1208

  Fragments arranged by size: 

                1208     809     577     504     276     167      56
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28    2040    3597 
   Size:         28    2012    1557

  Fragments arranged by size: 

                2012    1557      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     412     560     736     794    1337    1486    1544 
   Size:        345      67     148     176      58     543     149      58

Cuts at:   1544    2527    2727    2894    3236    3300    3401    3597 
   Size:        983     200     167     342      64     101     196

  Fragments arranged by size: 

                 983     543     345     342     200     196     176     167
                 149     148     101      67      64      58      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2098    3597 
   Size:       2098    1499
 
EcoO109I rG'GnC_Cy

Cuts at:      0     711    1194    1357    2231    2709    3597 
   Size:        711     483     163     874     478     888

  Fragments arranged by size: 

                 888     874     711     483     478     163
 
EcoRV GAT'ATC

Cuts at:      0    2760    2832    3597 
   Size:       2760      72     765

  Fragments arranged by size: 

                2760     765      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3439    3597 
   Size:       3439     158
 
FspI TGC'GCA

Cuts at:      0    2119    3597 
   Size:       2119    1478
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     603     635     955     987    1165    1197 
   Size:        172      32     399      32     320      32     178      32

Cuts at:   1197    1706    1738    3597 
   Size:        509      32    1859

  Fragments arranged by size: 

                1859     509     399     320     178     172      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     798    2721    3597 
   Size:        798    1923     876

  Fragments arranged by size: 

                1923     876     798
 
HindIII A'AGCT_T

Cuts at:      0    3178    3597 
   Size:       3178     419
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1012    1205    1737    3597 
   Size:       1012     193     532    1860

  Fragments arranged by size: 

                1860    1012     532     193
 
MscI TGG'CCA

Cuts at:      0     672    1396    1648    3597 
   Size:        672     724     252    1949

  Fragments arranged by size: 

                1949     724     672     252
 
MslI CAynn'nnrTG

Cuts at:      0     685    1283    3597 
   Size:        685     598    2314

  Fragments arranged by size: 

                2314     685     598
 
MspA1I CmG'CkG

Cuts at:      0     559     835    1419    2276    2852    3597 
   Size:        559     276     584     857     576     745

  Fragments arranged by size: 

                 857     745     584     576     559     276
 
NcoI C'CATG_G

Cuts at:      0     494     506     518    3168    3597 
   Size:        494      12      12    2650     429

  Fragments arranged by size: 

                2650     494     429      12      12
 
NheI G'CTAG_C

Cuts at:      0     868    3597 
   Size:        868    2729
 
NsiI A_TGCA'T

Cuts at:      0    2988    3597 
   Size:       2988     609
 
NspI r_CATG'y

Cuts at:      0    1282    2920    3597 
   Size:       1282    1638     677

  Fragments arranged by size: 

                1638    1282     677
 
PasI CC'CwG_GG

Cuts at:      0    1799    2705    2887    3597 
   Size:       1799     906     182     710

  Fragments arranged by size: 

                1799     906     710     182
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     390     519    3152    3597 
   Size:        390     129    2633     445

  Fragments arranged by size: 

                2633     445     390     129
 
PfoI T'CCnGG_A

Cuts at:      0     323    2711    3597 
   Size:        323    2388     886

  Fragments arranged by size: 

                2388     886     323
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     530     562     988    1020    3597 
   Size:        530      32     426      32    2577

  Fragments arranged by size: 

                2577     530     426      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1194    1357    2709    3597 
   Size:       1194     163    1352     888

  Fragments arranged by size: 

                1352    1194     888     163
 
PshAI GACnn'nnGTC

Cuts at:      0    2897    3597 
   Size:       2897     700
 
PspOMI G'GGCC_C

Cuts at:      0     201    3597 
   Size:        201    3396
 
PspXI vC'TCGA_Gb

Cuts at:      0    2177    3597 
   Size:       2177    1420
 
PstI C_TGCA'G

Cuts at:      0    2857    3597 
   Size:       2857     740
 
PvuII CAG'CTG

Cuts at:      0     835    1419    2852    3597 
   Size:        835     584    1433     745

  Fragments arranged by size: 

                1433     835     745     584
 
SapI GCTCTTCn'nnn_

Cuts at:      0     577    2389    3597 
   Size:        577    1812    1208

  Fragments arranged by size: 

                1812    1208     577
 
SfcI C'TryA_G

Cuts at:      0      24     366    1728    2430    2649    2853    3597 
   Size:         24     342    1362     702     219     204     744

  Fragments arranged by size: 

                1362     744     702     342     219     204      24
 
SmlI C'TyrA_G

Cuts at:      0    2177    2626    2698    3413    3597 
   Size:       2177     449      72     715     184

  Fragments arranged by size: 

                2177     715     449     184      72
 
SpeI A'CTAG_T

Cuts at:      0    1542    3597 
   Size:       1542    2055
 
StuI AGG'CCT

Cuts at:      0     209    3597 
   Size:        209    3388
 
StyI C'CwwG_G

Cuts at:      0     494     506     518    1217    2400    3168    3597 
   Size:        494      12      12     699    1183     768     429

  Fragments arranged by size: 

                1183     768     699     494     429      12      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     747    1173    3163    3597 
   Size:        747     426    1990     434

  Fragments arranged by size: 

                1990     747     434     426
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     286     318     722     754     868     900    3597 
   Size:        286      32     404      32     114      32    2697

  Fragments arranged by size: 

                2697     404     286     114      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     186    3597 
   Size:        186    3411
 
XbaI T'CTAG_A

Cuts at:      0    1657    3597 
   Size:       1657    1940
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     492    1641    2665    2751    3597 
   Size:        492    1149    1024      86     846

  Fragments arranged by size: 

                1149    1024     846     492      86
 
XhoI C'TCGA_G

Cuts at:      0    2177    3597 
   Size:       2177    1420
 
XmnI GAAnn'nnTTC

Cuts at:      0    2065    3597 
   Size:       2065    1532
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NheI
     NsiI     NspI     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII     SapI     SfcI     SmlI     SpeI
     StuI     StyI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AscI     AseI    AsiSI    AvrII     BbeI     BcgI    BciVI     BlpI
    BsaBI    BsiEI    BsiWI    BsmBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII     EagI  EcoICRI
    EcoRI     FseI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI
     PciI     PmeI     PmlI     PsiI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
     TatI     XmaI     ZraI
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