Restriction map for
pF1KSDA0168
(Linear) (Six-base) MAPSORT of: KIAA0168.seq Check: 5448 from: 1 to: 978
KIAA0168.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 316 409 634 724 978
Size: 100 216 93 225 90 254
Fragments arranged by size:
254 225 216 100 93 90
AlwNI CAG_nnn'CTG
Cuts at: 0 612 702 945 978
Size: 612 90 243 33
Fragments arranged by size:
612 243 90 33
ApaI G_GGCC'C
Cuts at: 0 748 978
Size: 748 230
ApoI r'AATT_y
Cuts at: 0 111 631 645 978
Size: 111 520 14 333
Fragments arranged by size:
520 333 111 14
BanI G'GyrC_C
Cuts at: 0 125 978
Size: 125 853
BanII G_rGCy'C
Cuts at: 0 164 282 748 978
Size: 164 118 466 230
Fragments arranged by size:
466 230 164 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 146 978
Size: 88 58 832
Fragments arranged by size:
832 88 58
BglII A'GATC_T
Cuts at: 0 323 758 978
Size: 323 435 220
Fragments arranged by size:
435 323 220
Bme1580I G_kGCm'C
Cuts at: 0 314 748 978
Size: 314 434 230
Fragments arranged by size:
434 314 230
BmgBI CAC'GTC
Cuts at: 0 813 978
Size: 813 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 978
Size: 813 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 777 978
Size: 777 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 724 853 967 978
Size: 363 361 129 114 11
Fragments arranged by size:
363 361 129 114 11
Bpu10I CC'TnA_GC
Cuts at: 0 281 978
Size: 281 697
BsaI GGTCTCn'nnnn_
Cuts at: 0 955 978
Size: 955 23
BsaAI yAC'GTr
Cuts at: 0 672 978
Size: 672 306
BsaHI Gr'CG_yC
Cuts at: 0 469 495 978
Size: 469 26 483
Fragments arranged by size:
483 469 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 978
Size: 490 30 458
Fragments arranged by size:
490 458 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 240 670 964 978
Size: 237 3 430 294 14
Fragments arranged by size:
430 294 237 14 3
BseYI C'CCAG_C
Cuts at: 0 966 978
Size: 966 12
BsiHKAI G_wGCw'C
Cuts at: 0 923 978
Size: 923 55
BsmI GAATG_Cn'
Cuts at: 0 227 978
Size: 227 751
Bsp1286I G_dGCh'C
Cuts at: 0 164 282 314 748 923 978
Size: 164 118 32 434 175 55
Fragments arranged by size:
434 175 164 118 55 32
BsrDI GCAATG_nn'
Cuts at: 0 465 978
Size: 465 513
BsrFI r'CCGG_y
Cuts at: 0 188 840 978
Size: 188 652 138
Fragments arranged by size:
652 188 138
BstEII G'GTnAC_C
Cuts at: 0 352 978
Size: 352 626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 978
Size: 685 293
BstYI r'GATC_y
Cuts at: 0 323 758 978
Size: 323 435 220
Fragments arranged by size:
435 323 220
BtgI C'CryG_G
Cuts at: 0 453 978
Size: 453 525
BtsI GCAGTG_nn'
Cuts at: 0 438 978
Size: 438 540
DraIII CAC_nnn'GTG
Cuts at: 0 433 978
Size: 433 545
EaeI y'GGCC_r
Cuts at: 0 516 978
Size: 516 462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 316 363 409 634 724 853 967
Size: 100 216 47 46 225 90 129 114
Cuts at: 967 978
Size: 11
Fragments arranged by size:
225 216 129 114 100 90 47 46
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 978
Size: 400 578
EcoO109I rG'GnC_Cy
Cuts at: 0 180 384 609 744 978
Size: 180 204 225 135 234
Fragments arranged by size:
234 225 204 180 135
EcoRI G'AATT_C
Cuts at: 0 645 978
Size: 645 333
FspI TGC'GCA
Cuts at: 0 424 907 978
Size: 424 483 71
Fragments arranged by size:
483 424 71
FspAI rTGC'GCAy
Cuts at: 0 424 978
Size: 424 554
HindIII A'AGCT_T
Cuts at: 0 852 978
Size: 852 126
MslI CAynn'nnrTG
Cuts at: 0 683 978
Size: 683 295
MspA1I CmG'CkG
Cuts at: 0 417 483 723 978
Size: 417 66 240 255
Fragments arranged by size:
417 255 240 66
NaeI GCC'GGC
Cuts at: 0 190 978
Size: 190 788
NgoMIV G'CCGG_C
Cuts at: 0 188 978
Size: 188 790
PflMI CCAn_nnn'nTGG
Cuts at: 0 559 978
Size: 559 419
PfoI T'CCnGG_A
Cuts at: 0 869 978
Size: 869 109
PpuMI rG'GwC_Cy
Cuts at: 0 609 978
Size: 609 369
PspOMI G'GGCC_C
Cuts at: 0 744 978
Size: 744 234
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 814 846 978
Size: 814 32 132
Fragments arranged by size:
814 132 32
PstI C_TGCA'G
Cuts at: 0 206 401 978
Size: 206 195 577
Fragments arranged by size:
577 206 195
PvuII CAG'CTG
Cuts at: 0 417 978
Size: 417 561
SbfI CC_TGCA'GG
Cuts at: 0 401 978
Size: 401 577
SexAI A'CCwGG_T
Cuts at: 0 788 978
Size: 788 190
SfcI C'TryA_G
Cuts at: 0 6 202 397 978
Size: 6 196 195 581
Fragments arranged by size:
581 196 195 6
TatI w'GTAC_w
Cuts at: 0 95 911 978
Size: 95 816 67
Fragments arranged by size:
816 95 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 459 490 491 522 978
Size: 4 32 423 31 1 31 456
Fragments arranged by size:
456 423 32 31 31 4 1
XmnI GAAnn'nnTTC
Cuts at: 0 227 978
Size: 227 751
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanI BanII BbsI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BstEII BstXI BstYI BtgI BtsI DraIII EaeI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HindIII MslI
MspA1I NaeI NgoMIV PflMI PfoI PpuMI PspOMI PsrI
PstI PvuII SbfI SexAI SfcI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmtI BpuEI BsaBI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoRV FalI FseI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI