Restriction map for pF1KSDA0157
 (Linear) (Six-base) MAPSORT of: KIAA0157.seq  Check: 7226  from: 1  to: 1245



KIAA0157.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AclI AA'CG_TT

Cuts at:      0     109    1245 
   Size:        109    1136
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     372     426     512     631     890    1066    1245 
   Size:         12     360      54      86     119     259     176     179

  Fragments arranged by size: 

                 360     259     179     176     119      86      54      12
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     823    1245 
   Size:        823     422
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     817     849    1245 
   Size:        817      32     396

  Fragments arranged by size: 

                 817     396      32
 
ApoI r'AATT_y

Cuts at:      0     145    1134    1245 
   Size:        145     989     111

  Fragments arranged by size: 

                 989     145     111
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1033    1066    1245 
   Size:       1033      33     179

  Fragments arranged by size: 

                1033     179      33
 
BamHI G'GATC_C

Cuts at:      0     906    1245 
   Size:        906     339
 
BanII G_rGCy'C

Cuts at:      0    1015    1245 
   Size:       1015     230
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     518     741    1245 
   Size:        518     223     504

  Fragments arranged by size: 

                 518     504     223
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     884     918    1245 
   Size:        884      34     327

  Fragments arranged by size: 

                 884     327      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1061    1245 
   Size:       1061     184
 
Bme1580I G_kGCm'C

Cuts at:      0     368    1245 
   Size:        368     877
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      94    1245 
   Size:         94    1151
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     983    1245 
   Size:        983     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    1245 
   Size:       1115     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1245 
   Size:        986     259
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1245 
   Size:          7    1238
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     902    1067    1245 
   Size:        244     658     165     178

  Fragments arranged by size: 

                 658     244     178     165
 
BseYI C'CCAG_C

Cuts at:      0     828    1245 
   Size:        828     417
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    1245 
   Size:        692     553
 
BsiHKAI G_wGCw'C

Cuts at:      0     440    1245 
   Size:        440     805
 
Bsp1286I G_dGCh'C

Cuts at:      0     368     440    1015    1245 
   Size:        368      72     575     230

  Fragments arranged by size: 

                 575     368     230      72
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     318    1245 
   Size:        318     927
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     670    1245 
   Size:        670     575
 
BstYI r'GATC_y

Cuts at:      0     906    1245 
   Size:        906     339
 
BtgI C'CryG_G

Cuts at:      0    1196    1245 
   Size:       1196      49
 
BtsI GCAGTG_nn'

Cuts at:      0     401     671     797    1245 
   Size:        401     270     126     448

  Fragments arranged by size: 

                 448     401     270     126
 
EarI CTCTTCn'nnn_

Cuts at:      0     391     445    1020    1245 
   Size:        391      54     575     225

  Fragments arranged by size: 

                 575     391     225      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12     372     426     512     631     890    1066    1115 
   Size:         12     360      54      86     119     259     176      49

Cuts at:   1115    1245 
   Size:        130

  Fragments arranged by size: 

                 360     259     176     130     119      86      54      49
                  12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1215    1245 
   Size:       1215      30
 
EcoRV GAT'ATC

Cuts at:      0     473    1245 
   Size:        473     772
 
HaeII r_GCGC'y

Cuts at:      0      53    1245 
   Size:         53    1192
 
HindIII A'AGCT_T

Cuts at:      0     819    1245 
   Size:        819     426
 
MspA1I CmG'CkG

Cuts at:      0     868    1093    1245 
   Size:        868     225     152

  Fragments arranged by size: 

                 868     225     152
 
NcoI C'CATG_G

Cuts at:      0    1196    1245 
   Size:       1196      49
 
PfoI T'CCnGG_A

Cuts at:      0    1053    1245 
   Size:       1053     192
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346    1245 
   Size:        314      32     899

  Fragments arranged by size: 

                 899     314      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1215    1245 
   Size:       1215      30
 
PsiI TTA'TAA

Cuts at:      0     638    1245 
   Size:        638     607
 
PvuII CAG'CTG

Cuts at:      0     868    1093    1245 
   Size:        868     225     152

  Fragments arranged by size: 

                 868     225     152
 
SapI GCTCTTCn'nnn_

Cuts at:      0     445    1020    1245 
   Size:        445     575     225

  Fragments arranged by size: 

                 575     445     225
 
ScaI AGT'ACT

Cuts at:      0     954    1245 
   Size:        954     291
 
SfcI C'TryA_G

Cuts at:      0     318    1148    1245 
   Size:        318     830      97

  Fragments arranged by size: 

                 830     318      97
 
SmlI C'TyrA_G

Cuts at:      0    1001    1245 
   Size:       1001     244
 
StuI AGG'CCT

Cuts at:      0     901    1245 
   Size:        901     344
 
StyI C'CwwG_G

Cuts at:      0    1196    1245 
   Size:       1196      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     266     950    1245 
   Size:        266     684     295

  Fragments arranged by size: 

                 684     295     266
 
TatI w'GTAC_w

Cuts at:      0     515     880     952    1245 
   Size:        515     365      72     293

  Fragments arranged by size: 

                 515     365     293      72
 
Tth111I GACn'n_nGTC

Cuts at:      0     373    1245 
   Size:        373     872
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1090    1245 
   Size:       1090     155
 

 Enzymes that do cut:

     AclI     AcuI     AlfI     AloI     ApoI     BaeI    BamHI    BanII
     BbsI     BcgI    BciVI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstYI     BtgI     BtsI     EarI  Eco57MI EcoO109I    EcoRV    HaeII
  HindIII   MspA1I     NcoI     PfoI     PpiI    PpuMI     PsiI    PvuII
     SapI     ScaI     SfcI     SmlI     StuI     StyI    TaqII     TatI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BanI     BbeI    BbvCI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI
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