Restriction map for pF1KSDA0153
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AflII       AflIII      AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstBI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HinfI       HpaI        KasI        
KspI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshBI       PvuI        
RsrII       SacI        SacII       SapI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SspBI       SspI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       1850  GGTACC               G^GTACC
AccIII        752  TCCGGA               T^CCGGA
AclI          137  AACGTT               AA^CGTT
AcsI         1621  GAATTT               [GA]^AATT[CT]
AocI          684  CCTGAGG              CC^T[ACGT]AGG
ApoI         1621  GAATTT               [GA]^AATT[CT]
Asp718I      1850  GGTACC               G^GTACC
AviII        1178  TGCGCA               TGC^GCA
BamHI         599  GGATCC               G^GATCC
BglI         1139  GCCCCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1649  AGATCT               A^GATCT
BsaWI         752  TCCGGA               [AT]^CCGG[AT]
BseAI         752  TCCGGA               T^CCGGA
BsmBI         736  GAGACG               CGTCTC(1/5)
BspEI         752  TCCGGA               T^CCGGA
BspHI        1541  TCATGA               T^CATGA
BssSI         172  CACGAG               CACGAG(-5/-1)
BstAPI        405  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        711  GGTGACC              G^GT[ACGT]ACC
BstPI         711  GGTGACC              G^GT[ACGT]ACC
Bsu36I        684  CCTGAGG              CC^T[ACGT]AGG
DrdI         1282  GACACCCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        684  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       711  GGTGACC              G^GT[ACGT]ACC
FspI         1178  TGCGCA               TGC^GCA
HincII       1747  GTCGAC               GT[CT]^[GA]AC
HindII       1747  GTCGAC               GT[CT]^[GA]AC
KpnI         1850  GGTACC               GGTAC^C
MroI          752  TCCGGA               T^CCGGA
NaeI           26  GCCGGC               GCC^GGC
NcoI          540  CCATGG               C^CATGG
NgoMIV         26  GCCGGC               G^CCGGC
PshAI        1632  GACGGACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      137  AACGTT               AA^CGTT
RcaI         1541  TCATGA               T^CATGA
SalI         1747  GTCGAC               G^TCGAC
SbfI         1844  CCTGCAGG             CCTGCA^GG
SfiI         1138  GGCCCCGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          761  GCATGC               GCATG^C
Sse8387I     1844  CCTGCAGG             CCTGCA^GG
StuI           77  AGGCCT               AGG^CCT
Tsp509I      1622  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII        1636  GACGTC               GACGT^C
             1873  GACGTC              
AccI          919  GTCTAC               GT^[AC][GT]AC
             1747  GTCGAC              
Alw44I        892  GTGCAC               G^TGCAC
             1576  GTGCAC              
ApaI          776  GGGCCC               GGGCC^C
             1146  GGGCCC              
ApaLI         892  GTGCAC               G^TGCAC
             1576  GTGCAC              
Asp700I      1068  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1527  GAAGCACTTC          
BalI          434  TGGCCA               TGG^CCA
              941  TGGCCA              
BbsI         1384  GAAGAC               GAAGAC(2/6)
             1876  GTCTTC              
BbvCI         798  CCTCAGC              CCTCAGC(-5/-2)
              550  GCTGAGG             
BpuAI        1384  GAAGAC               GAAGAC(2/6)
             1876  GTCTTC              
BsmAI         719  GAGAC                GTCTC(1/5)
              736  GAGAC               
BsrBI          10  GAGCGG               CCGCTC(-3/-3)
              318  GAGCGG              
BsrFI          26  GCCGGC               [GA]^CCGG[CT]
              564  GCCGGT              
BstXI         437  CCAACCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1899  CCAGCCCGGTGG        
Cfr10I         26  GCCGGC               [GA]^CCGG[CT]
              564  GCCGGT              
MluNI         434  TGGCCA               TGG^CCA
              941  TGGCCA              
MscI          434  TGGCCA               TGG^CCA
              941  TGGCCA              
PstI          403  CTGCAG               CTGCA^G
             1845  CTGCAG              
XmnI         1068  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1527  GAAGCACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI        
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