Restriction map for pF1KSDA0153
 (Linear) (Six-base) MAPSORT of: KIAA0153.seq  Check: 564  from: 1  to: 1932



KIAA0153.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     399     648     927    1123    1563    1932 
   Size:        399     249     279     196     440     369

  Fragments arranged by size: 

                 440     399     369     279     249     196
 
AatII G_ACGT'C

Cuts at:      0    1640    1877    1932 
   Size:       1640     237      55

  Fragments arranged by size: 

                1640     237      55
 
AccI GT'mk_AC

Cuts at:      0     920    1748    1932 
   Size:        920     828     184

  Fragments arranged by size: 

                 920     828     184
 
Acc65I G'GTAC_C

Cuts at:      0    1850    1932 
   Size:       1850      82
 
AclI AA'CG_TT

Cuts at:      0     138    1932 
   Size:        138    1794
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1588    1780    1863    1932 
   Size:       1588     192      83      69

  Fragments arranged by size: 

                1588     192      83      69
 
AleI CACnn'nnGTG

Cuts at:      0     928    1932 
   Size:        928    1004
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     784    1204    1932 
   Size:        784     420     728

  Fragments arranged by size: 

                 784     728     420
 
AlwNI CAG_nnn'CTG

Cuts at:      0     402     484    1809    1932 
   Size:        402      82    1325     123

  Fragments arranged by size: 

                1325     402     123      82
 
ApaI G_GGCC'C

Cuts at:      0     780    1150    1932 
   Size:        780     370     782

  Fragments arranged by size: 

                 782     780     370
 
ApaLI G'TGCA_C

Cuts at:      0     892    1576    1932 
   Size:        892     684     356

  Fragments arranged by size: 

                 892     684     356
 
ApoI r'AATT_y

Cuts at:      0    1621    1932 
   Size:       1621     311
 
AvaI C'yCGr_G

Cuts at:      0      19      48     248     276     415     716     814    1141 
   Size:         19      29     200      28     139     301      98     327

Cuts at:   1141    1597    1730    1932 
   Size:        456     133     202

  Fragments arranged by size: 

                 456     327     301     202     200     139     133      98
                  29      28      19
 
BamHI G'GATC_C

Cuts at:      0     599    1932 
   Size:        599    1333
 
BanI G'GyrC_C

Cuts at:      0     411     633    1319    1850    1932 
   Size:        411     222     686     531      82

  Fragments arranged by size: 

                 686     531     411     222      82
 
BanII G_rGCy'C

Cuts at:      0     326     780    1150    1932 
   Size:        326     454     370     782

  Fragments arranged by size: 

                 782     454     370     326
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1391    1869    1932 
   Size:       1391     478      63

  Fragments arranged by size: 

                1391     478      63
 
BbvCI CC'TCA_GC

Cuts at:      0     551     799    1932 
   Size:        551     248    1133

  Fragments arranged by size: 

                1133     551     248
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1145    1932 
   Size:       1145     787
 
BglII A'GATC_T

Cuts at:      0    1649    1932 
   Size:       1649     283
 
Bme1580I G_kGCm'C

Cuts at:      0     416     620     780     896    1150    1580    1932 
   Size:        416     204     160     116     254     430     352

  Fragments arranged by size: 

                 430     416     352     254     204     160     116
 
BmgBI CAC'GTC

Cuts at:      0     374     615     930    1290    1389    1932 
   Size:        374     241     315     360      99     543

  Fragments arranged by size: 

                 543     374     360     315     241      99
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     153     467    1188    1932 
   Size:        153     314     721     744

  Fragments arranged by size: 

                 744     721     314     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     310     520     868    1831    1932 
   Size:        187     123     210     348     963     101

  Fragments arranged by size: 

                 963     348     210     187     123     101
 
Bpu10I CC'TnA_GC

Cuts at:      0     551     799    1932 
   Size:        551     248    1133

  Fragments arranged by size: 

                1133     551     248
 
BsaHI Gr'CG_yC

Cuts at:      0      58     992    1637    1874    1932 
   Size:         58     934     645     237      58

  Fragments arranged by size: 

                 934     645     237      58      58
 
BsaWI w'CCGG_w

Cuts at:      0     752    1932 
   Size:        752    1180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     493     523    1932 
   Size:        493      30    1409

  Fragments arranged by size: 

                1409     493      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     232     244     247     517     568     721    1932 
   Size:         79     153      12       3     270      51     153    1211

  Fragments arranged by size: 

                1211     270     153     153      79      51      12       3
            
 
BseYI C'CCAG_C

Cuts at:      0     187     619    1932 
   Size:        187     432    1313

  Fragments arranged by size: 

                1313     432     187
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     142     409     952    1288    1932 
   Size:         85      57     267     543     336     644

  Fragments arranged by size: 

                 644     543     336     267      85      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     389     833     896    1333    1580    1932 
   Size:        173     216     444      63     437     247     352

  Fragments arranged by size: 

                 444     437     352     247     216     173      63
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     730    1932 
   Size:        730    1202
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     326     389     416     620     780     833     896 
   Size:        173     153      63      27     204     160      53      63

Cuts at:    896    1150    1333    1580    1932 
   Size:        254     183     247     352

  Fragments arranged by size: 

                 352     254     247     204     183     173     160     153
                  63      63      53      27
 
BspEI T'CCGG_A

Cuts at:      0     752    1932 
   Size:        752    1180
 
BspHI T'CATG_A

Cuts at:      0    1541    1932 
   Size:       1541     391
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     399     648     927    1097    1123    1184    1563    1838 
   Size:        399     249     279     170      26      61     379     275

Cuts at:   1838    1930    1932 
   Size:         92       2

  Fragments arranged by size: 

                 399     379     279     275     249     170      92      61
                  26       2
 
BsrBI CCG'CTC

Cuts at:      0      12     320    1932 
   Size:         12     308    1612

  Fragments arranged by size: 

                1612     308      12
 
BsrFI r'CCGG_y

Cuts at:      0      26     564    1932 
   Size:         26     538    1368

  Fragments arranged by size: 

                1368     538      26
 
BssHII G'CGCG_C

Cuts at:      0      71     117     391    1123    1270    1932 
   Size:         71      46     274     732     147     662

  Fragments arranged by size: 

                 732     662     274     147      71      46
 
BssSI C'ACGA_G

Cuts at:      0     172    1932 
   Size:        172    1760
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     411    1932 
   Size:        411    1521
 
BstEII G'GTnAC_C

Cuts at:      0     711    1932 
   Size:        711    1221
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     444    1906    1932 
   Size:        444    1462      26

  Fragments arranged by size: 

                1462     444      26
 
BstYI r'GATC_y

Cuts at:      0     599    1247    1649    1805    1932 
   Size:        599     648     402     156     127

  Fragments arranged by size: 

                 648     599     402     156     127
 
Bsu36I CC'TnA_GG

Cuts at:      0     685    1932 
   Size:        685    1247
 
BtgI C'CryG_G

Cuts at:      0     459     540     629     887     900    1932 
   Size:        459      81      89     258      13    1032

  Fragments arranged by size: 

                1032     459     258      89      81      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1104    1932 
   Size:       1104     828
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1288    1932 
   Size:       1288     644
 
EaeI y'GGCC_r

Cuts at:      0      83     434     903     941    1932 
   Size:         83     351     469      38     991

  Fragments arranged by size: 

                 991     469     351      83      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     221    1009    1580    1932 
   Size:        221     788     571     352

  Fragments arranged by size: 

                 788     571     352     221
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1003    1932 
   Size:       1003     929
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     310     520     868    1588    1780    1831    1863 
   Size:        187     123     210     348     720     192      51      32

Cuts at:   1863    1932 
   Size:         69

  Fragments arranged by size: 

                 720     348     210     192     187     123      69      51
                  32
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     129     690     777    1146    1442    1932 
   Size:         16     113     561      87     369     296     490

  Fragments arranged by size: 

                 561     490     369     296     113      87      16
 
FspI TGC'GCA

Cuts at:      0    1180    1932 
   Size:       1180     752
 
HaeII r_GCGC'y

Cuts at:      0      27     100     115    1932 
   Size:         27      73      15    1817

  Fragments arranged by size: 

                1817      73      27      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     787     819    1708    1740    1932 
   Size:        787      32     889      32     192

  Fragments arranged by size: 

                 889     787     192      32      32
 
HincII GTy'rAC

Cuts at:      0    1749    1932 
   Size:       1749     183
 
KpnI G_GTAC'C

Cuts at:      0    1854    1932 
   Size:       1854      78
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1511    1932 
   Size:       1511     421
 
MscI TGG'CCA

Cuts at:      0     436     943    1932 
   Size:        436     507     989

  Fragments arranged by size: 

                 989     507     436
 
MslI CAynn'nnrTG

Cuts at:      0     211     787     928    1540    1932 
   Size:        211     576     141     612     392

  Fragments arranged by size: 

                 612     576     392     211     141
 
MspA1I CmG'CkG

Cuts at:      0     402     534     550    1218    1932 
   Size:        402     132      16     668     714

  Fragments arranged by size: 

                 714     668     402     132      16
 
NaeI GCC'GGC

Cuts at:      0      28    1932 
   Size:         28    1904
 
NcoI C'CATG_G

Cuts at:      0     540    1932 
   Size:        540    1392
 
NgoMIV G'CCGG_C

Cuts at:      0      26    1932 
   Size:         26    1906
 
NspI r_CATG'y

Cuts at:      0     651     765     792    1932 
   Size:        651     114      27    1140

  Fragments arranged by size: 

                1140     651     114      27
 
PasI CC'CwG_GG

Cuts at:      0     773    1704    1932 
   Size:        773     931     228

  Fragments arranged by size: 

                 931     773     228
 
PfoI T'CCnGG_A

Cuts at:      0    1808    1932 
   Size:       1808     124
 
PpuMI rG'GwC_Cy

Cuts at:      0      16     129     690    1932 
   Size:         16     113     561    1242

  Fragments arranged by size: 

                1242     561     113      16
 
PshAI GACnn'nnGTC

Cuts at:      0    1636    1932 
   Size:       1636     296
 
PspOMI G'GGCC_C

Cuts at:      0     776    1146    1932 
   Size:        776     370     786

  Fragments arranged by size: 

                 786     776     370
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1453    1485    1932 
   Size:       1453      32     447

  Fragments arranged by size: 

                1453     447      32
 
PstI C_TGCA'G

Cuts at:      0     407    1849    1932 
   Size:        407    1442      83

  Fragments arranged by size: 

                1442     407      83
 
PvuII CAG'CTG

Cuts at:      0     402     534     550    1932 
   Size:        402     132      16    1382

  Fragments arranged by size: 

                1382     402     132      16
 
SalI G'TCGA_C

Cuts at:      0    1747    1932 
   Size:       1747     185
 
SanDI GG'GwC_CC

Cuts at:      0      16     129    1932 
   Size:         16     113    1803

  Fragments arranged by size: 

                1803     113      16
 
SbfI CC_TGCA'GG

Cuts at:      0    1849    1932 
   Size:       1849      83
 
SfcI C'TryA_G

Cuts at:      0     403    1029    1845    1932 
   Size:        403     626     816      87

  Fragments arranged by size: 

                 816     626     403      87
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1145    1932 
   Size:       1145     787
 
SmaI CCC'GGG

Cuts at:      0      50     250     278     417    1732    1932 
   Size:         50     200      28     139    1315     200

  Fragments arranged by size: 

                1315     200     200     139      50      28
 
SphI G_CATG'C

Cuts at:      0     765    1932 
   Size:        765    1167
 
SrfI GCCC'GGGC

Cuts at:      0      50     417    1932 
   Size:         50     367    1515

  Fragments arranged by size: 

                1515     367      50
 
StuI AGG'CCT

Cuts at:      0      79    1932 
   Size:         79    1853
 
StyI C'CwwG_G

Cuts at:      0      80     540    1337    1932 
   Size:         80     460     797     595

  Fragments arranged by size: 

                 797     595     460      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     970     991    1300    1932 
   Size:        970      21     309     632

  Fragments arranged by size: 

                 970     632     309      21
 
TatI w'GTAC_w

Cuts at:      0     475    1352    1932 
   Size:        475     877     580

  Fragments arranged by size: 

                 877     580     475
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     363     395     493     525    1775    1807    1932 
   Size:        363      32      98      32    1250      32     125

  Fragments arranged by size: 

                1250     363     125      98      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     538     789    1625    1932 
   Size:        538     251     836     307

  Fragments arranged by size: 

                 836     538     307     251
 
XmaI C'CCGG_G

Cuts at:      0      48     248     276     415    1730    1932 
   Size:         48     200      28     139    1315     202

  Fragments arranged by size: 

                1315     202     200     139      48      28
 
XmnI GAAnn'nnTTC

Cuts at:      0    1072    1531    1932 
   Size:       1072     459     401

  Fragments arranged by size: 

                1072     459     401
 
ZraI GAC'GTC

Cuts at:      0    1638    1875    1932 
   Size:       1638     237      57

  Fragments arranged by size: 

                1638     237      57
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI     PasI     PfoI    PpuMI    PshAI   PspOMI
     PsrI     PstI    PvuII     SalI    SanDI     SbfI     SfcI     SfiI
     SmaI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII   AflIII     AgeI     AhdI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI
     BlpI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BsrDI    BsrGI    BstBI  BstZ17I     BtsI     ClaI
    CspCI     DraI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KasI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI     PvuI    RsrII
     SacI    SacII     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SspI     SwaI  Tth111I     XbaI     XhoI
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