Restriction map for pF1KSDA0146
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccI        AclI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AviII       AvrII       BamHI       BciVI       
BclI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        MamI        MfeI        MluI        MslI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        ScaI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

Acc65I       1930  GGTACC               G^GTACC
AcyI         2437  GGCGCC               G[GA]^CG[CT]C
AflII        1899  CTTAAG               C^TTAAG
AflIII       2393  ACATGT               A^C[GA][CT]GT
Alw44I       1588  GTGCAC               G^TGCAC
AlwNI        1433  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1588  GTGCAC               G^TGCAC
Asp700I       160  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1930  GGTACC               G^GTACC
AspI         1072  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1331  GGACC                G^G[AT]CC
BalI         1429  TGGCCA               TGG^CCA
BbeI         2437  GGCGCC               GGCGC^C
BbrPI         625  CACGTG               CAC^GTG
BbvCI        2577  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2361  CGAGAGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1899  CTTAAG               C^TTAAG
BsaAI         625  CACGTG               [CT]AC^GT[GA]
BsaHI        2437  GGCGCC               G[GA]^CG[CT]C
BsmI           55  GAATGC               GAATGC(1/-1)
BspLU11I     2393  ACATGT               A^CATGT
BsrBI        2517  CCGCTC               CCGCTC(-3/-3)
BsrFI        2590  GCCGGT               [GA]^CCGG[CT]
BssSI        1425  CTCGTG               CACGAG(-5/-1)
BstAPI        637  GCATCGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        965  GGTCACC              G^GT[ACGT]ACC
BstPI         965  GGTCACC              G^GT[ACGT]ACC
Cfr10I       2590  GCCGGT               [GA]^CCGG[CT]
DraII        2728  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1713  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2625  CCTCGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2728  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       965  GGTCACC              G^GT[ACGT]ACC
HaeII        2437  GGCGCC               [GA]GCGC^[CT]
HgaI         2170  GACGC                GACGC(5/10)
Hin1I        2437  GGCGCC               G[GA]^CG[CT]C
HincII       2317  GTCAAC               GT[CT]^[GA]AC
HindII       2317  GTCAAC               GT[CT]^[GA]AC
KasI         2437  GGCGCC               G^GCGCC
KpnI         1930  GGTACC               GGTAC^C
KspI            6  CCGCGG               CCGC^GG
MaeII         626  ACGT                 A^CGT
MluNI        1429  TGGCCA               TGG^CCA
MscI         1429  TGGCCA               TGG^CCA
NarI         2437  GGCGCC               GG^CGCC
NcoI         1335  CCATGG               C^CATGG
NspI         2393  ACATGT               [GA]CATG^[CT]
PaeR7I       1541  CTCGAG               C^TCGAG
PflFI        1072  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         625  CACGTG               CAC^GTG
PmlI          625  CACGTG               CAC^GTG
SacI          303  GAGCTC               GAGCT^C
SacII           6  CCGCGG               CCGC^GG
SapI           95  GAAGAGC              GCTCTTC(1/4)
SbfI         2496  CCTGCAGG             CCTGCA^GG
SfoI         2437  GGCGCC               GGC^GCC
SgrAI        2589  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI         2462  CCCGGG               CCC^GGG
Sse8387I     2496  CCTGCAGG             CCTGCA^GG
StuI         1003  AGGCCT               AGG^CCT
Tth111I      1072  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2072  CCAAAACCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1541  CTCGAG               C^TCGAG
XmaCI        2462  CCCGGG               C^CCGGG
XmaI         2462  CCCGGG               C^CCGGG
XmnI          160  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccIII       1079  TCCGGA               T^CCGGA
             1206  TCCGGA              
AlwI          673  GATCC                GGATC(4/5)
             1082  GGATC               
AocI         1053  CCTCAGG              CC^T[ACGT]AGG
             1380  CCTGAGG             
BglII         820  AGATCT               A^GATCT
             2009  AGATCT              
BsaWI        1079  TCCGGA               [AT]^CCGG[AT]
             1206  TCCGGA              
BseAI        1079  TCCGGA               T^CCGGA
             1206  TCCGGA              
BspEI        1079  TCCGGA               T^CCGGA
             1206  TCCGGA              
Bsu36I       1053  CCTCAGG              CC^T[ACGT]AGG
             1380  CCTGAGG             
EaeI         1429  TGGCCA               [CT]^GGCC[GA]
             1749  TGGCCG              
Eco81I       1053  CCTCAGG              CC^T[ACGT]AGG
             1380  CCTGAGG             
HindIII       766  AAGCTT               A^AGCTT
             1870  AAGCTT              
MroI         1079  TCCGGA               T^CCGGA
             1206  TCCGGA              
PflMI         987  CCAAAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1432  CCAGGAGCTGG         
PshAI        2025  GACCGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2182  GACCAGGGTC          
Van91I        987  CCAAAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1432  CCAGGAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        AspHI       AvaI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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