Restriction map for pF1KSDA0146
 (Linear) (Six-base) MAPSORT of: KIAA0146.seq  Check: 4962  from: 1  to: 2745



KIAA0146.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2504    2745 
   Size:       2504     241
 
Acc65I G'GTAC_C

Cuts at:      0    1930    2745 
   Size:       1930     815
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     221    1999    2138    2209    2745 
   Size:        158      63    1778     139      71     536

  Fragments arranged by size: 

                1778     536     158     139      71      63
 
AflII C'TTAA_G

Cuts at:      0    1899    2745 
   Size:       1899     846
 
AflIII A'CryG_T

Cuts at:      0    2393    2745 
   Size:       2393     352
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1643    1675    2198    2230    2745 
   Size:       1643      32     523      32     515

  Fragments arranged by size: 

                1643     523     515      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1438    2745 
   Size:       1438    1307
 
ApaLI G'TGCA_C

Cuts at:      0    1588    2745 
   Size:       1588    1157
 
ApoI r'AATT_y

Cuts at:      0     424     539     750     905    2745 
   Size:        424     115     211     155    1840

  Fragments arranged by size: 

                1840     424     211     155     115
 
AvaI C'yCGr_G

Cuts at:      0      20    1541    2462    2745 
   Size:         20    1521     921     283

  Fragments arranged by size: 

                1521     921     283      20
 
BanI G'GyrC_C

Cuts at:      0    1930    2437    2492    2745 
   Size:       1930     507      55     253

  Fragments arranged by size: 

                1930     507     253      55
 
BanII G_rGCy'C

Cuts at:      0      28     307    2116    2745 
   Size:         28     279    1809     629

  Fragments arranged by size: 

                1809     629     279      28
 
BbeI G_GCGC'C

Cuts at:      0    2441    2745 
   Size:       2441     304
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     214    1862    2435    2496    2745 
   Size:        214    1648     573      61     249

  Fragments arranged by size: 

                1648     573     249     214      61
 
BbvCI CC'TCA_GC

Cuts at:      0    2578    2745 
   Size:       2578     167
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2350    2384    2745 
   Size:       2350      34     361

  Fragments arranged by size: 

                2350     361      34
 
BglII A'GATC_T

Cuts at:      0     820    2009    2745 
   Size:        820    1189     736

  Fragments arranged by size: 

                1189     820     736
 
Bme1580I G_kGCm'C

Cuts at:      0    1343    1592    2745 
   Size:       1343     249    1153

  Fragments arranged by size: 

                1343    1153     249
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     390    2745 
   Size:        390    2355
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     121    1406    1927    2745 
   Size:        121    1285     521     818

  Fragments arranged by size: 

                1285     818     521     121
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     728    1028    1460    1778    1868    1897    2017 
   Size:        291     437     300     432     318      90      29     120

Cuts at:   2017    2146    2745 
   Size:        129     599

  Fragments arranged by size: 

                 599     437     432     318     300     291     129     120
                  90      29
 
Bpu10I CC'TnA_GC

Cuts at:      0     148    1515    2578    2745 
   Size:        148    1367    1063     167

  Fragments arranged by size: 

                1367    1063     167     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2470    2505    2745 
   Size:       2470      35     240

  Fragments arranged by size: 

                2470     240      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     688    2051    2253    2745 
   Size:        109     579    1363     202     492

  Fragments arranged by size: 

                1363     579     492     202     109
 
BsaAI yAC'GTr

Cuts at:      0     627    2745 
   Size:        627    2118
 
BsaHI Gr'CG_yC

Cuts at:      0    2438    2745 
   Size:       2438     307
 
BsaWI w'CCGG_w

Cuts at:      0    1079    1206    2745 
   Size:       1079     127    1539

  Fragments arranged by size: 

                1539    1079     127
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     294     324    1359    1389    1427    1433    1457    1463 
   Size:        294      30    1035      30      38       6      24       6

Cuts at:   1463    2745 
   Size:       1282

  Fragments arranged by size: 

                1282    1035     294      38      30      30      24       6
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     972    1781    2065    2745 
   Size:        972     809     284     680

  Fragments arranged by size: 

                 972     809     680     284
 
BseYI C'CCAG_C

Cuts at:      0    1477    1530    1725    2109    2137    2745 
   Size:       1477      53     195     384      28     608

  Fragments arranged by size: 

                1477     608     384     195      53      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649    2553    2741    2745 
   Size:        649    1904     188       4

  Fragments arranged by size: 

                1904     649     188       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     307    1592    2368    2745 
   Size:        307    1285     776     377

  Fragments arranged by size: 

                1285     776     377     307
 
BsmI GAATG_Cn'

Cuts at:      0      61    2745 
   Size:         61    2684
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     307    1343    1592    2116    2368    2745 
   Size:         28     279    1036     249     524     252     377

  Fragments arranged by size: 

                1036     524     377     279     252     249      28
 
BspEI T'CCGG_A

Cuts at:      0    1079    1206    2745 
   Size:       1079     127    1539

  Fragments arranged by size: 

                1539    1079     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78      91    2490    2504    2745 
   Size:         78      13    2399      14     241

  Fragments arranged by size: 

                2399     241      78      14      13
 
BsrBI CCG'CTC

Cuts at:      0    2519    2745 
   Size:       2519     226
 
BsrFI r'CCGG_y

Cuts at:      0    2590    2745 
   Size:       2590     155
 
BssSI C'ACGA_G

Cuts at:      0    1425    2745 
   Size:       1425    1320
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     643    2745 
   Size:        643    2102
 
BstEII G'GTnAC_C

Cuts at:      0     965    2745 
   Size:        965    1780
 
BstYI r'GATC_y

Cuts at:      0     672     820    1082    2009    2745 
   Size:        672     148     262     927     736

  Fragments arranged by size: 

                 927     736     672     262     148
 
Bsu36I CC'TnA_GG

Cuts at:      0    1054    1381    2745 
   Size:       1054     327    1364

  Fragments arranged by size: 

                1364    1054     327
 
BtgI C'CryG_G

Cuts at:      0       6    1335    1952    2745 
   Size:          6    1329     617     793

  Fragments arranged by size: 

                1329     793     617       6
 
BtsI GCAGTG_nn'

Cuts at:      0      82    2337    2366    2536    2745 
   Size:         82    2255      29     170     209

  Fragments arranged by size: 

                2255     209     170      82      29
 
DraIII CAC_nnn'GTG

Cuts at:      0    1718    2745 
   Size:       1718    1027
 
EaeI y'GGCC_r

Cuts at:      0    1429    1749    2745 
   Size:       1429     320     996

  Fragments arranged by size: 

                1429     996     320
 
EarI CTCTTCn'nnn_

Cuts at:      0      90     197    1033    1478    1763    1975    2164    2611 
   Size:         90     107     836     445     285     212     189     447

Cuts at:   2611    2745 
   Size:        134

  Fragments arranged by size: 

                 836     447     445     285     212     189     134     107
                  90
 
EcoICRI GAG'CTC

Cuts at:      0     305    2745 
   Size:        305    2440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     221     291     728    1028    1460    1778    1868 
   Size:        158      63      70     437     300     432     318      90

Cuts at:   1868    1897    1999    2017    2138    2146    2209    2745 
   Size:         29     102      18     121       8      63     536

  Fragments arranged by size: 

                 536     437     432     318     300     158     121     102
                  90      70      63      63      29      18       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2629    2745 
   Size:       2629     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2729    2745 
   Size:       2729      16
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1253    2745 
   Size:       1253    1492
 
HaeII r_GCGC'y

Cuts at:      0    2441    2745 
   Size:       2441     304
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     330     362     493     525     538     570     592     624 
   Size:        330      32     131      32      13      32      22      32

Cuts at:    624    1074    1106    1427    1459    1764    1796    1908    1940 
   Size:        450      32     321      32     305      32     112      32

Cuts at:   1940    2503    2528    2535    2560    2745 
   Size:        563      25       7      25     185

  Fragments arranged by size: 

                 563     450     330     321     305     185     131     112
                  32      32      32      32      32      32      32      32
                  25      25      22      13       7
 
HincII GTy'rAC

Cuts at:      0    2319    2745 
   Size:       2319     426
 
HindIII A'AGCT_T

Cuts at:      0     766    1870    2745 
   Size:        766    1104     875

  Fragments arranged by size: 

                1104     875     766
 
KasI G'GCGC_C

Cuts at:      0    2437    2745 
   Size:       2437     308
 
KpnI G_GTAC'C

Cuts at:      0    1934    2745 
   Size:       1934     811
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     305     491    1604    2170    2745 
   Size:         25     280     186    1113     566     575

  Fragments arranged by size: 

                1113     575     566     280     186      25
 
MscI TGG'CCA

Cuts at:      0    1431    2745 
   Size:       1431    1314
 
MspA1I CmG'CkG

Cuts at:      0       8     124     199    1534    1690    1895    1977    2687 
   Size:          8     116      75    1335     156     205      82     710

Cuts at:   2687    2745 
   Size:         58

  Fragments arranged by size: 

                1335     710     205     156     116      82      75      58
                   8
 
NarI GG'CG_CC

Cuts at:      0    2438    2745 
   Size:       2438     307
 
NcoI C'CATG_G

Cuts at:      0    1335    2745 
   Size:       1335    1410
 
NspI r_CATG'y

Cuts at:      0    2397    2745 
   Size:       2397     348
 
PciI A'CATG_T

Cuts at:      0    2393    2745 
   Size:       2393     352
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     993    1438    2745 
   Size:        993     445    1307

  Fragments arranged by size: 

                1307     993     445
 
PfoI T'CCnGG_A

Cuts at:      0      69    1394    2123    2507    2745 
   Size:         69    1325     729     384     238

  Fragments arranged by size: 

                1325     729     384     238      69
 
PmlI CAC'GTG

Cuts at:      0     627    2745 
   Size:        627    2118
 
PshAI GACnn'nnGTC

Cuts at:      0    2029    2186    2745 
   Size:       2029     157     559

  Fragments arranged by size: 

                2029     559     157
 
PsiI TTA'TAA

Cuts at:      0    1868    2745 
   Size:       1868     877
 
PspXI vC'TCGA_Gb

Cuts at:      0    1541    2745 
   Size:       1541    1204
 
PstI C_TGCA'G

Cuts at:      0      89     809    1047    2332    2501    2739    2745 
   Size:         89     720     238    1285     169     238       6

  Fragments arranged by size: 

                1285     720     238     238     169      89       6
 
PvuII CAG'CTG

Cuts at:      0     124     199    1534    1977    2687    2745 
   Size:        124      75    1335     443     710      58

  Fragments arranged by size: 

                1335     710     443     124      75      58
 
SacI G_AGCT'C

Cuts at:      0     307    2745 
   Size:        307    2438
 
SacII CC_GC'GG

Cuts at:      0       9    2745 
   Size:          9    2736
 
SapI GCTCTTCn'nnn_

Cuts at:      0      90    2745 
   Size:         90    2655
 
SbfI CC_TGCA'GG

Cuts at:      0    2501    2745 
   Size:       2501     244
 
SfcI C'TryA_G

Cuts at:      0      85     533     805    1043    1316    1663    2328    2497 
   Size:         85     448     272     238     273     347     665     169

Cuts at:   2497    2735    2745 
   Size:        238      10

  Fragments arranged by size: 

                 665     448     347     273     272     238     238     169
                  85      10
 
SfoI GGC'GCC

Cuts at:      0    2439    2745 
   Size:       2439     306
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2590    2745 
   Size:       2590     155
 
SmaI CCC'GGG

Cuts at:      0    2464    2745 
   Size:       2464     281
 
SmlI C'TyrA_G

Cuts at:      0    1541    1899    2485    2520    2745 
   Size:       1541     358     586      35     225

  Fragments arranged by size: 

                1541     586     358     225      35
 
StuI AGG'CCT

Cuts at:      0    1005    2745 
   Size:       1005    1740
 
StyI C'CwwG_G

Cuts at:      0     676    1034    1335    2745 
   Size:        676     358     301    1410

  Fragments arranged by size: 

                1410     676     358     301
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2041    2745 
   Size:       2041     704
 
TatI w'GTAC_w

Cuts at:      0    1199    2745 
   Size:       1199    1546
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     294     326    1271    1303    1433    1465    1806    1838 
   Size:        294      32     945      32     130      32     341      32

Cuts at:   1838    2184    2216    2621    2653    2745 
   Size:        346      32     405      32      92

  Fragments arranged by size: 

                 945     405     346     341     294     130      92      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1075    2745 
   Size:       1075    1670
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2079    2745 
   Size:       2079     666
 
XhoI C'TCGA_G

Cuts at:      0    1541    2745 
   Size:       1541    1204
 
XmaI C'CCGG_G

Cuts at:      0    2462    2745 
   Size:       2462     283
 
XmnI GAAnn'nnTTC

Cuts at:      0     164    2745 
   Size:        164    2581
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII   AflIII     AloI    AlwNI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI   MspA1I     NarI     NcoI
     NspI     PciI    PflMI     PfoI     PmlI    PshAI     PsiI    PspXI
     PstI    PvuII     SacI    SacII     SapI     SbfI     SfcI     SfoI
    SgrAI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI     AlfI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaBI    BsiEI
    BsiWI    BsmBI    BspHI    BsrDI    BsrGI   BssHII    BstBI    BstXI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     MfeI     MluI
     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PpiI    PpuMI   PspOMI     PsrI     PvuI
    RsrII     SalI    SanDI     ScaI    SexAI     SfiI     SgfI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     ZraI
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