Restriction map for pF1KSDA0096
 (Linear) (Six-base) MAPSORT of: KIAA0096.seq  Check: 598  from: 1  to: 2295



KIAA0096.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     588    2295 
   Size:        588    1707
 
Acc65I G'GTAC_C

Cuts at:      0    1861    2295 
   Size:       1861     434
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    2295 
   Size:        790    1505
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1912    2111    2295 
   Size:       1912     199     184

  Fragments arranged by size: 

                1912     199     184
 
ApaI G_GGCC'C

Cuts at:      0    1718    2295 
   Size:       1718     577
 
ApoI r'AATT_y

Cuts at:      0     490     551    2295 
   Size:        490      61    1744

  Fragments arranged by size: 

                1744     490      61
 
AvaI C'yCGr_G

Cuts at:      0    1053    1175    1833    2295 
   Size:       1053     122     658     462

  Fragments arranged by size: 

                1053     658     462     122
 
BamHI G'GATC_C

Cuts at:      0    1004    2295 
   Size:       1004    1291
 
BanI G'GyrC_C

Cuts at:      0    1278    1691    1861    2295 
   Size:       1278     413     170     434

  Fragments arranged by size: 

                1278     434     413     170
 
BanII G_rGCy'C

Cuts at:      0    1606    1718    1928    2295 
   Size:       1606     112     210     367

  Fragments arranged by size: 

                1606     367     210     112
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     344     429    2295 
   Size:        344      85    1866

  Fragments arranged by size: 

                1866     344      85
 
BclI T'GATC_A

Cuts at:      0     677    2295 
   Size:        677    1618
 
BfrBI ATG'CAT

Cuts at:      0     197    2295 
   Size:        197    2098
 
BglII A'GATC_T

Cuts at:      0    1042    1463    2295 
   Size:       1042     421     832

  Fragments arranged by size: 

                1042     832     421
 
Bme1580I G_kGCm'C

Cuts at:      0    1718    2295 
   Size:       1718     577
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1065    1787    2295 
   Size:       1065     722     508

  Fragments arranged by size: 

                1065     722     508
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1538    1590    1809    2295 
   Size:        530    1008      52     219     486

  Fragments arranged by size: 

                1008     530     486     219      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    2295 
   Size:       1138    1157
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1618    2295 
   Size:       1618     677
 
BsaAI yAC'GTr

Cuts at:      0    2051    2295 
   Size:       2051     244
 
BsaBI GATnn'nnATC

Cuts at:      0    1508    2295 
   Size:       1508     787
 
BsaHI Gr'CG_yC

Cuts at:      0     932    2295 
   Size:        932    1363
 
BsaWI w'CCGG_w

Cuts at:      0    1580    2295 
   Size:       1580     715
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1122    1152    1575    1605    2295 
   Size:       1122      30     423      30     690

  Fragments arranged by size: 

                1122     690     423      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     305     337     892    1372    2295 
   Size:        305      32     555     480     923

  Fragments arranged by size: 

                 923     555     480     305      32
 
BseYI C'CCAG_C

Cuts at:      0    1885    1918    1967    2271    2295 
   Size:       1885      33      49     304      24

  Fragments arranged by size: 

                1885     304      49      33      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229    2295 
   Size:        229    2066
 
BsiHKAI G_wGCw'C

Cuts at:      0     884    1928    1984    2295 
   Size:        884    1044      56     311

  Fragments arranged by size: 

                1044     884     311      56
 
BsmI GAATG_Cn'

Cuts at:      0    2085    2295 
   Size:       2085     210
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     923    2295 
   Size:        923    1372
 
Bsp1286I G_dGCh'C

Cuts at:      0     884    1606    1718    1928    1984    2295 
   Size:        884     722     112     210      56     311

  Fragments arranged by size: 

                 884     722     311     210     112      56
 
BspHI T'CATG_A

Cuts at:      0    1946    2295 
   Size:       1946     349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     588    1520    2079    2295 
   Size:        588     932     559     216

  Fragments arranged by size: 

                 932     588     559     216
 
BsrBI CCG'CTC

Cuts at:      0     544    2295 
   Size:        544    1751
 
BsrDI GCAATG_nn'

Cuts at:      0    1767    2295 
   Size:       1767     528
 
BsrFI r'CCGG_y

Cuts at:      0    1649    1879    2295 
   Size:       1649     230     416

  Fragments arranged by size: 

                1649     416     230
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1902    1975    2151    2295 
   Size:       1902      73     176     144

  Fragments arranged by size: 

                1902     176     144      73
 
BstYI r'GATC_y

Cuts at:      0     526     756    1004    1042    1463    2295 
   Size:        526     230     248      38     421     832

  Fragments arranged by size: 

                 832     526     421     248     230      38
 
Bsu36I CC'TnA_GG

Cuts at:      0    2243    2295 
   Size:       2243      52
 
BtgI C'CryG_G

Cuts at:      0      83    2295 
   Size:         83    2212
 
BtsI GCAGTG_nn'

Cuts at:      0    1731    1920    2105    2295 
   Size:       1731     189     185     190

  Fragments arranged by size: 

                1731     190     189     185
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1373    1408    1749    1784    1791    1826    2295 
   Size:       1373      35     341      35       7      35     469

  Fragments arranged by size: 

                1373     469     341      35      35      35       7
 
EaeI y'GGCC_r

Cuts at:      0     344    1087    1133    1200    2295 
   Size:        344     743      46      67    1095

  Fragments arranged by size: 

                1095     743     344      67      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     776     869    1340    1435    2295 
   Size:        776      93     471      95     860

  Fragments arranged by size: 

                 860     776     471      95      93
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     217    1329    2295 
   Size:        217    1112     966

  Fragments arranged by size: 

                1112     966     217
 
EcoICRI GAG'CTC

Cuts at:      0    1926    2295 
   Size:       1926     369
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     790    1538    1590    1809    2295 
   Size:        530     260     748      52     219     486

  Fragments arranged by size: 

                 748     530     486     260     219      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1113    1714    1715    2091    2295 
   Size:       1113     601       1     376     204

  Fragments arranged by size: 

                1113     601     376     204       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     791    2295 
   Size:        791    1504
 
FspI TGC'GCA

Cuts at:      0    2262    2295 
   Size:       2262      33
 
FspAI rTGC'GCAy

Cuts at:      0    2262    2295 
   Size:       2262      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     777     809    1146    1178    1253    1285    1575    1607 
   Size:        777      32     337      32      75      32     290      32

Cuts at:   1607    2295 
   Size:        688

  Fragments arranged by size: 

                 777     688     337     290      75      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     467    2295 
   Size:        467    1828
 
HindIII A'AGCT_T

Cuts at:      0    1290    2295 
   Size:       1290    1005
 
KpnI G_GTAC'C

Cuts at:      0    1865    2295 
   Size:       1865     430
 
MscI TGG'CCA

Cuts at:      0     346    1089    1202    2295 
   Size:        346     743     113    1093

  Fragments arranged by size: 

                1093     743     346     113
 
MslI CAynn'nnrTG

Cuts at:      0     622     742    2221    2295 
   Size:        622     120    1479      74

  Fragments arranged by size: 

                1479     622     120      74
 
MspA1I CmG'CkG

Cuts at:      0    1610    1829    1874    1902    2235    2279    2295 
   Size:       1610     219      45      28     333      44      16

  Fragments arranged by size: 

                1610     333     219      45      44      28      16
 
NaeI GCC'GGC

Cuts at:      0    1651    1881    2295 
   Size:       1651     230     414

  Fragments arranged by size: 

                1651     414     230
 
NgoMIV G'CCGG_C

Cuts at:      0    1649    1879    2295 
   Size:       1649     230     416

  Fragments arranged by size: 

                1649     416     230
 
NsiI A_TGCA'T

Cuts at:      0     199    2295 
   Size:        199    2096
 
NspI r_CATG'y

Cuts at:      0     106    2295 
   Size:        106    2189
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2008    2295 
   Size:       2008     287
 
PfoI T'CCnGG_A

Cuts at:      0     182    1435    2295 
   Size:        182    1253     860

  Fragments arranged by size: 

                1253     860     182
 
PpuMI rG'GwC_Cy

Cuts at:      0    1113    2091    2295 
   Size:       1113     978     204

  Fragments arranged by size: 

                1113     978     204
 
PspOMI G'GGCC_C

Cuts at:      0    1714    2295 
   Size:       1714     581
 
PstI C_TGCA'G

Cuts at:      0     585    1615    2100    2195    2295 
   Size:        585    1030     485      95     100

  Fragments arranged by size: 

                1030     585     485     100      95
 
PvuII CAG'CTG

Cuts at:      0    1610    1902    2279    2295 
   Size:       1610     292     377      16

  Fragments arranged by size: 

                1610     377     292      16
 
SacI G_AGCT'C

Cuts at:      0    1928    2295 
   Size:       1928     367
 
SfcI C'TryA_G

Cuts at:      0     581     748    1611    2096    2191    2295 
   Size:        581     167     863     485      95     104

  Fragments arranged by size: 

                 863     581     485     167     104      95
 
SmlI C'TyrA_G

Cuts at:      0    1117    2295 
   Size:       1117    1178
 
SpeI A'CTAG_T

Cuts at:      0     204    2295 
   Size:        204    2091
 
StuI AGG'CCT

Cuts at:      0    1217    2295 
   Size:       1217    1078
 
StyI C'CwwG_G

Cuts at:      0      62     799    2295 
   Size:         62     737    1496

  Fragments arranged by size: 

                1496     737      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     265    2295 
   Size:        265    2030
 
TatI w'GTAC_w

Cuts at:      0    1988    2295 
   Size:       1988     307
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1122    1154    2295 
   Size:       1122      32    1141

  Fragments arranged by size: 

                1141    1122      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1189    2295 
   Size:       1189    1106
 
XbaI T'CTAG_A

Cuts at:      0     273    2295 
   Size:        273    2022
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1773    2148    2295 
   Size:       1773     375     147

  Fragments arranged by size: 

                1773     375     147
 
XmnI GAAnn'nnTTC

Cuts at:      0     771    2295 
   Size:        771    1524
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BclI    BfrBI    BglII Bme1580I     BmrI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI     FspI    FspAI    Hin4I   HincII  HindIII     KpnI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BsiEI    BsiWI    BspEI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI    HaeII
     HpaI     KasI     MfeI     MluI     MmeI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
     XhoI     XmaI     ZraI
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