Restriction map for pF1KSDA0084
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbeI        
BcgI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       CfoI        
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HhaI        
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         591  CGCG                 CG^CG
AcsI          469  AAATTC               [GA]^AATT[CT]
AflIII        590  ACGCGT               A^C[GA][CT]GT
AocI           76  CCTCAGG              CC^T[ACGT]AGG
ApoI          469  AAATTC               [GA]^AATT[CT]
AspI         1292  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          921  TGGCCA               TGG^CCA
BanI          690  GGTGCC               G^G[CT][GA]CC
BbrPI         578  CACGTG               CAC^GTG
BciVI        1261  GTATCC               GTATCC(6/5)
BclI          314  TGATCA               T^GATCA
BcnI          613  CCGGG                CC^[GC]GG
BsaI         1290  GAGACC               GGTCTC(1/5)
BseRI        1516  GAGGAG               GAGGAG(10/8)
BspMI         191  ACCTGC               ACCTGC(4/8)
BsrFI         875  GCCGGC               [GA]^CCGG[CT]
BstUI         591  CGCG                 CG^CG
Bsu36I         76  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        875  GCCGGC               [GA]^CCGG[CT]
EaeI          921  TGGCCA               [CT]^GGCC[GA]
Eco81I         76  CCTCAGG              CC^T[ACGT]AGG
FbaI          314  TGATCA               T^GATCA
MluI          590  ACGCGT               A^CGCGT
MluNI         921  TGGCCA               TGG^CCA
MscI          921  TGGCCA               TGG^CCA
MseI          434  TTAA                 T^TAA
MspA1I       1555  CAGCTG               C[AC]G^C[GT]G
MvnI          591  CGCG                 CG^CG
NaeI          875  GCCGGC               GCC^GGC
NciI          613  CCGGG                CC^[GC]GG
NgoMIV        875  GCCGGC               G^CCGGC
NspI          989  GCATGC               [GA]CATG^[CT]
PflFI        1292  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         201  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         578  CACGTG               CAC^GTG
PmlI          578  CACGTG               CAC^GTG
PpuMI         157  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        1555  CAGCTG               CAG^CTG
SacI          148  GAGCTC               GAGCT^C
SphI          989  GCATGC               GCATG^C
SspI           52  AATATT               AAT^ATT
StuI           73  AGGCCT               AGG^CCT
Tru9I         434  TTAA                 T^TAA
Tth111I      1292  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        201  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          534  GTCTAC               GT^[AC][GT]AC
             1298  GTCTAC              
AccIII        721  TCCGGA               T^CCGGA
              977  TCCGGA              
AcyI          282  GACGCC               G[GA]^CG[CT]C
              943  GACGCC              
AlwI          441  GATCC                GGATC(4/5)
              585  GGATC               
AlwNI         202  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              460  CAGGGCCTG           
ApaI         1527  GGGCCC               GGGCC^C
             1634  GGGCCC              
BbvCI         161  CCTCAGC              CCTCAGC(-5/-2)
             1696  GCTGAGG             
BglII         331  AGATCT               A^GATCT
              833  AGATCT              
BsaAI         578  CACGTG               [CT]AC^GT[GA]
             1168  TACGTG              
BsaHI         282  GACGCC               G[GA]^CG[CT]C
              943  GACGCC              
BseAI         721  TCCGGA               T^CCGGA
              977  TCCGGA              
BspEI         721  TCCGGA               T^CCGGA
              977  TCCGGA              
BsrBI         271  GAGCGG               CCGCTC(-3/-3)
              933  GAGCGG              
EcoT14I       794  CCTTGG               C^C[AT][AT]GG
             1014  CCTTGG              
FauI          196  CCCGC                CCCGC(4/6)
              273  GCGGG               
Hin1I         282  GACGCC               G[GA]^CG[CT]C
              943  GACGCC              
MroI          721  TCCGGA               T^CCGGA
              977  TCCGGA              
MslI          990  CATGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1212  CACCTGTGTG          
PleI          132  GACTC                GAGTC(4/5)
             1508  GAGTC               
SapI          808  GAAGAGC              GCTCTTC(1/4)
              858  GAAGAGC             
StyI          794  CCTTGG               C^C[AT][AT]GG
             1014  CCTTGG              
TaqI         1151  TCGA                 T^CGA
             1327  TCGA                
Tsp509I       374  AATT                 ^AATT
              470  AATT                
TthHB8I      1151  TCGA                 T^CGA
             1327  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BanII       BbsI        BbvI        BmyI        BpmI        
BpuAI       BsaJI       BsaWI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        TseI        Tsp45I      TspRI       XhoII       
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