Restriction map for pF1KSDA0084
 (Linear) (Six-base) MAPSORT of: KIAA0084.seq  Check: 8024  from: 1  to: 1734



KIAA0084.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0     535    1299    1734 
   Size:        535     764     435

  Fragments arranged by size: 

                 764     535     435
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1270    1734 
   Size:       1270     464
 
AflIII A'CryG_T

Cuts at:      0     590    1734 
   Size:        590    1144
 
AleI CACnn'nnGTG

Cuts at:      0    1216    1734 
   Size:       1216     518
 
AlwNI CAG_nnn'CTG

Cuts at:      0     207     465    1734 
   Size:        207     258    1269

  Fragments arranged by size: 

                1269     258     207
 
ApaI G_GGCC'C

Cuts at:      0    1531    1638    1734 
   Size:       1531     107      96

  Fragments arranged by size: 

                1531     107      96
 
ApoI r'AATT_y

Cuts at:      0     469    1734 
   Size:        469    1265
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     475     508    1734 
   Size:        475      33    1226

  Fragments arranged by size: 

                1226     475      33
 
BanI G'GyrC_C

Cuts at:      0     690    1734 
   Size:        690    1044
 
BanII G_rGCy'C

Cuts at:      0     152     668    1531    1638    1734 
   Size:        152     516     863     107      96

  Fragments arranged by size: 

                 863     516     152     107      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     286     999    1445    1451    1734 
   Size:        286     713     446       6     283

  Fragments arranged by size: 

                 713     446     286     283       6
 
BbvCI CC'TCA_GC

Cuts at:      0     162    1697    1734 
   Size:        162    1535      37

  Fragments arranged by size: 

                1535     162      37
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1272    1734 
   Size:       1272     462
 
BclI T'GATC_A

Cuts at:      0     314    1734 
   Size:        314    1420
 
BglII A'GATC_T

Cuts at:      0     331     833    1734 
   Size:        331     502     901

  Fragments arranged by size: 

                 901     502     331
 
Bme1580I G_kGCm'C

Cuts at:      0     695    1175    1531    1638    1661    1734 
   Size:        695     480     356     107      23      73

  Fragments arranged by size: 

                 695     480     356     107      73      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     629    1664    1734 
   Size:        629    1035      70

  Fragments arranged by size: 

                1035     629      70
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     687     834    1734 
   Size:        687     147     900

  Fragments arranged by size: 

                 900     687     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     229     310     619    1332    1484    1691    1734 
   Size:        142      87      81     309     713     152     207      43

  Fragments arranged by size: 

                 713     309     207     152     142      87      81      43
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     162     672    1697    1734 
   Size:        162     510    1025      37

  Fragments arranged by size: 

                1025     510     162      37
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1284    1734 
   Size:       1284     450
 
BsaAI yAC'GTr

Cuts at:      0     580    1170    1734 
   Size:        580     590     564

  Fragments arranged by size: 

                 590     580     564
 
BsaHI Gr'CG_yC

Cuts at:      0     283     944    1734 
   Size:        283     661     790

  Fragments arranged by size: 

                 790     661     283
 
BsaWI w'CCGG_w

Cuts at:      0     528     721     780     977    1734 
   Size:        528     193      59     197     757

  Fragments arranged by size: 

                 757     528     197     193      59
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1531    1734 
   Size:       1531     203
 
BseYI C'CCAG_C

Cuts at:      0     200     643     667     709    1734 
   Size:        200     443      24      42    1025

  Fragments arranged by size: 

                1025     443     200      42      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     277     651    1177    1627    1734 
   Size:        229      48     374     526     450     107

  Fragments arranged by size: 

                 526     450     374     229     107      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     152     296     528     639     962    1734 
   Size:        152     144     232     111     323     772

  Fragments arranged by size: 

                 772     323     232     152     144     111
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     296     528     639     668     695     962    1175 
   Size:        152     144     232     111      29      27     267     213

Cuts at:   1175    1531    1638    1661    1734 
   Size:        356     107      23      73

  Fragments arranged by size: 

                 356     267     232     213     152     144     111     107
                  73      29      27      23
 
BspEI T'CCGG_A

Cuts at:      0     721     977    1734 
   Size:        721     256     757

  Fragments arranged by size: 

                 757     721     256
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     200    1734 
   Size:        200    1534
 
BsrBI CCG'CTC

Cuts at:      0     273     935    1734 
   Size:        273     662     799

  Fragments arranged by size: 

                 799     662     273
 
BsrFI r'CCGG_y

Cuts at:      0     875    1734 
   Size:        875     859
 
BstYI r'GATC_y

Cuts at:      0     331     440     833    1734 
   Size:        331     109     393     901

  Fragments arranged by size: 

                 901     393     331     109
 
Bsu36I CC'TnA_GG

Cuts at:      0      77    1734 
   Size:         77    1657
 
BtsI GCAGTG_nn'

Cuts at:      0     173     528     639    1734 
   Size:        173     355     111    1095

  Fragments arranged by size: 

                1095     355     173     111
 
EaeI y'GGCC_r

Cuts at:      0     921    1734 
   Size:        921     813
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     803     853    1734 
   Size:         64     739      50     881

  Fragments arranged by size: 

                 881     739      64      50
 
EcoICRI GAG'CTC

Cuts at:      0     150    1734 
   Size:        150    1584
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     229     310     619    1270    1332    1484    1691 
   Size:        142      87      81     309     651      62     152     207

Cuts at:   1691    1734 
   Size:         43

  Fragments arranged by size: 

                 651     309     207     152     142      87      81      62
                  43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     158     462     676    1527    1528    1634    1635    1734 
   Size:        158     304     214     851       1     106       1      99

  Fragments arranged by size: 

                 851     304     214     158     106      99       1       1
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    1046    1078    1281    1313    1734 
   Size:        577      32     437      32     203      32     421

  Fragments arranged by size: 

                 577     437     421     203      32      32      32
 
MluI A'CGCG_T

Cuts at:      0     590    1734 
   Size:        590    1144
 
MscI TGG'CCA

Cuts at:      0     923    1734 
   Size:        923     811
 
MslI CAynn'nnrTG

Cuts at:      0     994    1216    1734 
   Size:        994     222     518

  Fragments arranged by size: 

                 994     518     222
 
MspA1I CmG'CkG

Cuts at:      0    1557    1734 
   Size:       1557     177
 
NaeI GCC'GGC

Cuts at:      0     877    1734 
   Size:        877     857
 
NgoMIV G'CCGG_C

Cuts at:      0     875    1734 
   Size:        875     859
 
NspI r_CATG'y

Cuts at:      0     993    1734 
   Size:        993     741
 
PasI CC'CwG_GG

Cuts at:      0     154    1734 
   Size:        154    1580
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     207    1734 
   Size:        207    1527
 
PfoI T'CCnGG_A

Cuts at:      0     119     206     443    1139    1668    1734 
   Size:        119      87     237     696     529      66

  Fragments arranged by size: 

                 696     529     237     119      87      66
 
PmlI CAC'GTG

Cuts at:      0     580    1734 
   Size:        580    1154
 
PpuMI rG'GwC_Cy

Cuts at:      0     158    1734 
   Size:        158    1576
 
PspOMI G'GGCC_C

Cuts at:      0    1527    1634    1734 
   Size:       1527     107     100

  Fragments arranged by size: 

                1527     107     100
 
PvuII CAG'CTG

Cuts at:      0    1557    1734 
   Size:       1557     177
 
SacI G_AGCT'C

Cuts at:      0     152    1734 
   Size:        152    1582
 
SapI GCTCTTCn'nnn_

Cuts at:      0     803     853    1734 
   Size:        803      50     881

  Fragments arranged by size: 

                 881     803      50
 
SphI G_CATG'C

Cuts at:      0     993    1734 
   Size:        993     741
 
SspI AAT'ATT

Cuts at:      0      54    1734 
   Size:         54    1680
 
StuI AGG'CCT

Cuts at:      0      75    1734 
   Size:         75    1659
 
StyI C'CwwG_G

Cuts at:      0     794    1014    1734 
   Size:        794     220     720

  Fragments arranged by size: 

                 794     720     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     280    1576    1734 
   Size:        280    1296     158

  Fragments arranged by size: 

                1296     280     158
 
Tth111I GACn'n_nGTC

Cuts at:      0    1295    1734 
   Size:       1295     439
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApaI     ApoI     BaeI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BglII Bme1580I
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI    BstYI   Bsu36I     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    Hin4I     MluI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PvuII
     SacI     SapI     SphI     SspI     StuI     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BbeI     BcgI    BfrBI     BglI     BlpI    BmgBI     BmtI
    BpuEI    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SwaI     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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