Restriction map for pF1KSDA0064
 (Linear) (Six-base) MAPSORT of: KIAA0064.seq  Check: 3018  from: 1  to: 1410



KIAA0064.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389    1410 
   Size:        389    1021
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1141    1410 
   Size:       1141     269
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     795    1410 
   Size:        120     675     615

  Fragments arranged by size: 

                 675     615     120
 
AvaI C'yCGr_G

Cuts at:      0    1274    1410 
   Size:       1274     136
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     465     498    1410 
   Size:        465      33     912

  Fragments arranged by size: 

                 912     465      33
 
BanII G_rGCy'C

Cuts at:      0     890    1410 
   Size:        890     520
 
BbvCI CC'TCA_GC

Cuts at:      0     209    1410 
   Size:        209    1201
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     238     272    1410 
   Size:        238      34    1138

  Fragments arranged by size: 

                1138     238      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     466    1410 
   Size:        466     944
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61     832     911    1410 
   Size:         61     771      79     499

  Fragments arranged by size: 

                 771     499      79      61
 
BlpI GC'TnA_GC

Cuts at:      0     367     889    1410 
   Size:        367     522     521

  Fragments arranged by size: 

                 522     521     367
 
Bme1580I G_kGCm'C

Cuts at:      0     315    1410 
   Size:        315    1095
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     983    1410 
   Size:        983     427
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     777    1410 
   Size:        777     633
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1410 
   Size:        448     962
 
Bpu10I CC'TnA_GC

Cuts at:      0     209     601    1410 
   Size:        209     392     809

  Fragments arranged by size: 

                 809     392     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1276    1281    1410 
   Size:       1276       5     129

  Fragments arranged by size: 

                1276     129       5
 
BsaAI yAC'GTr

Cuts at:      0      60      78    1410 
   Size:         60      18    1332

  Fragments arranged by size: 

                1332      60      18
 
BsaBI GATnn'nnATC

Cuts at:      0    1117    1403    1410 
   Size:       1117     286       7

  Fragments arranged by size: 

                1117     286       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     237     963    1410 
   Size:        237     726     447

  Fragments arranged by size: 

                 726     447     237
 
BseYI C'CCAG_C

Cuts at:      0    1339    1410 
   Size:       1339      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     112    1410 
   Size:         76      36    1298

  Fragments arranged by size: 

                1298      76      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     890    1410 
   Size:        890     520
 
BsmI GAATG_Cn'

Cuts at:      0     172    1410 
   Size:        172    1238
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1206    1410 
   Size:       1206     204
 
Bsp1286I G_dGCh'C

Cuts at:      0     315     890    1410 
   Size:        315     575     520

  Fragments arranged by size: 

                 575     520     315
 
BspHI T'CATG_A

Cuts at:      0    1058    1109    1410 
   Size:       1058      51     301

  Fragments arranged by size: 

                1058     301      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     321     674    1410 
   Size:        321     353     736

  Fragments arranged by size: 

                 736     353     321
 
BsrDI GCAATG_nn'

Cuts at:      0     268     982    1410 
   Size:        268     714     428

  Fragments arranged by size: 

                 714     428     268
 
BsrFI r'CCGG_y

Cuts at:      0     731    1070    1190    1410 
   Size:        731     339     120     220

  Fragments arranged by size: 

                 731     339     220     120
 
BssSI C'ACGA_G

Cuts at:      0     130     999    1410 
   Size:        130     869     411

  Fragments arranged by size: 

                 869     411     130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     956    1410 
   Size:        956     454
 
BstEII G'GTnAC_C

Cuts at:      0     714     942     966    1083    1410 
   Size:        714     228      24     117     327

  Fragments arranged by size: 

                 714     327     228     117      24
 
BstYI r'GATC_y

Cuts at:      0     450     707    1404    1410 
   Size:        450     257     697       6

  Fragments arranged by size: 

                 697     450     257       6
 
BtgI C'CryG_G

Cuts at:      0    1133    1368    1410 
   Size:       1133     235      42

  Fragments arranged by size: 

                1133     235      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     969    1410 
   Size:        969     441
 
BtsI GCAGTG_nn'

Cuts at:      0    1241    1249    1410 
   Size:       1241       8     161

  Fragments arranged by size: 

                1241     161       8
 
EaeI y'GGCC_r

Cuts at:      0     912    1410 
   Size:        912     498
 
EarI CTCTTCn'nnn_

Cuts at:      0     601    1410 
   Size:        601     809
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42     892    1410 
   Size:         42     850     518

  Fragments arranged by size: 

                 850     518      42
 
EcoICRI GAG'CTC

Cuts at:      0     888    1410 
   Size:        888     522
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     448    1410 
   Size:        389      59     962

  Fragments arranged by size: 

                 962     389      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0     333    1410 
   Size:        333    1077
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366    1410 
   Size:        334      32    1044

  Fragments arranged by size: 

                1044     334      32
 
HincII GTy'rAC

Cuts at:      0     393    1410 
   Size:        393    1017
 
HindIII A'AGCT_T

Cuts at:      0     187    1410 
   Size:        187    1223
 
MscI TGG'CCA

Cuts at:      0     914    1410 
   Size:        914     496
 
MslI CAynn'nnrTG

Cuts at:      0      76      80     949    1410 
   Size:         76       4     869     461

  Fragments arranged by size: 

                 869     461      76       4
 
MspA1I CmG'CkG

Cuts at:      0      35      44    1410 
   Size:         35       9    1366

  Fragments arranged by size: 

                1366      35       9
 
NaeI GCC'GGC

Cuts at:      0    1192    1410 
   Size:       1192     218
 
NcoI C'CATG_G

Cuts at:      0    1133    1410 
   Size:       1133     277
 
NgoMIV G'CCGG_C

Cuts at:      0    1190    1410 
   Size:       1190     220
 
NspI r_CATG'y

Cuts at:      0     249     954    1410 
   Size:        249     705     456

  Fragments arranged by size: 

                 705     456     249
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     274    1410 
   Size:        120     154    1136

  Fragments arranged by size: 

                1136     154     120
 
PfoI T'CCnGG_A

Cuts at:      0     425    1410 
   Size:        425     985
 
PmlI CAC'GTG

Cuts at:      0      78    1410 
   Size:         78    1332
 
PpuMI rG'GwC_Cy

Cuts at:      0     333    1410 
   Size:        333    1077
 
PstI C_TGCA'G

Cuts at:      0     899    1410 
   Size:        899     511
 
SacI G_AGCT'C

Cuts at:      0     890    1410 
   Size:        890     520
 
SfcI C'TryA_G

Cuts at:      0     108     434     895    1075    1410 
   Size:        108     326     461     180     335

  Fragments arranged by size: 

                 461     335     326     180     108
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1190    1410 
   Size:       1190     220
 
SmaI CCC'GGG

Cuts at:      0    1276    1410 
   Size:       1276     134
 
SmlI C'TyrA_G

Cuts at:      0    1255    1296    1410 
   Size:       1255      41     114

  Fragments arranged by size: 

                1255     114      41
 
SphI G_CATG'C

Cuts at:      0     954    1410 
   Size:        954     456
 
StyI C'CwwG_G

Cuts at:      0     353     719    1133    1410 
   Size:        353     366     414     277

  Fragments arranged by size: 

                 414     366     353     277
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     692    1410 
   Size:        692     718
 
TatI w'GTAC_w

Cuts at:      0     242    1410 
   Size:        242    1168
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     317     349    1410 
   Size:        317      32    1061

  Fragments arranged by size: 

                1061     317      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     637    1410 
   Size:        637     773
 
XmaI C'CCGG_G

Cuts at:      0    1274    1410 
   Size:       1274     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     190     772    1410 
   Size:        190     582     638

  Fragments arranged by size: 

                 638     582     190
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     AvaI     BaeI    BanII    BbvCI     BcgI
    BciVI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI
   BstEII    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII  HindIII     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI     PfoI     PmlI
    PpuMI     PstI     SacI     SfcI    SgrAI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII    BamHI     BanI     BbeI     BbsI     BclI
    BfrBI    BglII    BmgBI     BmtI     BsaI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI    BspEI    BsrBI    BsrGI   BssHII    BstBI    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     XbaI     XcmI     XhoI     ZraI
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