Restriction map for pF1KB9402
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccIII      AcsI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaI        BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KpnI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RcaI        RsrII       SacI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AclI          643  AACGTT               AA^CGTT
AcyI           36  GGCGTC               G[GA]^CG[CT]C
ApaI          171  GGGCCC               GGGCC^C
AspI         1444  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1582  GGTGCC               G^G[CT][GA]CC
BbvCI         904  CCTCAGC              CCTCAGC(-5/-2)
BglI          824  GCCGGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         141  TACGTG               [CT]AC^GT[GA]
BsaBI          81  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          36  GGCGTC               G[GA]^CG[CT]C
BsaWI         624  TCCGGT               [AT]^CCGG[AT]
BsgI          932  CTGCAC               GTGCAG(16/14)
BsmBI        1442  GAGACG               CGTCTC(1/5)
BsrBI         505  GAGCGG               CCGCTC(-3/-3)
BssSI         634  CACGAG               CACGAG(-5/-1)
BstXI        1160  CCAAGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT14I       176  CCAAGG               C^C[AT][AT]GG
Hin1I          36  GGCGTC               G[GA]^CG[CT]C
HincII        895  GTCGAC               GT[CT]^[GA]AC
HindII        895  GTCGAC               GT[CT]^[GA]AC
KspI          604  CCGCGG               CCGC^GG
MamI           81  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI          419  GCATGC               [GA]CATG^[CT]
PflFI        1444  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         137  CCACTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          183  GACTC                GAGTC(4/5)
PpuMI         397  AGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I      643  AACGTT               AA^CGTT
PvuII         521  CAGCTG               CAG^CTG
SacII         604  CCGCGG               CCGC^GG
SalI          895  GTCGAC               G^TCGAC
SmaI          209  CCCGGG               CCC^GGG
SphI          419  GCATGC               GCATG^C
StuI          901  AGGCCT               AGG^CCT
StyI          176  CCAAGG               C^C[AT][AT]GG
Tsp509I      1014  AATT                 ^AATT
Tth111I      1444  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        137  CCACTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         209  CCCGGG               C^CCGGG
XmaI          209  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          895  GTCGAC               GT^[AC][GT]AC
             1315  GTAGAC              
Alw44I        322  GTGCAC               G^TGCAC
              883  GTGCAC              
AlwNI        1297  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1555  CAGCCACTG           
ApaLI         322  GTGCAC               G^TGCAC
              883  GTGCAC              
AvaI          209  CCCGGG               C^[CT]CG[GA]G
             1408  CCCGAG              
BbsI          687  GAAGAC               GAAGAC(2/6)
             1335  GAAGAC              
BfaI          221  CTAG                 C^TAG
              236  CTAG                
BpuAI         687  GAAGAC               GAAGAC(2/6)
             1335  GAAGAC              
BsoBI         209  CCCGGG               C^[CT]CG[GA]G
             1408  CCCGAG              
BspMI          30  GCAGGT               ACCTGC(4/8)
              318  GCAGGT              
BsrFI         100  ACCGGC               [GA]^CCGG[CT]
              314  ACCGGC              
BstEII         49  GGTGACC              G^GT[ACGT]ACC
             1708  GGTAACC             
BstPI          49  GGTGACC              G^GT[ACGT]ACC
             1708  GGTAACC             
BstYI         746  AGATCC               [GA]^GATC[CT]
             1629  AGATCC              
Cfr10I        100  ACCGGC               [GA]^CCGG[CT]
              314  ACCGGC              
EaeI          554  CGGCCA               [CT]^GGCC[GA]
              652  CGGCCA              
EcoNI         569  CCTCCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1284  CCTGGAGAAGG         
EcoO65I        49  GGTGACC              G^GT[ACGT]ACC
             1708  GGTAACC             
HaeII           4  AGCGCC               [GA]GCGC^[CT]
              304  GGCGCT              
MaeI          221  CTAG                 C^TAG
              236  CTAG                
MaeII         142  ACGT                 A^CGT
              644  ACGT                
MflI          746  AGATCC               [GA]^GATC[CT]
             1629  AGATCC              
PstI         1205  CTGCAG               CTGCA^G
             1561  CTGCAG              
XcmI           23  CCATGATGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1376  CCAAAGAGCTGCTGG     
XhoII         746  AGATCC               [GA]^GATC[CT]
             1629  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        TseI        Tsp45I      TspRI       TthHB8I     
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