Restriction map for pF1KB9402
 (Linear) (Six-base) MAPSORT of: KIBB9402.seq  Check: 99  from: 1  to: 1725



KIBB9402.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      21     309    1725 
   Size:         21     288    1416

  Fragments arranged by size: 

                1416     288      21
 
AccI GT'mk_AC

Cuts at:      0     896    1316    1725 
   Size:        896     420     409

  Fragments arranged by size: 

                 896     420     409
 
AclI AA'CG_TT

Cuts at:      0     644    1725 
   Size:        644    1081
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1302    1560    1725 
   Size:       1302     258     165

  Fragments arranged by size: 

                1302     258     165
 
ApaI G_GGCC'C

Cuts at:      0     175    1725 
   Size:        175    1550
 
ApaLI G'TGCA_C

Cuts at:      0     322     883    1725 
   Size:        322     561     842

  Fragments arranged by size: 

                 842     561     322
 
AvaI C'yCGr_G

Cuts at:      0     209    1408    1725 
   Size:        209    1199     317

  Fragments arranged by size: 

                1199     317     209
 
BanI G'GyrC_C

Cuts at:      0    1582    1725 
   Size:       1582     143
 
BanII G_rGCy'C

Cuts at:      0     175     220     770     835    1725 
   Size:        175      45     550      65     890

  Fragments arranged by size: 

                 890     550     175      65      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     694    1342    1725 
   Size:        694     648     383

  Fragments arranged by size: 

                 694     648     383
 
BbvCI CC'TCA_GC

Cuts at:      0     905    1725 
   Size:        905     820
 
BglI GCCn_nnn'nGGC

Cuts at:      0     830    1725 
   Size:        830     895
 
Bme1580I G_kGCm'C

Cuts at:      0     164     175     326     887    1725 
   Size:        164      11     151     561     838

  Fragments arranged by size: 

                 838     561     164     151      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     396     507     771     973    1725 
   Size:        396     111     264     202     752

  Fragments arranged by size: 

                 752     396     264     202     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1091    1306    1324    1513    1725 
   Size:        503     588     215      18     189     212

  Fragments arranged by size: 

                 588     503     215     212     189      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     503     905    1725 
   Size:        503     402     820

  Fragments arranged by size: 

                 820     503     402
 
BsaAI yAC'GTr

Cuts at:      0     143    1725 
   Size:        143    1582
 
BsaBI GATnn'nnATC

Cuts at:      0      85    1725 
   Size:         85    1640
 
BsaHI Gr'CG_yC

Cuts at:      0      37    1725 
   Size:         37    1688
 
BsaWI w'CCGG_w

Cuts at:      0     624    1725 
   Size:        624    1101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     977    1007    1431    1461    1725 
   Size:        977      30     424      30     264

  Fragments arranged by size: 

                 977     424     264      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     380     540    1112    1725 
   Size:        380     160     572     613

  Fragments arranged by size: 

                 613     572     380     160
 
BseYI C'CCAG_C

Cuts at:      0     260     344     479     486     662     699     944    1126 
   Size:        260      84     135       7     176      37     245     182

Cuts at:   1126    1192    1534    1646    1684    1713    1725 
   Size:         66     342     112      38      29      12

  Fragments arranged by size: 

                 342     260     245     182     176     135     112      84
                  66      38      37      29      12       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     917    1725 
   Size:        917     808
 
BsiHKAI G_wGCw'C

Cuts at:      0     296     326     887    1725 
   Size:        296      30     561     838

  Fragments arranged by size: 

                 838     561     296      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1436    1725 
   Size:       1436     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     175     220     296     326     770     835     887 
   Size:        164      11      45      76      30     444      65      52

Cuts at:    887    1725 
   Size:        838

  Fragments arranged by size: 

                 838     444     164      76      65      52      45      30
                  11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      21     309    1725 
   Size:         21     288    1416

  Fragments arranged by size: 

                1416     288      21
 
BsrBI CCG'CTC

Cuts at:      0     507    1725 
   Size:        507    1218
 
BsrFI r'CCGG_y

Cuts at:      0     100     314    1725 
   Size:        100     214    1411

  Fragments arranged by size: 

                1411     214     100
 
BssSI C'ACGA_G

Cuts at:      0     634    1725 
   Size:        634    1091
 
BstEII G'GTnAC_C

Cuts at:      0      49    1708    1725 
   Size:         49    1659      17

  Fragments arranged by size: 

                1659      49      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1167    1725 
   Size:       1167     558
 
BstYI r'GATC_y

Cuts at:      0     746    1629    1725 
   Size:        746     883      96

  Fragments arranged by size: 

                 883     746      96
 
BtgI C'CryG_G

Cuts at:      0     495     604     890    1586    1725 
   Size:        495     109     286     696     139

  Fragments arranged by size: 

                 696     495     286     139     109
 
BtsI GCAGTG_nn'

Cuts at:      0    1196    1558    1725 
   Size:       1196     362     167

  Fragments arranged by size: 

                1196     362     167
 
DraIII CAC_nnn'GTG

Cuts at:      0     143     252     891    1725 
   Size:        143     109     639     834

  Fragments arranged by size: 

                 834     639     143     109
 
EaeI y'GGCC_r

Cuts at:      0     554     652    1725 
   Size:        554      98    1073

  Fragments arranged by size: 

                1073     554      98
 
EarI CTCTTCn'nnn_

Cuts at:      0    1158    1172    1358    1725 
   Size:       1158      14     186     367

  Fragments arranged by size: 

                1158     367     186      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1091    1306    1324    1513    1725 
   Size:        503     588     215      18     189     212

  Fragments arranged by size: 

                 588     503     215     212     189      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     573    1288    1725 
   Size:        573     715     437

  Fragments arranged by size: 

                 715     573     437
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     172     398    1725 
   Size:        171       1     226    1327

  Fragments arranged by size: 

                1327     226     171       1
 
HaeII r_GCGC'y

Cuts at:      0       8     308    1725 
   Size:          8     300    1417

  Fragments arranged by size: 

                1417     300       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97     172     204     523     555     977    1009 
   Size:         65      32      75      32     319      32     422      32

Cuts at:   1009    1433    1465    1725 
   Size:        424      32     260

  Fragments arranged by size: 

                 424     422     319     260      75      65      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     897    1725 
   Size:        897     828
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     963    1725 
   Size:        291     672     762

  Fragments arranged by size: 

                 762     672     291
 
MslI CAynn'nnrTG

Cuts at:      0     289     625     880    1576    1725 
   Size:        289     336     255     696     149

  Fragments arranged by size: 

                 696     336     289     255     149
 
MspA1I CmG'CkG

Cuts at:      0     385     466     523     606    1725 
   Size:        385      81      57      83    1119

  Fragments arranged by size: 

                1119     385      83      81      57
 
NspI r_CATG'y

Cuts at:      0     423    1725 
   Size:        423    1302
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     143    1725 
   Size:        143    1582
 
PfoI T'CCnGG_A

Cuts at:      0     999    1257    1283    1725 
   Size:        999     258      26     442

  Fragments arranged by size: 

                 999     442     258      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     398    1725 
   Size:        398    1327
 
PspOMI G'GGCC_C

Cuts at:      0     171    1725 
   Size:        171    1554
 
PstI C_TGCA'G

Cuts at:      0    1209    1565    1725 
   Size:       1209     356     160

  Fragments arranged by size: 

                1209     356     160
 
PvuII CAG'CTG

Cuts at:      0     523    1725 
   Size:        523    1202
 
SacII CC_GC'GG

Cuts at:      0     607    1725 
   Size:        607    1118
 
SalI G'TCGA_C

Cuts at:      0     895    1725 
   Size:        895     830
 
SfcI C'TryA_G

Cuts at:      0    1172    1205    1313    1561    1725 
   Size:       1172      33     108     248     164

  Fragments arranged by size: 

                1172     248     164     108      33
 
SmaI CCC'GGG

Cuts at:      0     211    1725 
   Size:        211    1514
 
SphI G_CATG'C

Cuts at:      0     423    1725 
   Size:        423    1302
 
StuI AGG'CCT

Cuts at:      0     903    1725 
   Size:        903     822
 
StyI C'CwwG_G

Cuts at:      0     176    1725 
   Size:        176    1549
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28    1570    1725 
   Size:         28    1542     155

  Fragments arranged by size: 

                1542     155      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1431    1463    1725 
   Size:       1431      32     262

  Fragments arranged by size: 

                1431     262      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1447    1725 
   Size:       1447     278
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30    1383    1725 
   Size:         30    1353     342

  Fragments arranged by size: 

                1353     342      30
 
XmaI C'CCGG_G

Cuts at:      0     209    1725 
   Size:        209    1516
 

 Enzymes that do cut:

     AarI     AccI     AclI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI    BbvCI     BglI Bme1580I     BmrI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstEII
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I
     NspI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII
     SalI     SfcI     SmaI     SphI     StuI     StyI    TaqII     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI     TatI     XbaI     XhoI     XmnI
     ZraI
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