Restriction map for
pF1KB8019
(Circular) (Six-base) MAPSORT of: pF1KB8019.seq Check: 5725 from: 1 to: 5123
pF1KB8019 5123 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1005 1005
Size: 5123
AccI GT'mk_AC
Cuts at: 2161 2161
Size: 5123
Acc65I G'GTAC_C
Cuts at: 2139 2139
Size: 5123
AclI AA'CG_TT
Cuts at: 4963 4963
Size: 5123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 1477 2948 3380 4127 4395 801
Size: 676 1471 432 747 268 1529
Fragments arranged by size:
1529 1471 747 676 432 268
AflIII A'CryG_T
Cuts at: 1004 1401 2475 3579 4556 1004
Size: 397 1074 1104 977 1571
Fragments arranged by size:
1571 1104 1074 977 397
AgeI A'CCGG_T
Cuts at: 3511 3511
Size: 5123
AhdI GACnn_n'nnGTC
Cuts at: 639 2017 639
Size: 1378 3745
AleI CACnn'nnGTG
Cuts at: 1402 1402
Size: 5123
AlwNI CAG_nnn'CTG
Cuts at: 2428 3995 2428
Size: 1567 3556
ApaI G_GGCC'C
Cuts at: 1097 1788 1097
Size: 691 4432
ApaLI G'TGCA_C
Cuts at: 232 3893 232
Size: 3661 1462
ApoI r'AATT_y
Cuts at: 244 2127 4476 4732 244
Size: 1883 2349 256 635
Fragments arranged by size:
2349 1883 635 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
AvaI C'yCGr_G
Cuts at: 181 1311 1659 1698 1788 1947 2005 2143 181
Size: 1130 348 39 90 159 58 138 3161
Fragments arranged by size:
3161 1130 348 159 138 90 58 39
BamHI G'GATC_C
Cuts at: 2148 2148
Size: 5123
BanI G'GyrC_C
Cuts at: 590 1251 1854 2139 2553 2801 2836 590
Size: 661 603 285 414 248 35 2877
Fragments arranged by size:
2877 661 603 414 285 248 35
BanII G_rGCy'C
Cuts at: 998 1097 1227 1788 1955 2137 3167 998
Size: 99 130 561 167 182 1030 2954
Fragments arranged by size:
2954 1030 561 182 167 130 99
BbeI G_GCGC'C
Cuts at: 1255 2557 2805 1255
Size: 1302 248 3573
Fragments arranged by size:
3573 1302 248
BbsI GAAGACnn'nnnn_
Cuts at: 1512 1512
Size: 5123
BbvCI CC'TCA_GC
Cuts at: 359 652 359
Size: 293 4830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 772 806 885 919 1524 1558 772
Size: 34 79 34 605 34 4337
Fragments arranged by size:
4337 605 79 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3021 3782 5123 3021
Size: 761 1341 3021
Fragments arranged by size:
3021 1341 761
BclI T'GATC_A
Cuts at: 2643 2643
Size: 5123
BglII A'GATC_T
Cuts at: 293 2638 4675 293
Size: 2345 2037 741
Fragments arranged by size:
2345 2037 741
BlpI GC'TnA_GC
Cuts at: 217 715 1903 2235 217
Size: 498 1188 332 3105
Fragments arranged by size:
3105 1188 498 332
Bme1580I G_kGCm'C
Cuts at: 236 394 552 593 1097 1788 2748 2841 3897
Size: 158 158 41 504 691 960 93 1056
Cuts at: 3897 236
Size: 1462
Fragments arranged by size:
1462 1056 960 691 504 158 158 93
41
BmgBI CAC'GTC
Cuts at: 1026 1380 1026
Size: 354 4769
BmrI ACTGGGnnnn_n'
Cuts at: 2335 2416 2751 4895 2335
Size: 81 335 2144 2563
Fragments arranged by size:
2563 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3490 3490
Size: 5123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 1554 1339
Size: 215 4908
Bpu10I CC'TnA_GC
Cuts at: 359 652 359
Size: 293 4830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 685 1327 2299 3178 3670 3968 445
Size: 240 642 972 879 492 298 1600
Fragments arranged by size:
1600 972 879 642 492 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 109 345 596 4827 109
Size: 236 251 4231 405
Fragments arranged by size:
4231 405 251 236
BsaAI yAC'GTr
Cuts at: 1404 2476 1404
Size: 1072 4051
BsaBI GATnn'nnATC
Cuts at: 6 1351 2637 4674 6
Size: 1345 1286 2037 455
Fragments arranged by size:
2037 1345 1286 455
BsaHI Gr'CG_yC
Cuts at: 151 555 1252 2554 2802 3504 5000 151
Size: 404 697 1302 248 702 1496 274
Fragments arranged by size:
1496 1302 702 697 404 274 248
BsaWI w'CCGG_w
Cuts at: 755 2312 2833 3511 3785 3932 4343 755
Size: 1557 521 678 274 147 411 1535
Fragments arranged by size:
1557 1535 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 793 823 934 964 1552 1582 139
Size: 30 624 30 111 30 588 30 3680
Fragments arranged by size:
3680 624 588 111 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 160 304 583 801 157
Size: 3 144 279 218 4479
Fragments arranged by size:
4479 279 218 144 3
BseYI C'CCAG_C
Cuts at: 1121 1822 2548 3306 3883 4379 1121
Size: 701 726 758 577 496 1865
Fragments arranged by size:
1865 758 726 701 577 496
BsiEI CG_ry'CG
Cuts at: 104 333 924 987 1332 1895 2665 2711 3919
Size: 229 591 63 345 563 770 46 1208
Cuts at: 3919 104
Size: 1308
Fragments arranged by size:
1308 1208 770 591 563 345 229 63
46
BsiHKAI G_wGCw'C
Cuts at: 218 236 369 739 2137 2915 3105 3897 218
Size: 18 133 370 1398 778 190 792 1444
Fragments arranged by size:
1444 1398 792 778 370 190 133 18
BsmBI CGTCTCn'nnnn_
Cuts at: 142 923 142
Size: 781 4342
Bsp1286I G_dGCh'C
Cuts at: 218 236 369 394 552 593 739 998 1097
Size: 18 133 25 158 41 146 259 99
Cuts at: 1097 1227 1788 1955 2137 2748 2841 2915 3105
Size: 130 561 167 182 611 93 74 190
Cuts at: 3105 3167 3897 218
Size: 62 730 1444
Fragments arranged by size:
1444 730 611 561 259 190 182 167
158 146 133 130 99 93 74 62
41 25 18
BspEI T'CCGG_A
Cuts at: 755 4343 755
Size: 3588 1535
BspHI T'CATG_A
Cuts at: 956 4299 956
Size: 3343 1780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 1005 2173 2387 2689 3070 419
Size: 586 1168 214 302 381 2472
Fragments arranged by size:
2472 1168 586 381 302 214
BsrBI CCG'CTC
Cuts at: 50 429 1422 3415 3469 4953 5118 50
Size: 379 993 1993 54 1484 165 55
Fragments arranged by size:
1993 1484 993 379 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1053 3035 1053
Size: 1982 3141
BsrFI r'CCGG_y
Cuts at: 224 536 770 1894 3121 3302 3511 224
Size: 312 234 1124 1227 181 209 1836
Fragments arranged by size:
1836 1227 1124 312 234 209 181
BsrGI T'GTAC_A
Cuts at: 1394 1394
Size: 5123
BssSI C'ACGA_G
Cuts at: 705 3394 3752 705
Size: 2689 358 2076
Fragments arranged by size:
2689 2076 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2500 2500
Size: 5123
BstBI TT'CG_AA
Cuts at: 3484 3484
Size: 5123
BstYI r'GATC_y
Cuts at: 1 293 1322 1527 2148 2615 2638 2973 3219
Size: 292 1029 205 621 467 23 335 246
Cuts at: 3219 4231 4317 4329 4675 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1029 1012 621 467 449 346 335 292
246 205 86 23 12
BtgI C'CryG_G
Cuts at: 107 173 260 524 725 924 1156 1908 107
Size: 66 87 264 201 199 232 752 3322
Fragments arranged by size:
3322 752 264 232 201 199 87 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1338 3073 3254 3417 1338
Size: 1735 181 163 3044
Fragments arranged by size:
3044 1735 181 163
BtsI GCAGTG_nn'
Cuts at: 1513 1764 1836 1842 2366 1513
Size: 251 72 6 524 4270
Fragments arranged by size:
4270 524 251 72 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 906 941 1740 1775 906
Size: 35 799 35 4254
Fragments arranged by size:
4254 799 35 35
DraI TTT'AAA
Cuts at: 2122 2122
Size: 5123
DraIII CAC_nnn'GTG
Cuts at: 1861 1861
Size: 5123
DrdI GACnn_nn'nnGTC
Cuts at: 2829 3687 2829
Size: 858 4265
EaeI y'GGCC_r
Cuts at: 197 330 479 974 984 1112 1892 1896 2708
Size: 133 149 495 10 128 780 4 812
Cuts at: 2708 2882 3273 3300 4645 197
Size: 174 391 27 1345 675
Fragments arranged by size:
1345 812 780 675 495 391 174 149
133 128 27 10 4
EagI C'GGCC_G
Cuts at: 330 984 1892 2708 330
Size: 654 908 816 2745
Fragments arranged by size:
2745 908 816 654
EarI CTCTTCn'nnn_
Cuts at: 1241 1261 3146 3356 1241
Size: 20 1885 210 3008
Fragments arranged by size:
3008 1885 210 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 789 814 1390 3331 3641 3787 4933 127
Size: 662 25 576 1941 310 146 1146 317
Fragments arranged by size:
1941 1146 662 576 317 310 146 25
EcoICRI GAG'CTC
Cuts at: 2135 2135
Size: 5123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 1339 1477 1554 2948 3380 4127 4395 801
Size: 538 138 77 1394 432 747 268 1529
Fragments arranged by size:
1529 1394 747 538 432 268 138 77
EcoNI CCTnn'n_nnAGG
Cuts at: 358 793 358
Size: 435 4688
EcoO109I rG'GnC_Cy
Cuts at: 383 1093 1125 1663 1695 1785 2011 2262 383
Size: 710 32 538 32 90 226 251 3244
Fragments arranged by size:
3244 710 538 251 226 90 32 32
EcoRI G'AATT_C
Cuts at: 2127 2127
Size: 5123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1482 1512 3142 1482
Size: 30 1630 3463
Fragments arranged by size:
3463 1630 30
FseI GG_CCGG'CC
Cuts at: 1898 1898
Size: 5123
FspI TGC'GCA
Cuts at: 2904 2904
Size: 5123
HaeII r_GCGC'y
Cuts at: 789 1255 1283 2557 2805 3827 4806 789
Size: 466 28 1274 248 1022 979 1106
Fragments arranged by size:
1274 1106 1022 979 466 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 625 652 657 684 793 825 1307
Size: 32 226 27 5 27 109 32 482
Cuts at: 1307 1339 1569 1601 2003 2035 2965 2995 2997
Size: 32 230 32 402 32 930 30 2
Cuts at: 2997 3027 367
Size: 30 2463
Fragments arranged by size:
2463 930 482 402 230 226 109 32
32 32 32 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 1485 2162 1485
Size: 677 4446
HindIII A'AGCT_T
Cuts at: 313 313
Size: 5123
KasI G'GCGC_C
Cuts at: 1251 2553 2801 1251
Size: 1302 248 3573
Fragments arranged by size:
3573 1302 248
KpnI G_GTAC'C
Cuts at: 2143 2143
Size: 5123
MluI A'CGCG_T
Cuts at: 4556 4556
Size: 5123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3794 3978 3794
Size: 184 4939
MscI TGG'CCA
Cuts at: 481 1114 2884 481
Size: 633 1770 2720
Fragments arranged by size:
2720 1770 633
MslI CAynn'nnrTG
Cuts at: 374 473 1402 3112 3239 374
Size: 99 929 1710 127 2258
Fragments arranged by size:
2258 1710 929 127 99
MspA1I CmG'CkG
Cuts at: 979 1257 1302 1605 1718 1877 2234 2548 2908
Size: 278 45 303 113 159 357 314 360
Cuts at: 2908 3921 4166 979
Size: 1013 245 1936
Fragments arranged by size:
1936 1013 360 357 314 303 278 245
159 113 45
NaeI GCC'GGC
Cuts at: 772 1896 3304 772
Size: 1124 1408 2591
Fragments arranged by size:
2591 1408 1124
NarI GG'CG_CC
Cuts at: 1252 2554 2802 1252
Size: 1302 248 3573
Fragments arranged by size:
3573 1302 248
NcoI C'CATG_G
Cuts at: 107 260 524 107
Size: 153 264 4706
Fragments arranged by size:
4706 264 153
NgoMIV G'CCGG_C
Cuts at: 770 1894 3302 770
Size: 1124 1408 2591
Fragments arranged by size:
2591 1408 1124
NspI r_CATG'y
Cuts at: 1008 1338 2176 2676 3583 1008
Size: 330 838 500 907 2548
Fragments arranged by size:
2548 907 838 500 330
PasI CC'CwG_GG
Cuts at: 387 387
Size: 5123
PciI A'CATG_T
Cuts at: 1004 3579 1004
Size: 2575 2548
PflMI CCAn_nnn'nTGG
Cuts at: 1121 1121
Size: 5123
PfoI T'CCnGG_A
Cuts at: 2021 2021
Size: 5123
PmeI GTTT'AAAC
Cuts at: 2122 2122
Size: 5123
PmlI CAC'GTG
Cuts at: 1404 1404
Size: 5123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 4262
Size: 32 5091
PpuMI rG'GwC_Cy
Cuts at: 383 1125 1663 1695 2011 383
Size: 742 538 32 316 3495
Fragments arranged by size:
3495 742 538 316 32
PspOMI G'GGCC_C
Cuts at: 1093 1784 1093
Size: 691 4432
PstI C_TGCA'G
Cuts at: 1016 1537 2170 2855 1016
Size: 521 633 685 3284
Fragments arranged by size:
3284 685 633 521
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5123
PvuII CAG'CTG
Cuts at: 1302 1877 2548 2908 1302
Size: 575 671 360 3517
Fragments arranged by size:
3517 671 575 360
RsrII CG'GwC_CG
Cuts at: 801 3318 801
Size: 2517 2606
SacI G_AGCT'C
Cuts at: 2137 2137
Size: 5123
SalI G'TCGA_C
Cuts at: 2160 2160
Size: 5123
SanDI GG'GwC_CC
Cuts at: 383 1125 1663 2011 383
Size: 742 538 348 3495
Fragments arranged by size:
3495 742 538 348
SapI GCTCTTCn'nnn_
Cuts at: 3146 3356 3146
Size: 210 4913
SbfI CC_TGCA'GG
Cuts at: 2170 2170
Size: 5123
ScaI AGT'ACT
Cuts at: 4640 4640
Size: 5123
SexAI A'CCwGG_T
Cuts at: 158 158
Size: 5123
SfcI C'TryA_G
Cuts at: 33 1012 1533 2166 2851 3844 4035 33
Size: 979 521 633 685 993 191 1121
Fragments arranged by size:
1121 993 979 685 633 521 191
SfoI GGC'GCC
Cuts at: 1253 2555 2803 1253
Size: 1302 248 3573
Fragments arranged by size:
3573 1302 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
SgrAI Cr'CCGG_yG
Cuts at: 536 770 536
Size: 234 4889
SmaI CCC'GGG
Cuts at: 1661 1700 1790 1949 2007 2145 1661
Size: 39 90 159 58 138 4639
Fragments arranged by size:
4639 159 138 90 58 39
SmlI C'TyrA_G
Cuts at: 424 664 1342 2278 3193 3685 3947 424
Size: 240 678 936 915 492 262 1600
Fragments arranged by size:
1600 936 915 678 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5123
SphI G_CATG'C
Cuts at: 2176 2676 2176
Size: 500 4623
StuI AGG'CCT
Cuts at: 1933 1981 1933
Size: 48 5075
StyI C'CwwG_G
Cuts at: 107 260 524 595 1976 2257 2611 107
Size: 153 264 71 1381 281 354 2619
Fragments arranged by size:
2619 1381 354 281 264 153 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 608 620 2706 2839 3298 3507 608
Size: 12 2086 133 459 209 2224
Fragments arranged by size:
2224 2086 459 209 133 12
TatI w'GTAC_w
Cuts at: 1394 4638 1394
Size: 3244 1879
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1661 1693 4640 4672 1661
Size: 32 2947 32 2112
Fragments arranged by size:
2947 2112 32 32
Tth111I GACn'n_nGTC
Cuts at: 2920 2920
Size: 5123
XbaI T'CTAG_A
Cuts at: 2154 2154
Size: 5123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 1906 2618 678
Size: 1228 712 3183
Fragments arranged by size:
3183 1228 712
XmaI C'CCGG_G
Cuts at: 1659 1698 1788 1947 2005 2143 1659
Size: 39 90 159 58 138 4639
Fragments arranged by size:
4639 159 138 90 58 39
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BglI BmtI BsgI BsiWI BsmI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SfiI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI