Restriction map for
pF1KB8009
(Circular) (Six-base) MAPSORT of: pF1KB8009.seq Check: 1344 from: 1 to: 4262
pF1KB8009 4262 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 187 187
Size: 4262
AccI GT'mk_AC
Cuts at: 1300 1300
Size: 4262
Acc65I G'GTAC_C
Cuts at: 987 1278 987
Size: 291 3971
AclI AA'CG_TT
Cuts at: 4102 4102
Size: 4262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 716 1015 1093 2087 2519 3266 3534 623
Size: 93 299 78 994 432 747 268 1351
Fragments arranged by size:
1351 994 747 432 299 268 93 78
AflIII A'CryG_T
Cuts at: 1614 2718 3695 1614
Size: 1104 977 2181
Fragments arranged by size:
2181 1104 977
AgeI A'CCGG_T
Cuts at: 2650 2650
Size: 4262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 920 1247 920
Size: 327 3935
AlwNI CAG_nnn'CTG
Cuts at: 1567 3134 1567
Size: 1567 2695
ApaLI G'TGCA_C
Cuts at: 560 3032 560
Size: 2472 1790
ApoI r'AATT_y
Cuts at: 675 847 1016 1251 1266 3615 3871 675
Size: 172 169 235 15 2349 256 1066
Fragments arranged by size:
2349 1066 256 235 172 169 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4262
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4262
AvaI C'yCGr_G
Cuts at: 1282 1282
Size: 4262
BamHI G'GATC_C
Cuts at: 1287 1287
Size: 4262
BanI G'GyrC_C
Cuts at: 987 1278 1692 1940 1975 987
Size: 291 414 248 35 3274
Fragments arranged by size:
3274 414 291 248 35
BanII G_rGCy'C
Cuts at: 1050 1276 2306 1050
Size: 226 1030 3006
Fragments arranged by size:
3006 1030 226
BbeI G_GCGC'C
Cuts at: 1696 1944 1696
Size: 248 4014
BbsI GAAGACnn'nnnn_
Cuts at: 253 575 253
Size: 322 3940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4228
BciVI GTATCCnnnnn_n'
Cuts at: 1098 2160 2921 4262 1098
Size: 1062 761 1341 1098
Fragments arranged by size:
1341 1098 1062 761
BclI T'GATC_A
Cuts at: 927 1782 927
Size: 855 3407
BglII A'GATC_T
Cuts at: 1777 3814 1777
Size: 2037 2225
BlpI GC'TnA_GC
Cuts at: 1374 1374
Size: 4262
Bme1580I G_kGCm'C
Cuts at: 564 1887 1980 3036 564
Size: 1323 93 1056 1790
Fragments arranged by size:
1790 1323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1474 1555 1890 4034 1474
Size: 81 335 2144 1702
Fragments arranged by size:
2144 1702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 812 2629 812
Size: 1817 2445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 807 317
Size: 490 3772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1438 2317 2809 3107 820
Size: 618 879 492 298 1975
Fragments arranged by size:
1975 879 618 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3966 3966
Size: 4262
BsaAI yAC'GTr
Cuts at: 171 1615 171
Size: 1444 2818
BsaBI GATnn'nnATC
Cuts at: 6 1776 3813 6
Size: 1770 2037 455
Fragments arranged by size:
2037 1770 455
BsaHI Gr'CG_yC
Cuts at: 184 1693 1941 2643 4139 184
Size: 1509 248 702 1496 307
Fragments arranged by size:
1509 1496 702 307 248
BsaWI w'CCGG_w
Cuts at: 1451 1972 2650 2924 3071 3482 1451
Size: 521 678 274 147 411 2231
Fragments arranged by size:
2231 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 290 320 290
Size: 30 4232
BseYI C'CCAG_C
Cuts at: 1687 2445 3022 3518 1687
Size: 758 577 496 2431
Fragments arranged by size:
2431 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 374 178
Size: 196 4066
BsiEI CG_ry'CG
Cuts at: 104 152 1804 1850 3058 104
Size: 48 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 564 909 1276 2054 2244 3036 564
Size: 345 367 778 190 792 1790
Fragments arranged by size:
1790 792 778 367 345 190
BsmI GAATG_Cn'
Cuts at: 617 1124 617
Size: 507 3755
BsmBI CGTCTCn'nnnn_
Cuts at: 175 175
Size: 4262
Bsp1286I G_dGCh'C
Cuts at: 564 909 1050 1276 1887 1980 2054 2244 2306
Size: 345 141 226 611 93 74 190 62
Cuts at: 2306 3036 564
Size: 730 1790
Fragments arranged by size:
1790 730 611 345 226 190 141 93
74 62
BspEI T'CCGG_A
Cuts at: 3482 3482
Size: 4262
BspHI T'CATG_A
Cuts at: 354 1136 3438 354
Size: 782 2302 1178
Fragments arranged by size:
2302 1178 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 999 1312 1526 1828 2209 999
Size: 313 214 302 381 3052
Fragments arranged by size:
3052 381 313 302 214
BsrBI CCG'CTC
Cuts at: 50 2554 2608 4092 4257 50
Size: 2504 54 1484 165 55
Fragments arranged by size:
2504 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2174 2174
Size: 4262
BsrFI r'CCGG_y
Cuts at: 147 2260 2441 2650 147
Size: 2113 181 209 1759
Fragments arranged by size:
2113 1759 209 181
BssSI C'ACGA_G
Cuts at: 1043 2533 2891 1043
Size: 1490 358 2414
Fragments arranged by size:
2414 1490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1639 1639
Size: 4262
BstBI TT'CG_AA
Cuts at: 2623 2623
Size: 4262
BstXI CCAn_nnnn'nTGG
Cuts at: 1243 1243
Size: 4262
BstYI r'GATC_y
Cuts at: 1 1287 1754 1777 2112 2358 3370 3456 3468
Size: 1286 467 23 335 246 1012 86 12
Cuts at: 3468 3814 1
Size: 346 449
Fragments arranged by size:
1286 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 687 687
Size: 4262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2212 2393 2556 2212
Size: 181 163 3918
Fragments arranged by size:
3918 181 163
BtsI GCAGTG_nn'
Cuts at: 1231 1505 1231
Size: 274 3988
DraI TTT'AAA
Cuts at: 1261 1261
Size: 4262
DrdI GACnn_nn'nnGTC
Cuts at: 1968 2826 1968
Size: 858 3404
EaeI y'GGCC_r
Cuts at: 149 544 1847 2021 2412 2439 3784 149
Size: 395 1303 174 391 27 1345 627
Fragments arranged by size:
1345 1303 627 395 391 174 27
EagI C'GGCC_G
Cuts at: 149 1847 149
Size: 1698 2564
EarI CTCTTCn'nnn_
Cuts at: 363 607 1090 1138 2285 2495 363
Size: 244 483 48 1147 210 2130
Fragments arranged by size:
2130 1147 483 244 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2470 2780 2926 4072 2470
Size: 310 146 1146 2660
Fragments arranged by size:
2660 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1274 1274
Size: 4262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 623 716 807 1015 1093 2087 2519 3266
Size: 306 93 91 208 78 994 432 747
Cuts at: 3266 3534 317
Size: 268 1045
Fragments arranged by size:
1045 994 747 432 306 268 208 93
91 78
EcoO109I rG'GnC_Cy
Cuts at: 1401 1401
Size: 4262
EcoRI G'AATT_C
Cuts at: 1266 1266
Size: 4262
EcoRV GAT'ATC
Cuts at: 255 255
Size: 4262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1149 1207 2281 1149
Size: 58 1074 3130
Fragments arranged by size:
3130 1074 58
FspI TGC'GCA
Cuts at: 2043 2043
Size: 4262
HaeII r_GCGC'y
Cuts at: 1696 1944 2966 3945 1696
Size: 248 1022 979 2013
Fragments arranged by size:
2013 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 174 206 2104 2134 2136 2166 174
Size: 32 1898 30 2 30 2270
Fragments arranged by size:
2270 1898 32 30 30 2
HincII GTy'rAC
Cuts at: 1301 1301
Size: 4262
KasI G'GCGC_C
Cuts at: 1692 1940 1692
Size: 248 4014
KpnI G_GTAC'C
Cuts at: 991 1282 991
Size: 291 3971
MluI A'CGCG_T
Cuts at: 3695 3695
Size: 4262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 256 1181 2933 3117 256
Size: 925 1752 184 1401
Fragments arranged by size:
1752 1401 925 184
MscI TGG'CCA
Cuts at: 2023 2023
Size: 4262
MslI CAynn'nnrTG
Cuts at: 902 2251 2378 902
Size: 1349 127 2786
Fragments arranged by size:
2786 1349 127
MspA1I CmG'CkG
Cuts at: 1092 1373 1687 2047 3060 3305 1092
Size: 281 314 360 1013 245 2049
Fragments arranged by size:
2049 1013 360 314 281 245
NaeI GCC'GGC
Cuts at: 149 2443 149
Size: 2294 1968
NarI GG'CG_CC
Cuts at: 1693 1941 1693
Size: 248 4014
NcoI C'CATG_G
Cuts at: 687 687
Size: 4262
NgoMIV G'CCGG_C
Cuts at: 147 2441 147
Size: 2294 1968
NspI r_CATG'y
Cuts at: 637 1315 1815 2722 637
Size: 678 500 907 2177
Fragments arranged by size:
2177 907 678 500
PciI A'CATG_T
Cuts at: 2718 2718
Size: 4262
PfoI T'CCnGG_A
Cuts at: 819 819
Size: 4262
PmeI GTTT'AAAC
Cuts at: 1261 1261
Size: 4262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 4230
PstI C_TGCA'G
Cuts at: 1014 1309 1994 1014
Size: 295 685 3282
Fragments arranged by size:
3282 685 295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4262
PvuII CAG'CTG
Cuts at: 1092 1687 2047 1092
Size: 595 360 3307
Fragments arranged by size:
3307 595 360
RsrII CG'GwC_CG
Cuts at: 2457 2457
Size: 4262
SacI G_AGCT'C
Cuts at: 1276 1276
Size: 4262
SalI G'TCGA_C
Cuts at: 1299 1299
Size: 4262
SapI GCTCTTCn'nnn_
Cuts at: 1138 2285 2495 1138
Size: 1147 210 2905
Fragments arranged by size:
2905 1147 210
SbfI CC_TGCA'GG
Cuts at: 1309 1309
Size: 4262
ScaI AGT'ACT
Cuts at: 3779 3779
Size: 4262
SfcI C'TryA_G
Cuts at: 33 573 1010 1305 1990 2983 3174 33
Size: 540 437 295 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 437 295 191
SfoI GGC'GCC
Cuts at: 1694 1942 1694
Size: 248 4014
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4262
SmaI CCC'GGG
Cuts at: 1284 1284
Size: 4262
SmlI C'TyrA_G
Cuts at: 799 1417 2332 2824 3086 799
Size: 618 915 492 262 1975
Fragments arranged by size:
1975 915 618 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4262
SphI G_CATG'C
Cuts at: 637 1315 1815 637
Size: 678 500 3084
Fragments arranged by size:
3084 678 500
SspI AAT'ATT
Cuts at: 754 754
Size: 4262
StyI C'CwwG_G
Cuts at: 687 1396 1750 687
Size: 709 354 3199
Fragments arranged by size:
3199 709 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1845 1978 2437 2646 1845
Size: 133 459 209 3461
Fragments arranged by size:
3461 459 209 133
TatI w'GTAC_w
Cuts at: 3777 3777
Size: 4262
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4230
Tth111I GACn'n_nGTC
Cuts at: 2059 2059
Size: 4262
XbaI T'CTAG_A
Cuts at: 1293 1293
Size: 4262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1757 1757
Size: 4262
XmaI C'CCGG_G
Cuts at: 1282 1282
Size: 4262
XmnI GAAnn'nnTTC
Cuts at: 812 812
Size: 4262
ZraI GAC'GTC
Cuts at: 185 185
Size: 4262
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI