Restriction map for pF1KB7014
##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp718I     AvrII       
BciVI       BfrI        BlnI        BseAI       BspEI       
Bst1107I    BstZ17I     CpoI        DraI        EcoRV       
EcoT22I     HindIII     HpaI        KpnI        MfeI        
MluI        MroI        MunI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SalI        SfiI        SnaBI       SpeI        
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          820  GTCTAC               GT^[AC][GT]AC
AhdI          754  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2109  GTGCAC               G^TGCAC
ApaLI        2109  GTGCAC               G^TGCAC
Asp700I      4146  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         754  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1895  GGATCC               G^GATCC
BbrPI        2104  CACGTG               CAC^GTG
BsaBI        2988  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI         935  CGTACG               C^GTACG
BspHI        4232  TCATGA               T^CATGA
BssHII       4744  GCGCGC               G^CGCGC
BstBI        2731  TTCGAA               TT^CGAA
Eam1105I      754  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2358  GAATTC               G^AATTC
MamI         2988  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1160  TTAA                 T^TAA
NcoI         3356  CCATGG               C^CATGG
NdeI         4031  CATATG               CA^TATG
NspV         2731  TTCGAA               TT^CGAA
PmaCI        2104  CACGTG               CAC^GTG
PmlI         2104  CACGTG               CAC^GTG
PshAI        2737  GACGTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         4232  TCATGA               T^CATGA
SfuI         2731  TTCGAA               TT^CGAA
SgrAI         870  CGCCGGCG             C[GA]^CCGG[CT]G
SphI         4928  GCATGC               GCATG^C
StuI         1808  AGGCCT               AGG^CCT
Tru9I        1160  TTAA                 T^TAA
XmnI         4146  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AatII        4768  GACGTC               GACGT^C
             4866  GACGTC              
AcsI          925  AAATTC               [GA]^AATT[CT]
             2358  GAATTC              
ApoI          925  AAATTC               [GA]^AATT[CT]
             2358  GAATTC              
AviII        3392  TGCGCA               TGC^GCA
             4934  TGCGCA              
BbsI         2734  GAAGAC               GAAGAC(2/6)
             2023  GTCTTC              
BclI         4979  TGATCA               T^GATCA
             5312  TGATCA              
BfaI          214  CTAG                 C^TAG
              841  CTAG                
BlpI         5177  GCTCAGC              GC^T[ACGT]AGC
             5560  GCTGAGC             
Bpu1102I     5177  GCTCAGC              GC^T[ACGT]AGC
             5560  GCTGAGC             
BpuAI        2734  GAAGAC               GAAGAC(2/6)
             2023  GTCTTC              
BsaAI        2104  CACGTG               [CT]AC^GT[GA]
             2200  TACGTG              
BsaWI        3086  TCCGGT               [AT]^CCGG[AT]
             4025  ACCGGA              
BseRI        4890  CTCCTC               GAGGAG(10/8)
              682  GAGGAG              
BsmBI        2668  GAGACG               CGTCTC(1/5)
             3661  GAGACG              
BsmI         3937  GAATGC               GAATGC(1/-1)
             4593  GAATGC              
Bsp1407I      794  TGTACA               T^GTACA
             3341  TGTACA              
BspDI         625  ATCGAT               AT^CGAT
             1258  ATCGAT              
BspLU11I      695  ACATGT               A^CATGT
             5204  ACATGT              
BsrDI         573  CATTGC               GCAATG(2/0)
             1766  GCAATG              
BsrGI         794  TGTACA               T^GTACA
             3341  TGTACA              
BstEII       4635  GGTCACC              G^GT[ACGT]ACC
             5056  GGTGACC             
BstPI        4635  GGTCACC              G^GT[ACGT]ACC
             5056  GGTGACC             
BstXI        2225  CCATCACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3986  CCAATGACCTGG        
CelII        5177  GCTCAGC              GC^T[ACGT]AGC
             5560  GCTGAGC             
ClaI          625  ATCGAT               AT^CGAT
             1258  ATCGAT              
DrdI         4762  GACGAGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4830  GACAGACGGGTC        
EagI         1014  CGGCCG               C^GGCCG
             3418  CGGCCG              
EclXI        1014  CGGCCG               C^GGCCG
             3418  CGGCCG              
Eco52I       1014  CGGCCG               C^GGCCG
             3418  CGGCCG              
EcoO65I      4635  GGTCACC              G^GT[ACGT]ACC
             5056  GGTGACC             
FbaI         4979  TGATCA               T^GATCA
             5312  TGATCA              
FseI         1459  GGCCGGCC             GGCCGG^CC
             3046  GGCCGGCC            
FspI         3392  TGCGCA               TGC^GCA
             4934  TGCGCA              
MaeI          214  CTAG                 C^TAG
              841  CTAG                
SapI         5274  GAAGAGC              GCTCTTC(1/4)
              606  GCTCTTC             
ScaI          382  AGTACT               AGT^ACT
              641  AGTACT              
SexAI        4823  ACCAGGT              A^CC[AT]GGT
             5018  ACCTGGT             
SspBI         794  TGTACA               T^GTACA
             3341  TGTACA              
Tsp509I       926  AATT                 ^AATT
             2359  AATT                
XcmI          602  CCAAGCTCTTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3524  CCACTGGCCTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbvCI       
BbvI        BcgI        BcnI        BglI        BglII       
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      SbfI        
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        Sse8387I    StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XhoII       XmaCI       XmaI        
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