Restriction map for pF1KB7007
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AocI        AscI        AseI        AsnI        AvrII       
BcgI        BciVI       BclI        BfrI        BlnI        
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        DraIII      EagI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoRV       
FbaI        FseI        KspI        MroI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
SacII       SapI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SspBI       SwaI        XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        950  GGTACC               G^GTACC
AccII        3197  CGCG                 CG^CG
Alw44I        305  GTGCAC               G^TGCAC
Aor51HI      2622  AGCGCT               AGC^GCT
ApaLI         305  GTGCAC               G^TGCAC
Asp718I       950  GGTACC               G^GTACC
AviII         974  TGCGCA               TGC^GCA
BamHI        1332  GGATCC               G^GATCC
BbeI          715  GGCGCC               GGCGC^C
BbrPI        1266  CACGTG               CAC^GTG
BglII        3104  AGATCT               A^GATCT
BlpI         1188  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1188  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1266  CACGTG               [CT]AC^GT[GA]
BsaBI        1519  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2514  GAGACC               GGTCTC(1/5)
BsaWI        2295  ACCGGA               [AT]^CCGG[AT]
BsmI         2361  GAATGC               GAATGC(1/-1)
BsrFI         277  GCCGGC               [GA]^CCGG[CT]
BstUI        3197  CGCG                 CG^CG
BstXI        2600  CCACGGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1188  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        277  GCCGGC               [GA]^CCGG[CT]
CpoI          380  CGGACCG              CG^G[AT]CCG
DraI         1087  TTTAAA               TTT^AAA
Eco47III     2622  AGCGCT               AGC^GCT
EcoRI        2983  GAATTC               G^AATTC
EcoT22I      2111  ATGCAT               ATGCA^T
FspI          974  TGCGCA               TGC^GCA
KasI          715  GGCGCC               G^GCGCC
KpnI          950  GGTACC               GGTAC^C
MamI         1519  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4094  CAATTG               C^AATTG
MluI         3196  ACGCGT               A^CGCGT
MunI         4094  CAATTG               C^AATTG
MvnI         3197  CGCG                 CG^CG
NaeI          277  GCCGGC               GCC^GGC
NarI          715  GGCGCC               GG^CGCC
NdeI         3565  CATATG               CA^TATG
NgoMIV        277  GCCGGC               G^CCGGC
NsiI         2111  ATGCAT               ATGCA^T
PaeR7I        581  CTCGAG               C^TCGAG
PmaCI        1266  CACGTG               CAC^GTG
PmlI         1266  CACGTG               CAC^GTG
PpuMI         542  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1243  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         380  CGGACCG              CG^G[AT]CCG
SacI          517  GAGCTC               GAGCT^C
SbfI          660  CCTGCAGG             CCTGCA^GG
ScaI         2960  AGTACT               AGT^ACT
SfiI          658  GGCCTGCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          715  GGCGCC               GGC^GCC
Sse8387I      660  CCTGCAGG             CCTGCA^GG
StuI          344  AGGCCT               AGG^CCT
XhoI          581  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          715  GGCGCC               G[GA]^CG[CT]C
             1018  GACGCC              
AhdI         3862  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3949  GACGAAAAGTC         
AspEI        3862  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3949  GACGAAAAGTC         
BbsI         1072  GAAGAC               GAAGAC(2/6)
               41  GTCTTC              
BglI          659  GCCTGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2597  GCCCCACGGGC         
BpuAI        1072  GAAGAC               GAAGAC(2/6)
               41  GTCTTC              
BsaHI         715  GGCGCC               G[GA]^CG[CT]C
             1018  GACGCC              
BspLU11I     1172  ACATGT               A^CATGT
             1971  ACATGT              
BsrDI        1876  GCAATG               GCAATG(2/0)
             2321  GCAATG              
BssSI         336  CTCGTG               CACGAG(-5/-1)
              520  CTCGTG              
BstAPI       2683  GCAAATCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2957  GCAAGTACTGC         
BstYI        1332  GGATCC               [GA]^GATC[CT]
             3104  AGATCT              
DrdI         1018  GACGCCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1282  GACCCCCCCGTC        
Eam1105I     3862  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3949  GACGAAAAGTC         
Hin1I         715  GGCGCC               G[GA]^CG[CT]C
             1018  GACGCC              
HindIII      1853  AAGCTT               A^AGCTT
             3049  AAGCTT              
HpaI         1065  GTTAAC               GTT^AAC
             1116  GTTAAC              
MflI         1332  GGATCC               [GA]^GATC[CT]
             3104  AGATCT              
NcoI          119  CCATGG               C^CATGG
             1965  CCATGG              
PflMI         483  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              561  CCAGCGTCTGG         
SmaI         3272  CCCGGG               CCC^GGG
             3827  CCCGGG              
SphI          331  GCATGC               GCATG^C
             1193  GCATGC              
SspI         2265  AATATT               AAT^ATT
             4169  AATATT              
Van91I        483  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              561  CCAGCGTCTGG         
XhoII        1332  GGATCC               [GA]^GATC[CT]
             3104  AGATCT              
XmaCI        3272  CCCGGG               C^CCGGG
             3827  CCCGGG              
XmaI         3272  CCCGGG               C^CCGGG
             3827  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PstI        
PvuII       RsaI        SalI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XmnI        
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