Restriction map for pF1KB7007
 (Linear) (Six-base) MAPSORT of: KIBB7007.seq  Check: 8581  from: 1  to: 4272



KIBB7007.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     158     922    2006    2340    2655    2670    3290    4272 
   Size:        158     764    1084     334     315      15     620     982

  Fragments arranged by size: 

                1084     982     764     620     334     315     158      15
            
 
AccI GT'mk_AC

Cuts at:      0    1028    2858    3626    3860    4272 
   Size:       1028    1830     768     234     412

  Fragments arranged by size: 

                1830    1028     768     412     234
 
Acc65I G'GTAC_C

Cuts at:      0     950    4272 
   Size:        950    3322
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     838    1636    2507    3355    3364    3548    4272 
   Size:        305     533     798     871     848       9     184     724

  Fragments arranged by size: 

                 871     848     798     724     533     305     184       9
            
 
AfeI AGC'GCT

Cuts at:      0    2624    4272 
   Size:       2624    1648
 
AflIII A'CryG_T

Cuts at:      0    1172    1267    1971    2534    3196    4272 
   Size:       1172      95     704     563     662    1076

  Fragments arranged by size: 

                1172    1076     704     662     563      95
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3867    3954    4272 
   Size:       3867      87     318

  Fragments arranged by size: 

                3867     318      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     157     354    2254    4033    4272 
   Size:        157     197    1900    1779     239

  Fragments arranged by size: 

                1900    1779     239     197     157
 
AlwNI CAG_nnn'CTG

Cuts at:      0     315     399     489     567    1138    1666    2074    3481 
   Size:        315      84      90      78     571     528     408    1407

Cuts at:   3481    3744    4272 
   Size:        263     528

  Fragments arranged by size: 

                1407     571     528     528     408     315     263      90
                  84      78
 
ApaI G_GGCC'C

Cuts at:      0     670    1716    3273    3834    4272 
   Size:        670    1046    1557     561     438

  Fragments arranged by size: 

                1557    1046     670     561     438
 
ApaLI G'TGCA_C

Cuts at:      0     305    4272 
   Size:        305    3967
 
ApoI r'AATT_y

Cuts at:      0     365    1510    2176    2983    3358    3459    3643    4272 
   Size:        365    1145     666     807     375     101     184     629

  Fragments arranged by size: 

                1145     807     666     629     375     365     184     101
            
 
AvaI C'yCGr_G

Cuts at:      0     581    1389    2586    3272    3827    4272 
   Size:        581     808    1197     686     555     445

  Fragments arranged by size: 

                1197     808     686     581     555     445
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     942     975    1538    1571    4272 
   Size:        942      33     563      33    2701

  Fragments arranged by size: 

                2701     942     563      33      33
 
BamHI G'GATC_C

Cuts at:      0    1332    4272 
   Size:       1332    2940
 
BanI G'GyrC_C

Cuts at:      0     492     648     715     950    1461    2837    4272 
   Size:        492     156      67     235     511    1376    1435

  Fragments arranged by size: 

                1435    1376     511     492     235     156      67
 
BanII G_rGCy'C

Cuts at:      0     521     670    1366    1706    1716    1750    1893    1921 
   Size:        521     149     696     340      10      34     143      28

Cuts at:   1921    3273    3584    3797    3834    3980    4272 
   Size:       1352     311     213      37     146     292

  Fragments arranged by size: 

                1352     696     521     340     311     292     213     149
                 146     143      37      34      28      10
 
BbeI G_GCGC'C

Cuts at:      0     719    4272 
   Size:        719    3553
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34    1079    4272 
   Size:         34    1045    3193

  Fragments arranged by size: 

                3193    1045      34
 
BbvCI CC'TCA_GC

Cuts at:      0     392    3745    4034    4272 
   Size:        392    3353     289     238

  Fragments arranged by size: 

                3353     392     289     238
 
BfrBI ATG'CAT

Cuts at:      0    2113    4272 
   Size:       2113    2159
 
BglI GCCn_nnn'nGGC

Cuts at:      0     665    2603    4272 
   Size:        665    1938    1669

  Fragments arranged by size: 

                1938    1669     665
 
BglII A'GATC_T

Cuts at:      0    3104    4272 
   Size:       3104    1168
 
BlpI GC'TnA_GC

Cuts at:      0    1189    4272 
   Size:       1189    3083
 
Bme1580I G_kGCm'C

Cuts at:      0     169     309     670    1716    2840    2996    3273    3834 
   Size:        169     140     361    1046    1124     156     277     561

Cuts at:   3834    4272 
   Size:        438

  Fragments arranged by size: 

                1124    1046     561     438     361     277     169     156
                 140
 
BmgBI CAC'GTC

Cuts at:      0     797    2435    2447    2535    4272 
   Size:        797    1638      12      88    1737

  Fragments arranged by size: 

                1737    1638     797      88      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1086    1364    4272 
   Size:       1086     278    2908

  Fragments arranged by size: 

                2908    1086     278
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     314    2937    3705    3999    4272 
   Size:        314    2623     768     294     273

  Fragments arranged by size: 

                2623     768     314     294     273
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616    1018    1386    2141    2907    2990    3256    3274 
   Size:        616     402     368     755     766      83     266      18

Cuts at:   3274    4272 
   Size:        998

  Fragments arranged by size: 

                 998     766     755     616     402     368     266      83
                  18
 
Bpu10I CC'TnA_GC

Cuts at:      0     392    3745    3851    4034    4272 
   Size:        392    3353     106     183     238

  Fragments arranged by size: 

                3353     392     238     183     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1935    4272 
   Size:        724    1211    2337

  Fragments arranged by size: 

                2337    1211     724
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2508    4272 
   Size:       2508    1764
 
BsaAI yAC'GTr

Cuts at:      0    1268    4272 
   Size:       1268    3004
 
BsaBI GATnn'nnATC

Cuts at:      0    1523    4272 
   Size:       1523    2749
 
BsaHI Gr'CG_yC

Cuts at:      0     716    1019    4272 
   Size:        716     303    3253

  Fragments arranged by size: 

                3253     716     303
 
BsaWI w'CCGG_w

Cuts at:      0    2295    4272 
   Size:       2295    1977
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1215    1245    2709    2739    2944    2974    4067    4097 
   Size:       1215      30    1464      30     205      30    1093      30

Cuts at:   4097    4272 
   Size:        175

  Fragments arranged by size: 

                1464    1215    1093     205     175      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     240     721     979     982    1003    1374    1619 
   Size:        211      29     481     258       3      21     371     245

Cuts at:   1619    1774    3094    3253    3310    3328    3331    3334    3352 
   Size:        155    1320     159      57      18       3       3      18

Cuts at:   3352    3640    3795    3907    4272 
   Size:        288     155     112     365

  Fragments arranged by size: 

                1320     481     371     365     288     258     245     211
                 159     155     155     112      57      29      21      18
                  18       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      28     266    1734    2577    2619    4272 
   Size:         28     238    1468     843      42    1653

  Fragments arranged by size: 

                1653    1468     843     238      42      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     130    1925    1930    2445    2497    2747    2912 
   Size:         69      61    1795       5     515      52     250     165

Cuts at:   2912    2996    3221    3268    4272 
   Size:         84     225      47    1004

  Fragments arranged by size: 

                1795    1004     515     250     225     165      84      69
                  61      52      47       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     309     521    2918    3281    4272 
   Size:        309     212    2397     363     991

  Fragments arranged by size: 

                2397     991     363     309     212
 
BsmI GAATG_Cn'

Cuts at:      0    2367    4272 
   Size:       2367    1905
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     309     521     670    1366    1706    1716    1750 
   Size:        169     140     212     149     696     340      10      34

Cuts at:   1750    1893    1921    2840    2918    2996    3273    3281    3584 
   Size:        143      28     919      78      78     277       8     303

Cuts at:   3584    3797    3834    3980    4272 
   Size:        213      37     146     292

  Fragments arranged by size: 

                 919     696     340     303     292     277     213     212
                 169     149     146     143     140      78      78      37
                  34      28      10       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158     922    2006    2340    2655    2670    3290    4272 
   Size:        158     764    1084     334     315      15     620     982

  Fragments arranged by size: 

                1084     982     764     620     334     315     158      15
            
 
BsrDI GCAATG_nn'

Cuts at:      0    1883    2328    4272 
   Size:       1883     445    1944

  Fragments arranged by size: 

                1944    1883     445
 
BsrFI r'CCGG_y

Cuts at:      0     277    4272 
   Size:        277    3995
 
BssSI C'ACGA_G

Cuts at:      0     336     520    4272 
   Size:        336     184    3752

  Fragments arranged by size: 

                3752     336     184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2689    2963    4272 
   Size:       2689     274    1309

  Fragments arranged by size: 

                2689    1309     274
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2607    4272 
   Size:       2607    1665
 
BstYI r'GATC_y

Cuts at:      0    1332    3104    4272 
   Size:       1332    1772    1168

  Fragments arranged by size: 

                1772    1332    1168
 
BtgI C'CryG_G

Cuts at:      0     119     185    1965    2600    4272 
   Size:        119      66    1780     635    1672

  Fragments arranged by size: 

                1780    1672     635     119      66
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1253    4272 
   Size:       1253    3019
 
BtsI GCAGTG_nn'

Cuts at:      0     288     357     924    1942    2823    4272 
   Size:        288      69     567    1018     881    1449

  Fragments arranged by size: 

                1449    1018     881     567     288      69
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     778     813    4272 
   Size:        778      35    3459

  Fragments arranged by size: 

                3459     778      35
 
DraI TTT'AAA

Cuts at:      0    1089    4272 
   Size:       1089    3183
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1024    1288    4272 
   Size:       1024     264    2984

  Fragments arranged by size: 

                2984    1024     264
 
EaeI y'GGCC_r

Cuts at:      0     635    1559    1902    2429    2777    4272 
   Size:        635     924     343     527     348    1495

  Fragments arranged by size: 

                1495     924     635     527     348     343
 
EarI CTCTTCn'nnn_

Cuts at:      0    1003    2123    3329    3549    4272 
   Size:       1003    1120    1206     220     723

  Fragments arranged by size: 

                1206    1120    1003     723     220
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     394     598    4272 
   Size:        394     204    3674

  Fragments arranged by size: 

                3674     394     204
 
EcoICRI GAG'CTC

Cuts at:      0     519    4272 
   Size:        519    3753
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     616     838    1018    1386    1636    2141    2507 
   Size:        305     311     222     180     368     250     505     366

Cuts at:   2507    2907    2990    3256    3274    3355    3364    3548    4272 
   Size:        400      83     266      18      81       9     184     724

  Fragments arranged by size: 

                 724     505     400     368     366     311     305     266
                 250     222     184     180      83      81      18       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     703     724    2525    3292    3655    4272 
   Size:        703      21    1801     767     363     617

  Fragments arranged by size: 

                1801     767     703     617     363      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     666    1712    1713    3269    4272 
   Size:        543     123    1046       1    1556    1003

  Fragments arranged by size: 

                1556    1046    1003     543     123       1
 
EcoRI G'AATT_C

Cuts at:      0    2983    4272 
   Size:       2983    1289
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     256    1250    3072    4272 
   Size:        256     994    1822    1200

  Fragments arranged by size: 

                1822    1200     994     256
 
FspI TGC'GCA

Cuts at:      0     976    4272 
   Size:        976    3296
 
HaeII r_GCGC'y

Cuts at:      0     719    2626    2801    4272 
   Size:        719    1907     175    1471

  Fragments arranged by size: 

                1907    1471     719     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1441    1473    2742    2774    2893    2925    3091    3123 
   Size:       1441      32    1269      32     119      32     166      32

Cuts at:   3123    3802    3834    3853    3885    3940    3972    4067    4099 
   Size:        679      32      19      32      55      32      95      32

Cuts at:   4099    4208    4240    4272 
   Size:        109      32      32

  Fragments arranged by size: 

                1441    1269     679     166     119     109      95      55
                  32      32      32      32      32      32      32      32
                  32      32      19
 
HincII GTy'rAC

Cuts at:      0    1029    1067    1118    3129    3627    3861    3906    4086 
   Size:       1029      38      51    2011     498     234      45     180

Cuts at:   4086    4272 
   Size:        186

  Fragments arranged by size: 

                2011    1029     498     234     186     180      51      45
                  38
 
HindIII A'AGCT_T

Cuts at:      0    1853    3049    4272 
   Size:       1853    1196    1223

  Fragments arranged by size: 

                1853    1223    1196
 
HpaI GTT'AAC

Cuts at:      0    1067    1118    4272 
   Size:       1067      51    3154

  Fragments arranged by size: 

                3154    1067      51
 
KasI G'GCGC_C

Cuts at:      0     715    4272 
   Size:        715    3557
 
KpnI G_GTAC'C

Cuts at:      0     954    4272 
   Size:        954    3318
 
MfeI C'AATT_G

Cuts at:      0    4094    4272 
   Size:       4094     178
 
MluI A'CGCG_T

Cuts at:      0    3196    4272 
   Size:       3196    1076
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3422    4272 
   Size:       3422     850
 
MscI TGG'CCA

Cuts at:      0    1561    1904    2431    4272 
   Size:       1561     343     527    1841

  Fragments arranged by size: 

                1841    1561     527     343
 
MslI CAynn'nnrTG

Cuts at:      0     336    1964    1970    4272 
   Size:        336    1628       6    2302

  Fragments arranged by size: 

                2302    1628     336       6
 
MspA1I CmG'CkG

Cuts at:      0     399     465     555     624    2843    2906    3010    3366 
   Size:        399      66      90      69    2219      63     104     356

Cuts at:   3366    3933    4272 
   Size:        567     339

  Fragments arranged by size: 

                2219     567     399     356     339     104      90      69
                  66      63
 
NaeI GCC'GGC

Cuts at:      0     279    4272 
   Size:        279    3993
 
NarI GG'CG_CC

Cuts at:      0     716    4272 
   Size:        716    3556
 
NcoI C'CATG_G

Cuts at:      0     119    1965    4272 
   Size:        119    1846    2307

  Fragments arranged by size: 

                2307    1846     119
 
NdeI CA'TA_TG

Cuts at:      0    3566    4272 
   Size:       3566     706
 
NgoMIV G'CCGG_C

Cuts at:      0     277    4272 
   Size:        277    3995
 
NsiI A_TGCA'T

Cuts at:      0    2115    4272 
   Size:       2115    2157
 
NspI r_CATG'y

Cuts at:      0     335    1065    1176    1197    1975    3450    4272 
   Size:        335     730     111      21     778    1475     822

  Fragments arranged by size: 

                1475     822     778     730     335     111      21
 
PasI CC'CwG_GG

Cuts at:      0    1708    4272 
   Size:       1708    2564
 
PciI A'CATG_T

Cuts at:      0    1172    1971    4272 
   Size:       1172     799    2301

  Fragments arranged by size: 

                2301    1172     799
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     489     567    4272 
   Size:        489      78    3705

  Fragments arranged by size: 

                3705     489      78
 
PfoI T'CCnGG_A

Cuts at:      0    3290    4272 
   Size:       3290     982
 
PmlI CAC'GTG

Cuts at:      0    1268    4272 
   Size:       1268    3004
 
PpuMI rG'GwC_Cy

Cuts at:      0     543    4272 
   Size:        543    3729
 
PshAI GACnn'nnGTC

Cuts at:      0    1247    4272 
   Size:       1247    3025
 
PsiI TTA'TAA

Cuts at:      0    2182    3163    4272 
   Size:       2182     981    1109

  Fragments arranged by size: 

                2182    1109     981
 
PspOMI G'GGCC_C

Cuts at:      0     666    1712    3269    3830    4272 
   Size:        666    1046    1557     561     442

  Fragments arranged by size: 

                1557    1046     666     561     442
 
PstI C_TGCA'G

Cuts at:      0     352     404     665     919     947    1249    2337    2818 
   Size:        352      52     261     254      28     302    1088     481

Cuts at:   2818    3618    4272 
   Size:        800     654

  Fragments arranged by size: 

                1088     800     654     481     352     302     261     254
                  52      28
 
PvuII CAG'CTG

Cuts at:      0     399     555     624    2906    3010    3933    4272 
   Size:        399     156      69    2282     104     923     339

  Fragments arranged by size: 

                2282     923     399     339     156     104      69
 
RsrII CG'GwC_CG

Cuts at:      0     381    4272 
   Size:        381    3891
 
SacI G_AGCT'C

Cuts at:      0     521    4272 
   Size:        521    3751
 
SalI G'TCGA_C

Cuts at:      0    1027    3625    3859    4272 
   Size:       1027    2598     234     413

  Fragments arranged by size: 

                2598    1027     413     234
 
SanDI GG'GwC_CC

Cuts at:      0     543    4272 
   Size:        543    3729
 
SbfI CC_TGCA'GG

Cuts at:      0     665    4272 
   Size:        665    3607
 
ScaI AGT'ACT

Cuts at:      0    2962    4272 
   Size:       2962    1310
 
SfcI C'TryA_G

Cuts at:      0     348     400     661     915     943    1218    1245    2333 
   Size:        348      52     261     254      28     275      27    1088

Cuts at:   2333    2814    3614    4272 
   Size:        481     800     658

  Fragments arranged by size: 

                1088     800     658     481     348     275     261     254
                  52      28      27
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     665    4272 
   Size:        665    3607
 
SfoI GGC'GCC

Cuts at:      0     717    4272 
   Size:        717    3555
 
SmaI CCC'GGG

Cuts at:      0    3274    3829    4272 
   Size:       3274     555     443

  Fragments arranged by size: 

                3274     555     443
 
SmlI C'TyrA_G

Cuts at:      0     581     703    1914    4272 
   Size:        581     122    1211    2358

  Fragments arranged by size: 

                2358    1211     581     122
 
SphI G_CATG'C

Cuts at:      0     335    1197    4272 
   Size:        335     862    3075

  Fragments arranged by size: 

                3075     862     335
 
SspI AAT'ATT

Cuts at:      0    2267    4171    4272 
   Size:       2267    1904     101

  Fragments arranged by size: 

                2267    1904     101
 
StuI AGG'CCT

Cuts at:      0     346    4272 
   Size:        346    3926
 
StyI C'CwwG_G

Cuts at:      0     119     128     638     753    1965    3656    3800    4272 
   Size:        119       9     510     115    1212    1691     144     472

  Fragments arranged by size: 

                1691    1212     510     472     144     119     115       9
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     150     292    4272 
   Size:        150     142    3980

  Fragments arranged by size: 

                3980     150     142
 
TatI w'GTAC_w

Cuts at:      0    2960    4272 
   Size:       2960    1312
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1215    1247    2186    2218    3541    3573    4272 
   Size:       1215      32     939      32    1323      32     699

  Fragments arranged by size: 

                1323    1215     939     699      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3622    3840    4081    4272 
   Size:       3622     218     241     191

  Fragments arranged by size: 

                3622     241     218     191
 
XhoI C'TCGA_G

Cuts at:      0     581    4272 
   Size:        581    3691
 
XmaI C'CCGG_G

Cuts at:      0    3272    3827    4272 
   Size:       3272     555     445

  Fragments arranged by size: 

                3272     555     445
 
XmnI GAAnn'nnTTC

Cuts at:      0     784    1150    2303    3050    4272 
   Size:        784     366    1153     747    1222

  Fragments arranged by size: 

                1222    1153     784     747     366
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BssSI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI     PstI
    PvuII    RsrII     SacI     SalI    SanDI     SbfI     ScaI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AleI     AloI     AscI     AseI
    AsiSI    AvrII     BcgI    BciVI     BclI     BmtI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI   DraIII     EagI    EcoRV     FseI    FspAI
     NheI     NotI     NruI     PacI     PmeI     PpiI    PspXI     PsrI
     PvuI    SacII     SapI    SexAI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI     XbaI     XcmI     ZraI
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