Restriction map for
pF1KB6008
(Circular) (Six-base) MAPSORT of: pF1KB6008.seq Check: 4889 from: 1 to: 11552
pF1KB6008 11552 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1222 3250 3379 3567 7192 7387 7471 7920 7984
Size: 2028 129 188 3625 195 84 449 64
Cuts at: 7984 8257 8445 1222
Size: 273 188 4329
Fragments arranged by size:
4329 3625 2028 449 273 195 188 188
129 84 64
AatII G_ACGT'C
Cuts at: 1520 1873 3308 5387 5516 5783 1520
Size: 353 1435 2079 129 267 7289
Fragments arranged by size:
7289 2079 1435 353 267 129
AccI GT'mk_AC
Cuts at: 4919 6731 8590 4919
Size: 1812 1859 7881
Fragments arranged by size:
7881 1859 1812
Acc65I G'GTAC_C
Cuts at: 406 4856 8568 406
Size: 4450 3712 3390
Fragments arranged by size:
4450 3712 3390
AclI AA'CG_TT
Cuts at: 6230 11392 6230
Size: 5162 6390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 811 1308 1501 2477 3079 3707 4015 4197
Size: 176 497 193 976 602 628 308 182
Cuts at: 4197 4253 4339 4514 4746 5438 5863 6878 8110
Size: 56 86 175 232 692 425 1015 1232
Cuts at: 8110 8351 9377 9809 10556 10824 635
Size: 241 1026 432 747 268 1363
Fragments arranged by size:
1363 1232 1026 1015 976 747 692 628
602 497 432 425 308 268 241 232
193 182 176 175 86 56
AfeI AGC'GCT
Cuts at: 1174 1174
Size: 11552
AflIII A'CryG_T
Cuts at: 1940 3434 3574 7015 8260 8904 10008 10985 1940
Size: 1494 140 3441 1245 644 1104 977 2507
Fragments arranged by size:
3441 2507 1494 1245 1104 977 644 140
AgeI A'CCGG_T
Cuts at: 9940 9940
Size: 11552
AhdI GACnn_n'nnGTC
Cuts at: 6353 7638 6353
Size: 1285 10267
AleI CACnn'nnGTG
Cuts at: 802 3437 5293 802
Size: 2635 1856 7061
Fragments arranged by size:
7061 2635 1856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 315 4267 5178 7077 315
Size: 3952 911 1899 4790
Fragments arranged by size:
4790 3952 1899 911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4591 4623 7121 7153 4591
Size: 32 2498 32 8990
Fragments arranged by size:
8990 2498 32 32
AlwNI CAG_nnn'CTG
Cuts at: 789 1040 1050 1116 1344 1791 4317 5022 5175
Size: 251 10 66 228 447 2526 705 153
Cuts at: 5175 5542 6057 6909 8857 10424 789
Size: 367 515 852 1948 1567 1917
Fragments arranged by size:
2526 1948 1917 1567 852 705 515 447
367 251 228 153 66 10
ApaI G_GGCC'C
Cuts at: 4361 4546 4577 5002 7880 4361
Size: 185 31 425 2878 8033
Fragments arranged by size:
8033 2878 425 185 31
ApaLI G'TGCA_C
Cuts at: 1425 3554 5955 7458 7905 10322 1425
Size: 2129 2401 1503 447 2417 2655
Fragments arranged by size:
2655 2417 2401 2129 1503 447
ApoI r'AATT_y
Cuts at: 376 3099 6000 8237 8556 10905 11161 376
Size: 2723 2901 2237 319 2349 256 767
Fragments arranged by size:
2901 2723 2349 2237 767 319 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11552
AvaI C'yCGr_G
Cuts at: 835 1199 1352 1739 2464 2541 2764 3337 4545
Size: 364 153 387 725 77 223 573 1208
Cuts at: 4545 5081 5773 7069 7782 7879 8572 835
Size: 536 692 1296 713 97 693 3815
Fragments arranged by size:
3815 1296 1208 725 713 693 692 573
536 387 364 223 153 97 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 264 297 264
Size: 33 11519
BamHI G'GATC_C
Cuts at: 2825 8577 2825
Size: 5752 5800
BanI G'GyrC_C
Cuts at: 406 1186 1978 3840 4856 4905 4983 5467 6079
Size: 780 792 1862 1016 49 78 484 612
Cuts at: 6079 6238 7586 8245 8568 8982 9230 9265 406
Size: 159 1348 659 323 414 248 35 2693
Fragments arranged by size:
2693 1862 1348 1016 792 780 659 612
484 414 323 248 159 78 49 35
BanII G_rGCy'C
Cuts at: 684 1739 2186 2465 2750 3193 3868 4100 4277
Size: 1055 447 279 285 443 675 232 177
Cuts at: 4277 4361 4546 4553 4577 5002 6784 7880 8450
Size: 84 185 7 24 425 1782 1096 570
Cuts at: 8450 8566 9596 684
Size: 116 1030 2640
Fragments arranged by size:
2640 1782 1096 1055 1030 675 570 447
443 425 285 279 232 185 177 116
84 24 7
BbeI G_GCGC'C
Cuts at: 1982 4909 8986 9234 1982
Size: 2927 4077 248 4300
Fragments arranged by size:
4300 4077 2927 248
BbsI GAAGACnn'nnnn_
Cuts at: 868 1669 2270 3161 3300 3695 3936 5678 7267
Size: 801 601 891 139 395 241 1742 1589
Cuts at: 7267 7634 7891 7975 868
Size: 367 257 84 4445
Fragments arranged by size:
4445 1742 1589 891 801 601 395 367
257 241 139 84
BbvCI CC'TCA_GC
Cuts at: 1582 2365 5875 6400 6958 7809 8216 1582
Size: 783 3510 525 558 851 407 4918
Fragments arranged by size:
4918 3510 851 783 558 525 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 235 3400 3434 8272 8306 201
Size: 34 3165 34 4838 34 3447
Fragments arranged by size:
4838 3447 3165 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 265 9450 10211 11552 265
Size: 9185 761 1341 265
Fragments arranged by size:
9185 1341 761 265
BclI T'GATC_A
Cuts at: 9072 9072
Size: 11552
BfrBI ATG'CAT
Cuts at: 5463 6249 5463
Size: 786 10766
BglI GCCn_nnn'nGGC
Cuts at: 1976 2069 2162 2543 5488 5636 7784 1976
Size: 93 93 381 2945 148 2148 5744
Fragments arranged by size:
5744 2945 2148 381 148 93 93
BglII A'GATC_T
Cuts at: 2249 6944 9067 11104 2249
Size: 4695 2123 2037 2697
Fragments arranged by size:
4695 2697 2123 2037
BlpI GC'TnA_GC
Cuts at: 4426 5371 6322 7795 8664 4426
Size: 945 951 1473 869 7314
Fragments arranged by size:
7314 1473 951 945 869
Bme1580I G_kGCm'C
Cuts at: 936 994 1429 2216 2230 3444 3487 3558 4361
Size: 58 435 787 14 1214 43 71 803
Cuts at: 4361 4546 4577 4988 5002 5890 5959 6141 7462
Size: 185 31 411 14 888 69 182 1321
Cuts at: 7462 7880 7909 9177 9270 10326 936
Size: 418 29 1268 93 1056 2162
Fragments arranged by size:
2162 1321 1268 1214 1056 888 803 787
435 418 411 185 182 93 71 69
58 43 31 29 14 14
BmgBI CAC'GTC
Cuts at: 1941 4716 5181 5334 6473 1941
Size: 2775 465 153 1139 7020
Fragments arranged by size:
7020 2775 1139 465 153
BmrI ACTGGGnnnn_n'
Cuts at: 979 1620 2217 3749 4850 7087 7108 7260 7500
Size: 641 597 1532 1101 2237 21 152 240
Cuts at: 7500 7632 7659 8764 8845 9180 11324 979
Size: 132 27 1105 81 335 2144 1207
Fragments arranged by size:
2237 2144 1532 1207 1105 1101 641 597
335 240 152 132 81 27 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 373 3909 9919 373
Size: 3536 6010 2006
Fragments arranged by size:
6010 3536 2006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 934 2179 2518 2642 3486 3626 3948 4465
Size: 24 1245 339 124 844 140 322 517
Cuts at: 4465 4988 5044 5190 5644 6079 6113 6508 6557
Size: 523 56 146 454 435 34 395 49
Cuts at: 6557 6705 6806 7123 7783 7978 8092 910
Size: 148 101 317 660 195 114 4370
Fragments arranged by size:
4370 1245 844 660 523 517 454 435
395 339 322 317 195 148 146 140
124 114 101 56 49 34 24
Bpu10I CC'TnA_GC
Cuts at: 1582 2365 3040 3587 3764 5875 6400 6958 7809
Size: 783 675 547 177 2111 525 558 851
Cuts at: 7809 8216 1582
Size: 407 4918
Fragments arranged by size:
4918 2111 851 783 675 558 547 525
407 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4357 8728 9607 10099 10397 4084
Size: 273 4371 879 492 298 5239
Fragments arranged by size:
5239 4371 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 5914 6198 11256 5914
Size: 284 5058 6210
Fragments arranged by size:
6210 5058 284
BsaAI yAC'GTr
Cuts at: 2705 3435 3575 6207 6953 7016 8905 2705
Size: 730 140 2632 746 63 1889 5352
Fragments arranged by size:
5352 2632 1889 746 730 140 63
BsaBI GATnn'nnATC
Cuts at: 6 9066 11103 6
Size: 9060 2037 455
Fragments arranged by size:
9060 2037 455
BsaHI Gr'CG_yC
Cuts at: 1517 1870 1979 3305 4906 5384 5513 5780 8983
Size: 353 109 1326 1601 478 129 267 3203
Cuts at: 8983 9231 9933 11429 1517
Size: 248 702 1496 1640
Fragments arranged by size:
3203 1640 1601 1496 1326 702 478 353
267 248 129 109
BsaWI w'CCGG_w
Cuts at: 496 1165 2666 4106 4930 5119 8741 9262 9940
Size: 669 1501 1440 824 189 3622 521 678
Cuts at: 9940 10214 10361 10772 496
Size: 274 147 411 1276
Fragments arranged by size:
3622 1501 1440 1276 824 678 669 521
411 274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1360 1390 1930 1960 2615 2645 2761 2791 3067
Size: 30 540 30 655 30 116 30 276
Cuts at: 3067 3097 3358 3388 3508 3538 3821 3851 4063
Size: 30 261 30 120 30 283 30 212
Cuts at: 4063 4093 6276 6306 6481 6511 6610 6640 6766
Size: 30 2183 30 175 30 99 30 126
Cuts at: 6766 6784 6796 6814 7121 7151 8260 8290 8404
Size: 18 12 18 307 30 1109 30 114
Cuts at: 8404 8434 1360
Size: 30 4478
Fragments arranged by size:
4478 2183 1109 655 540 307 283 276
261 212 175 126 120 116 114 99
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 710 735 1864 2915 3514 3951 4493 4687 5193
Size: 25 1129 1051 599 437 542 194 506
Cuts at: 5193 6505 6523 7216 7456 7735 8084 8165 8281
Size: 1312 18 693 240 279 349 81 116
Cuts at: 8281 710
Size: 3981
Fragments arranged by size:
3981 1312 1129 1051 693 599 542 506
437 349 279 240 194 116 81 25
18
BseYI C'CCAG_C
Cuts at: 478 724 880 3003 3443 5214 5407 5609 5631
Size: 246 156 2123 440 1771 193 202 22
Cuts at: 5631 7757 8977 9735 10312 10808 478
Size: 2126 1220 758 577 496 1222
Fragments arranged by size:
2126 2123 1771 1222 1220 758 577 496
440 246 202 193 156 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 999 1036 1444 1756 2664 2809 3628 5035
Size: 805 37 408 312 908 145 819 1407
Cuts at: 5035 5138 6139 6475 6852 6933 6963 7188 7449
Size: 103 1001 336 377 81 30 225 261
Cuts at: 7449 7948 7983 8428 8449 8454 8559 194
Size: 499 35 445 21 5 105 3187
Fragments arranged by size:
3187 1407 1001 908 819 805 499 445
408 377 336 312 261 225 145 105
103 81 37 35 30 21 5
BsiEI CG_ry'CG
Cuts at: 104 1962 2717 4117 4227 7543 9094 9140 10348
Size: 1858 755 1400 110 3316 1551 46 1208
Cuts at: 10348 104
Size: 1308
Fragments arranged by size:
3316 1858 1551 1400 1308 1208 755 110
46
BsiHKAI G_wGCw'C
Cuts at: 799 1144 1429 1948 2710 3193 3558 3770 5725
Size: 345 285 519 762 483 365 212 1955
Cuts at: 5725 5959 6784 7462 7513 7909 8422 8566 9344
Size: 234 825 678 51 396 513 144 778
Cuts at: 9344 9534 10326 799
Size: 190 792 2025
Fragments arranged by size:
2025 1955 825 792 778 762 678 519
513 483 396 365 345 285 234 212
190 144 51
BsmI GAATG_Cn'
Cuts at: 1161 2146 6559 7056 1161
Size: 985 4413 497 5657
Fragments arranged by size:
5657 4413 985 497
BsmBI CGTCTCn'nnnn_
Cuts at: 5065 5290 5520 7752 8095 5065
Size: 225 230 2232 343 8522
Fragments arranged by size:
8522 2232 343 230 225
Bsp1286I G_dGCh'C
Cuts at: 684 799 936 994 1144 1429 1739 1948 2186
Size: 115 137 58 150 285 310 209 238
Cuts at: 2186 2216 2230 2465 2710 2750 3193 3444 3487
Size: 30 14 235 245 40 443 251 43
Cuts at: 3487 3558 3770 3868 4100 4277 4361 4546 4553
Size: 71 212 98 232 177 84 185 7
Cuts at: 4553 4577 4988 5002 5725 5890 5959 6141 6784
Size: 24 411 14 723 165 69 182 643
Cuts at: 6784 7462 7513 7880 7909 8422 8450 8566 9177
Size: 678 51 367 29 513 28 116 611
Cuts at: 9177 9270 9344 9534 9596 10326 684
Size: 93 74 190 62 730 1910
Fragments arranged by size:
1910 730 723 678 643 611 513 443
411 367 310 285 251 245 238 235
232 212 209 190 185 182 177 165
150 137 116 115 98 93 84 74
71 69 62 58 51 43 40 30
29 28 24 14 14 7
BspEI T'CCGG_A
Cuts at: 1165 5119 10772 1165
Size: 3954 5653 1945
Fragments arranged by size:
5653 3954 1945
BspHI T'CATG_A
Cuts at: 3257 8081 10728 3257
Size: 4824 2647 4081
Fragments arranged by size:
4824 4081 2647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 589 1048 1222 1352 1607 1675 1799 1928
Size: 407 459 174 130 255 68 124 129
Cuts at: 1928 2090 2119 2456 3250 3379 3567 3682 5950
Size: 162 29 337 794 129 188 115 2268
Cuts at: 5950 7115 7192 7387 7471 7920 7921 7984 8257
Size: 1165 77 195 84 449 1 63 273
Cuts at: 8257 8445 8602 8816 9118 9499 182
Size: 188 157 214 302 381 2235
Fragments arranged by size:
2268 2235 1165 794 459 449 407 381
337 302 273 255 214 195 188 188
174 162 157 130 129 129 124 115
84 77 68 63 29 1
BsrBI CCG'CTC
Cuts at: 50 1433 2117 4023 9844 9898 11382 11547 50
Size: 1383 684 1906 5821 54 1484 165 55
Fragments arranged by size:
5821 1906 1484 1383 684 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1254 2151 3416 4133 9464 1254
Size: 897 1265 717 5331 3342
Fragments arranged by size:
5331 3342 1265 897 717
BsrFI r'CCGG_y
Cuts at: 2984 3716 9550 9731 9940 2984
Size: 732 5834 181 209 4596
Fragments arranged by size:
5834 4596 732 209 181
BsrGI T'GTAC_A
Cuts at: 257 257
Size: 11552
BssSI C'ACGA_G
Cuts at: 9823 10181 9823
Size: 358 11194
BstAPI GCAn_nnn'nTGC
Cuts at: 734 1214 1498 4800 5545 6875 7245 7913 7985
Size: 480 284 3302 745 1330 370 668 72
Cuts at: 7985 8929 734
Size: 944 3357
Fragments arranged by size:
3357 3302 1330 944 745 668 480 370
284 72
BstBI TT'CG_AA
Cuts at: 9913 9913
Size: 11552
BstEII G'GTnAC_C
Cuts at: 1540 2880 3459 4743 4891 4926 5688 1540
Size: 1340 579 1284 148 35 762 7404
Fragments arranged by size:
7404 1340 1284 762 579 148 35
BstXI CCAn_nnnn'nTGG
Cuts at: 2892 4596 4995 5827 8457 2892
Size: 1704 399 832 2630 5987
Fragments arranged by size:
5987 2630 1704 832 399
BstYI r'GATC_y
Cuts at: 1 1238 1331 2249 2825 4379 4808 5135 5618
Size: 1237 93 918 576 1554 429 327 483
Cuts at: 5618 6365 6944 8577 9044 9067 9402 9648 10660
Size: 747 579 1633 467 23 335 246 1012
Cuts at: 10660 10746 10758 11104 1
Size: 86 12 346 449
Fragments arranged by size:
1633 1554 1237 1012 918 747 579 576
483 467 449 429 346 335 327 246
93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1103 3729 1103
Size: 2626 8926
BtgI C'CryG_G
Cuts at: 200 1502 1620 1997 2417 3020 3050 4058 4589
Size: 1302 118 377 420 603 30 1008 531
Cuts at: 4589 4748 5338 5684 6998 7624 8510 200
Size: 159 590 346 1314 626 886 3242
Fragments arranged by size:
3242 1314 1302 1008 886 626 603 590
531 420 377 346 159 118 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1092 2368 4230 7466 9502 9683 9846 1092
Size: 1276 1862 3236 2036 181 163 2798
Fragments arranged by size:
3236 2798 2036 1862 1276 181 163
BtsI GCAGTG_nn'
Cuts at: 696 784 1021 2092 3202 3616 3633 3768 4437
Size: 88 237 1071 1110 414 17 135 669
Cuts at: 4437 6502 6519 8288 8466 8795 696
Size: 2065 17 1769 178 329 3453
Fragments arranged by size:
3453 2065 1769 1110 1071 669 414 329
237 178 135 88 17 17
ClaI AT'CG_AT
Cuts at: 527 7898 527
Size: 7371 4181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5474 5509 7341 7376 5474
Size: 35 1832 35 9650
Fragments arranged by size:
9650 1832 35 35
DraI TTT'AAA
Cuts at: 8551 8551
Size: 11552
DraIII CAC_nnn'GTG
Cuts at: 1148 2291 3482 5811 5982 6875 1148
Size: 1143 1191 2329 171 893 5825
Fragments arranged by size:
5825 2329 1191 1143 893 171
DrdI GACnn_nn'nnGTC
Cuts at: 430 9258 10116 430
Size: 8828 858 1866
Fragments arranged by size:
8828 1866 858
EaeI y'GGCC_r
Cuts at: 518 1509 1617 1959 1985 2982 3473 3714 4055
Size: 991 108 342 26 997 491 241 341
Cuts at: 4055 4262 5829 6460 7001 9137 9311 9702 9729
Size: 207 1567 631 541 2136 174 391 27
Cuts at: 9729 11074 518
Size: 1345 996
Fragments arranged by size:
2136 1567 1345 997 996 991 631 541
491 391 342 341 241 207 174 108
27 26
EagI C'GGCC_G
Cuts at: 1959 9137 1959
Size: 7178 4374
EarI CTCTTCn'nnn_
Cuts at: 787 2932 5233 5761 5839 5912 6854 7645 8164
Size: 2145 2301 528 78 73 942 791 519
Cuts at: 8164 9575 9785 787
Size: 1411 210 2554
Fragments arranged by size:
2554 2301 2145 1411 942 791 528 519
210 78 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 4372 6981 7033 9760 10070 10216 11362 1747
Size: 2625 2609 52 2727 310 146 1146 1937
Fragments arranged by size:
2727 2625 2609 1937 1146 310 146 52
EcoICRI GAG'CTC
Cuts at: 3191 6782 8564 3191
Size: 3591 1782 6179
Fragments arranged by size:
6179 3591 1782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 811 910 934 1308 1501 2179 2477 2518
Size: 176 99 24 374 193 678 298 41
Cuts at: 2518 2642 3079 3486 3626 3707 3948 4015 4197
Size: 124 437 407 140 81 241 67 182
Cuts at: 4197 4253 4339 4465 4514 4746 4988 5044 5190
Size: 56 86 126 49 232 242 56 146
Cuts at: 5190 5438 5644 5863 6079 6113 6508 6557 6705
Size: 248 206 219 216 34 395 49 148
Cuts at: 6705 6806 6878 7123 7783 7978 8092 8110 8351
Size: 101 72 245 660 195 114 18 241
Cuts at: 8351 9377 9809 10556 10824 635
Size: 1026 432 747 268 1363
Fragments arranged by size:
1363 1026 747 678 660 437 432 407
395 374 298 268 248 245 242 241
241 232 219 216 206 195 193 182
176 148 146 140 126 124 114 101
99 86 81 72 67 56 56 49
49 41 34 24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2893 5059 2893
Size: 2166 9386
EcoO109I rG'GnC_Cy
Cuts at: 164 641 765 1037 1596 1788 2093 2816 4314
Size: 477 124 272 559 192 305 723 1498
Cuts at: 4314 4358 4401 4573 4999 5278 5404 6054 6528
Size: 44 43 172 426 279 126 650 474
Cuts at: 6528 6564 6921 7767 8691 164
Size: 36 357 846 924 3025
Fragments arranged by size:
3025 1498 924 846 723 650 559 477
474 426 357 305 279 272 192 172
126 124 44 43 36
EcoRI G'AATT_C
Cuts at: 8556 8556
Size: 11552
EcoRV GAT'ATC
Cuts at: 8346 8346
Size: 11552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 9571 9571
Size: 11552
FspI TGC'GCA
Cuts at: 4030 9333 4030
Size: 5303 6249
HaeII r_GCGC'y
Cuts at: 1176 1982 3543 4909 8986 9234 10256 11235 1176
Size: 806 1561 1366 4077 248 1022 979 1493
Fragments arranged by size:
4077 1561 1493 1366 1022 979 806 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1930 1962 2257 2289 3358 3390 3946 3978 4759
Size: 32 295 32 1069 32 556 32 781
Cuts at: 4759 4791 5188 5220 6339 6371 6784 6816 7624
Size: 32 397 32 1119 32 413 32 808
Cuts at: 7624 7656 8063 8095 8404 8436 9394 9424 9426
Size: 32 407 32 309 32 958 30 2
Cuts at: 9426 9456 1930
Size: 30 4026
Fragments arranged by size:
4026 1119 1069 958 808 781 556 413
407 397 309 295 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 237 3681 3798 5859 6228 6681 6975 7305 8591
Size: 3444 117 2061 369 453 294 330 1286
Cuts at: 8591 237
Size: 3198
Fragments arranged by size:
3444 3198 2061 1286 453 369 330 294
117
HindIII A'AGCT_T
Cuts at: 2343 2343
Size: 11552
KasI G'GCGC_C
Cuts at: 1978 4905 8982 9230 1978
Size: 2927 4077 248 4300
Fragments arranged by size:
4300 4077 2927 248
KpnI G_GTAC'C
Cuts at: 410 4860 8572 410
Size: 4450 3712 3390
Fragments arranged by size:
4450 3712 3390
MluI A'CGCG_T
Cuts at: 10985 10985
Size: 11552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5582 5669 6336 6640 6818 8458 10223 10407 5582
Size: 87 667 304 178 1640 1765 184 6727
Fragments arranged by size:
6727 1765 1640 667 304 184 178 87
MscI TGG'CCA
Cuts at: 520 1511 1987 4264 5831 9313 520
Size: 991 476 2277 1567 3482 2759
Fragments arranged by size:
3482 2759 2277 1567 991 476
MslI CAynn'nnrTG
Cuts at: 802 1058 2509 2693 2890 3049 3437 3788 4978
Size: 256 1451 184 197 159 388 351 1190
Cuts at: 4978 4993 5293 5332 6077 6124 6169 6767 7471
Size: 15 300 39 745 47 45 598 704
Cuts at: 7471 8455 9541 9668 802
Size: 984 1086 127 2686
Fragments arranged by size:
2686 1451 1190 1086 984 745 704 598
388 351 300 256 197 184 159 127
47 45 39 15
MspA1I CmG'CkG
Cuts at: 536 815 1097 1622 1911 1999 2048 2440 3551
Size: 279 282 525 289 88 49 392 1111
Cuts at: 3551 4686 4797 5394 5542 5936 6020 6459 6848
Size: 1135 111 597 148 394 84 439 389
Cuts at: 6848 6962 7535 7626 7761 7794 7919 8663 8977
Size: 114 573 91 135 33 125 744 314
Cuts at: 8977 9337 10350 10595 536
Size: 360 1013 245 1493
Fragments arranged by size:
1493 1135 1111 1013 744 597 573 525
439 394 392 389 360 314 289 282
279 245 148 135 125 114 111 91
88 84 49 33
NaeI GCC'GGC
Cuts at: 3718 9733 3718
Size: 6015 5537
NarI GG'CG_CC
Cuts at: 1979 4906 8983 9231 1979
Size: 2927 4077 248 4300
Fragments arranged by size:
4300 4077 2927 248
NcoI C'CATG_G
Cuts at: 1502 2417 4589 5338 5684 8510 1502
Size: 915 2172 749 346 2826 4544
Fragments arranged by size:
4544 2826 2172 915 749 346
NgoMIV G'CCGG_C
Cuts at: 3716 9731 3716
Size: 6015 5537
NsiI A_TGCA'T
Cuts at: 5465 6251 5465
Size: 786 10766
NspI r_CATG'y
Cuts at: 258 7857 8264 8605 9105 10012 258
Size: 7599 407 341 500 907 1798
Fragments arranged by size:
7599 1798 907 500 407 341
PasI CC'CwG_GG
Cuts at: 2179 2812 4569 7992 2179
Size: 633 1757 3423 5739
Fragments arranged by size:
5739 3423 1757 633
PciI A'CATG_T
Cuts at: 8260 10008 8260
Size: 1748 9804
PflMI CCAn_nnn'nTGG
Cuts at: 677 825 5175 6425 6909 677
Size: 148 4350 1250 484 5320
Fragments arranged by size:
5320 4350 1250 484 148
PfoI T'CCnGG_A
Cuts at: 911 1115 3178 3311 3512 3653 4288 4613 5060
Size: 204 2063 133 201 141 635 325 447
Cuts at: 5060 5192 5351 6485 6908 6923 7645 8108 911
Size: 132 159 1134 423 15 722 463 4355
Fragments arranged by size:
4355 2063 1134 722 635 463 447 423
325 204 201 159 141 133 132 15
PmeI GTTT'AAAC
Cuts at: 8551 8551
Size: 11552
PmlI CAC'GTG
Cuts at: 2705 3435 3575 6953 7016 2705
Size: 730 140 3378 63 7241
Fragments arranged by size:
7241 3378 730 140 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7121 7153 10691 10723 7121
Size: 32 3538 32 7950
Fragments arranged by size:
7950 3538 32 32
PpuMI rG'GwC_Cy
Cuts at: 164 641 1037 1596 1788 2093 2816 6054 6528
Size: 477 396 559 192 305 723 3238 474
Cuts at: 6528 6564 6921 7767 164
Size: 36 357 846 3949
Fragments arranged by size:
3949 3238 846 723 559 477 474 396
357 305 192 36
PshAI GACnn'nnGTC
Cuts at: 7535 7535
Size: 11552
PspOMI G'GGCC_C
Cuts at: 4357 4542 4573 4998 7876 4357
Size: 185 31 425 2878 8033
Fragments arranged by size:
8033 2878 425 185 31
PspXI vC'TCGA_Gb
Cuts at: 2464 2464
Size: 11552
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1918 1950 1918
Size: 32 11520
PstI C_TGCA'G
Cuts at: 280 1096 1246 1604 1796 2087 3925 4730 4796
Size: 816 150 358 192 291 1838 805 66
Cuts at: 4796 5108 5135 5167 5309 6088 6320 7195 8303
Size: 312 27 32 142 779 232 875 1108
Cuts at: 8303 8599 9284 280
Size: 296 685 2548
Fragments arranged by size:
2548 1838 1108 875 816 805 779 685
358 312 296 291 232 192 150 142
66 32 27
PvuI CG_AT'CG
Cuts at: 104 2717 104
Size: 2613 8939
PvuII CAG'CTG
Cuts at: 1097 3551 5542 6020 6848 7535 7761 7794 8977
Size: 2454 1991 478 828 687 226 33 1183
Cuts at: 8977 9337 1097
Size: 360 3312
Fragments arranged by size:
3312 2454 1991 1183 828 687 478 360
226 33
RsrII CG'GwC_CG
Cuts at: 9747 9747
Size: 11552
SacI G_AGCT'C
Cuts at: 3193 6784 8566 3193
Size: 3591 1782 6179
Fragments arranged by size:
6179 3591 1782
SacII CC_GC'GG
Cuts at: 1623 2000 7627 1623
Size: 377 5627 5548
Fragments arranged by size:
5627 5548 377
SalI G'TCGA_C
Cuts at: 8589 8589
Size: 11552
SanDI GG'GwC_CC
Cuts at: 164 2093 6921 164
Size: 1929 4828 4795
Fragments arranged by size:
4828 4795 1929
SapI GCTCTTCn'nnn_
Cuts at: 787 9575 9785 787
Size: 8788 210 2554
Fragments arranged by size:
8788 2554 210
SbfI CC_TGCA'GG
Cuts at: 7195 8599 7195
Size: 1404 10148
ScaI AGT'ACT
Cuts at: 4732 8158 11069 4732
Size: 3426 2911 5215
Fragments arranged by size:
5215 3426 2911
SexAI A'CCwGG_T
Cuts at: 1837 3938 4913 5096 1837
Size: 2101 975 183 8293
Fragments arranged by size:
8293 2101 975 183
SfcI C'TryA_G
Cuts at: 33 276 1092 1242 1600 1792 2083 3285 3921
Size: 243 816 150 358 192 291 1202 636
Cuts at: 3921 4726 4792 5104 5131 5163 5305 6084 6316
Size: 805 66 312 27 32 142 779 232
Cuts at: 6316 7191 8299 8595 9280 10273 10464 33
Size: 875 1108 296 685 993 191 1121
Fragments arranged by size:
1202 1121 1108 993 875 816 805 779
685 636 358 312 296 291 243 232
192 191 150 142 66 32 27
SfoI GGC'GCC
Cuts at: 1980 4907 8984 9232 1980
Size: 2927 4077 248 4300
Fragments arranged by size:
4300 4077 2927 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11552
SmaI CCC'GGG
Cuts at: 1354 1741 2543 2766 4547 5775 7881 8574 1354
Size: 387 802 223 1781 1228 2106 693 4332
Fragments arranged by size:
4332 2106 1781 1228 802 693 387 223
SmlI C'TyrA_G
Cuts at: 1199 2464 4099 4372 8707 9622 10114 10376 1199
Size: 1265 1635 273 4335 915 492 262 2375
Fragments arranged by size:
4335 2375 1635 1265 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11552
SphI G_CATG'C
Cuts at: 7857 8605 9105 7857
Size: 748 500 10304
Fragments arranged by size:
10304 748 500
StuI AGG'CCT
Cuts at: 505 1127 1888 2152 3833 4000 4417 5880 6865
Size: 622 761 264 1681 167 417 1463 985
Cuts at: 6865 7849 505
Size: 984 4208
Fragments arranged by size:
4208 1681 1463 985 984 761 622 417
264 167
StyI C'CwwG_G
Cuts at: 1502 2216 2417 4589 5249 5338 5426 5684 5693
Size: 714 201 2172 660 89 88 258 9
Cuts at: 5693 6270 7326 8510 8686 9040 1502
Size: 577 1056 1184 176 354 4014
Fragments arranged by size:
4014 2172 1184 1056 714 660 577 354
258 201 176 89 88 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 979 6199 7529 9135 9268 9727 9936 979
Size: 5220 1330 1606 133 459 209 2595
Fragments arranged by size:
5220 2595 1606 1330 459 209 133
TatI w'GTAC_w
Cuts at: 257 4730 8156 8384 11067 257
Size: 4473 3426 228 2683 742
Fragments arranged by size:
4473 3426 2683 742 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 156 188 1134 1166 1360 1392 2615 2647 2656
Size: 32 946 32 194 32 1223 32 9
Cuts at: 2656 2688 3067 3099 3735 3767 3821 3853 4063
Size: 32 379 32 636 32 54 32 210
Cuts at: 4063 4095 4920 4952 6276 6308 6481 6513 6610
Size: 32 825 32 1324 32 173 32 97
Cuts at: 6610 6642 7368 7400 8260 8292 11069 11101 156
Size: 32 726 32 860 32 2777 32 607
Fragments arranged by size:
2777 1324 1223 946 860 825 726 636
607 379 210 194 173 97 54 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 232 2359 3940 9349 232
Size: 2127 1581 5409 2435
Fragments arranged by size:
5409 2435 2127 1581
XbaI T'CTAG_A
Cuts at: 465 8583 465
Size: 8118 3434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2424 2718 3879 4842 5691 6745 7422 9047 2424
Size: 294 1161 963 849 1054 677 1625 4929
Fragments arranged by size:
4929 1625 1161 1054 963 849 677 294
XhoI C'TCGA_G
Cuts at: 1199 2464 1199
Size: 1265 10287
XmaI C'CCGG_G
Cuts at: 1352 1739 2541 2764 4545 5773 7879 8572 1352
Size: 387 802 223 1781 1228 2106 693 4332
Fragments arranged by size:
4332 2106 1781 1228 802 693 387 223
XmnI GAAnn'nnTTC
Cuts at: 5242 5242
Size: 11552
ZraI GAC'GTC
Cuts at: 1518 1871 3306 5385 5514 5781 1518
Size: 353 1435 2079 129 267 7289
Fragments arranged by size:
7289 2079 1435 353 267 129
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BmtI BsiWI BssHII BstZ17I FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PsiI SfiI SgrAI SnaBI SrfI SspI SwaI