Restriction map for pF1KB6008
 (Linear) (Six-base) MAPSORT of: KIBB6008.seq  Check: 1967  from: 1  to: 8439



KIBB6008.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1114    3142    3271    3459    7084    7279    7363    7812 
   Size:       1114    2028     129     188    3625     195      84     449

Cuts at:   7812    7876    8149    8337    8439 
   Size:         64     273     188     102

  Fragments arranged by size: 

                3625    2028    1114     449     273     195     188     188
                 129     102      84      64
 
AatII G_ACGT'C

Cuts at:      0    1412    1765    3200    5279    5408    5675    8439 
   Size:       1412     353    1435    2079     129     267    2764

  Fragments arranged by size: 

                2764    2079    1435    1412     353     267     129
 
AccI GT'mk_AC

Cuts at:      0    4811    6623    8439 
   Size:       4811    1812    1816

  Fragments arranged by size: 

                4811    1816    1812
 
Acc65I G'GTAC_C

Cuts at:      0     298    4748    8439 
   Size:        298    4450    3691

  Fragments arranged by size: 

                4450    3691     298
 
AclI AA'CG_TT

Cuts at:      0    6122    8439 
   Size:       6122    2317
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     703    1200    1393    2369    2971    3599    3907 
   Size:        527     176     497     193     976     602     628     308

Cuts at:   3907    4089    4145    4231    4406    4638    5330    5755    6770 
   Size:        182      56      86     175     232     692     425    1015

Cuts at:   6770    8002    8243    8439 
   Size:       1232     241     196

  Fragments arranged by size: 

                1232    1015     976     692     628     602     527     497
                 425     308     241     232     196     193     182     176
                 175      86      56
 
AfeI AGC'GCT

Cuts at:      0    1066    8439 
   Size:       1066    7373
 
AflIII A'CryG_T

Cuts at:      0    1832    3326    3466    6907    8152    8439 
   Size:       1832    1494     140    3441    1245     287

  Fragments arranged by size: 

                3441    1832    1494    1245     287     140
 
AhdI GACnn_n'nnGTC

Cuts at:      0    6245    7530    8439 
   Size:       6245    1285     909

  Fragments arranged by size: 

                6245    1285     909
 
AleI CACnn'nnGTG

Cuts at:      0     694    3329    5185    8439 
   Size:        694    2635    1856    3254

  Fragments arranged by size: 

                3254    2635    1856     694
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     207    4159    5070    6969    8439 
   Size:        207    3952     911    1899    1470

  Fragments arranged by size: 

                3952    1899    1470     911     207
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4483    4515    7013    7045    8439 
   Size:       4483      32    2498      32    1394

  Fragments arranged by size: 

                4483    2498    1394      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     681     932     942    1008    1236    1683    4209    4914 
   Size:        681     251      10      66     228     447    2526     705

Cuts at:   4914    5067    5434    5949    6801    8439 
   Size:        153     367     515     852    1638

  Fragments arranged by size: 

                2526    1638     852     705     681     515     447     367
                 251     228     153      66      10
 
ApaI G_GGCC'C

Cuts at:      0    4253    4438    4469    4894    7772    8439 
   Size:       4253     185      31     425    2878     667

  Fragments arranged by size: 

                4253    2878     667     425     185      31
 
ApaLI G'TGCA_C

Cuts at:      0    1317    3446    5847    7350    7797    8439 
   Size:       1317    2129    2401    1503     447     642

  Fragments arranged by size: 

                2401    2129    1503    1317     642     447
 
ApoI r'AATT_y

Cuts at:      0     268    2991    5892    8129    8439 
   Size:        268    2723    2901    2237     310

  Fragments arranged by size: 

                2901    2723    2237     310     268
 
AvaI C'yCGr_G

Cuts at:      0     727    1091    1244    1631    2356    2433    2656    3229 
   Size:        727     364     153     387     725      77     223     573

Cuts at:   3229    4437    4973    5665    6961    7674    7771    8439 
   Size:       1208     536     692    1296     713      97     668

  Fragments arranged by size: 

                1296    1208     727     725     713     692     668     573
                 536     387     364     223     153      97      77
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     156     189    8439 
   Size:        156      33    8250

  Fragments arranged by size: 

                8250     156      33
 
BamHI G'GATC_C

Cuts at:      0    2717    8439 
   Size:       2717    5722
 
BanI G'GyrC_C

Cuts at:      0     298    1078    1870    3732    4748    4797    4875    5359 
   Size:        298     780     792    1862    1016      49      78     484

Cuts at:   5359    5971    6130    7478    8137    8439 
   Size:        612     159    1348     659     302

  Fragments arranged by size: 

                1862    1348    1016     792     780     659     612     484
                 302     298     159      78      49
 
BanII G_rGCy'C

Cuts at:      0     576    1631    2078    2357    2642    3085    3760    3992 
   Size:        576    1055     447     279     285     443     675     232

Cuts at:   3992    4169    4253    4438    4445    4469    4894    6676    7772 
   Size:        177      84     185       7      24     425    1782    1096

Cuts at:   7772    8342    8439 
   Size:        570      97

  Fragments arranged by size: 

                1782    1096    1055     675     576     570     447     443
                 425     285     279     232     185     177      97      84
                  24       7
 
BbeI G_GCGC'C

Cuts at:      0    1874    4801    8439 
   Size:       1874    2927    3638

  Fragments arranged by size: 

                3638    2927    1874
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     760    1561    2162    3053    3192    3587    3828    5570 
   Size:        760     801     601     891     139     395     241    1742

Cuts at:   5570    7159    7526    7783    7867    8439 
   Size:       1589     367     257      84     572

  Fragments arranged by size: 

                1742    1589     891     801     760     601     572     395
                 367     257     241     139      84
 
BbvCI CC'TCA_GC

Cuts at:      0    1474    2257    5767    6292    6850    7701    8108    8439 
   Size:       1474     783    3510     525     558     851     407     331

  Fragments arranged by size: 

                3510    1474     851     783     558     525     407     331
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127    3292    3326    8164    8198    8439 
   Size:         93      34    3165      34    4838      34     241

  Fragments arranged by size: 

                4838    3165     241      93      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     157    8439 
   Size:        157    8282
 
BfrBI ATG'CAT

Cuts at:      0    5355    6141    8439 
   Size:       5355     786    2298

  Fragments arranged by size: 

                5355    2298     786
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1868    1961    2054    2435    5380    5528    7676    8439 
   Size:       1868      93      93     381    2945     148    2148     763

  Fragments arranged by size: 

                2945    2148    1868     763     381     148      93      93
            
 
BglII A'GATC_T

Cuts at:      0    2141    6836    8439 
   Size:       2141    4695    1603

  Fragments arranged by size: 

                4695    2141    1603
 
BlpI GC'TnA_GC

Cuts at:      0    4318    5263    6214    7687    8439 
   Size:       4318     945     951    1473     752

  Fragments arranged by size: 

                4318    1473     951     945     752
 
Bme1580I G_kGCm'C

Cuts at:      0     828     886    1321    2108    2122    3336    3379    3450 
   Size:        828      58     435     787      14    1214      43      71

Cuts at:   3450    4253    4438    4469    4880    4894    5782    5851    6033 
   Size:        803     185      31     411      14     888      69     182

Cuts at:   6033    7354    7772    7801    8439 
   Size:       1321     418      29     638

  Fragments arranged by size: 

                1321    1214     888     828     803     787     638     435
                 418     411     185     182      71      69      58      43
                  31      29      14      14
 
BmgBI CAC'GTC

Cuts at:      0    1833    4608    5073    5226    6365    8439 
   Size:       1833    2775     465     153    1139    2074

  Fragments arranged by size: 

                2775    2074    1833    1139     465     153
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     871    1512    2109    3641    4742    6979    7000    7152 
   Size:        871     641     597    1532    1101    2237      21     152

Cuts at:   7152    7392    7524    7551    8439 
   Size:        240     132      27     888

  Fragments arranged by size: 

                2237    1532    1101     888     871     641     597     240
                 152     132      27      21
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     265    3801    8439 
   Size:        265    3536    4638

  Fragments arranged by size: 

                4638    3536     265
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802     826    2071    2410    2534    3378    3518    3840 
   Size:        802      24    1245     339     124     844     140     322

Cuts at:   3840    4357    4880    4936    5082    5536    5971    6005    6400 
   Size:        517     523      56     146     454     435      34     395

Cuts at:   6400    6449    6597    6698    7015    7675    7870    7984    8439 
   Size:         49     148     101     317     660     195     114     455

  Fragments arranged by size: 

                1245     844     802     660     523     517     455     454
                 435     395     339     322     317     195     148     146
                 140     124     114     101      56      49      34      24
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1474    2257    2932    3479    3656    5767    6292    6850 
   Size:       1474     783     675     547     177    2111     525     558

Cuts at:   6850    7701    8108    8439 
   Size:        851     407     331

  Fragments arranged by size: 

                2111    1474     851     783     675     558     547     525
                 407     331     177
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3976    4249    8439 
   Size:       3976     273    4190

  Fragments arranged by size: 

                4190    3976     273
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    5806    6090    8439 
   Size:       5806     284    2349

  Fragments arranged by size: 

                5806    2349     284
 
BsaAI yAC'GTr

Cuts at:      0    2597    3327    3467    6099    6845    6908    8439 
   Size:       2597     730     140    2632     746      63    1531

  Fragments arranged by size: 

                2632    2597    1531     746     730     140      63
 
BsaHI Gr'CG_yC

Cuts at:      0    1409    1762    1871    3197    4798    5276    5405    5672 
   Size:       1409     353     109    1326    1601     478     129     267

Cuts at:   5672    8439 
   Size:       2767

  Fragments arranged by size: 

                2767    1601    1409    1326     478     353     267     129
                 109
 
BsaWI w'CCGG_w

Cuts at:      0     388    1057    2558    3998    4822    5011    8439 
   Size:        388     669    1501    1440     824     189    3428

  Fragments arranged by size: 

                3428    1501    1440     824     669     388     189
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1252    1282    1822    1852    2507    2537    2653    2683 
   Size:       1252      30     540      30     655      30     116      30

Cuts at:   2683    2959    2989    3250    3280    3400    3430    3713    3743 
   Size:        276      30     261      30     120      30     283      30

Cuts at:   3743    3955    3985    6168    6198    6373    6403    6502    6532 
   Size:        212      30    2183      30     175      30      99      30

Cuts at:   6532    6658    6676    6688    6706    7013    7043    8152    8182 
   Size:        126      18      12      18     307      30    1109      30

Cuts at:   8182    8296    8326    8439 
   Size:        114      30     113

  Fragments arranged by size: 

                2183    1252    1109     655     540     307     283     276
                 261     212     175     126     120     116     114     113
                  99      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     602     627    1756    2807    3406    3843    4385    4579 
   Size:        602      25    1129    1051     599     437     542     194

Cuts at:   4579    5085    6397    6415    7108    7348    7627    7976    8057 
   Size:        506    1312      18     693     240     279     349      81

Cuts at:   8057    8173    8439 
   Size:        116     266

  Fragments arranged by size: 

                1312    1129    1051     693     602     599     542     506
                 437     349     279     266     240     194     116      81
                  25      18
 
BseYI C'CCAG_C

Cuts at:      0     370     616     772    2895    3335    5106    5299    5501 
   Size:        370     246     156    2123     440    1771     193     202

Cuts at:   5501    5523    7649    8439 
   Size:         22    2126     790

  Fragments arranged by size: 

                2126    2123    1771     790     440     370     246     202
                 193     156      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     891     928    1336    1648    2556    2701    3520 
   Size:         86     805      37     408     312     908     145     819

Cuts at:   3520    4927    5030    6031    6367    6744    6825    6855    7080 
   Size:       1407     103    1001     336     377      81      30     225

Cuts at:   7080    7341    7840    7875    8320    8341    8346    8439 
   Size:        261     499      35     445      21       5      93

  Fragments arranged by size: 

                1407    1001     908     819     805     499     445     408
                 377     336     312     261     225     145     103      93
                  86      81      37      35      30      21       5
 
BsiEI CG_ry'CG

Cuts at:      0    1854    2609    4009    4119    7435    8439 
   Size:       1854     755    1400     110    3316    1004

  Fragments arranged by size: 

                3316    1854    1400    1004     755     110
 
BsiHKAI G_wGCw'C

Cuts at:      0     691    1036    1321    1840    2602    3085    3450    3662 
   Size:        691     345     285     519     762     483     365     212

Cuts at:   3662    5617    5851    6676    7354    7405    7801    8314    8439 
   Size:       1955     234     825     678      51     396     513     125

  Fragments arranged by size: 

                1955     825     762     691     678     519     513     483
                 396     365     345     285     234     212     125      51
            
 
BsmI GAATG_Cn'

Cuts at:      0    1053    2038    6451    6948    8439 
   Size:       1053     985    4413     497    1491

  Fragments arranged by size: 

                4413    1491    1053     985     497
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4957    5182    5412    7644    7987    8439 
   Size:       4957     225     230    2232     343     452

  Fragments arranged by size: 

                4957    2232     452     343     230     225
 
Bsp1286I G_dGCh'C

Cuts at:      0     576     691     828     886    1036    1321    1631    1840 
   Size:        576     115     137      58     150     285     310     209

Cuts at:   1840    2078    2108    2122    2357    2602    2642    3085    3336 
   Size:        238      30      14     235     245      40     443     251

Cuts at:   3336    3379    3450    3662    3760    3992    4169    4253    4438 
   Size:         43      71     212      98     232     177      84     185

Cuts at:   4438    4445    4469    4880    4894    5617    5782    5851    6033 
   Size:          7      24     411      14     723     165      69     182

Cuts at:   6033    6676    7354    7405    7772    7801    8314    8342    8439 
   Size:        643     678      51     367      29     513      28      97

  Fragments arranged by size: 

                 723     678     643     576     513     443     411     367
                 310     285     251     245     238     235     232     212
                 209     185     182     177     165     150     137     115
                  98      97      84      71      69      58      51      43
                  40      30      29      28      24      14      14       7
            
 
BspEI T'CCGG_A

Cuts at:      0    1057    5011    8439 
   Size:       1057    3954    3428

  Fragments arranged by size: 

                3954    3428    1057
 
BspHI T'CATG_A

Cuts at:      0    3149    7973    8439 
   Size:       3149    4824     466

  Fragments arranged by size: 

                4824    3149     466
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     481     940    1114    1244    1499    1567    1691 
   Size:         74     407     459     174     130     255      68     124

Cuts at:   1691    1820    1982    2011    2348    3142    3271    3459    3574 
   Size:        129     162      29     337     794     129     188     115

Cuts at:   3574    5842    7007    7084    7279    7363    7812    7813    7876 
   Size:       2268    1165      77     195      84     449       1      63

Cuts at:   7876    8149    8337    8439 
   Size:        273     188     102

  Fragments arranged by size: 

                2268    1165     794     459     449     407     337     273
                 255     195     188     188     174     162     130     129
                 129     124     115     102      84      77      74      68
                  63      29       1
 
BsrBI CCG'CTC

Cuts at:      0    1325    2009    3915    8439 
   Size:       1325     684    1906    4524

  Fragments arranged by size: 

                4524    1906    1325     684
 
BsrDI GCAATG_nn'

Cuts at:      0    1146    2043    3308    4025    8439 
   Size:       1146     897    1265     717    4414

  Fragments arranged by size: 

                4414    1265    1146     897     717
 
BsrFI r'CCGG_y

Cuts at:      0    2876    3608    8439 
   Size:       2876     732    4831

  Fragments arranged by size: 

                4831    2876     732
 
BsrGI T'GTAC_A

Cuts at:      0     149    8439 
   Size:        149    8290
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     626    1106    1390    4692    5437    6767    7137    7805 
   Size:        626     480     284    3302     745    1330     370     668

Cuts at:   7805    7877    8439 
   Size:         72     562

  Fragments arranged by size: 

                3302    1330     745     668     626     562     480     370
                 284      72
 
BstEII G'GTnAC_C

Cuts at:      0    1432    2772    3351    4635    4783    4818    5580    8439 
   Size:       1432    1340     579    1284     148      35     762    2859

  Fragments arranged by size: 

                2859    1432    1340    1284     762     579     148      35
            
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2784    4488    4887    5719    8349    8439 
   Size:       2784    1704     399     832    2630      90

  Fragments arranged by size: 

                2784    2630    1704     832     399      90
 
BstYI r'GATC_y

Cuts at:      0    1130    1223    2141    2717    4271    4700    5027    5510 
   Size:       1130      93     918     576    1554     429     327     483

Cuts at:   5510    6257    6836    8439 
   Size:        747     579    1603

  Fragments arranged by size: 

                1603    1554    1130     918     747     579     576     483
                 429     327      93
 
Bsu36I CC'TnA_GG

Cuts at:      0     995    3621    8439 
   Size:        995    2626    4818

  Fragments arranged by size: 

                4818    2626     995
 
BtgI C'CryG_G

Cuts at:      0      92    1394    1512    1889    2309    2912    2942    3950 
   Size:         92    1302     118     377     420     603      30    1008

Cuts at:   3950    4481    4640    5230    5576    6890    7516    8402    8439 
   Size:        531     159     590     346    1314     626     886      37

  Fragments arranged by size: 

                1314    1302    1008     886     626     603     590     531
                 420     377     346     159     118      92      37      30
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     984    2260    4122    7358    8439 
   Size:        984    1276    1862    3236    1081

  Fragments arranged by size: 

                3236    1862    1276    1081     984
 
BtsI GCAGTG_nn'

Cuts at:      0     588     676     913    1984    3094    3508    3525    3660 
   Size:        588      88     237    1071    1110     414      17     135

Cuts at:   3660    4329    6394    6411    8180    8358    8439 
   Size:        669    2065      17    1769     178      81

  Fragments arranged by size: 

                2065    1769    1110    1071     669     588     414     237
                 178     135      88      81      17      17
 
ClaI AT'CG_AT

Cuts at:      0     419    7790    8439 
   Size:        419    7371     649

  Fragments arranged by size: 

                7371     649     419
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    5366    5401    7233    7268    8439 
   Size:       5366      35    1832      35    1171

  Fragments arranged by size: 

                5366    1832    1171      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1040    2183    3374    5703    5874    6767    8439 
   Size:       1040    1143    1191    2329     171     893    1672

  Fragments arranged by size: 

                2329    1672    1191    1143    1040     893     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     322    8439 
   Size:        322    8117
 
EaeI y'GGCC_r

Cuts at:      0     410    1401    1509    1851    1877    2874    3365    3606 
   Size:        410     991     108     342      26     997     491     241

Cuts at:   3606    3947    4154    5721    6352    6893    8439 
   Size:        341     207    1567     631     541    1546

  Fragments arranged by size: 

                1567    1546     997     991     631     541     491     410
                 342     341     241     207     108      26
 
EagI C'GGCC_G

Cuts at:      0    1851    8439 
   Size:       1851    6588
 
EarI CTCTTCn'nnn_

Cuts at:      0     679    2824    5125    5653    5731    5804    6746    7537 
   Size:        679    2145    2301     528      78      73     942     791

Cuts at:   7537    8056    8439 
   Size:        519     383

  Fragments arranged by size: 

                2301    2145     942     791     679     528     519     383
                  78      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1639    4264    6873    6925    8439 
   Size:       1639    2625    2609      52    1514

  Fragments arranged by size: 

                2625    2609    1639    1514      52
 
EcoICRI GAG'CTC

Cuts at:      0    3083    6674    8439 
   Size:       3083    3591    1765

  Fragments arranged by size: 

                3591    3083    1765
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     703     802     826    1200    1393    2071    2369 
   Size:        527     176      99      24     374     193     678     298

Cuts at:   2369    2410    2534    2971    3378    3518    3599    3840    3907 
   Size:         41     124     437     407     140      81     241      67

Cuts at:   3907    4089    4145    4231    4357    4406    4638    4880    4936 
   Size:        182      56      86     126      49     232     242      56

Cuts at:   4936    5082    5330    5536    5755    5971    6005    6400    6449 
   Size:        146     248     206     219     216      34     395      49

Cuts at:   6449    6597    6698    6770    7015    7675    7870    7984    8002 
   Size:        148     101      72     245     660     195     114      18

Cuts at:   8002    8243    8439 
   Size:        241     196

  Fragments arranged by size: 

                 678     660     527     437     407     395     374     298
                 248     245     242     241     241     232     219     216
                 206     196     195     193     182     176     148     146
                 140     126     124     114     101      99      86      81
                  72      67      56      56      49      49      41      34
                  24      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2785    4951    8439 
   Size:       2785    2166    3488

  Fragments arranged by size: 

                3488    2785    2166
 
EcoO109I rG'GnC_Cy

Cuts at:      0      56     533     657     929    1488    1680    1985    2708 
   Size:         56     477     124     272     559     192     305     723

Cuts at:   2708    4206    4250    4293    4465    4891    5170    5296    5946 
   Size:       1498      44      43     172     426     279     126     650

Cuts at:   5946    6420    6456    6813    7659    8439 
   Size:        474      36     357     846     780

  Fragments arranged by size: 

                1498     846     780     723     650     559     477     474
                 426     357     305     279     272     192     172     126
                 124      56      44      43      36
 
EcoRV GAT'ATC

Cuts at:      0    8238    8439 
   Size:       8238     201
 
FspI TGC'GCA

Cuts at:      0    3922    8439 
   Size:       3922    4517
 
HaeII r_GCGC'y

Cuts at:      0    1068    1874    3435    4801    8439 
   Size:       1068     806    1561    1366    3638

  Fragments arranged by size: 

                3638    1561    1366    1068     806
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1822    1854    2149    2181    3250    3282    3838    3870 
   Size:       1822      32     295      32    1069      32     556      32

Cuts at:   3870    4651    4683    5080    5112    6231    6263    6676    6708 
   Size:        781      32     397      32    1119      32     413      32

Cuts at:   6708    7516    7548    7955    7987    8296    8328    8439 
   Size:        808      32     407      32     309      32     111

  Fragments arranged by size: 

                1822    1119    1069     808     781     556     413     407
                 397     309     295     111      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     129    3573    3690    5751    6120    6573    6867    7197 
   Size:        129    3444     117    2061     369     453     294     330

Cuts at:   7197    8439 
   Size:       1242

  Fragments arranged by size: 

                3444    2061    1242     453     369     330     294     129
                 117
 
HindIII A'AGCT_T

Cuts at:      0    2235    8439 
   Size:       2235    6204
 
KasI G'GCGC_C

Cuts at:      0    1870    4797    8439 
   Size:       1870    2927    3642

  Fragments arranged by size: 

                3642    2927    1870
 
KpnI G_GTAC'C

Cuts at:      0     302    4752    8439 
   Size:        302    4450    3687

  Fragments arranged by size: 

                4450    3687     302
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    5474    5561    6228    6532    6710    8350    8439 
   Size:       5474      87     667     304     178    1640      89

  Fragments arranged by size: 

                5474    1640     667     304     178      89      87
 
MscI TGG'CCA

Cuts at:      0     412    1403    1879    4156    5723    8439 
   Size:        412     991     476    2277    1567    2716

  Fragments arranged by size: 

                2716    2277    1567     991     476     412
 
MslI CAynn'nnrTG

Cuts at:      0     694     950    2401    2585    2782    2941    3329    3680 
   Size:        694     256    1451     184     197     159     388     351

Cuts at:   3680    4870    4885    5185    5224    5969    6016    6061    6659 
   Size:       1190      15     300      39     745      47      45     598

Cuts at:   6659    7363    8347    8439 
   Size:        704     984      92

  Fragments arranged by size: 

                1451    1190     984     745     704     694     598     388
                 351     300     256     197     184     159      92      47
                  45      39      15
 
MspA1I CmG'CkG

Cuts at:      0     428     707     989    1514    1803    1891    1940    2332 
   Size:        428     279     282     525     289      88      49     392

Cuts at:   2332    3443    4578    4689    5286    5434    5828    5912    6351 
   Size:       1111    1135     111     597     148     394      84     439

Cuts at:   6351    6740    6854    7427    7518    7653    7686    7811    8439 
   Size:        389     114     573      91     135      33     125     628

  Fragments arranged by size: 

                1135    1111     628     597     573     525     439     428
                 394     392     389     289     282     279     148     135
                 125     114     111      91      88      84      49      33
            
 
NaeI GCC'GGC

Cuts at:      0    3610    8439 
   Size:       3610    4829
 
NarI GG'CG_CC

Cuts at:      0    1871    4798    8439 
   Size:       1871    2927    3641

  Fragments arranged by size: 

                3641    2927    1871
 
NcoI C'CATG_G

Cuts at:      0    1394    2309    4481    5230    5576    8402    8439 
   Size:       1394     915    2172     749     346    2826      37

  Fragments arranged by size: 

                2826    2172    1394     915     749     346      37
 
NgoMIV G'CCGG_C

Cuts at:      0    3608    8439 
   Size:       3608    4831
 
NsiI A_TGCA'T

Cuts at:      0    5357    6143    8439 
   Size:       5357     786    2296

  Fragments arranged by size: 

                5357    2296     786
 
NspI r_CATG'y

Cuts at:      0     150    7749    8156    8439 
   Size:        150    7599     407     283

  Fragments arranged by size: 

                7599     407     283     150
 
PasI CC'CwG_GG

Cuts at:      0    2071    2704    4461    7884    8439 
   Size:       2071     633    1757    3423     555

  Fragments arranged by size: 

                3423    2071    1757     633     555
 
PciI A'CATG_T

Cuts at:      0    8152    8439 
   Size:       8152     287
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     569     717    5067    6317    6801    8439 
   Size:        569     148    4350    1250     484    1638

  Fragments arranged by size: 

                4350    1638    1250     569     484     148
 
PfoI T'CCnGG_A

Cuts at:      0     803    1007    3070    3203    3404    3545    4180    4505 
   Size:        803     204    2063     133     201     141     635     325

Cuts at:   4505    4952    5084    5243    6377    6800    6815    7537    8000 
   Size:        447     132     159    1134     423      15     722     463

Cuts at:   8000    8439 
   Size:        439

  Fragments arranged by size: 

                2063    1134     803     722     635     463     447     439
                 423     325     204     201     159     141     133     132
                  15
 
PmlI CAC'GTG

Cuts at:      0    2597    3327    3467    6845    6908    8439 
   Size:       2597     730     140    3378      63    1531

  Fragments arranged by size: 

                3378    2597    1531     730     140      63
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    7013    7045    8439 
   Size:       7013      32    1394

  Fragments arranged by size: 

                7013    1394      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      56     533     929    1488    1680    1985    2708    5946 
   Size:         56     477     396     559     192     305     723    3238

Cuts at:   5946    6420    6456    6813    7659    8439 
   Size:        474      36     357     846     780

  Fragments arranged by size: 

                3238     846     780     723     559     477     474     396
                 357     305     192      56      36
 
PshAI GACnn'nnGTC

Cuts at:      0    7427    8439 
   Size:       7427    1012
 
PspOMI G'GGCC_C

Cuts at:      0    4249    4434    4465    4890    7768    8439 
   Size:       4249     185      31     425    2878     671

  Fragments arranged by size: 

                4249    2878     671     425     185      31
 
PspXI vC'TCGA_Gb

Cuts at:      0    2356    8439 
   Size:       2356    6083
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1810    1842    8439 
   Size:       1810      32    6597

  Fragments arranged by size: 

                6597    1810      32
 
PstI C_TGCA'G

Cuts at:      0     172     988    1138    1496    1688    1979    3817    4622 
   Size:        172     816     150     358     192     291    1838     805

Cuts at:   4622    4688    5000    5027    5059    5201    5980    6212    7087 
   Size:         66     312      27      32     142     779     232     875

Cuts at:   7087    8195    8439 
   Size:       1108     244

  Fragments arranged by size: 

                1838    1108     875     816     805     779     358     312
                 291     244     232     192     172     150     142      66
                  32      27
 
PvuI CG_AT'CG

Cuts at:      0    2609    8439 
   Size:       2609    5830
 
PvuII CAG'CTG

Cuts at:      0     989    3443    5434    5912    6740    7427    7653    7686 
   Size:        989    2454    1991     478     828     687     226      33

Cuts at:   7686    8439 
   Size:        753

  Fragments arranged by size: 

                2454    1991     989     828     753     687     478     226
                  33
 
SacI G_AGCT'C

Cuts at:      0    3085    6676    8439 
   Size:       3085    3591    1763

  Fragments arranged by size: 

                3591    3085    1763
 
SacII CC_GC'GG

Cuts at:      0    1515    1892    7519    8439 
   Size:       1515     377    5627     920

  Fragments arranged by size: 

                5627    1515     920     377
 
SanDI GG'GwC_CC

Cuts at:      0      56    1985    6813    8439 
   Size:         56    1929    4828    1626

  Fragments arranged by size: 

                4828    1929    1626      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0     679    8439 
   Size:        679    7760
 
SbfI CC_TGCA'GG

Cuts at:      0    7087    8439 
   Size:       7087    1352
 
ScaI AGT'ACT

Cuts at:      0    4624    8050    8439 
   Size:       4624    3426     389

  Fragments arranged by size: 

                4624    3426     389
 
SexAI A'CCwGG_T

Cuts at:      0    1729    3830    4805    4988    8439 
   Size:       1729    2101     975     183    3451

  Fragments arranged by size: 

                3451    2101    1729     975     183
 
SfcI C'TryA_G

Cuts at:      0     168     984    1134    1492    1684    1975    3177    3813 
   Size:        168     816     150     358     192     291    1202     636

Cuts at:   3813    4618    4684    4996    5023    5055    5197    5976    6208 
   Size:        805      66     312      27      32     142     779     232

Cuts at:   6208    7083    8191    8439 
   Size:        875    1108     248

  Fragments arranged by size: 

                1202    1108     875     816     805     779     636     358
                 312     291     248     232     192     168     150     142
                  66      32      27
 
SfoI GGC'GCC

Cuts at:      0    1872    4799    8439 
   Size:       1872    2927    3640

  Fragments arranged by size: 

                3640    2927    1872
 
SmaI CCC'GGG

Cuts at:      0    1246    1633    2435    2658    4439    5667    7773    8439 
   Size:       1246     387     802     223    1781    1228    2106     666

  Fragments arranged by size: 

                2106    1781    1246    1228     802     666     387     223
            
 
SmlI C'TyrA_G

Cuts at:      0    1091    2356    3991    4264    8439 
   Size:       1091    1265    1635     273    4175

  Fragments arranged by size: 

                4175    1635    1265    1091     273
 
SphI G_CATG'C

Cuts at:      0    7749    8439 
   Size:       7749     690
 
StuI AGG'CCT

Cuts at:      0     397    1019    1780    2044    3725    3892    4309    5772 
   Size:        397     622     761     264    1681     167     417    1463

Cuts at:   5772    6757    7741    8439 
   Size:        985     984     698

  Fragments arranged by size: 

                1681    1463     985     984     761     698     622     417
                 397     264     167
 
StyI C'CwwG_G

Cuts at:      0    1394    2108    2309    4481    5141    5230    5318    5576 
   Size:       1394     714     201    2172     660      89      88     258

Cuts at:   5576    5585    6162    7218    8402    8439 
   Size:          9     577    1056    1184      37

  Fragments arranged by size: 

                2172    1394    1184    1056     714     660     577     258
                 201      89      88      37       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     871    6091    7421    8439 
   Size:        871    5220    1330    1018

  Fragments arranged by size: 

                5220    1330    1018     871
 
TatI w'GTAC_w

Cuts at:      0     149    4622    8048    8276    8439 
   Size:        149    4473    3426     228     163

  Fragments arranged by size: 

                4473    3426     228     163     149
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      48      80    1026    1058    1252    1284    2507    2539 
   Size:         48      32     946      32     194      32    1223      32

Cuts at:   2539    2548    2580    2959    2991    3627    3659    3713    3745 
   Size:          9      32     379      32     636      32      54      32

Cuts at:   3745    3955    3987    4812    4844    6168    6200    6373    6405 
   Size:        210      32     825      32    1324      32     173      32

Cuts at:   6405    6502    6534    7260    7292    8152    8184    8439 
   Size:         97      32     726      32     860      32     255

  Fragments arranged by size: 

                1324    1223     946     860     825     726     636     379
                 255     210     194     173      97      54      48      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32       9
 
Tth111I GACn'n_nGTC

Cuts at:      0     124    2251    3832    8439 
   Size:        124    2127    1581    4607

  Fragments arranged by size: 

                4607    2127    1581     124
 
XbaI T'CTAG_A

Cuts at:      0     357    8439 
   Size:        357    8082
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2316    2610    3771    4734    5583    6637    7314    8439 
   Size:       2316     294    1161     963     849    1054     677    1125

  Fragments arranged by size: 

                2316    1161    1125    1054     963     849     677     294
            
 
XhoI C'TCGA_G

Cuts at:      0    1091    2356    8439 
   Size:       1091    1265    6083

  Fragments arranged by size: 

                6083    1265    1091
 
XmaI C'CCGG_G

Cuts at:      0    1244    1631    2433    2656    4437    5665    7771    8439 
   Size:       1244     387     802     223    1781    1228    2106     668

  Fragments arranged by size: 

                2106    1781    1244    1228     802     668     387     223
            
 
XmnI GAAnn'nnTTC

Cuts at:      0    5134    8439 
   Size:       5134    3305
 
ZraI GAC'GTC

Cuts at:      0    1410    1763    3198    5277    5406    5673    8439 
   Size:       1410     353    1435    2079     129     267    2766

  Fragments arranged by size: 

                2766    2079    1435    1410     353     267     129
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII     SacI    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AscI     AseI    AsiSI    AvrII     BclI     BmtI
    BsaBI    BsiWI   BssHII    BssSI    BstBI  BstZ17I     DraI    EcoRI
     FalI     FseI    FspAI     HpaI     MfeI     MluI     NdeI     NheI
     NotI     NruI     PacI     PmeI     PsiI    RsrII     SalI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
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