Restriction map for
pF1KB6004
(Circular) (Six-base) MAPSORT of: pF1KB6004.seq Check: 9230 from: 1 to: 9047
pF1KB6004 9047 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1470 6085 1470
Size: 4615 4432
Acc65I G'GTAC_C
Cuts at: 6063 6063
Size: 9047
AclI AA'CG_TT
Cuts at: 8887 8887
Size: 9047
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 3154 3178 3235 3644 3863 3917 5135 5393
Size: 959 24 57 409 219 54 1218 258
Cuts at: 5393 5810 5834 6044 6872 7304 8051 8319 2195
Size: 417 24 210 828 432 747 268 2923
Fragments arranged by size:
2923 1218 959 828 747 432 417 409
268 258 219 210 57 54 24 24
AfeI AGC'GCT
Cuts at: 1980 1980
Size: 9047
AflIII A'CryG_T
Cuts at: 1194 1649 3571 5956 6399 7503 8480 1194
Size: 455 1922 2385 443 1104 977 1761
Fragments arranged by size:
2385 1922 1761 1104 977 455 443
AgeI A'CCGG_T
Cuts at: 7435 7435
Size: 9047
AhdI GACnn_n'nnGTC
Cuts at: 1015 1076 2052 1015
Size: 61 976 8010
Fragments arranged by size:
8010 976 61
AleI CACnn'nnGTG
Cuts at: 1386 1386
Size: 9047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5359 5359
Size: 9047
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5111 5143 5111
Size: 32 9015
AlwNI CAG_nnn'CTG
Cuts at: 3457 5113 6352 7919 3457
Size: 1656 1239 1567 4585
Fragments arranged by size:
4585 1656 1567 1239
ApaLI G'TGCA_C
Cuts at: 7817 7817
Size: 9047
ApoI r'AATT_y
Cuts at: 257 803 829 1679 1876 2266 2980 3638 3821
Size: 546 26 850 197 390 714 658 183
Cuts at: 3821 3965 4048 4414 4471 4726 5019 5025 5980
Size: 144 83 366 57 255 293 6 955
Cuts at: 5980 6051 8400 8656 257
Size: 71 2349 256 648
Fragments arranged by size:
2349 955 850 714 658 648 546 390
366 293 256 255 197 183 144 83
71 57 26 6
AseI AT'TA_AT
Cuts at: 20 622 1129 1178 5050 20
Size: 602 507 49 3872 4017
Fragments arranged by size:
4017 3872 602 507 49
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9047
AvaI C'yCGr_G
Cuts at: 131 6067 131
Size: 5936 3111
AvrII C'CTAG_G
Cuts at: 1585 1585
Size: 9047
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3941 3974 3941
Size: 33 9014
BamHI G'GATC_C
Cuts at: 1157 6072 1157
Size: 4915 4132
BanI G'GyrC_C
Cuts at: 656 5283 6063 6477 6725 6760 656
Size: 4627 780 414 248 35 2943
Fragments arranged by size:
4627 2943 780 414 248 35
BanII G_rGCy'C
Cuts at: 6061 7091 6061
Size: 1030 8017
BbeI G_GCGC'C
Cuts at: 660 6481 6729 660
Size: 5821 248 2978
Fragments arranged by size:
5821 2978 248
BbsI GAAGACnn'nnnn_
Cuts at: 569 911 1569 3991 5297 5390 5815 5822 569
Size: 342 658 2422 1306 93 425 7 3794
Fragments arranged by size:
3794 2422 1306 658 425 342 93 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3682 3716 3682
Size: 34 9013
BciVI GTATCCnnnnn_n'
Cuts at: 1499 6945 7706 9047 1499
Size: 5446 761 1341 1499
Fragments arranged by size:
5446 1499 1341 761
BclI T'GATC_A
Cuts at: 6567 6567
Size: 9047
BfrBI ATG'CAT
Cuts at: 1644 1644
Size: 9047
BglII A'GATC_T
Cuts at: 5124 5619 6562 8599 5124
Size: 495 943 2037 5572
Fragments arranged by size:
5572 2037 943 495
BlpI GC'TnA_GC
Cuts at: 4640 6159 4640
Size: 1519 7528
Bme1580I G_kGCm'C
Cuts at: 4373 5288 6672 6765 7821 4373
Size: 915 1384 93 1056 5599
Fragments arranged by size:
5599 1384 1056 915 93
BmgBI CAC'GTC
Cuts at: 5959 5959
Size: 9047
BmrI ACTGGGnnnn_n'
Cuts at: 1574 2657 2992 3983 6259 6340 6675 8819 1574
Size: 1083 335 991 2276 81 335 2144 1802
Fragments arranged by size:
2276 2144 1802 1083 991 335 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 543 1100 7414 543
Size: 557 6314 2176
Fragments arranged by size:
6314 2176 557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 2077 173
Size: 1904 7143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1593 3599 6223 7102 7594 7892 513
Size: 1080 2006 2624 879 492 298 1668
Fragments arranged by size:
2624 2006 1668 1080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 108 226 1259 1634 8751 108
Size: 118 1033 375 7117 404
Fragments arranged by size:
7117 1033 404 375 118
BsaAI yAC'GTr
Cuts at: 253 787 6400 253
Size: 534 5613 2900
Fragments arranged by size:
5613 2900 534
BsaBI GATnn'nnATC
Cuts at: 6 1499 1707 1713 1719 1997 3014 6561 8598
Size: 1493 208 6 6 278 1017 3547 2037
Cuts at: 8598 6
Size: 455
Fragments arranged by size:
3547 2037 1493 1017 455 278 208 6
6
BsaHI Gr'CG_yC
Cuts at: 657 5842 6478 6726 7428 8924 657
Size: 5185 636 248 702 1496 780
Fragments arranged by size:
5185 1496 780 702 636 248
BsaWI w'CCGG_w
Cuts at: 3156 6236 6757 7435 7709 7856 8267 3156
Size: 3080 521 678 274 147 411 3936
Fragments arranged by size:
3936 3080 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 237 267 3611 3641 4854 4884 5111 5141 5234
Size: 30 3344 30 1213 30 227 30 93
Cuts at: 5234 5264 237
Size: 30 4020
Fragments arranged by size:
4020 3344 1213 227 93 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 149 3637 3709 4054 4517 4711 4862 5119 5467
Size: 3488 72 345 463 194 151 257 348
Cuts at: 5467 149
Size: 3729
Fragments arranged by size:
3729 3488 463 348 345 257 194 151
72
BseYI C'CCAG_C
Cuts at: 237 3505 6472 7230 7807 8303 237
Size: 3268 2967 758 577 496 981
Fragments arranged by size:
3268 2967 981 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4925 4925
Size: 9047
BsiEI CG_ry'CG
Cuts at: 104 6589 6635 7843 104
Size: 6485 46 1208 1308
Fragments arranged by size:
6485 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 165 252 6061 6839 7029 7821 165
Size: 87 5809 778 190 792 1391
Fragments arranged by size:
5809 1391 792 778 190 87
BsmI GAATG_Cn'
Cuts at: 647 3509 647
Size: 2862 6185
Bsp1286I G_dGCh'C
Cuts at: 165 252 4373 5288 6061 6672 6765 6839 7029
Size: 87 4121 915 773 611 93 74 190
Cuts at: 7029 7091 7821 165
Size: 62 730 1391
Fragments arranged by size:
4121 1391 915 773 730 611 190 93
87 74 62
BspEI T'CCGG_A
Cuts at: 8267 8267
Size: 9047
BspHI T'CATG_A
Cuts at: 375 4604 8223 375
Size: 4229 3619 1199
Fragments arranged by size:
4229 3619 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2998 3343 6097 6311 6613 6994 2998
Size: 345 2754 214 302 381 5051
Fragments arranged by size:
5051 2754 381 345 302 214
BsrBI CCG'CTC
Cuts at: 50 1155 7339 7393 8877 9042 50
Size: 1105 6184 54 1484 165 55
Fragments arranged by size:
6184 1484 1105 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2119 4157 5939 6959 2119
Size: 2038 1782 1020 4207
Fragments arranged by size:
4207 2038 1782 1020
BsrFI r'CCGG_y
Cuts at: 413 7045 7226 7435 413
Size: 6632 181 209 2025
Fragments arranged by size:
6632 2025 209 181
BsrGI T'GTAC_A
Cuts at: 3138 3138
Size: 9047
BssSI C'ACGA_G
Cuts at: 158 4874 7318 7676 158
Size: 4716 2444 358 1529
Fragments arranged by size:
4716 2444 1529 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2284 6424 2284
Size: 4140 4907
BstBI TT'CG_AA
Cuts at: 7408 7408
Size: 9047
BstEII G'GTnAC_C
Cuts at: 3319 3319
Size: 9047
BstYI r'GATC_y
Cuts at: 1 211 771 1157 1508 1708 1714 1720 4074
Size: 210 560 386 351 200 6 6 2354
Cuts at: 4074 4517 5124 5619 6072 6539 6562 6897 7143
Size: 443 607 495 453 467 23 335 246
Cuts at: 7143 8155 8241 8253 8599 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2354 1012 607 560 495 467 453 449
443 386 351 346 335 246 210 200
86 23 12 6 6
Bsu36I CC'TnA_GG
Cuts at: 4313 5855 5879 4313
Size: 1542 24 7481
Fragments arranged by size:
7481 1542 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6997 7178 7341 6997
Size: 181 163 8703
Fragments arranged by size:
8703 181 163
BtsI GCAGTG_nn'
Cuts at: 458 1953 4515 5343 5943 6290 458
Size: 1495 2562 828 600 347 3215
Fragments arranged by size:
3215 2562 1495 828 600 347
ClaI AT'CG_AT
Cuts at: 734 3132 734
Size: 2398 6649
DraI TTT'AAA
Cuts at: 2271 2903 3707 5222 5477 6046 2271
Size: 632 804 1515 255 569 5272
Fragments arranged by size:
5272 1515 804 632 569 255
DrdI GACnn_nn'nnGTC
Cuts at: 1478 2400 6753 7611 1478
Size: 922 4353 858 2914
Fragments arranged by size:
4353 2914 922 858
EaeI y'GGCC_r
Cuts at: 276 5987 6632 6806 7197 7224 8569 276
Size: 5711 645 174 391 27 1345 754
Fragments arranged by size:
5711 1345 754 645 391 174 27
EagI C'GGCC_G
Cuts at: 6632 6632
Size: 9047
EarI CTCTTCn'nnn_
Cuts at: 1315 3533 4311 4919 5171 5369 5411 5533 5729
Size: 2218 778 608 252 198 42 122 196
Cuts at: 5729 5826 5917 7070 7280 1315
Size: 97 91 1153 210 3082
Fragments arranged by size:
3082 2218 1153 778 608 252 210 198
196 122 97 91 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5410 7255 7565 7711 8857 5410
Size: 1845 310 146 1146 5600
Fragments arranged by size:
5600 1845 1146 310 146
EcoICRI GAG'CTC
Cuts at: 6059 6059
Size: 9047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 2077 2195 3154 3178 3235 3644 3863 3917
Size: 1904 118 959 24 57 409 219 54
Cuts at: 3917 5135 5393 5810 5834 6044 6872 7304 8051
Size: 1218 258 417 24 210 828 432 747
Cuts at: 8051 8319 173
Size: 268 901
Fragments arranged by size:
1904 1218 959 901 828 747 432 417
409 268 258 219 210 118 57 54
24 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2849 5134 2849
Size: 2285 6762
EcoO109I rG'GnC_Cy
Cuts at: 6186 6186
Size: 9047
EcoRI G'AATT_C
Cuts at: 257 3821 3965 6051 257
Size: 3564 144 2086 3253
Fragments arranged by size:
3564 3253 2086 144
EcoRV GAT'ATC
Cuts at: 2910 2910
Size: 9047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 718 793 2928 3184 3375 4555 5437 7066 718
Size: 75 2135 256 191 1180 882 1629 2699
Fragments arranged by size:
2699 2135 1629 1180 882 256 191 75
FspI TGC'GCA
Cuts at: 6828 6828
Size: 9047
HaeII r_GCGC'y
Cuts at: 660 1982 6481 6729 7751 8730 660
Size: 1322 4499 248 1022 979 977
Fragments arranged by size:
4499 1322 1022 979 977 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 171 203 1001 1033 1062 1094 1422 1454 1783
Size: 32 798 32 29 32 328 32 329
Cuts at: 1783 1815 2038 2070 3451 3483 3611 3643 4586
Size: 32 223 32 1381 32 128 32 943
Cuts at: 4586 4618 5371 5403 5752 5784 5943 5975 6889
Size: 32 753 32 349 32 159 32 914
Cuts at: 6889 6919 6921 6951 171
Size: 30 2 30 2267
Fragments arranged by size:
2267 1381 943 914 798 753 349 329
328 223 159 128 32 32 32 32
32 32 32 32 32 32 32 32
30 30 29 2
HincII GTy'rAC
Cuts at: 1041 1471 1483 3332 6086 1041
Size: 430 12 1849 2754 4002
Fragments arranged by size:
4002 2754 1849 430 12
HindIII A'AGCT_T
Cuts at: 2101 2591 4537 2101
Size: 490 1946 6611
Fragments arranged by size:
6611 1946 490
KasI G'GCGC_C
Cuts at: 656 6477 6725 656
Size: 5821 248 2978
Fragments arranged by size:
5821 2978 248
KpnI G_GTAC'C
Cuts at: 6067 6067
Size: 9047
MfeI C'AATT_G
Cuts at: 3428 3428
Size: 9047
MluI A'CGCG_T
Cuts at: 8480 8480
Size: 9047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 832 1103 1179 1354 3168 5427 5686 7718 7902
Size: 271 76 175 1814 2259 259 2032 184
Cuts at: 7902 832
Size: 1977
Fragments arranged by size:
2259 2032 1977 1814 271 259 184 175
76
MscI TGG'CCA
Cuts at: 278 5989 6808 278
Size: 5711 819 2517
Fragments arranged by size:
5711 2517 819
MslI CAynn'nnrTG
Cuts at: 1304 1386 7036 7163 1304
Size: 82 5650 127 3188
Fragments arranged by size:
5650 3188 127 82
MspA1I CmG'CkG
Cuts at: 4939 6158 6472 6832 7845 8090 4939
Size: 1219 314 360 1013 245 5896
Fragments arranged by size:
5896 1219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 7228 7228
Size: 9047
NarI GG'CG_CC
Cuts at: 657 6478 6726 657
Size: 5821 248 2978
Fragments arranged by size:
5821 2978 248
NdeI CA'TA_TG
Cuts at: 3326 3326
Size: 9047
NgoMIV G'CCGG_C
Cuts at: 7226 7226
Size: 9047
NsiI A_TGCA'T
Cuts at: 1646 1646
Size: 9047
NspI r_CATG'y
Cuts at: 748 1198 1303 1653 3575 6100 6600 7507 748
Size: 450 105 350 1922 2525 500 907 2288
Fragments arranged by size:
2525 2288 1922 907 500 450 350 105
PciI A'CATG_T
Cuts at: 1194 1649 3571 7503 1194
Size: 455 1922 3932 2738
Fragments arranged by size:
3932 2738 1922 455
PflMI CCAn_nnn'nTGG
Cuts at: 2164 2896 2164
Size: 732 8315
PfoI T'CCnGG_A
Cuts at: 189 2352 189
Size: 2163 6884
PmeI GTTT'AAAC
Cuts at: 6046 6046
Size: 9047
PmlI CAC'GTG
Cuts at: 253 253
Size: 9047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 8186 8218 5111
Size: 32 3043 32 5940
Fragments arranged by size:
5940 3043 32 32
PsiI TTA'TAA
Cuts at: 2146 3079 2146
Size: 933 8114
PspXI vC'TCGA_Gb
Cuts at: 131 131
Size: 9047
PstI C_TGCA'G
Cuts at: 4170 6094 6779 4170
Size: 1924 685 6438
Fragments arranged by size:
6438 1924 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9047
PvuII CAG'CTG
Cuts at: 4939 6472 6832 4939
Size: 1533 360 7154
Fragments arranged by size:
7154 1533 360
RsrII CG'GwC_CG
Cuts at: 7242 7242
Size: 9047
SacI G_AGCT'C
Cuts at: 6061 6061
Size: 9047
SalI G'TCGA_C
Cuts at: 1469 6084 1469
Size: 4615 4432
SapI GCTCTTCn'nnn_
Cuts at: 3533 5171 5411 7070 7280 3533
Size: 1638 240 1659 210 5300
Fragments arranged by size:
5300 1659 1638 240 210
SbfI CC_TGCA'GG
Cuts at: 6094 6094
Size: 9047
ScaI AGT'ACT
Cuts at: 3501 8564 3501
Size: 5063 3984
SfcI C'TryA_G
Cuts at: 33 2510 2531 2861 3890 4166 4325 5312 6090
Size: 2477 21 330 1029 276 159 987 778
Cuts at: 6090 6775 7768 7959 33
Size: 685 993 191 1121
Fragments arranged by size:
2477 1121 1029 993 987 778 685 330
276 191 159 21
SfoI GGC'GCC
Cuts at: 658 6479 6727 658
Size: 5821 248 2978
Fragments arranged by size:
5821 2978 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9047
SmaI CCC'GGG
Cuts at: 6069 6069
Size: 9047
SmlI C'TyrA_G
Cuts at: 131 528 1608 3614 6202 7117 7609 7871 131
Size: 397 1080 2006 2588 915 492 262 1307
Fragments arranged by size:
2588 2006 1307 1080 915 492 397 262
SnaBI TAC'GTA
Cuts at: 787 787
Size: 9047
SpeI A'CTAG_T
Cuts at: 67 4234 4243 67
Size: 4167 9 4871
Fragments arranged by size:
4871 4167 9
SphI G_CATG'C
Cuts at: 748 6100 6600 748
Size: 5352 500 3195
Fragments arranged by size:
5352 3195 500
SspI AAT'ATT
Cuts at: 1376 3315 3929 4005 1376
Size: 1939 614 76 6418
Fragments arranged by size:
6418 1939 614 76
StuI AGG'CCT
Cuts at: 186 207 5740 186
Size: 21 5533 3493
Fragments arranged by size:
5533 3493 21
StyI C'CwwG_G
Cuts at: 1585 2501 3083 4409 6181 6535 1585
Size: 916 582 1326 1772 354 4097
Fragments arranged by size:
4097 1772 1326 916 582 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 941 1241 1616 6630 6763 7222 7431 941
Size: 300 375 5014 133 459 209 2557
Fragments arranged by size:
5014 2557 459 375 300 209 133
TatI w'GTAC_w
Cuts at: 498 1851 2170 2297 3138 3177 3499 5953 8562
Size: 1353 319 127 841 39 322 2454 2609
Cuts at: 8562 498
Size: 983
Fragments arranged by size:
2609 2454 1353 983 841 322 319 127
39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 756 788 2321 2353 4641 4673 8564
Size: 32 487 32 1533 32 2288 32 3891
Cuts at: 8564 8596 237
Size: 32 688
Fragments arranged by size:
3891 2288 1533 688 487 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 6844 6844
Size: 9047
XbaI T'CTAG_A
Cuts at: 1594 1699 2631 3825 6078 1594
Size: 105 932 1194 2253 4563
Fragments arranged by size:
4563 2253 1194 932 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 4449 6542 363
Size: 4086 2093 2868
Fragments arranged by size:
4086 2868 2093
XhoI C'TCGA_G
Cuts at: 131 131
Size: 9047
XmaI C'CCGG_G
Cuts at: 6067 6067
Size: 9047
XmnI GAAnn'nnTTC
Cuts at: 1566 3638 1566
Size: 2072 6975
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII ApaI AscI BbvCI BglI BmtI
Bpu10I BsiWI BsmBI BssHII BstXI BstZ17I BtgI CspCI
DraIII FseI FspAI HpaI NcoI NheI NotI NruI
PacI PasI PpuMI PshAI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI ZraI