Restriction map for pF1KB6004
 (Linear) (Six-base) MAPSORT of: KIBB6004.seq  Check: 2976  from: 1  to: 5934



KIBB6004.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0    1362    5934 
   Size:       1362    4572
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2087    3046    3070    3127    3536    3755    3809    5027 
   Size:       2087     959      24      57     409     219      54    1218

Cuts at:   5027    5285    5702    5726    5934 
   Size:        258     417      24     208

  Fragments arranged by size: 

                2087    1218     959     417     409     258     219     208
                  57      54      24      24
 
AfeI AGC'GCT

Cuts at:      0    1872    5934 
   Size:       1872    4062
 
AflIII A'CryG_T

Cuts at:      0    1086    1541    3463    5848    5934 
   Size:       1086     455    1922    2385      86

  Fragments arranged by size: 

                2385    1922    1086     455      86
 
AhdI GACnn_n'nnGTC

Cuts at:      0     907     968    1944    5934 
   Size:        907      61     976    3990

  Fragments arranged by size: 

                3990     976     907      61
 
AleI CACnn'nnGTG

Cuts at:      0    1278    5934 
   Size:       1278    4656
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5251    5934 
   Size:       5251     683
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    5003    5035    5934 
   Size:       5003      32     899

  Fragments arranged by size: 

                5003     899      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3349    5005    5934 
   Size:       3349    1656     929

  Fragments arranged by size: 

                3349    1656     929
 
ApoI r'AATT_y

Cuts at:      0     149     695     721    1571    1768    2158    2872    3530 
   Size:        149     546      26     850     197     390     714     658

Cuts at:   3530    3713    3857    3940    4306    4363    4618    4911    4917 
   Size:        183     144      83     366      57     255     293       6

Cuts at:   4917    5872    5934 
   Size:        955      62

  Fragments arranged by size: 

                 955     850     714     658     546     390     366     293
                 255     197     183     149     144      83      62      57
                  26       6
 
AseI AT'TA_AT

Cuts at:      0     514    1021    1070    4942    5934 
   Size:        514     507      49    3872     992

  Fragments arranged by size: 

                3872     992     514     507      49
 
AvaI C'yCGr_G

Cuts at:      0      23    5934 
   Size:         23    5911
 
AvrII C'CTAG_G

Cuts at:      0    1477    5934 
   Size:       1477    4457
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3833    3866    5934 
   Size:       3833      33    2068

  Fragments arranged by size: 

                3833    2068      33
 
BamHI G'GATC_C

Cuts at:      0    1049    5934 
   Size:       1049    4885
 
BanI G'GyrC_C

Cuts at:      0     548    5175    5934 
   Size:        548    4627     759

  Fragments arranged by size: 

                4627     759     548
 
BbeI G_GCGC'C

Cuts at:      0     552    5934 
   Size:        552    5382
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     461     803    1461    3883    5189    5282    5707    5714 
   Size:        461     342     658    2422    1306      93     425       7

Cuts at:   5714    5934 
   Size:        220

  Fragments arranged by size: 

                2422    1306     658     461     425     342     220      93
                   7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3574    3608    5934 
   Size:       3574      34    2326

  Fragments arranged by size: 

                3574    2326      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1391    5934 
   Size:       1391    4543
 
BfrBI ATG'CAT

Cuts at:      0    1536    5934 
   Size:       1536    4398
 
BglII A'GATC_T

Cuts at:      0    5016    5511    5934 
   Size:       5016     495     423

  Fragments arranged by size: 

                5016     495     423
 
BlpI GC'TnA_GC

Cuts at:      0    4532    5934 
   Size:       4532    1402
 
Bme1580I G_kGCm'C

Cuts at:      0    4265    5180    5934 
   Size:       4265     915     754

  Fragments arranged by size: 

                4265     915     754
 
BmgBI CAC'GTC

Cuts at:      0    5851    5934 
   Size:       5851      83
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1466    2549    2884    3875    5934 
   Size:       1466    1083     335     991    2059

  Fragments arranged by size: 

                2059    1466    1083     991     335
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     435     992    5934 
   Size:        435     557    4942

  Fragments arranged by size: 

                4942     557     435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65    1969    5934 
   Size:         65    1904    3965

  Fragments arranged by size: 

                3965    1904      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405    1485    3491    5934 
   Size:        405    1080    2006    2443

  Fragments arranged by size: 

                2443    2006    1080     405
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     118    1151    1526    5934 
   Size:        118    1033     375    4408

  Fragments arranged by size: 

                4408    1033     375     118
 
BsaAI yAC'GTr

Cuts at:      0     145     679    5934 
   Size:        145     534    5255

  Fragments arranged by size: 

                5255     534     145
 
BsaBI GATnn'nnATC

Cuts at:      0    1391    1599    1605    1611    1889    2906    5934 
   Size:       1391     208       6       6     278    1017    3028

  Fragments arranged by size: 

                3028    1391    1017     278     208       6       6
 
BsaHI Gr'CG_yC

Cuts at:      0     549    5734    5934 
   Size:        549    5185     200

  Fragments arranged by size: 

                5185     549     200
 
BsaWI w'CCGG_w

Cuts at:      0    3048    5934 
   Size:       3048    2886
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     129     159    3503    3533    4746    4776    5003    5033 
   Size:        129      30    3344      30    1213      30     227      30

Cuts at:   5033    5126    5156    5934 
   Size:         93      30     778

  Fragments arranged by size: 

                3344    1213     778     227     129      93      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      41    3529    3601    3946    4409    4603    4754    5011 
   Size:         41    3488      72     345     463     194     151     257

Cuts at:   5011    5359    5934 
   Size:        348     575

  Fragments arranged by size: 

                3488     575     463     348     345     257     194     151
                  72      41
 
BseYI C'CCAG_C

Cuts at:      0     129    3397    5934 
   Size:        129    3268    2537

  Fragments arranged by size: 

                3268    2537     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4817    5934 
   Size:       4817    1117
 
BsiHKAI G_wGCw'C

Cuts at:      0      57     144    5934 
   Size:         57      87    5790

  Fragments arranged by size: 

                5790      87      57
 
BsmI GAATG_Cn'

Cuts at:      0     539    3401    5934 
   Size:        539    2862    2533

  Fragments arranged by size: 

                2862    2533     539
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     144    4265    5180    5934 
   Size:         57      87    4121     915     754

  Fragments arranged by size: 

                4121     915     754      87      57
 
BspHI T'CATG_A

Cuts at:      0     267    4496    5934 
   Size:        267    4229    1438

  Fragments arranged by size: 

                4229    1438     267
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2890    3235    5934 
   Size:       2890     345    2699

  Fragments arranged by size: 

                2890    2699     345
 
BsrBI CCG'CTC

Cuts at:      0    1047    5934 
   Size:       1047    4887
 
BsrDI GCAATG_nn'

Cuts at:      0    2011    4049    5831    5934 
   Size:       2011    2038    1782     103

  Fragments arranged by size: 

                2038    2011    1782     103
 
BsrFI r'CCGG_y

Cuts at:      0     305    5934 
   Size:        305    5629
 
BsrGI T'GTAC_A

Cuts at:      0    3030    5934 
   Size:       3030    2904
 
BssSI C'ACGA_G

Cuts at:      0      50    4766    5934 
   Size:         50    4716    1168

  Fragments arranged by size: 

                4716    1168      50
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2176    5934 
   Size:       2176    3758
 
BstEII G'GTnAC_C

Cuts at:      0    3211    5934 
   Size:       3211    2723
 
BstYI r'GATC_y

Cuts at:      0     103     663    1049    1400    1600    1606    1612    3966 
   Size:        103     560     386     351     200       6       6    2354

Cuts at:   3966    4409    5016    5511    5934 
   Size:        443     607     495     423

  Fragments arranged by size: 

                2354     607     560     495     443     423     386     351
                 200     103       6       6
 
Bsu36I CC'TnA_GG

Cuts at:      0    4205    5747    5771    5934 
   Size:       4205    1542      24     163

  Fragments arranged by size: 

                4205    1542     163      24
 
BtsI GCAGTG_nn'

Cuts at:      0     350    1845    4407    5235    5835    5934 
   Size:        350    1495    2562     828     600      99

  Fragments arranged by size: 

                2562    1495     828     600     350      99
 
ClaI AT'CG_AT

Cuts at:      0     626    3024    5934 
   Size:        626    2398    2910

  Fragments arranged by size: 

                2910    2398     626
 
DraI TTT'AAA

Cuts at:      0    2163    2795    3599    5114    5369    5934 
   Size:       2163     632     804    1515     255     565

  Fragments arranged by size: 

                2163    1515     804     632     565     255
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1370    2292    5934 
   Size:       1370     922    3642

  Fragments arranged by size: 

                3642    1370     922
 
EaeI y'GGCC_r

Cuts at:      0     168    5879    5934 
   Size:        168    5711      55

  Fragments arranged by size: 

                5711     168      55
 
EarI CTCTTCn'nnn_

Cuts at:      0    1207    3425    4203    4811    5063    5261    5303    5425 
   Size:       1207    2218     778     608     252     198      42     122

Cuts at:   5425    5621    5718    5809    5934 
   Size:        196      97      91     125

  Fragments arranged by size: 

                2218    1207     778     608     252     198     196     125
                 122      97      91      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    5302    5934 
   Size:       5302     632
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65    1969    2087    3046    3070    3127    3536    3755 
   Size:         65    1904     118     959      24      57     409     219

Cuts at:   3755    3809    5027    5285    5702    5726    5934 
   Size:         54    1218     258     417      24     208

  Fragments arranged by size: 

                1904    1218     959     417     409     258     219     208
                 118      65      57      54      24      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2741    5026    5934 
   Size:       2741    2285     908

  Fragments arranged by size: 

                2741    2285     908
 
EcoRI G'AATT_C

Cuts at:      0     149    3713    3857    5934 
   Size:        149    3564     144    2077

  Fragments arranged by size: 

                3564    2077     149     144
 
EcoRV GAT'ATC

Cuts at:      0    2802    5934 
   Size:       2802    3132
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     610     685    2820    3076    3267    4447    5329    5934 
   Size:        610      75    2135     256     191    1180     882     605

  Fragments arranged by size: 

                2135    1180     882     610     605     256     191      75
            
 
HaeII r_GCGC'y

Cuts at:      0     552    1874    5934 
   Size:        552    1322    4060

  Fragments arranged by size: 

                4060    1322     552
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      63      95     893     925     954     986    1314    1346 
   Size:         63      32     798      32      29      32     328      32

Cuts at:   1346    1675    1707    1930    1962    3343    3375    3503    3535 
   Size:        329      32     223      32    1381      32     128      32

Cuts at:   3535    4478    4510    5263    5295    5644    5676    5835    5867 
   Size:        943      32     753      32     349      32     159      32

Cuts at:   5867    5934 
   Size:         67

  Fragments arranged by size: 

                1381     943     798     753     349     329     328     223
                 159     128      67      63      32      32      32      32
                  32      32      32      32      32      32      32      32
                  29
 
HincII GTy'rAC

Cuts at:      0     933    1363    1375    3224    5934 
   Size:        933     430      12    1849    2710

  Fragments arranged by size: 

                2710    1849     933     430      12
 
HindIII A'AGCT_T

Cuts at:      0    1993    2483    4429    5934 
   Size:       1993     490    1946    1505

  Fragments arranged by size: 

                1993    1946    1505     490
 
KasI G'GCGC_C

Cuts at:      0     548    5934 
   Size:        548    5386
 
MfeI C'AATT_G

Cuts at:      0    3320    5934 
   Size:       3320    2614
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     724     995    1071    1246    3060    5319    5578    5934 
   Size:        724     271      76     175    1814    2259     259     356

  Fragments arranged by size: 

                2259    1814     724     356     271     259     175      76
            
 
MscI TGG'CCA

Cuts at:      0     170    5881    5934 
   Size:        170    5711      53

  Fragments arranged by size: 

                5711     170      53
 
MslI CAynn'nnrTG

Cuts at:      0    1196    1278    5934 
   Size:       1196      82    4656

  Fragments arranged by size: 

                4656    1196      82
 
MspA1I CmG'CkG

Cuts at:      0    4831    5934 
   Size:       4831    1103
 
NarI GG'CG_CC

Cuts at:      0     549    5934 
   Size:        549    5385
 
NdeI CA'TA_TG

Cuts at:      0    3218    5934 
   Size:       3218    2716
 
NsiI A_TGCA'T

Cuts at:      0    1538    5934 
   Size:       1538    4396
 
NspI r_CATG'y

Cuts at:      0     640    1090    1195    1545    3467    5934 
   Size:        640     450     105     350    1922    2467

  Fragments arranged by size: 

                2467    1922     640     450     350     105
 
PciI A'CATG_T

Cuts at:      0    1086    1541    3463    5934 
   Size:       1086     455    1922    2471

  Fragments arranged by size: 

                2471    1922    1086     455
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2056    2788    5934 
   Size:       2056     732    3146

  Fragments arranged by size: 

                3146    2056     732
 
PfoI T'CCnGG_A

Cuts at:      0      81    2244    5934 
   Size:         81    2163    3690

  Fragments arranged by size: 

                3690    2163      81
 
PmlI CAC'GTG

Cuts at:      0     145    5934 
   Size:        145    5789
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5003    5035    5934 
   Size:       5003      32     899

  Fragments arranged by size: 

                5003     899      32
 
PsiI TTA'TAA

Cuts at:      0    2038    2971    5934 
   Size:       2038     933    2963

  Fragments arranged by size: 

                2963    2038     933
 
PspXI vC'TCGA_Gb

Cuts at:      0      23    5934 
   Size:         23    5911
 
PstI C_TGCA'G

Cuts at:      0    4062    5934 
   Size:       4062    1872
 
PvuII CAG'CTG

Cuts at:      0    4831    5934 
   Size:       4831    1103
 
SalI G'TCGA_C

Cuts at:      0    1361    5934 
   Size:       1361    4573
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3425    5063    5303    5934 
   Size:       3425    1638     240     631

  Fragments arranged by size: 

                3425    1638     631     240
 
ScaI AGT'ACT

Cuts at:      0    3393    5934 
   Size:       3393    2541
 
SfcI C'TryA_G

Cuts at:      0    2402    2423    2753    3782    4058    4217    5204    5934 
   Size:       2402      21     330    1029     276     159     987     730

  Fragments arranged by size: 

                2402    1029     987     730     330     276     159      21
            
 
SfoI GGC'GCC

Cuts at:      0     550    5934 
   Size:        550    5384
 
SmlI C'TyrA_G

Cuts at:      0      23     420    1500    3506    5934 
   Size:         23     397    1080    2006    2428

  Fragments arranged by size: 

                2428    2006    1080     397      23
 
SnaBI TAC'GTA

Cuts at:      0     679    5934 
   Size:        679    5255
 
SpeI A'CTAG_T

Cuts at:      0    4126    4135    5934 
   Size:       4126       9    1799

  Fragments arranged by size: 

                4126    1799       9
 
SphI G_CATG'C

Cuts at:      0     640    5934 
   Size:        640    5294
 
SspI AAT'ATT

Cuts at:      0    1268    3207    3821    3897    5934 
   Size:       1268    1939     614      76    2037

  Fragments arranged by size: 

                2037    1939    1268     614      76
 
StuI AGG'CCT

Cuts at:      0      78      99    5632    5934 
   Size:         78      21    5533     302

  Fragments arranged by size: 

                5533     302      78      21
 
StyI C'CwwG_G

Cuts at:      0    1477    2393    2975    4301    5934 
   Size:       1477     916     582    1326    1633

  Fragments arranged by size: 

                1633    1477    1326     916     582
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     833    1133    1508    5934 
   Size:        833     300     375    4426

  Fragments arranged by size: 

                4426     833     375     300
 
TatI w'GTAC_w

Cuts at:      0     390    1743    2062    2189    3030    3069    3391    5845 
   Size:        390    1353     319     127     841      39     322    2454

Cuts at:   5845    5934 
   Size:         89

  Fragments arranged by size: 

                2454    1353     841     390     322     319     127      89
                  39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     129     161     648     680    2213    2245    4533    4565 
   Size:        129      32     487      32    1533      32    2288      32

Cuts at:   4565    5934 
   Size:       1369

  Fragments arranged by size: 

                2288    1533    1369     487     129      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0    1486    1591    2523    3717    5934 
   Size:       1486     105     932    1194    2217

  Fragments arranged by size: 

                2217    1486    1194     932     105
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255    4341    5934 
   Size:        255    4086    1593

  Fragments arranged by size: 

                4086    1593     255
 
XhoI C'TCGA_G

Cuts at:      0      23    5934 
   Size:         23    5911
 
XmnI GAAnn'nnTTC

Cuts at:      0    1458    3530    5934 
   Size:       1458    2072    2404

  Fragments arranged by size: 

                2404    2072    1458
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstYI   Bsu36I     BtsI     ClaI     DraI     DrdI
     EaeI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NdeI     NsiI     NspI     PciI    PflMI
     PfoI     PmlI     PpiI     PsiI    PspXI     PstI    PvuII     SalI
     SapI     ScaI     SfcI     SfoI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     ApaI    ApaLI
     AscI    AsiSI    BanII    BbvCI     BclI     BglI     BmtI   Bpu10I
    BsiEI    BsiWI    BsmBI    BspEI   BssHII    BstBI    BstXI  BstZ17I
     BtgI    BtgZI    CspCI   DraIII     EagI  EcoICRI EcoO109I     FseI
     FspI    FspAI     HpaI     KpnI     MluI     NaeI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PmeI    PpuMI    PshAI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SrfI     SwaI  Tth111I
     XmaI     ZraI
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