Restriction map for pF1KB5637
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AspI        AvrII       BalI        BbeI        
BcgI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsgI        
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HaeII       HpaI        KasI        KspI        MamI        
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         203  GACGTC               GACGT^C
Acc65I       1086  GGTACC               G^GTACC
AcyI          203  GACGTC               G[GA]^CG[CT]C
AflIII        460  ACACGT               A^C[GA][CT]GT
AhdI         2362  GACAGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          267  GGGCCC               GGGCC^C
Asp700I      1926  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1086  GGTACC               G^GTACC
AspEI        2362  GACAGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         876  TGCGCA               TGC^GCA
BbvCI        1045  CCTCAGC              CCTCAGC(-5/-2)
BclI         2055  TGATCA               T^GATCA
BglI         1489  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         203  GACGTC               G[GA]^CG[CT]C
BsiEI         190  CGGCCG               CG[GA][CT]^CG
BsmI          353  GAATGC               GAATGC(1/-1)
BsrBI        1561  GAGCGG               CCGCTC(-3/-3)
BssSI        3213  CACGAG               CACGAG(-5/-1)
BstAPI       1060  GCAATACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2298  TTCGAA               TT^CGAA
DrdI         1713  GACAATGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          190  CGGCCG               C^GGCCG
Eam1105I     2362  GACAGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         190  CGGCCG               C^GGCCG
Eco52I        190  CGGCCG               C^GGCCG
FbaI         2055  TGATCA               T^GATCA
FspI          876  TGCGCA               TGC^GCA
HgaI         2997  GACGC                GACGC(5/10)
Hin1I         203  GACGTC               G[GA]^CG[CT]C
HincII       1639  GTTGAC               GT[CT]^[GA]AC
HindII       1639  GTTGAC               GT[CT]^[GA]AC
HindIII      2767  AAGCTT               A^AGCTT
KpnI         1086  GGTACC               GGTAC^C
MfeI         2222  CAATTG               C^AATTG
MunI         2222  CAATTG               C^AATTG
NdeI         3155  CATATG               CA^TATG
NspV         2298  TTCGAA               TT^CGAA
PflMI         151  CCACCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2704  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          803  CTGCAG               CTGCA^G
SacI           52  GAGCTC               GAGCT^C
ScaI         3111  AGTACT               AGT^ACT
SfuI         2298  TTCGAA               TT^CGAA
SmaI           45  CCCGGG               CCC^GGG
SmlI           78  CTTGAG               C^T[CT][GA]AG
StuI           74  AGGCCT               AGG^CCT
Van91I        151  CCACCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1901  CCATTGTCCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          45  CCCGGG               C^CCGGG
XmaI           45  CCCGGG               C^CCGGG
XmnI         1926  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI          52  GAGCTC               G[AT]GC[AT]^C
             2812  GAGCAC              
AvaI           45  CCCGGG               C^[CT]CG[GA]G
             1889  CCCGAG              
BamHI        1413  GGATCC               G^GATCC
             2737  GGATCC              
BanI         1086  GGTACC               G^G[CT][GA]CC
             1700  GGCACC              
BbrPI         461  CACGTG               CAC^GTG
             2862  CACGTG              
BciVI         251  GGATAC               GTATCC(6/5)
              808  GTATCC              
BsaAI         461  CACGTG               [CT]AC^GT[GA]
             2862  CACGTG              
BsaI          285  GAGACC               GGTCTC(1/5)
             3010  GAGACC              
BsiHKAI        52  GAGCTC               G[AT]GC[AT]^C
             2812  GAGCAC              
BsoBI          45  CCCGGG               C^[CT]CG[GA]G
             1889  CCCGAG              
EaeI          190  CGGCCG               [CT]^GGCC[GA]
             2267  TGGCCG              
EcoNI         238  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              241  CCTCCAGGAGG         
MslI          978  CACACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2858  CATCCACGTG          
MspA1I       2270  CCGCTG               C[AC]G^C[GT]G
             2724  CCGCTG              
NcoI         1534  CCATGG               C^CATGG
             1865  CCATGG              
PleI         1846  GACTC                GAGTC(4/5)
             2491  GACTC               
PmaCI         461  CACGTG               CAC^GTG
             2862  CACGTG              
PmlI          461  CACGTG               CAC^GTG
             2862  CACGTG              
SphI         1115  GCATGC               GCATG^C
             2196  GCATGC              
Tsp45I        364  GTGAC                ^GT[GC]AC
             2924  GTGAC               
TspRI         113  CAGTG                CA[GC]TG[ACGT][ACGT]^
             2922  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PshBI       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TthHB8I     XhoII       
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