Restriction map for pF1KB8765
 (Linear) (Six-base) MAPSORT of: KIBB8765.seq  Check: 9286  from: 1  to: 669



KIBB8765.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     372     669 
   Size:        372     297
 
AccI GT'mk_AC

Cuts at:      0     182     669 
   Size:        182     487
 
AhdI GACnn_n'nnGTC

Cuts at:      0      53     669 
   Size:         53     616
 
AlwNI CAG_nnn'CTG

Cuts at:      0     294     636     669 
   Size:        294     342      33

  Fragments arranged by size: 

                 342     294      33
 
ApaI G_GGCC'C

Cuts at:      0     327     669 
   Size:        327     342
 
ApoI r'AATT_y

Cuts at:      0      94     669 
   Size:         94     575
 
AvaI C'yCGr_G

Cuts at:      0      63     669 
   Size:         63     606
 
BanI G'GyrC_C

Cuts at:      0     299     669 
   Size:        299     370
 
BanII G_rGCy'C

Cuts at:      0     327     416     669 
   Size:        327      89     253

  Fragments arranged by size: 

                 327     253      89
 
BbeI G_GCGC'C

Cuts at:      0     303     669 
   Size:        303     366
 
BbvCI CC'TCA_GC

Cuts at:      0     295     669 
   Size:        295     374
 
BglI GCCn_nnn'nGGC

Cuts at:      0     401     669 
   Size:        401     268
 
Bme1580I G_kGCm'C

Cuts at:      0     327     669 
   Size:        327     342
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95     669 
   Size:         95     574
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     182     669 
   Size:        131      51     487

  Fragments arranged by size: 

                 487     131      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     295     334     669 
   Size:        295      39     335

  Fragments arranged by size: 

                 335     295      39
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     669 
   Size:        382     287
 
BsaHI Gr'CG_yC

Cuts at:      0     300     439     669 
   Size:        300     139     230

  Fragments arranged by size: 

                 300     230     139
 
BseYI C'CCAG_C

Cuts at:      0     139     232     433     489     513     557     669 
   Size:        139      93     201      56      24      44     112

  Fragments arranged by size: 

                 201     139     112      93      56      44      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     369     669 
   Size:        111     258     300

  Fragments arranged by size: 

                 300     258     111
 
Bsp1286I G_dGCh'C

Cuts at:      0     327     416     669 
   Size:        327      89     253

  Fragments arranged by size: 

                 327     253      89
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     372     669 
   Size:        372     297
 
BstEII G'GTnAC_C

Cuts at:      0      27     669 
   Size:         27     642
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     669 
   Size:        147     522
 
Bsu36I CC'TnA_GG

Cuts at:      0     428     669 
   Size:        428     241
 
EarI CTCTTCn'nnn_

Cuts at:      0     503     669 
   Size:        503     166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     182     669 
   Size:        131      51     487

  Fragments arranged by size: 

                 487     131      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60     323     324     633     669 
   Size:         60     263       1     309      36

  Fragments arranged by size: 

                 309     263      60      36       1
 
EcoRI G'AATT_C

Cuts at:      0      94     669 
   Size:         94     575
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     190     669 
   Size:        190     479
 
HaeII r_GCGC'y

Cuts at:      0     303     669 
   Size:        303     366
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      39      71     250     282     429     461     669 
   Size:         39      32     179      32     147      32     208

  Fragments arranged by size: 

                 208     179     147      39      32      32      32
 
HincII GTy'rAC

Cuts at:      0     183     669 
   Size:        183     486
 
KasI G'GCGC_C

Cuts at:      0     299     669 
   Size:        299     370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     669 
   Size:        598      71
 
MslI CAynn'nnrTG

Cuts at:      0     345     669 
   Size:        345     324
 
MspA1I CmG'CkG

Cuts at:      0     489     669 
   Size:        489     180
 
NarI GG'CG_CC

Cuts at:      0     300     669 
   Size:        300     369
 
NdeI CA'TA_TG

Cuts at:      0     374     669 
   Size:        374     295
 
NspI r_CATG'y

Cuts at:      0     240     669 
   Size:        240     429
 
PspOMI G'GGCC_C

Cuts at:      0     323     669 
   Size:        323     346
 
PstI C_TGCA'G

Cuts at:      0     290     669 
   Size:        290     379
 
PvuII CAG'CTG

Cuts at:      0     489     669 
   Size:        489     180
 
SalI G'TCGA_C

Cuts at:      0     181     669 
   Size:        181     488
 
SapI GCTCTTCn'nnn_

Cuts at:      0     503     669 
   Size:        503     166
 
SbfI CC_TGCA'GG

Cuts at:      0     290     669 
   Size:        290     379
 
SfcI C'TryA_G

Cuts at:      0     286     669 
   Size:        286     383
 
SfoI GGC'GCC

Cuts at:      0     301     669 
   Size:        301     368
 
SmlI C'TyrA_G

Cuts at:      0     397     669 
   Size:        397     272
 
StuI AGG'CCT

Cuts at:      0     604     669 
   Size:        604      65
 
StyI C'CwwG_G

Cuts at:      0     455     669 
   Size:        455     214
 
TatI w'GTAC_w

Cuts at:      0     218     669 
   Size:        218     451
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     120     152     669 
   Size:        120      32     517

  Fragments arranged by size: 

                 517     120      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      33     669 
   Size:         33     636
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106     669 
   Size:        106     563
 
XmnI GAAnn'nnTTC

Cuts at:      0     625     669 
   Size:        625      44
 

 Enzymes that do cut:

     AarI     AccI     AhdI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI    BbvCI     BglI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaHI    BseYI     BsgI Bsp1286I    BspMI   BstEII    BstXI
   Bsu36I     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII     KasI     MmeI     MslI   MspA1I     NarI     NdeI     NspI
   PspOMI     PstI    PvuII     SalI     SapI     SbfI     SfcI     SfoI
     SmlI     StuI     StyI     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     NaeI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     XbaI     XhoI     XmaI     ZraI
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