Restriction map for pF1KB4196
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AccI        AccIII      AflIII      AgeI        
Aor51HI     AscI        AseI        AsnI        AspI        
AviII       AvrII       BbsI        BclI        BglI        
BglII       BlnI        BpuAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        KspI        MamI        
MluI        MroI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1865  GGTACC               G^GTACC
AflII         994  CTTAAG               C^TTAAG
AhdI         2221  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        910  GTGCAC               G^TGCAC
AocI          112  CCTCAGG              CC^T[ACGT]AGG
ApaI          465  GGGCCC               GGGCC^C
ApaLI         910  GTGCAC               G^TGCAC
Asp718I      1865  GGTACC               G^GTACC
AspEI        2221  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         578  GGATCC               G^GATCC
BbeI         2411  GGCGCC               GGCGC^C
BbrPI        1318  CACGTG               CAC^GTG
BcgI           94  CGAAGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2519  GTATCC               GTATCC(6/5)
BfrI          994  CTTAAG               C^TTAAG
BsaAI        1318  CACGTG               [CT]AC^GT[GA]
BsrFI        2115  GCCGGC               [GA]^CCGG[CT]
BstEII       1791  GGTCACC              G^GT[ACGT]ACC
BstPI        1791  GGTCACC              G^GT[ACGT]ACC
BstXI        1271  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        112  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       2115  GCCGGC               [GA]^CCGG[CT]
DraIII       1406  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1763  CCATGG               C^C[GA][CT]GG
Eam1105I     2221  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        112  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2059  CCTCAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1791  GGTCACC              G^GT[ACGT]ACC
HindIII       316  AAGCTT               A^AGCTT
KasI         2411  GGCGCC               G^GCGCC
KpnI         1865  GGTACC               GGTAC^C
MfeI         1428  CAATTG               C^AATTG
MunI         1428  CAATTG               C^AATTG
NaeI         2115  GCCGGC               GCC^GGC
NarI         2411  GGCGCC               GG^CGCC
NcoI         1763  CCATGG               C^CATGG
NgoMIV       2115  GCCGGC               G^CCGGC
PmaCI        1318  CACGTG               CAC^GTG
PmlI         1318  CACGTG               CAC^GTG
SexAI        1256  ACCTGGT              A^CC[AT]GGT
SfoI         2411  GGCGCC               GGC^GCC
SmaI         2708  CCCGGG               CCC^GGG
TaqI         2567  TCGA                 T^CGA
TthHB8I      2567  TCGA                 T^CGA
XmaCI        2708  CCCGGG               C^CCGGG
XmaI         2708  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII         280  CGCG                 CG^CG
             2552  CGCG                
AclI         1367  AACGTT               AA^CGTT
             1888  AACGTT              
AvaI          461  CTCGGG               C^[CT]CG[GA]G
             2708  CCCGGG              
BsaWI        1868  ACCGGA               [AT]^CCGG[AT]
             2387  ACCGGA              
BsgI          140  CTGCAC               GTGCAG(16/14)
             1945  CTGCAC              
BsmBI        2110  GAGACG               CGTCTC(1/5)
             2548  GAGACG              
BsoBI         461  CTCGGG               C^[CT]CG[GA]G
             2708  CCCGGG              
BspHI          48  TCATGA               T^CATGA
             1605  TCATGA              
BsrBI        2031  CCGCTC               CCGCTC(-3/-3)
              679  GAGCGG              
BstUI         280  CGCG                 CG^CG
             2552  CGCG                
EcoRI         841  GAATTC               G^AATTC
              927  GAATTC              
EcoT14I      1590  CCTTGG               C^C[AT][AT]GG
             1763  CCATGG              
FauI          650  CCCGC                CCCGC(4/6)
             1473  GCGGG               
HaeII        1583  AGCGCC               [GA]GCGC^[CT]
             2411  GGCGCC              
MvnI          280  CGCG                 CG^CG
             2552  CGCG                
Psp1406I     1367  AACGTT               AA^CGTT
             1888  AACGTT              
RcaI           48  TCATGA               T^CATGA
             1605  TCATGA              
SmlI          149  CTCAAG               C^T[CT][GA]AG
              994  CTTAAG              
SphI         1501  GCATGC               GCATG^C
             2555  GCATGC              
StuI          293  AGGCCT               AGG^CCT
              500  AGGCCT              
StyI         1590  CCTTGG               C^C[AT][AT]GG
             1763  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        XhoII       XmnI        
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