Restriction map for pF1KB4196
 (Linear) (Six-base) MAPSORT of: KIBB4196.seq  Check: 7062  from: 1  to: 2871



KIBB4196.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     109    2871 
   Size:        109    2762
 
Acc65I G'GTAC_C

Cuts at:      0    1865    2871 
   Size:       1865    1006
 
AclI AA'CG_TT

Cuts at:      0    1368    1889    2871 
   Size:       1368     521     982

  Fragments arranged by size: 

                1368     982     521
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1519    1530    1897    2437    2871 
   Size:        304    1215      11     367     540     434

  Fragments arranged by size: 

                1215     540     434     367     304      11
 
AflII C'TTAA_G

Cuts at:      0     994    2871 
   Size:        994    1877
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2226    2871 
   Size:       2226     645
 
AleI CACnn'nnGTG

Cuts at:      0    1519    1592    2871 
   Size:       1519      73    1279

  Fragments arranged by size: 

                1519    1279      73
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1960    2871 
   Size:       1960     911
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1734    2262    2607    2871 
   Size:        939     795     528     345     264

  Fragments arranged by size: 

                 939     795     528     345     264
 
ApaI G_GGCC'C

Cuts at:      0     469    2871 
   Size:        469    2402
 
ApaLI G'TGCA_C

Cuts at:      0     910    2871 
   Size:        910    1961
 
ApoI r'AATT_y

Cuts at:      0     841     927    2026    2871 
   Size:        841      86    1099     845

  Fragments arranged by size: 

                1099     845     841      86
 
AvaI C'yCGr_G

Cuts at:      0     461    2708    2871 
   Size:        461    2247     163

  Fragments arranged by size: 

                2247     461     163
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1522    1555    1857    1890    2871 
   Size:       1522      33     302      33     981

  Fragments arranged by size: 

                1522     981     302      33      33
 
BamHI G'GATC_C

Cuts at:      0     578    2871 
   Size:        578    2293
 
BanI G'GyrC_C

Cuts at:      0     205    1620    1865    2411    2445    2871 
   Size:        205    1415     245     546      34     426

  Fragments arranged by size: 

                1415     546     426     245     205      34
 
BanII G_rGCy'C

Cuts at:      0     469     776    1673    1757    2871 
   Size:        469     307     897      84    1114

  Fragments arranged by size: 

                1114     897     469     307      84
 
BbeI G_GCGC'C

Cuts at:      0    2415    2871 
   Size:       2415     456
 
BbvCI CC'TCA_GC

Cuts at:      0     749    2060    2407    2441    2871 
   Size:        749    1311     347      34     430

  Fragments arranged by size: 

                1311     749     430     347      34
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      83     117    2871 
   Size:         83      34    2754

  Fragments arranged by size: 

                2754      83      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2530    2871 
   Size:       2530     341
 
BlpI GC'TnA_GC

Cuts at:      0     352     380    1802    2871 
   Size:        352      28    1422    1069

  Fragments arranged by size: 

                1422    1069     352      28
 
Bme1580I G_kGCm'C

Cuts at:      0     469     914    1625    1849    2871 
   Size:        469     445     711     224    1022

  Fragments arranged by size: 

                1022     711     469     445     224
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1667    1722    1752    2444    2819    2871 
   Size:       1667      55      30     692     375      52

  Fragments arranged by size: 

                1667     692     375      55      52      30
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     315    2871 
   Size:        315    2556
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     709     988    1756    1771    2113    2259    2380 
   Size:        508     201     279     768      15     342     146     121

Cuts at:   2380    2566    2714    2871 
   Size:        186     148     157

  Fragments arranged by size: 

                 768     508     342     279     201     186     157     148
                 146     121      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     175     497     749    2060    2407    2441    2871 
   Size:        175     322     252    1311     347      34     430

  Fragments arranged by size: 

                1311     430     347     322     252     175      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134    2871 
   Size:        134    2737
 
BsaAI yAC'GTr

Cuts at:      0    1320    2871 
   Size:       1320    1551
 
BsaHI Gr'CG_yC

Cuts at:      0     976    2113    2412    2871 
   Size:        976    1137     299     459

  Fragments arranged by size: 

                1137     976     459     299
 
BsaWI w'CCGG_w

Cuts at:      0    1868    2387    2871 
   Size:       1868     519     484

  Fragments arranged by size: 

                1868     519     484
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1033    1063    1744    1774    2137    2167    2871 
   Size:       1033      30     681      30     363      30     704

  Fragments arranged by size: 

                1033     704     681     363      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     382     541     550    1041    1044    1390    1426 
   Size:        137     245     159       9     491       3     346      36

Cuts at:   1426    2446    2512    2871 
   Size:       1020      66     359

  Fragments arranged by size: 

                1020     491     359     346     245     159     137      66
                  36       9       3
 
BseYI C'CCAG_C

Cuts at:      0     160     512    1136    1632    2594    2871 
   Size:        160     352     624     496     962     277

  Fragments arranged by size: 

                 962     624     496     352     277     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125    1930    2871 
   Size:        125    1805     941

  Fragments arranged by size: 

                1805     941     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     812     914    1319    1831    2871 
   Size:        812     102     405     512    1040

  Fragments arranged by size: 

                1040     812     512     405     102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2104    2542    2871 
   Size:       2104     438     329

  Fragments arranged by size: 

                2104     438     329
 
Bsp1286I G_dGCh'C

Cuts at:      0     469     776     812     914    1319    1625    1673    1757 
   Size:        469     307      36     102     405     306      48      84

Cuts at:   1757    1831    1849    2871 
   Size:         74      18    1022

  Fragments arranged by size: 

                1022     469     405     307     306     102      84      74
                  48      36      18
 
BspHI T'CATG_A

Cuts at:      0      48    1605    2871 
   Size:         48    1557    1266

  Fragments arranged by size: 

                1557    1266      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     109    1915    2270    2871 
   Size:        109    1806     355     601

  Fragments arranged by size: 

                1806     601     355     109
 
BsrBI CCG'CTC

Cuts at:      0     681    2033    2871 
   Size:        681    1352     838

  Fragments arranged by size: 

                1352     838     681
 
BsrDI GCAATG_nn'

Cuts at:      0     722    1496    1668    1796    2499    2871 
   Size:        722     774     172     128     703     372

  Fragments arranged by size: 

                 774     722     703     372     172     128
 
BsrFI r'CCGG_y

Cuts at:      0    2115    2871 
   Size:       2115     756
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1302    1502    2088    2871 
   Size:       1302     200     586     783

  Fragments arranged by size: 

                1302     783     586     200
 
BstEII G'GTnAC_C

Cuts at:      0    1791    2871 
   Size:       1791    1080
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1278    2871 
   Size:       1278    1593
 
BstYI r'GATC_y

Cuts at:      0     578     783    2360    2561    2871 
   Size:        578     205    1577     201     310

  Fragments arranged by size: 

                1577     578     310     205     201
 
Bsu36I CC'TnA_GG

Cuts at:      0     113    2871 
   Size:        113    2758
 
BtgI C'CryG_G

Cuts at:      0    1763    2871 
   Size:       1763    1108
 
BtsI GCAGTG_nn'

Cuts at:      0    1211    1344    1849    2331    2655    2871 
   Size:       1211     133     505     482     324     216

  Fragments arranged by size: 

                1211     505     482     324     216     133
 
DraIII CAC_nnn'GTG

Cuts at:      0    1411    2871 
   Size:       1411    1460
 
EaeI y'GGCC_r

Cuts at:      0     620    1556    2134    2330    2507    2871 
   Size:        620     936     578     196     177     364

  Fragments arranged by size: 

                 936     620     578     364     196     177
 
EarI CTCTTCn'nnn_

Cuts at:      0     335     793    1549    2871 
   Size:        335     458     756    1322

  Fragments arranged by size: 

                1322     756     458     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     508     709     988    1519    1530    1756    1771 
   Size:        304     204     201     279     531      11     226      15

Cuts at:   1771    1897    2113    2259    2380    2437    2566    2714    2871 
   Size:        126     216     146     121      57     129     148     157

  Fragments arranged by size: 

                 531     304     279     226     216     204     201     157
                 148     146     129     126     121      57      15      11
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2063    2871 
   Size:       2063     808
 
EcoO109I rG'GnC_Cy

Cuts at:      0     465     466     936    2871 
   Size:        465       1     470    1935

  Fragments arranged by size: 

                1935     470     465       1
 
EcoRI G'AATT_C

Cuts at:      0     841     927    2871 
   Size:        841      86    1944

  Fragments arranged by size: 

                1944     841      86
 
HaeII r_GCGC'y

Cuts at:      0    1587    2415    2871 
   Size:       1587     828     456

  Fragments arranged by size: 

                1587     828     456
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     358     390     570     602    1744    1776 
   Size:        113      32     213      32     180      32    1142      32

Cuts at:   1776    2137    2169    2181    2212    2213    2244    2743    2775 
   Size:        361      32      12      31       1      31     499      32

Cuts at:   2775    2871 
   Size:         96

  Fragments arranged by size: 

                1142     499     361     213     180     113      96      32
                  32      32      32      32      32      31      31      12
                   1
 
HincII GTy'rAC

Cuts at:      0     644    1398    1746    1893    2871 
   Size:        644     754     348     147     978

  Fragments arranged by size: 

                 978     754     644     348     147
 
HindIII A'AGCT_T

Cuts at:      0     316    2871 
   Size:        316    2555
 
KasI G'GCGC_C

Cuts at:      0    2411    2871 
   Size:       2411     460
 
KpnI G_GTAC'C

Cuts at:      0    1869    2871 
   Size:       1869    1002
 
MfeI C'AATT_G

Cuts at:      0    1428    2871 
   Size:       1428    1443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     226    2871 
   Size:        226    2645
 
MscI TGG'CCA

Cuts at:      0     622    1558    2136    2332    2871 
   Size:        622     936     578     196     539

  Fragments arranged by size: 

                 936     622     578     539     196
 
MslI CAynn'nnrTG

Cuts at:      0    1216    1506    1519    1592    1618    2492    2871 
   Size:       1216     290      13      73      26     874     379

  Fragments arranged by size: 

                1216     874     379     290      73      26      13
 
MspA1I CmG'CkG

Cuts at:      0      64     384     576     653    1463    1632    2440    2598 
   Size:         64     320     192      77     810     169     808     158

Cuts at:   2598    2871 
   Size:        273

  Fragments arranged by size: 

                 810     808     320     273     192     169     158      77
                  64
 
NaeI GCC'GGC

Cuts at:      0    2117    2871 
   Size:       2117     754
 
NarI GG'CG_CC

Cuts at:      0    2412    2871 
   Size:       2412     459
 
NcoI C'CATG_G

Cuts at:      0    1763    2871 
   Size:       1763    1108
 
NgoMIV G'CCGG_C

Cuts at:      0    2115    2871 
   Size:       2115     756
 
NspI r_CATG'y

Cuts at:      0     436    1136    1505    2559    2871 
   Size:        436     700     369    1054     312

  Fragments arranged by size: 

                1054     700     436     369     312
 
PasI CC'CwG_GG

Cuts at:      0    1070    2871 
   Size:       1070    1801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     939    1136    1734    1764    1769    2305    2871 
   Size:        939     197     598      30       5     536     566

  Fragments arranged by size: 

                 939     598     566     536     197      30       5
 
PfoI T'CCnGG_A

Cuts at:      0    1004    1733    2871 
   Size:       1004     729    1138

  Fragments arranged by size: 

                1138    1004     729
 
PmlI CAC'GTG

Cuts at:      0    1320    2871 
   Size:       1320    1551
 
PspOMI G'GGCC_C

Cuts at:      0     465    2871 
   Size:        465    2406
 
PstI C_TGCA'G

Cuts at:      0    1307    2603    2627    2661    2748    2871 
   Size:       1307    1296      24      34      87     123

  Fragments arranged by size: 

                1307    1296     123      87      34      24
 
PvuII CAG'CTG

Cuts at:      0      64     384    1632    2440    2598    2871 
   Size:         64     320    1248     808     158     273

  Fragments arranged by size: 

                1248     808     320     273     158      64
 
SexAI A'CCwGG_T

Cuts at:      0    1256    2871 
   Size:       1256    1615
 
SfcI C'TryA_G

Cuts at:      0     391     970    1303    1836    2103    2353    2599    2623 
   Size:        391     579     333     533     267     250     246      24

Cuts at:   2623    2657    2744    2871 
   Size:         34      87     127

  Fragments arranged by size: 

                 579     533     391     333     267     250     246     127
                  87      34      24
 
SfoI GGC'GCC

Cuts at:      0    2413    2871 
   Size:       2413     458
 
SmaI CCC'GGG

Cuts at:      0    2710    2871 
   Size:       2710     161
 
SmlI C'TyrA_G

Cuts at:      0     149     994    2871 
   Size:        149     845    1877

  Fragments arranged by size: 

                1877     845     149
 
SphI G_CATG'C

Cuts at:      0    1505    2559    2871 
   Size:       1505    1054     312

  Fragments arranged by size: 

                1505    1054     312
 
StuI AGG'CCT

Cuts at:      0     295     502    2871 
   Size:        295     207    2369

  Fragments arranged by size: 

                2369     295     207
 
StyI C'CwwG_G

Cuts at:      0    1590    1763    2871 
   Size:       1590     173    1108

  Fragments arranged by size: 

                1590    1108     173
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     533    1104    1624    2463    2646    2871 
   Size:        533     571     520     839     183     225

  Fragments arranged by size: 

                 839     571     533     520     225     183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1033    1065    2871 
   Size:       1033      32    1806

  Fragments arranged by size: 

                1806    1033      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1021    1588    1770    2859    2871 
   Size:       1021     567     182    1089      12

  Fragments arranged by size: 

                1089    1021     567     182      12
 
XmaI C'CCGG_G

Cuts at:      0    2708    2871 
   Size:       2708     163
 
XmnI GAAnn'nnTTC

Cuts at:      0    1532    1882    2026    2871 
   Size:       1532     350     144     845

  Fragments arranged by size: 

                1532     845     350     144
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI    AflII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PmlI   PspOMI     PstI    PvuII
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AfeI   AflIII     AgeI     AloI     AscI     AseI
    AsiSI    AvrII     BbsI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI     BsaI    BsaBI    BsiEI    BsiWI     BsmI    BspEI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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