Restriction map for pF1KB3954
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        Asp700I     
Asp718I     AspEI       AviII       BbeI        BbvCI       
BcgI        BfrI        BglII       BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      Bst1107I    
BstBI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflMI       PinAI       
PmeI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SapI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SspBI       
SspI        StuI        SwaI        Van91I      XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII        1345  CGCG                 CG^CG
AcyI         1582  GGCGTC               G[GA]^CG[CT]C
AflIII       3972  ACACGT               A^C[GA][CT]GT
Alw44I       1997  GTGCAC               G^TGCAC
ApaLI        1997  GTGCAC               G^TGCAC
AseI         1162  ATTAAT               AT^TAAT
AsnI         1162  ATTAAT               AT^TAAT
AspI         1685  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2899  CCTAGG               C^CTAGG
BclI         4356  TGATCA               T^GATCA
BlnI         2899  CCTAGG               C^CTAGG
BsaHI        1582  GGCGTC               G[GA]^CG[CT]C
BstAPI       2172  GCAAGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1345  CGCG                 CG^CG
CfoI         3131  GCGC                 GCG^C
EarI         3032  GAAGAG               CTCTTC(1/4)
EcoRI        4195  GAATTC               G^AATTC
EcoT22I      3903  ATGCAT               ATGCA^T
FbaI         4356  TGATCA               T^GATCA
HaeII        3130  GGCGCT               [GA]GCGC^[CT]
HgaI         1583  GCGTC                GACGC(5/10)
HhaI         3131  GCGC                 GCG^C
Hin1I        1582  GGCGTC               G[GA]^CG[CT]C
HinP1I       3131  GCGC                 G^CGC
Ksp632I      3032  GAAGAG               CTCTTC(1/4)
MslI         1126  CACGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1345  CGCG                 CG^CG
NdeI          470  CATATG               CA^TATG
NsiI         3903  ATGCAT               ATGCA^T
PflFI        1685  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1162  ATTAAT               AT^TAAT
SalI         1682  GTCGAC               G^TCGAC
SmaI         1235  CCCGGG               CCC^GGG
Tth111I      1685  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1235  CCCGGG               C^CCGGG
XmaI         1235  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          701  GTAGAC               GT^[AC][GT]AC
             1682  GTCGAC              
BalI         2319  TGGCCA               TGG^CCA
             2778  TGGCCA              
BamHI         172  GGATCC               G^GATCC
             1033  GGATCC              
BbrPI        1334  CACGTG               CAC^GTG
             3269  CACGTG              
BciVI         592  GGATAC               GTATCC(6/5)
              764  GGATAC              
BsaAI        1334  CACGTG               [CT]AC^GT[GA]
             3269  CACGTG              
BsrDI        3612  CATTGC               GCAATG(2/0)
             4125  CATTGC              
BsrFI        1255  GCCGGT               [GA]^CCGG[CT]
             1993  GCCGGT              
BssSI        2378  CTCGTG               CACGAG(-5/-1)
             2630  CTCGTG              
BstEII        361  GGTGACC              G^GT[ACGT]ACC
             2662  GGTAACC             
BstPI         361  GGTGACC              G^GT[ACGT]ACC
             2662  GGTAACC             
Cfr10I       1255  GCCGGT               [GA]^CCGG[CT]
             1993  GCCGGT              
EcoNI        1930  CCTGGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3841  CCTAACCAAGG         
EcoO65I       361  GGTGACC              G^GT[ACGT]ACC
             2662  GGTAACC             
MluNI        2319  TGGCCA               TGG^CCA
             2778  TGGCCA              
MscI         2319  TGGCCA               TGG^CCA
             2778  TGGCCA              
NcoI          893  CCATGG               C^CATGG
             3992  CCATGG              
PaeR7I        974  CTCGAG               C^TCGAG
             3494  CTCGAG              
PmaCI        1334  CACGTG               CAC^GTG
             3269  CACGTG              
PmlI         1334  CACGTG               CAC^GTG
             3269  CACGTG              
SphI         1610  GCATGC               GCATG^C
             2888  GCATGC              
XhoI          974  CTCGAG               C^TCGAG
             3494  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
SbfI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SmlI        Sse8387I    StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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