Restriction map for pF1KB3497
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaWI       BseAI       BsiWI       Bsp1407I    
BspEI       BsrGI       BssHII      BstBI       BstEII      
BstPI       CelII       CpoI        DraIII      DrdI        
Eam1105I    Eco47III    EcoNI       EcoO65I     FbaI        
FseI        FspI        HaeII       Hin1I       KasI        
KspI        MfeI        MluI        MroI        MunI        
NarI        NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PvuI        RsrII       
SacI        SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        414  GGTACC               G^GTACC
AccI         1167  GTATAC               GT^[AC][GT]AC
AccII        1903  CGCG                 CG^CG
AclI         3137  AACGTT               AA^CGTT
AflIII       1487  ACATGT               A^C[GA][CT]GT
AlwNI        2018  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         4175  GGGCCC               GGGCC^C
AseI         3531  ATTAAT               AT^TAAT
AsnI         3531  ATTAAT               AT^TAAT
Asp718I       414  GGTACC               G^GTACC
BcnI         2802  CCCGG                CC^[GC]GG
BsaBI        2192  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          521  GAGACC               GGTCTC(1/5)
BsiEI        2501  CGGCCG               CG[GA][CT]^CG
BsmBI        3924  GAGACG               CGTCTC(1/5)
BspDI        1555  ATCGAT               AT^CGAT
BspLU11I     1487  ACATGT               A^CATGT
BsrBI        3457  GAGCGG               CCGCTC(-3/-3)
BsrFI        3634  GCCGGC               [GA]^CCGG[CT]
BssSI        3574  CACGAG               CACGAG(-5/-1)
Bst1107I     1167  GTATAC               GTA^TAC
BstAPI       3898  GCAAAAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1903  CGCG                 CG^CG
BstXI        4246  CCAGAATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1167  GTATAC               GTA^TAC
Cfr10I       3634  GCCGGC               [GA]^CCGG[CT]
ClaI         1555  ATCGAT               AT^CGAT
DraI          528  TTTAAA               TTT^AAA
EagI         2501  CGGCCG               C^GGCCG
EclXI        2501  CGGCCG               C^GGCCG
Eco52I       2501  CGGCCG               C^GGCCG
EcoRI        4135  GAATTC               G^AATTC
EcoRV        2419  GATATC               GAT^ATC
EcoT22I      3955  ATGCAT               ATGCA^T
HgaI         3877  GCGTC                GACGC(5/10)
HindIII      3072  AAGCTT               A^AGCTT
KpnI          414  GGTACC               GGTAC^C
MamI         2192  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1903  CGCG                 CG^CG
NaeI         3634  GCCGGC               GCC^GGC
NciI         2802  CCCGG                CC^[GC]GG
NgoMIV       3634  GCCGGC               G^CCGGC
NsiI         3955  ATGCAT               ATGCA^T
PaeR7I       1145  CTCGAG               C^TCGAG
PflMI        4063  CCACCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        3531  ATTAAT               AT^TAAT
Psp1406I     3137  AACGTT               AA^CGTT
Van91I       4063  CCACCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1963  TCTAGA               T^CTAGA
XhoI         1145  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI         1822  CCTCAGG              CC^T[ACGT]AGG
             3474  CCTCAGG             
AspHI         550  GAGCAC               G[AT]GC[AT]^C
             1094  GTGCTC              
AvaI          914  CCCGAG               C^[CT]CG[GA]G
             1145  CTCGAG              
BalI          132  TGGCCA               TGG^CCA
             2920  TGGCCA              
BanII        3569  GAGCCC               G[GA]GC[CT]^C
             4175  GGGCCC              
BbvCI        2383  CCTCAGC              CCTCAGC(-5/-2)
             1021  GCTGAGG             
BseRI         404  CTCCTC               GAGGAG(10/8)
             1317  CTCCTC              
BsiHKAI       550  GAGCAC               G[AT]GC[AT]^C
             1094  GTGCTC              
BsoBI         914  CCCGAG               C^[CT]CG[GA]G
             1145  CTCGAG              
Bsu36I       1822  CCTCAGG              CC^T[ACGT]AGG
             3474  CCTCAGG             
CfoI          630  GCGC                 GCG^C
             1904  GCGC                
Eco81I       1822  CCTCAGG              CC^T[ACGT]AGG
             3474  CCTCAGG             
FauI         3287  CCCGC                CCCGC(4/6)
             3372  CCCGC               
HhaI          630  GCGC                 GCG^C
             1904  GCGC                
HinP1I        630  GCGC                 G^CGC
             1904  GCGC                
HpaI         3603  GTTAAC               GTT^AAC
             3768  GTTAAC              
MluNI         132  TGGCCA               TGG^CCA
             2920  TGGCCA              
MscI          132  TGGCCA               TGG^CCA
             2920  TGGCCA              
NcoI         2054  CCATGG               C^CATGG
             4229  CCATGG              
SbfI         1980  CCTGCAGG             CCTGCA^GG
             3754  CCTGCAGG            
ScaI          726  AGTACT               AGT^ACT
             1330  AGTACT              
SpeI         1927  ACTAGT               A^CTAGT
             3091  ACTAGT              
Sse8387I     1980  CCTGCAGG             CCTGCA^GG
             3754  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BanI        BbsI        
BbvI        BciVI       BfaI        BglII       BmyI        
BpmI        BpuAI       BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RcaI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI        
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