Restriction map for pF1KB3497
 (Linear) (Six-base) MAPSORT of: KIBB3497.seq  Check: 8075  from: 1  to: 4311



KIBB3497.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3366    4311 
   Size:       3366     945
 
AccI GT'mk_AC

Cuts at:      0    1168    4311 
   Size:       1168    3143
 
Acc65I G'GTAC_C

Cuts at:      0     414    4311 
   Size:        414    3897
 
AclI AA'CG_TT

Cuts at:      0    3138    4311 
   Size:       3138    1173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     644    1217    1510    1706    2000    3101    4311 
   Size:        143     501     573     293     196     294    1101    1210

  Fragments arranged by size: 

                1210    1101     573     501     294     293     196     143
            
 
AflIII A'CryG_T

Cuts at:      0    1487    4311 
   Size:       1487    2824
 
AleI CACnn'nnGTG

Cuts at:      0    1367    3699    4311 
   Size:       1367    2332     612

  Fragments arranged by size: 

                2332    1367     612
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1385    2839    2949    3852    4311 
   Size:       1385    1454     110     903     459

  Fragments arranged by size: 

                1454    1385     903     459     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2023    4311 
   Size:       2023    2288
 
ApaI G_GGCC'C

Cuts at:      0    4179    4311 
   Size:       4179     132
 
ApoI r'AATT_y

Cuts at:      0     344     368    1337    1549    2314    2341    3505    4135 
   Size:        344      24     969     212     765      27    1164     630

Cuts at:   4135    4311 
   Size:        176

  Fragments arranged by size: 

                1164     969     765     630     344     212     176      27
                  24
 
AseI AT'TA_AT

Cuts at:      0    3532    4311 
   Size:       3532     779
 
AvaI C'yCGr_G

Cuts at:      0     914    1145    4311 
   Size:        914     231    3166

  Fragments arranged by size: 

                3166     914     231
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     970    1003    4311 
   Size:        970      33    3308

  Fragments arranged by size: 

                3308     970      33
 
BanI G'GyrC_C

Cuts at:      0     414     910    1855    4311 
   Size:        414     496     945    2456

  Fragments arranged by size: 

                2456     945     496     414
 
BanII G_rGCy'C

Cuts at:      0    3573    4179    4311 
   Size:       3573     606     132

  Fragments arranged by size: 

                3573     606     132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     445     500    1843    4311 
   Size:        445      55    1343    2468

  Fragments arranged by size: 

                2468    1343     445      55
 
BbvCI CC'TCA_GC

Cuts at:      0    1022    2384    4311 
   Size:       1022    1362    1927

  Fragments arranged by size: 

                1927    1362    1022
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     227     284    2529    4311 
   Size:        227      57    2245    1782

  Fragments arranged by size: 

                2245    1782     227      57
 
BfrBI ATG'CAT

Cuts at:      0    3957    4311 
   Size:       3957     354
 
BglII A'GATC_T

Cuts at:      0     192     619    1267    2930    4311 
   Size:        192     427     648    1663    1381

  Fragments arranged by size: 

                1663    1381     648     427     192
 
Bme1580I G_kGCm'C

Cuts at:      0     915    2788    3385    4179    4311 
   Size:        915    1873     597     794     132

  Fragments arranged by size: 

                1873     915     794     597     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     739    2249    3036    4311 
   Size:        739    1510     787    1275

  Fragments arranged by size: 

                1510    1275     787     739
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1332    4311 
   Size:       1332    2979
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1478    2200    2504    3410    4275    4311 
   Size:       1478     722     304     906     865      36

  Fragments arranged by size: 

                1478     906     865     722     304      36
 
Bpu10I CC'TnA_GC

Cuts at:      0    1022    2384    2812    4311 
   Size:       1022    1362     428    1499

  Fragments arranged by size: 

                1499    1362    1022     428
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1311    3183    4311 
   Size:        901     410    1872    1128

  Fragments arranged by size: 

                1872    1128     901     410
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     515    4311 
   Size:        515    3796
 
BsaBI GATnn'nnATC

Cuts at:      0    2196    4311 
   Size:       2196    2115
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3983    4013    4072    4102    4286    4311 
   Size:       3983      30      59      30     184      25

  Fragments arranged by size: 

                3983     184      59      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     395    1308    4311 
   Size:        395     913    3003

  Fragments arranged by size: 

                3003     913     395
 
BseYI C'CCAG_C

Cuts at:      0     177     875    2787    3384    3467    4311 
   Size:        177     698    1912     597      83     844

  Fragments arranged by size: 

                1912     844     698     597     177      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323    2068    3274    4128    4136    4175    4311 
   Size:        323    1745    1206     854       8      39     136

  Fragments arranged by size: 

                1745    1206     854     323     136      39       8
 
BsiEI CG_ry'CG

Cuts at:      0    2504    4311 
   Size:       2504    1807
 
BsiHKAI G_wGCw'C

Cuts at:      0     554    1098    4311 
   Size:        554     544    3213

  Fragments arranged by size: 

                3213     554     544
 
BsmI GAATG_Cn'

Cuts at:      0    1536    2812    3370    3553    4311 
   Size:       1536    1276     558     183     758

  Fragments arranged by size: 

                1536    1276     758     558     183
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3918    4311 
   Size:       3918     393
 
Bsp1286I G_dGCh'C

Cuts at:      0     554     915    1098    2788    3385    3573    4179    4311 
   Size:        554     361     183    1690     597     188     606     132

  Fragments arranged by size: 

                1690     606     597     554     361     188     183     132
            
 
BspHI T'CATG_A

Cuts at:      0     966    2322    3015    3662    4164    4311 
   Size:        966    1356     693     647     502     147

  Fragments arranged by size: 

                1356     966     693     647     502     147
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1797    1974    1988    3366    4311 
   Size:       1797     177      14    1378     945

  Fragments arranged by size: 

                1797    1378     945     177      14
 
BsrBI CCG'CTC

Cuts at:      0    3459    4311 
   Size:       3459     852
 
BsrDI GCAATG_nn'

Cuts at:      0     116    1112    2495    4311 
   Size:        116     996    1383    1816

  Fragments arranged by size: 

                1816    1383     996     116
 
BsrFI r'CCGG_y

Cuts at:      0    3634    4311 
   Size:       3634     677
 
BssSI C'ACGA_G

Cuts at:      0    3574    4311 
   Size:       3574     737
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3904    4311 
   Size:       3904     407
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4253    4311 
   Size:       4253      58
 
BstYI r'GATC_y

Cuts at:      0     192     619    1267    2930    3257    4311 
   Size:        192     427     648    1663     327    1054

  Fragments arranged by size: 

                1663    1054     648     427     327     192
 
BstZ17I GTA'TAC

Cuts at:      0    1169    4311 
   Size:       1169    3142
 
Bsu36I CC'TnA_GG

Cuts at:      0    1823    3475    4311 
   Size:       1823    1652     836

  Fragments arranged by size: 

                1823    1652     836
 
BtgI C'CryG_G

Cuts at:      0    2054    4087    4229    4311 
   Size:       2054    2033     142      82

  Fragments arranged by size: 

                2054    2033     142      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4115    4311 
   Size:       4115     196
 
BtsI GCAGTG_nn'

Cuts at:      0    2348    2388    3846    4119    4311 
   Size:       2348      40    1458     273     192

  Fragments arranged by size: 

                2348    1458     273     192      40
 
ClaI AT'CG_AT

Cuts at:      0    1556    4311 
   Size:       1556    2755
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1758    1793    4311 
   Size:       1758      35    2518

  Fragments arranged by size: 

                2518    1758      35
 
DraI TTT'AAA

Cuts at:      0     530    4311 
   Size:        530    3781
 
EaeI y'GGCC_r

Cuts at:      0     132    2501    2920    3859    4311 
   Size:        132    2369     419     939     452

  Fragments arranged by size: 

                2369     939     452     419     132
 
EagI C'GGCC_G

Cuts at:      0    2501    4311 
   Size:       2501    1810
 
EarI CTCTTCn'nnn_

Cuts at:      0     620    1193    1370    1376    1529    2070    3884    3916 
   Size:        620     573     177       6     153     541    1814      32

Cuts at:   3916    4311 
   Size:        395

  Fragments arranged by size: 

                1814     620     573     541     395     177     153      32
                   6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      63    4311 
   Size:         63    4248
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     644    1217    1478    1510    1706    2000    2200 
   Size:        143     501     573     261      32     196     294     200

Cuts at:   2200    2504    3101    3410    4275    4311 
   Size:        304     597     309     865      36

  Fragments arranged by size: 

                 865     597     573     501     309     304     294     261
                 200     196     143      36      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2967    3471    4175    4176    4311 
   Size:       2967     504     704       1     135

  Fragments arranged by size: 

                2967     704     504     135       1
 
EcoRI G'AATT_C

Cuts at:      0    4135    4311 
   Size:       4135     176
 
EcoRV GAT'ATC

Cuts at:      0    2421    4311 
   Size:       2421    1890
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1642    4311 
   Size:       1642    2669
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     651     685     717    1192    1224    2035    2067 
   Size:        619      32      34      32     475      32     811      32

Cuts at:   2067    3545    3577    3917    3949    3983    4015    4311 
   Size:       1478      32     340      32      34      32     296

  Fragments arranged by size: 

                1478     811     619     475     340     296      34      34
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     693    3605    3770    3881    4311 
   Size:        693    2912     165     111     430

  Fragments arranged by size: 

                2912     693     430     165     111
 
HindIII A'AGCT_T

Cuts at:      0    3072    4311 
   Size:       3072    1239
 
HpaI GTT'AAC

Cuts at:      0    3605    3770    4311 
   Size:       3605     165     541

  Fragments arranged by size: 

                3605     541     165
 
KpnI G_GTAC'C

Cuts at:      0     418    4311 
   Size:        418    3893
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    4311 
   Size:        796    3515
 
MscI TGG'CCA

Cuts at:      0     134    2922    4311 
   Size:        134    2788    1389

  Fragments arranged by size: 

                2788    1389     134
 
MslI CAynn'nnrTG

Cuts at:      0    1367    1450    2086    2224    2821    3699    4311 
   Size:       1367      83     636     138     597     878     612

  Fragments arranged by size: 

                1367     878     636     612     597     138      83
 
MspA1I CmG'CkG

Cuts at:      0    2378    3290    3307    3388    4120    4311 
   Size:       2378     912      17      81     732     191

  Fragments arranged by size: 

                2378     912     732     191      81      17
 
NaeI GCC'GGC

Cuts at:      0    3636    4311 
   Size:       3636     675
 
NcoI C'CATG_G

Cuts at:      0    2054    4229    4311 
   Size:       2054    2175      82

  Fragments arranged by size: 

                2175    2054      82
 
NdeI CA'TA_TG

Cuts at:      0     144    2652    4068    4311 
   Size:        144    2508    1416     243

  Fragments arranged by size: 

                2508    1416     243     144
 
NgoMIV G'CCGG_C

Cuts at:      0    3634    4311 
   Size:       3634     677
 
NsiI A_TGCA'T

Cuts at:      0    3959    4311 
   Size:       3959     352
 
NspI r_CATG'y

Cuts at:      0    1491    2514    3875    4311 
   Size:       1491    1023    1361     436

  Fragments arranged by size: 

                1491    1361    1023     436
 
PciI A'CATG_T

Cuts at:      0    1487    4311 
   Size:       1487    2824
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4069    4311 
   Size:       4069     242
 
PfoI T'CCnGG_A

Cuts at:      0    3143    4311 
   Size:       3143    1168
 
PsiI TTA'TAA

Cuts at:      0    1630    3782    4311 
   Size:       1630    2152     529

  Fragments arranged by size: 

                2152    1630     529
 
PspOMI G'GGCC_C

Cuts at:      0    4175    4311 
   Size:       4175     136
 
PstI C_TGCA'G

Cuts at:      0    1985    2028    3759    3841    4311 
   Size:       1985      43    1731      82     470

  Fragments arranged by size: 

                1985    1731     470      82      43
 
PvuII CAG'CTG

Cuts at:      0    3307    3388    4120    4311 
   Size:       3307      81     732     191

  Fragments arranged by size: 

                3307     732     191      81
 
SapI GCTCTTCn'nnn_

Cuts at:      0     620    1376    1529    4311 
   Size:        620     756     153    2782

  Fragments arranged by size: 

                2782     756     620     153
 
SbfI CC_TGCA'GG

Cuts at:      0    1985    3759    4311 
   Size:       1985    1774     552

  Fragments arranged by size: 

                1985    1774     552
 
ScaI AGT'ACT

Cuts at:      0     728    1332    4311 
   Size:        728     604    2979

  Fragments arranged by size: 

                2979     728     604
 
SfcI C'TryA_G

Cuts at:      0    1535    1703    1829    1981    2024    2538    3755    3837 
   Size:       1535     168     126     152      43     514    1217      82

Cuts at:   3837    4311 
   Size:        474

  Fragments arranged by size: 

                1535    1217     514     474     168     152     126      82
                  43
 
SmlI C'TyrA_G

Cuts at:      0     880    1145    1326    3198    4311 
   Size:        880     265     181    1872    1113

  Fragments arranged by size: 

                1872    1113     880     265     181
 
SpeI A'CTAG_T

Cuts at:      0    1927    3091    4311 
   Size:       1927    1164    1220

  Fragments arranged by size: 

                1927    1220    1164
 
StyI C'CwwG_G

Cuts at:      0     712     827     844    2054    3614    4229    4311 
   Size:        712     115      17    1210    1560     615      82

  Fragments arranged by size: 

                1560    1210     712     615     115      82      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2264    4311 
   Size:       2264    2047
 
TatI w'GTAC_w

Cuts at:      0     537     726    1330    4311 
   Size:        537     189     604    2981

  Fragments arranged by size: 

                2981     604     537     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     629     661    2945    2977    3322    3354    4072    4104 
   Size:        629      32    2284      32     345      32     718      32

Cuts at:   4104    4311 
   Size:        207

  Fragments arranged by size: 

                2284     718     629     345     207      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0    1963    4311 
   Size:       1963    2348
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     956    2834    4073    4311 
   Size:        956    1878    1239     238

  Fragments arranged by size: 

                1878    1239     956     238
 
XhoI C'TCGA_G

Cuts at:      0    1145    4311 
   Size:       1145    3166
 
XmnI GAAnn'nnTTC

Cuts at:      0     194    1749    1817    3651    4311 
   Size:        194    1555      68    1834     660

  Fragments arranged by size: 

                1834    1555     660     194      68
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI
    AlwNI     ApaI     ApoI     AseI     AvaI     BaeI     BanI    BanII
     BbsI    BbvCI    BciVI    BfrBI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI     PciI
    PflMI     PfoI     PsiI   PspOMI     PstI    PvuII     SapI     SbfI
     ScaI     SfcI     SmlI     SpeI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AloI    ApaLI     AscI
    AsiSI    AvrII    BamHI     BbeI     BcgI     BclI     BglI     BlpI
    BmgBI     BmtI    BsaAI    BsaHI    BsaWI    BsiWI    BspEI    BsrGI
   BssHII    BstBI   BstEII   DraIII     DrdI  EcoICRI    EcoNI     FseI
     FspI    FspAI    HaeII     KasI     MfeI     MluI     NarI     NheI
     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI
    PshAI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XmaI     ZraI
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