Restriction map for pF1KB3038
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        Aor51HI     ApaI        AscI        Asp718I     
AvaI        AviII       BbrPI       BcgI        BciVI       
BclI        BcnI        BglI        BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BspLU11I    BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       FbaI        FseI        FspI        
HapII       HpaII       KpnI        KspI        MamI        
MluI        MroI        MspI        NaeI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII        1441  CGCG                 CG^CG
AflII        2929  CTTAAG               C^TTAAG
AhdI          544  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4082  GTGCAC               G^TGCAC
ApaLI        4082  GTGCAC               G^TGCAC
AseI         2560  ATTAAT               AT^TAAT
AsnI         2560  ATTAAT               AT^TAAT
Asp700I      3531  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         544  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2392  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1071  CCTAGG               C^CTAGG
BamHI        3036  GGATCC               G^GATCC
BbeI          196  GGCGCC               GGCGC^C
BbsI         3884  GTCTTC               GAAGAC(2/6)
BbvCI        2332  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2929  CTTAAG               C^TTAAG
BlnI         1071  CCTAGG               C^CTAGG
BlpI         2675  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2675  GCTCAGC              GC^T[ACGT]AGC
BpuAI        3884  GTCTTC               GAAGAC(2/6)
BsaAI        2909  TACGTG               [CT]AC^GT[GA]
BsmBI        1656  CGTCTC               CGTCTC(1/5)
BsmI          384  GAATGC               GAATGC(1/-1)
BspHI         186  TCATGA               T^CATGA
BsrBI          90  GAGCGG               CCGCTC(-3/-3)
BstAPI        334  GCATTATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1441  CGCG                 CG^CG
BstXI        2007  CCAGGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2675  GCTCAGC              GC^T[ACGT]AGC
Eam1105I      544  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1565  GAATTC               G^AATTC
KasI          196  GGCGCC               G^GCGCC
MvnI         1441  CGCG                 CG^CG
NarI          196  GGCGCC               GG^CGCC
NdeI         2986  CATATG               CA^TATG
PflFI        2392  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2560  ATTAAT               AT^TAAT
RcaI          186  TCATGA               T^CATGA
SacI         2924  GAGCTC               GAGCT^C
SfoI          196  GGCGCC               GGC^GCC
SmlI         2929  CTTAAG               C^T[CT][GA]AG
SphI         2695  GCATGC               GCATG^C
SwaI         1457  ATTTAAAT             ATTT^AAAT
Tth111I      2392  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2363  CCAATAACAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         3531  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          415  GTCTAC               GT^[AC][GT]AC
             1223  GTCTAC              
AcyI          108  GGCGTC               G[GA]^CG[CT]C
              196  GGCGCC              
AocI         1102  CCTCAGG              CC^T[ACGT]AGG
             2114  CCTCAGG             
AspHI        2924  GAGCTC               G[AT]GC[AT]^C
             4082  GTGCAC              
BanII        2589  GAGCCC               G[GA]GC[CT]^C
             2924  GAGCTC              
BsaHI         108  GGCGTC               G[GA]^CG[CT]C
              196  GGCGCC              
BsaI         1530  GAGACC               GGTCTC(1/5)
             3846  GGTCTC              
BsiHKAI      2924  GAGCTC               G[AT]GC[AT]^C
             4082  GTGCAC              
Bsp1407I     1032  TGTACA               T^GTACA
             2878  TGTACA              
BspMI        1826  ACCTGC               ACCTGC(4/8)
             1954  GCAGGT              
BsrGI        1032  TGTACA               T^GTACA
             2878  TGTACA              
Bsu36I       1102  CCTCAGG              CC^T[ACGT]AGG
             2114  CCTCAGG             
EarI         1980  CTCTTC               CTCTTC(1/4)
             2448  CTCTTC              
Eco81I       1102  CCTCAGG              CC^T[ACGT]AGG
             2114  CCTCAGG             
EcoT22I       431  ATGCAT               ATGCA^T
              754  ATGCAT              
FauI         1282  CCCGC                CCCGC(4/6)
               79  GCGGG               
HaeII         141  GGCGCT               [GA]GCGC^[CT]
              196  GGCGCC              
HgaI          109  GCGTC                GACGC(5/10)
             1655  GCGTC               
Hin1I         108  GGCGTC               G[GA]^CG[CT]C
              196  GGCGCC              
HindIII       677  AAGCTT               A^AGCTT
             3859  AAGCTT              
HpaI         2617  GTTAAC               GTT^AAC
             3463  GTTAAC              
Ksp632I      1980  CTCTTC               CTCTTC(1/4)
             2448  CTCTTC              
NsiI          431  ATGCAT               ATGCA^T
              754  ATGCAT              
NspI         1356  ACATGC               [GA]CATG^[CT]
             2695  GCATGC              
SexAI        2307  ACCAGGT              A^CC[AT]GGT
             3429  ACCAGGT             
SspBI        1032  TGTACA               T^GTACA
             2878  TGTACA              
SspI         3259  AATATT               AAT^ATT
             3472  AATATT              
StuI         1016  AGGCCT               AGG^CCT
             3116  AGGCCT              
TaqI          492  TCGA                 T^CGA
             2231  TCGA                
TthHB8I       492  TCGA                 T^CGA
             2231  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BalI        BanI        
BbvI        BfaI        BglII       BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MunI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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