Restriction map for pF1KB1755
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AccI        AclI        AflII       AocI        AscI        
AvrII       BamHI       BbeI        BbrPI       BclI        
BfrI        BlnI        BsaBI       BsiWI       BspLU11I    
Bst1107I    BstBI       BstZ17I     Bsu36I      CpoI        
EagI        EclXI       Eco52I      Eco81I      EcoRV       
FbaI        FseI        HpaI        KasI        KspI        
MamI        MfeI        MunI        NarI        NheI        
NotI        NruI        NspI        NspV        PacI        
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AhdI         2962  GACGGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       305  AGCGCT               AGC^GCT
ApaI         1142  GGGCCC               GGGCC^C
AseI         3151  ATTAAT               AT^TAAT
AsnI         3151  ATTAAT               AT^TAAT
Asp700I       987  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2962  GACGGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1189  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1064  TGCGCA               TGC^GCA
BalI          689  TGGCCA               TGG^CCA
BciVI        1993  GGATAC               GTATCC(6/5)
BfaI         3307  CTAG                 C^TAG
BglI         4378  GCCTCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3809  AGATCT               A^GATCT
BsaAI        1933  TACGTG               [CT]AC^GT[GA]
BsiEI         151  CGGTCG               CG[GA][CT]^CG
BsmI         3325  GCATTC               GAATGC(1/-1)
BssHII        936  GCGCGC               G^CGCGC
BssSI        2272  CACGAG               CACGAG(-5/-1)
BstAPI       2665  GCAGTCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       2823  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2962  GACGGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      305  AGCGCT               AGC^GCT
EcoRI        3985  GAATTC               G^AATTC
EcoT22I      3323  ATGCAT               ATGCA^T
FspI         1064  TGCGCA               TGC^GCA
HindIII      3962  AAGCTT               A^AGCTT
MaeI         3307  CTAG                 C^TAG
MluI         2810  ACGCGT               A^CGCGT
MluNI         689  TGGCCA               TGG^CCA
MscI          689  TGGCCA               TGG^CCA
NaeI         2371  GCCGGC               GCC^GGC
NcoI         1157  CCATGG               C^CATGG
NdeI         3826  CATATG               CA^TATG
NgoMIV       2371  GCCGGC               G^CCGGC
NsiI         3323  ATGCAT               ATGCA^T
PaeR7I       3001  CTCGAG               C^TCGAG
PflFI        1189  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        3151  ATTAAT               AT^TAAT
ScaI         3401  AGTACT               AGT^ACT
SexAI        2824  ACCTGGT              A^CC[AT]GGT
SspI           64  AATATT               AAT^ATT
Tth111I      1189  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3001  CTCGAG               C^TCGAG
XmnI          987  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII       2913  TCCGGA               T^CCGGA
             3955  TCCGGA              
AgeI         1305  ACCGGT               A^CCGGT
             1543  ACCGGT              
AlwNI         204  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              268  CAGTCCCTG           
BlpI         3112  GCTGAGC              GC^T[ACGT]AGC
             4158  GCTCAGC             
Bpu1102I     3112  GCTGAGC              GC^T[ACGT]AGC
             4158  GCTCAGC             
BseAI        2913  TCCGGA               T^CCGGA
             3955  TCCGGA              
Bsp1407I     1055  TGTACA               T^GTACA
             4304  TGTACA              
BspDI         804  ATCGAT               AT^CGAT
             3233  ATCGAT              
BspEI        2913  TCCGGA               T^CCGGA
             3955  TCCGGA              
BspHI        3276  TCATGA               T^CATGA
             3755  TCATGA              
BsrGI        1055  TGTACA               T^GTACA
             4304  TGTACA              
BstEII        855  GGTCACC              G^GT[ACGT]ACC
             4059  GGTGACC             
BstPI         855  GGTCACC              G^GT[ACGT]ACC
             4059  GGTGACC             
BstXI         542  CCATGCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2136  CCATCCTTATGG        
CelII        3112  GCTGAGC              GC^T[ACGT]AGC
             4158  GCTCAGC             
ClaI          804  ATCGAT               AT^CGAT
             3233  ATCGAT              
DraI         2613  TTTAAA               TTT^AAA
             2866  TTTAAA              
EcoNI         860  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1032  CCTGCACAAGG         
EcoO65I       855  GGTCACC              G^GT[ACGT]ACC
             4059  GGTGACC             
HaeII         305  AGCGCT               [GA]GCGC^[CT]
             1099  AGCGCC              
HincII       1675  GTCAAC               GT[CT]^[GA]AC
             2362  GTCAAC              
HindII       1675  GTCAAC               GT[CT]^[GA]AC
             2362  GTCAAC              
MroI         2913  TCCGGA               T^CCGGA
             3955  TCCGGA              
PinAI        1305  ACCGGT               A^CCGGT
             1543  ACCGGT              
RcaI         3276  TCATGA               T^CATGA
             3755  TCATGA              
SacI         2998  GAGCTC               GAGCT^C
             3550  GAGCTC              
SapI          439  GAAGAGC              GCTCTTC(1/4)
             2995  GAAGAGC             
SgrAI        1304  CACCGGTG             C[GA]^CCGG[CT]G
             1542  CACCGGTG            
SspBI        1055  TGTACA               T^GTACA
             4304  TGTACA              
StuI         1010  AGGCCT               AGG^CCT
             2417  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AatII       Acc65I      AccII       AciI        AcsI        
AcyI        AfaI        AflIII      AluI        Alw44I      
AlwI        ApaLI       ApoI        Asp718I     AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcgI        BcnI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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