Restriction map for pF1KB0009
##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
AscI        AseI        AsnI        Asp718I     BbeI        
BciVI       BsaBI       BsiEI       BsiWI       BspDI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
DrdI        EagI        EclXI       Eco52I      EcoRV       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NarI        NdeI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SacII       SalI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SpeI        SspI        SwaI        
XcmI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       4442  TCCGGA               T^CCGGA
AflII        3160  CTTAAG               C^TTAAG
AhdI         4426  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3385  AGCGCT               AGC^GCT
ApaI         1721  GGGCCC               GGGCC^C
AspEI        4426  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3109  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3684  CCTAGG               C^CTAGG
BbrPI        4579  CACGTG               CAC^GTG
BbvCI        3190  GCTGAGG              CCTCAGC(-5/-2)
BclI         2036  TGATCA               T^GATCA
BfrI         3160  CTTAAG               C^TTAAG
BlnI         3684  CCTAGG               C^CTAGG
BseAI        4442  TCCGGA               T^CCGGA
BsmBI         448  GAGACG               CGTCTC(1/5)
Bsp1407I     1632  TGTACA               T^GTACA
BspEI        4442  TCCGGA               T^CCGGA
BspHI         195  TCATGA               T^CATGA
BspLU11I     1922  ACATGT               A^CATGT
BsrGI        1632  TGTACA               T^GTACA
CpoI            5  CGGACCG              CG^G[AT]CCG
DraI         4740  TTTAAA               TTT^AAA
DraIII       1513  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     4426  GACCCGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3385  AGCGCT               AGC^GCT
EcoRI        3499  GAATTC               G^AATTC
EcoT22I      4342  ATGCAT               ATGCA^T
FbaI         2036  TGATCA               T^GATCA
FseI         2877  GGCCGGCC             GGCCGG^CC
MroI         4442  TCCGGA               T^CCGGA
NaeI         2878  GCCGGC               GCC^GGC
NcoI         1662  CCATGG               C^CATGG
NgoMIV       2878  GCCGGC               G^CCGGC
NsiI         4342  ATGCAT               ATGCA^T
PaeR7I        610  CTCGAG               C^TCGAG
PflFI        3109  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4579  CACGTG               CAC^GTG
PmlI         4579  CACGTG               CAC^GTG
RcaI          195  TCATGA               T^CATGA
RsrII           5  CGGACCG              CG^G[AT]CCG
SbfI         2841  CCTGCAGG             CCTGCA^GG
SexAI        1514  ACCAGGT              A^CC[AT]GGT
SnaBI        1767  TACGTA               TAC^GTA
Sse8387I     2841  CCTGCAGG             CCTGCA^GG
SspBI        1632  TGTACA               T^GTACA
Tth111I      3109  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2070  TCTAGA               T^CTAGA
XhoI          610  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII         605  CGCG                 CG^CG
             3313  CGCG                
AcyI         2356  GACGCC               G[GA]^CG[CT]C
             3769  GACGCC              
AflIII        263  ACGTGT               A^C[GA][CT]GT
             1922  ACATGT              
Alw44I        592  GTGCAC               G^TGCAC
             1510  GTGCAC              
AocI         3645  CCTGAGG              CC^T[ACGT]AGG
             4036  CCTGAGG             
ApaLI         592  GTGCAC               G^TGCAC
             1510  GTGCAC              
BglI         1415  GCCAGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2983  GCCTCTGTGGC         
BlpI         1270  GCTGAGC              GC^T[ACGT]AGC
             3388  GCTAAGC             
Bpu1102I     1270  GCTGAGC              GC^T[ACGT]AGC
             3388  GCTAAGC             
BsaAI        1767  TACGTA               [CT]AC^GT[GA]
             4579  CACGTG              
BsaHI        2356  GACGCC               G[GA]^CG[CT]C
             3769  GACGCC              
BsaI         3457  GAGACC               GGTCTC(1/5)
             3886  GAGACC              
BsaWI        3050  ACCGGA               [AT]^CCGG[AT]
             4442  TCCGGA              
BsmI         1086  GAATGC               GAATGC(1/-1)
             3186  GAATGC              
BsrBI        2278  GAGCGG               CCGCTC(-3/-3)
             4456  GAGCGG              
BsrDI        4860  CATTGC               GCAATG(2/0)
             2720  GCAATG              
BstEII       1389  GGTGACC              G^GT[ACGT]ACC
             3609  GGTGACC             
BstPI        1389  GGTGACC              G^GT[ACGT]ACC
             3609  GGTGACC             
BstUI         605  CGCG                 CG^CG
             3313  CGCG                
Bsu36I       3645  CCTGAGG              CC^T[ACGT]AGG
             4036  CCTGAGG             
CelII        1270  GCTGAGC              GC^T[ACGT]AGC
             3388  GCTAAGC             
Eco81I       3645  CCTGAGG              CC^T[ACGT]AGG
             4036  CCTGAGG             
EcoNI        1131  CCTCTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1977  CCTTCAGAAGG         
EcoO65I      1389  GGTGACC              G^GT[ACGT]ACC
             3609  GGTGACC             
Hin1I        2356  GACGCC               G[GA]^CG[CT]C
             3769  GACGCC              
HincII       1096  GTCAAC               GT[CT]^[GA]AC
             3691  GTCAAC              
HindII       1096  GTCAAC               GT[CT]^[GA]AC
             3691  GTCAAC              
MvnI          605  CGCG                 CG^CG
             3313  CGCG                
NheI          630  GCTAGC               G^CTAGC
             2454  GCTAGC              
PflMI         822  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1515  CCAGGTGCTGG         
SapI         1200  GAAGAGC              GCTCTTC(1/4)
              939  GCTCTTC             
SmaI          804  CCCGGG               CCC^GGG
             1718  CCCGGG              
Van91I        822  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1515  CCAGGTGCTGG         
XmaCI         804  CCCGGG               C^CCGGG
             1718  CCCGGG              
XmaI          804  CCCGGG               C^CCGGG
             1718  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcgI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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